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pMXs-IRES-GFP_CA-Akt(E40K)

Retroviral expression vector of constitutively active Akt(E40K)

Catalog number RDB14514
Resource name pMXs-IRES-GFP_CA-Akt(E40K)
Clone info. Retroviral expression vector of constitutively active Akt(E40K), cloned into the pMXs-IRES-GFP Refer Fig.2 of Haniuda, K., Nat. Immunol., 17 (9): 1109-1117 (2016).
Comment Expression was confirmed by the depositor with flow cytometry. Sequence primer: 5term-GGTGGACCATCCTCTAGACT-3term.
Vector backbone pMXs-IRES-GFP (Plasmid)
Selectable markers Amp^r (E. coli)
Gene/insert name mouse Akt1 cDNA
Depositor|Developer Kitamura, Daisuke |
Other clones in our bank

External Database
mouse Akt1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) and an acknowledgment to the DEPOSITOR are requested.
          Additional terms and conditions:
          Regarding CA-Akt E40K: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for CA-Akt E40K (Arimura, Y., J. Biol. Chem. 279 (12), 11408-11416, 2004) and an acknowledgment to Dr. Junji Yagi of Tokyo Women's Meical University are requested.
          Regarding pMXs vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs vector (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Haniuda, K. et al. Nature Immunology 17 (19), 1109-1117, 2016) を引用し、寄託者への謝辞の表明を必要とする。
          付加的提供条件:
          CA-Akt E40Kについて:利用者は、研究成果の公表にあたって CA-Akt E40Kの文献 (Arimura, Y., J. Biol. Chem. 279 (12), 11408-11416, 2004) を引用し、東京女子医科大学八木淳二先生への謝辞の表明を必要とする。
          pMXsベクターについて:利用者は、研究成果の公表にあたって pMXsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄先生への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB14514 pMXs-IRES-GFP_CA-Akt(E40K) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Original reference

          original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14514_A6Lep1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pBABE5'
          Sequence file: RDB14514_A6Lea.seq check
          >D04073B5_A6Le_1_pBABE5'_H01_22_ABI24.ab1
              1 GCCTTAATGG CATATGAGAT CTTATATGGG GCACCCCCGC CCCTTGTAAA CTTCCCTGAC
             61 CCTGACATGA CAAGAGTTAC TAACAGCCCC TCTCTCCAAG CTCACTTACA GGCTCTCTAC
            121 TTAGTCCAGC ACGAAGTCTG GAGACCTCTG GCGGCAGCCT ACCAAGAACA ACTGGACCGA
            181 CCGGTGGTAC CTCACCCTTA CCGAGTCGGC GACACAGTGT GGGTCCGCCG ACACCAGACT
            241 AAGAACCTAG AACCTCGCTG GAAAGGACCT TACACAGTCC TGCTGACCAC CCCCACCGCC
            301 CTCAAAGTAG ACGGCATCGC AGCTTGGATA CACGCCGCCC ACGTGAAGGC TGCCGACCCC
            361 GGGGGTGGAC CATCCTCTAG ACTGCCGGAT CCGGCCACGG ATACCATGAA CGACGTAGCC
            421 ATTGTGAAGG AGGGCTGGCT GCACAAACGA GGGGAATATA TTAAAACCTG GCGGCCACGC
            481 TACTTCCTCC TCAAGAACGA TGGCACCTTT ATTGGCTACA AGAAACGGCC TCAGGATGTG
            541 GATCAGCGAG AGTCCCCACT CAACAACTTC TCAGTGGCAC AATGCCAGCT GATGAAGACA
            601 GAGCGGCCAA GGCCCAACAC CTTTATCATC CGCTGCCTGC AGTGGACCAC AGTCATTGAG
            661 CGCACCTTCC ATGTGGAAAC GCCTGAGGAG CGGGAAGAAT GGGCCACCGC CATTCAGACT
            721 GTGGCAGATG GACTCAAGAG GCAGGAAGAA GAGACGATGG ACTTCCGATC AGGCTCACCC
            781 AGTGACAACT CAGGGGCTGA AGAGATGGAG GTGTCCCTGG CCAAGCCCAA GCACCGTGTG
            841 ACCATGAACG AGTTTGAGTA CCTGAAGCTA CTGGGCAAGG GCACCTTTGG GAAGGTGATT
            901 CTGGTGAAAG AGAAGGCCAC AGGCCGCTAC TATGCCATGA AGATCCTCAA GAAGGAGGTC
            961 ATCGTCGCCC AAGGATGAGG TTGCCCACAC GCTTACTGAG AACCGTGTCC TGCAGAACTC
           1021 TAGGCATCCC TTTCCTTTAC GGCCCTCAAG TACTCATTCC AGACCCACGA CCGCCTCTGC
           1081 TTTGTCATGA GTATGCCCAA CGGGGCGAGC TCTTCGTCAA CTGTCTCGAG AGCGTGTGTT
          1141 TCCTTCGGAG GAACCGGGGT C
          //
          Primer: IRES-R
          Sequence file: RDB14514_A6Leb.seq check
          >D04073B5_A6Le_1_IRES-R_F03_18_ABI24.ab1
              1 GCTCAGCGGC TTCGGCAGTA CGTTAGGGGG GGGGGAGGGA GAGGGGGGGG GGGGGCGGAA
             61 TTTACGTAGC GGCCGCGGCG CGCCGGCCCT CGAGGCCTGC AGGAATTCGG CCTCAGGCTG
            121 TGCCACTGGC TGAGTAGGAG AACTGGGGGA AGTGCGGCCT CCGCTCACTG TCCACACACT
            181 CCATGCTGTC ATCTTGATCA GGCGGCGTGA TGGTGATCAT CTGAGCTGTG AACTCCTCAT
            241 CGAAATACCT GGTGTCAGTC TCAGAGGTGA CCTGGGGCTT GAAAGGTGGG CTCAGCTTCT
            301 TCTCATACAC ATCCTGCCAC ACGATGTTGG CAAAGAACCG GTGCTGCATG ATCTCCTTGG
            361 CATCCTCGGA GCCCCCACCG AGCCTCTGTG TAGGGTCCTT CTTGAGCAGC CCGGAGAGCA
            421 GGGACTTGGC CTCAGGGCCG AGTGTGCGCG GGAAGCGGAT CTCCTCCATG AGGATCAGCT
            481 CGAACAGCTT CTCGTGGTCC TGGTTGTAGA AGGGCAGGCG GCCACACATC ATCTCGTACA
            541 TGACCACGCC CAGCCCCCAC CAGTCCACTG CACGGCCGTA GTCGTTGTCC TCCAGCACCT
            601 CAGGGGCCAG GTACTCCGGC GTTCCGCAGA ATGTCCTCAT AGTGGCACCG TCCTTGATCC
            661 CCTCCTTGCA CAGCCCGAAG TCCGTTATCT TGATGTGCCC GTCCTTGTCC AGCATGAGGT
            721 TCTCCAGCTT CAGGTCCCGG TACACCACGT TCTTCTCGGA GTGCAAGTAG TCCAGGGCAG
            781 ACACAATCTC CGCACCATAG AAGCGGGCCC GGTCCTCGGA GAACACACGC TCTCGAGACA
            841 GGTGGAAGAA GAGCTCGCCC CCGTTGGCAT ACTCCATGAC AAAGCAGAGG CGGTCGTGGG
            901 TCTGGAAATG AGTACTTGAG GGCCGTAAGG GAAGGGATGC CTAGAGTTCT GCAGGACACG
            961 GGTTCTCAGT AAGCGTGTGG GCAACCTCAT CCTTGGCGAC GATGACCTCT TCTGAGATCT
           1021 TCATTGCATA GTAGCGGCTG TGCTTCTCTT TCACAGATCA CTTACTAAAG TGCCCTGGCC
          1081 AGTAGCTCAG TACTCACTCG ATCATGTCAC ACGGTTGCTT GGGCCTG
          //
          Primer: IRES-F
          Sequence file: RDB14514_A6Lec.seq check
          >D04073B5_A6Le_1_IRES-F_H12_24_ABI24.ab1
              1 GGGATTCCTT TATGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG
             61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGT GAGCAAGGGC
            121 GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC
            181 CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG
            241 AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG
            301 ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC
            361 AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC
            421 AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG
            481 CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC
            541 TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA AGAACGGCAT CAAGGTGAAC
            601 TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC TCGCCGACCA CTACCAGCAG
            661 AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA ACCACTACCT GAGCACCCAG
            721 TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA TGGTCCTGCT GGAGTTCGTG
            781 ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA AGTAAAGCGG CCGGCCGCCA
            841 GCACAGTGGT CGACGATAAA ATAAAAGATT TTATTTAGTC TCCAGAAAAA GGGGGGAATG
            901 AAAGACCCCA CCTGTAGGTT TGGGCAAGCT AGCTTAAGTA ACGCCCATTT TGCAAGCATG
            961 GGAAAAAATA CATAACTGGA GAATAGAGAA GTTCAGATCA AGGGTTCAAG GAACAGAATG
           1021 GAACAGCCTG AATAATGGGT CCAAACCAGG AATATCTGGT TGATAAGCAA GTTTCTTGCC
          1081 CCGGCTTCAA AGTCCAGAAC GGATTGAACA GCCTTGGAAT ATGGGGCGCC CAAACAG
          //
          Primer: Amp_R
          Sequence file: RDB14514_A6Led.seq check
          >D04073B5_A6Le_1_Amp_R_G03_21_ABI24.ab1
              1 CTTCGTTTTC ATATTATTGA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT
             61 TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC
            121 ACCTGCATAA TGAAAGACCC CACCTGTAGG TTTGGCAAGC TAGCTTAAGT AACGCCATTT
            181 TGCAAGGCAT GGAAAAATAC ATAACTGAGA ATAGAAAAGT TCAGATCAAG GTCAGGAACA
            241 GATGGAACAG CTGAATATGG GCCAAACAGG ATATCTGTGG TAAGCAGTTC CTGCCCCGGC
            301 TCAGGGCCAA GAACAGATGG AACAGCTGAA TATGGGCCAA ACAGGATATC TGTGGTAAGC
            361 AGTTCCTGCC CCGGCTCAGG GCCAAGAACA GATGGTCCCC AGATGCGGTC CAGCCCTCAG
            421 CAGTTTCTAG AGAACCATCA GATGTTTCCA GGGTGCCCCA AGGACCTGAA ATGACCCTGT
            481 GCCTTATTTG AACTAACCAA TCAGTTCGCT TCTCGCTTCT GTTCGCGCGC TTCTGCTCCC
            541 CGAGCTCAAT AAAAGAGCCC ACAACCCCTC ACTCGGCGCG CCAGTCCTCC GATTGACTGA
            601 GTCGCCCGGG TACCCGTGTA TCCAATAAAC CCTCTTGCAG TTGCATCCGA CTTGTGGTCT
            661 CGCTGTTCCT TGGGAGGGTC TCCTCTGAGT GATTGACTAC CCGTCAGCGG GGGTCTTTCA
            721 TTTGGGGGCT CGTCCGGGAT CGGGAGACCC CTGCCCAGGG ACCACCGACC CACCACCGGG
            781 AGGTAAGCTG GCCAGCAACT TATCTGTGTC TGTCCGATTG TCTAGTGTCT ATGACTGATT
            841 TTATGCGCCT GCGTCGGTAC TAGTTAGCTA ACTAGCTCTG TATCTGGCGG ACCCGTGGTG
            901 GAACTGACGA GTTCGGAACA CCCGGCCGCA ACCCTGGGAG ACGTCCCAGG GACTTCGGGG
            961 GCCGTTTTTG TGGCCCGACC TGAGTCCAAA AATCCCGATC GTTTTGGACT CTTTGGTGCA
           1021 CCCCCCCTTA GAGGAGGATA TGTGGATCTG GTAGGAGACG AGACCTAAAC AGGTCCGCTT
           1081 CGTCCTGAGT CTGCTTCGGC TTTGGAACGG AAGCCGCGAC CGCGCGATCT TTGTTCTTTG
          1141 ACTTGA
          //
          Primer: SV40pA3'element Reverse
          Sequence file: RDB14514_A6Lee.seq check
          >D04073B5_A6Le_1_SV40pA3'element Reverse_H03_24_ABI24.ab1
              1 GATGGTAGGG GGGCGGAGCT ATGGAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC
             61 CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA
            121 CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG
            181 CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC AAACCGCCTC TCCCCGCGCG
            241 TTGGCCGATT CATTAATGCA ACTATGGCCA TTTAATGTAA ATACTTAAGA AAAAAAACCA
            301 AATTAATTTT GATACATGCT GCATGTGAAA GACCCCCGCT GACGGGTAGT CAATCACTCA
            361 GAGGAGACCC TCCCAAGGAA CAGCGAGACC ACAAGTCGGA TGCAACTGCA AGAGGGTTTA
            421 TTGGATACAC GGGTACCCGG GCGACTCAGT CAATCGGAGG ACTGGCGCCC CGAGTGAGGG
            481 GTTGTGGGCT CTTTTATTGA GCTCGGGGAG CAGAAGCGCG CGAACAGAAG CGAGAAGCGA
            541 ACTGATTGGT TAGTTCAAAT AAGGCACAGG GTCATTTCAG GTCCTTGGGG CACCCTGGAA
            601 ACATCTGATG GTTCTCTAGA AACTGCTGAG GGCTGGACCG CATCTGGGGA CCATCTGTTC
            661 TTGGCCCTGA GCCGGGGCAG GAACTGCTTA CCACAGATAT CCTGTTTGGC CCATATTCAG
            721 CTGTTCCATC TGTTCTTGGC CCTGAGCCGG GGCAGGAACT GCTTACCACA GATATCCTGT
            781 TTGGCCCATA TTCAGCTGTT CCATCTGTTC CTGACCTTGA TCTGAACTTC TCTATTCTCA
            841 GTTATGTATT TTTTCCATGC CTTGCAAAAT GGCGTTACTT AAGCTAGCTT GCCAAACCTA
            901 CAGGTGGGGT CTTTCATTCC CCCCCTTTTT TCTGGAGACT AAATAAAATC TTTTATTTTA
            961 TCGTCGACCA CTGTGCTGGG CGGCCGGGCC GCTTTACTTG TACAGCTCGT CCATGCCCGA
           1021 GAGTGATCCC GGCGCGTCAC GACTCCAGCA GGAACAATGT GATCGCGCTT CTCGTTGGGT
          1081 CTTTGCTCAA GTCGGACTGG GTGCCTCCAA GGTTAGGTTG GGT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Haniuda, K., Autonomous membrane IgE signaling prevents IgE-memory formation. Nat. Immunol., 17 (9): 1109-1117 (2016). PMID 27428827.

          2023.04.10

          GNP_filter3_RDBDEP_html_230405.pl