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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-Myc-Kif1c-tail-879-1100

Expression vector of mature melanosome marker, which Myc-tagged C terminal half tail region of the mouse Kif1c is expressed.

Catalog number RDB14633
Resource name pEF-Myc-Kif1c-tail-879-1100
Clone info. Expression vector of mature melanosome marker, which Myc-tagged C terminal half tail region (amino acid residues 879-1110) of the mouse kinesin superfamily protein, member 1C (Kif1c) is expressed under the control of the EF1 alpha promoter. Please refer Ishida, M., Sci. Rep. 5, 8238, 2015 (PMID 25649263) for Kif1c-tail domain.
Comment Please refer Mori, Y. et al. J. Biol. Chem., 287 (12): 8963-8973, 2012 (PMID 22291024) for pEF-Myc vector. Expression was confirmed by the deposotor with immunoprecipitation and microscopy.
Vector backbone pEF-Myc (plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name Mouse Kif1c cDNA
Depositor|Developer Fukuda, Mitsunori |
Other clones in our bank
External Database
Mouse Kif1c
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017)
          Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the pEF-BOS and pEF-BOS-EX vectors (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Ishida, M. et al., J. Biochem. 161 (4): 323-326, 2017)。
          付加的提供条件 pEF-BOS and pEF-BOS-EXベクタについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクタを商業的に利用する場合、利用者は長田重一博士に連絡するものとする。利用者は、研究成果の公表にあたってpEF-BOSおよびpEF-BOS-EXベクタの文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14633 pEF-Myc-Kif1c-tail-879-1100 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Ishida, M., M-INK, a novel tool for visualizing melanosomes and melanocores. J. Biochem. 161 (4): 323-326 (2017). PMID 28096452.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0070e (English text)
          Featured content Dnaconda's recommendation EXR0070j (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14633_A7C1p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Region: EF1alpha pro, Myc, insert, hG-CSF p(A)
          Sequence file: RDB14633_A7C1a.seq check
          >D04384A2_A7C1_2_EF1a-C_F_G01_19_ABI24.ab1
              1 CCATGGATGA CTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA CAGTGGTTCA
             61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AGGAATTCTC TAGAGCGGCC GCTCTAGAAC
            121 TAGTGGATCT GCCACCATGG AGCAAAAGCT CATTTCTGAA GAGGACTTGA ATGAAGGATC
            181 TGATGATAAT GACGAGTCTG GTTTGGTCAC CTGGGCCCCA CCGGAAGGGC CAGAAGCAGT
            241 AGAGGAGACA GTGCCCAACG ACCACTCACC AGCTGTCCGG CCCACCTCAC CACCTCTGTC
            301 CAGCTGGGAG CGGGTGTCGA GATTGATGGA AGAGGACCCT GCCTTCCGTC GCGGCCGCCT
            361 TCGCTGGCTC AAGCAGGAGC AGCTGCGGCT GCAGGGACTG CAGGGCGCTG GGGGCCGGGG
            421 TGGGGGACTG CGCAGGCCTC CAGCCCGCTT TGTGCCCCCG CATGATTGCA AGCTGCGCTT
            481 CCCCTTCAAA AGCAACCCTC AGCACAGGGA GTCCTGGCCA GGAATGGGGA GTGGAGAGGC
            541 CCCAGCTCCT CAGCCCCCTG AAGAGGTCAC TGTCCCCCCA GCACCCCCTA ACCGTAGGCC
            601 TCCAAGTCCC CGAAGACCCC ACCGTTCTCG CAGGAATTCC CTAGATGGAG GGAGCCGATC
            661 TCGGGGAGGG GGTTCTACAC AGCCAGAACC CCAGCACTTA CGGCCCCAAA AGCACAACGG
            721 TTATCCCCAA CAGCCCCAGC CCAGCCCAGC CCAGCGGCCA GGGCCCCGCT ACCCACCATA
            781 CACTACCCCT CCGCGAATGC GACGGCAGCG CTCAGCCCCT GACCTCAAAG AGAGTGGGGC
            841 AGCTGTGTGA AATCACTAGT GAATTCGCGG CCGCCTGCAG GTCGACTAAA TCGAATTCCC
            901 GCGGCCGCTC TAGAGTGAGG GTCCCCACCT GGGACCCTTG AGAGTATCAG TCTCCCACGT
            961 GGGAGACAGA ATCCCTGTTT TATATTTAAA CAGCAGTGTT CCCCATCTGG GTCCTTGCAC
           1021 CCCCTCACTC TGGGCTCAGC GACTGCACAG CGGGCCCTGC AATCCGTGAC CTGTGAGTCC
          1081 ATGGACAGTG AGTTACAGAG CCTGGAGGCA TGGATCTTGA G
          //
          Primer: hGCSF_R
          Region: hG-CSF p(A), insert Myc, EF1alpha pro
          Sequence file: RDB14633_A7C1b.seq check
          >D04384A2_A7C1_2_hGCSF_R_H01_22_ABI24.ab1
              1 GGGGGAACTG ATCTCTCAGG GTCCCAGGTG GGGACCCTCA CTCTAGAGCG GCCGCGGGAA
             61 TTCGATTTAG TCGACCTGCA GGCGGCCGCG AATTCACTAG TGATTTCACA CAGCTGCCCC
            121 ACTCTCTTTG AGGTCAGGGG CTGAGCGCTG CCGTCGCATT CGCGGAGGGG TAGTGTATGG
            181 TGGGTAGCGG GGCCCTGGCC GCTGGGCTGG GCTGGGCTGG GGCTGTTGGG GATAACCGTT
            241 GTGCTTTTGG GGCCGTAAGT GCTGGGGTTC TGGCTGTGTA GAACCCCCTC CCCGAGATCG
            301 GCTCCCTCCA TCTAGGGAAT TCCTGCGAGA ACGGTGGGGT CTTCGGGGAC TTGGAGGCCT
            361 ACGGTTAGGG GGTGCTGGGG GGACAGTGAC CTCTTCAGGG GGCTGAGGAG CTGGGGCCTC
            421 TCCACTCCCC ATTCCTGGCC AGGACTCCCT GTGCTGAGGG TTGCTTTTGA AGGGGAAGCG
            481 CAGCTTGCAA TCATGCGGGG GCACAAAGCG GGCTGGAGGC CTGCGCAGTC CCCCACCCCG
            541 GCCCCCAGCG CCCTGCAGTC CCTGCAGCCG CAGCTGCTCC TGCTTGAGCC AGCGAAGGCG
            601 GCCGCGACGG AAGGCAGGGT CCTCTTCCAT CAATCTCGAC ACCCGCTCCC AGCTGGACAG
            661 AGGTGGTGAG GTGGGCCGGA CAGCTGGTGA GTGGTCGTTG GGCACTGTCT CCTCTACTGC
            721 TTCTGGCCCT TCCGGTGGGG CCCAGGTGAC CAAACCAGAC TCGTCATTAT CATCAGATCC
            781 TTCATTCAAG TCCTCTTCAG AAATGAGCTT TTGCTCCATG GTGGCAGATC CACTAGTTCT
            841 AGAGCGGCCG CTCTAGAGAA TTCCTCACGA CACCTGAAAT GGAAGAAAAA AAACTTTGAA
            901 CCACTGTCTG AGGCTTGAGA ATGAACCAAG ATCCAAACTC AAAAAGGGCA AATTCCAAGG
            961 AGAAATTACA TCAAGTGCCC AAGCTGGCCT AACTTCAGTC TCCACCCCCA CTCAGTGTGG
           1021 GAACTCCATC GCATAAAAAC CCCTCCCCCC CAACCTAAAG ACGACGTACT TCCAAAAGGC
           1081 TCGAGAACTA TCGAGTGACT GGAACGCGCC GGTACTCCGT GGAGTCACAT AGAAGCGAAC
          1141 CGGGCC
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Ishida, M., M-INK, a novel tool for visualizing melanosomes and melanocores. J. Biochem. 161 (4): 323-326 (2017). PMID 28096452.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl