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pCMV-NICD 3xFLAG

Expression vector of mouse Notch1 active form N1deltaECD.

Catalog number RDB14500
Resource name pCMV-NICD 3xFLAG
Alternative name pYY554-1, N1deltaECD
Clone info. Expression vector of mouse Notch1 active form. N1deltaECD in the Fig.4 of Sasaki, N., Development 138 (1); 55-64, 2011.
Comment Nucleotide sequence of 5372 to 7857nt of NM_008714.3 (1..9497nt) corresponding Ile of 1704aa to 2531aa in Fig.1 of Kato, H., FEBS Lett., 395, 221-224 (1996). Known difference: nucleotide substitution of G to A at 7159nt of NM_008714.3 (Glu at 2299aa to Lys).
Vector backbone p3xFLAG-CMV-14 (Plasmid)
Size of vector backbone 6.3 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cells).
Gene/insert name Mouse Notch gene homolog 1 cDNA
Depositor|Developer Saga, Yumiko |
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External Database
Mouse Notch gene homolog 1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Sasaki, N., Development 138, 55-64, 2011) and an acknowledgment to the DEPOSITOR are requested.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Sasaki, N., Development 138, 55-64, 2011)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
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          RDB14500 pCMV-NICD 3xFLAG DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Sasaki, N., The repression of Notch signaling occurs via the destabilization of mastermind-like 1 by Mesp2 and is essential for somitogenesis. Development 138 (1): 55-64 (2011). PMID 21098559.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14500_A6Lcp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro, insert 5'
          Sequence file: RDB14500_A6Lca.seq check
          >D04132A1_A6Lc_1_CMV-Forward_A10_01_ABI08.ab1
              1 CTTTTATTTC CCCCGCCCGT TGACGCAATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
             61 ATAAGCAGAG CTCGTTTAGT GAACCGTCAG AATTAAGCTT GCGGCCGCGA ATTCCCACCA
            121 TGGATATTCC TTACAAGATT GAGGCCGTGA AGAGTGAGCC GGTGGAGCCT CCGCTGCCCT
            181 CGCAGCTGCA CCTCATGTAC GTGGCAGCGG CCGCCTTCGT GCTCCTGTTC TTTGTGGGCT
            241 GTGGGGTGCT GCTGTCCCGC AAGCGCCGGC GGCAGCATGG CCAGCTCTGG TTCCCTGAGG
            301 GTTTCAAAGT GTCAGAGGCC AGCAAGAAGA AGCGGAGAGA GCCCCTCGGC GAGGACTCAG
            361 TCGGCCTCAA GCCCCTGAAG AATGCCTCAG ATGGTGCTCT GATGGACGAC AATCAGAACG
            421 AGTGGGGAGA CGAAGACCTG GAGACCAAGA AGTTCCGGTT TGAGGAGCCA GTAGTTCTCC
            481 CTGACCTGAG TGATCAGACT GACCACAGGC AGTGGACCCA GCAGCACCTG GACGCTGCTG
            541 ACCTGCGCAT GTCTGCCATG GCCCCAACAC CGCCTCAGGG GGAGGTGGAT GCTGACTGCA
            601 TGGATGTCAA TGTTCGAGGA CCAGATGGCT TCACACCCCT CATGATTGCC TCCTGCAGTG
            661 GAGGGGGCCT TGAGACAGGC AACAGTGAAG AAGAAGAAGA TGCACCTGCT GTCATCTCTG
            721 ACTTCATCTA CCAGGGCGCC AGCTTGCACA ACCAGACAGA CCGCACCGGG GAGACCGCCT
            781 TGCACTTGGC TGCCCGATAC TCTCGTTCAG ATGCTGCAAA GCGCTTGCTG GAGGCCAGTG
            841 CAGATGCCAA CATCCAGGAC AACATGGGGC CGTACTCCGT TACATGCAGC AGTTTCTGCA
            901 GATGCTCAGG GTGTCTTCCA GATCCTGCTC CGGAACAGGG CCACAGATCT GGATGCCCGA
            961 ATGCATGATG GCACAACTCC ACTGATCCCT GGGCTGCGCG CCTGGGCGTG GGAGGGCCAT
           1021 GCTGGAAGGA CCTCATCAAC CTCACATGCT GACGTCCAAT GCCGTGATGG ACCTTAGGCA
          1081 AGTCCGCTTG GCCATGGCCC GGACCGGCGG TGACCAAATG TGTGGGAT
          //
          Primer: hGHpolyA-R
          Region: hGH p(A), 3xFLAG, insert 3'
          Sequence file: RDB14500_A6Lcb.seq check
          >D04132A1_A6Lc_1_hGHpolyA-R_B10_02_ABI08.ab1
              1 ACTAACCCCC CTCACCCCAA TAATATTATA GGAAGGACAC CTAGTCAGAC AAAATGATGC
             61 AACTTAATTT TATTAGGACA AGGCTGGTGG GCACTGGAGT GGCAACTTCC AGGGCCAGGA
            121 GAGGCACTGG GGAGGGGTCA CAGGGATGCC ACCCGGGATC ACTACTTGTC ATCGTCATCC
            181 TTGTAGTCGA TGTCATGATC TTTATAATCA CCGTCATGGT CTTTGTAGTC AGCCCGGGAT
            241 CCTTTAAATG CCTCTGGAAT GTGGGTGATC TGGGACGGCA TGGTGGTGGG CGGGCTGGAG
            301 ATGCCCTCGG ACCAATCAGA GATGTTGGAA TGCGGGGAGG AGCTGGACCA CTGGTCAGGG
            361 GACTCAGGGG ATGGGGTGAG GAAGGGGTGC TCTGGCACCT GCAGCTGGTG GCTGGGGGTG
            421 TTGTCCACAG GGGAGGAGGA GTAACTGTGC TGGGAAGGAG GGGTCAGGAA CTGGGTAGTG
            481 GTCATGGGTG GGACCATGGA GGATGGCAGT GATGTGGGCA GGGCCTGGCT TTCCTGGGGC
            541 AGAATGGTGT GCACAGGCAG ACTGCTGGGG CCCAGCGGTT GTACATCTGC CTGACTGGGC
            601 TCCCCACTCA AGAAGCTCCG GCCCAGGTGC CCATTGGCTG CCGAGCTCAC ACTGAGGTGT
            661 GGCTGTGATG GTGGCTGCAG GTTCTGAGGC TGGAGCTGTA AGTTCTGTGG CTGCACCTGC
            721 TGGGTCTGCA CCAGGTGAGG CTGTGTTGCC AGCCGTGTGT TGGGCAGGCC CTGGTAAATA
            781 ATCGGGGACA AGGTATTGGT GGAGAGGCTG CTGTGTAGTG GCCCCATCAT GCTATGCTGG
            841 AGGCCAGCTG CCTGTGTGCT CAGTGTGCCC GGCGTCACAC CCGGCCGTAG TGGGTTGTAC
            901 TGGGCTGGGC ACCATGCCAT TCTGGAGCCG GGAAAGCCAC TTACACTGGC CATTCAAGGC
            961 TTGCCGGTGC ACCCACGGTG GAATTTCATA GCCCCCGTGC CATTGGTACT TCAGCACTGT
           1021 GCTGGCACTG GGAAGGCTAC AGCAAGGTGG AGAGGCCGTT GGCGGGGATG GCTCAAAGCC
          1081 AATAGGCTTA CCTCCAGTCA GTGCTGGCCA ATCTCAAGGC TGGCCTTGGG CCCACCAT
          //
          Primer: RDB14500#1
          Region: insert mid
          Sequence file: RDB14500_A6Lcc.seq check
          >D04132A1_A6Lc_1_RDB14500#1_A04_01_ABI08.ab1
              1 CGGGCAGCTG CCTTGGGCTG CCGATACTCT CGTTCAGATG CTGCAAAGCG CTTGCTGGAG
             61 GCCAGTGCAG ATGCCAACAT CCAGGACAAC ATGGGCCGTA CTCCGTTACA TGCAGCAGTT
            121 TCTGCAGATG CTCAGGGTGT CTTCCAGATC CTGCTCCGGA ACAGGGCCAC AGATCTGGAT
            181 GCCCGAATGC ATGATGGCAC AACTCCACTG ATCCTGGCTG CGCGCCTGGC CGTGGAGGGC
            241 ATGCTGGAGG ACCTCATCAA CTCACATGCT GACGTCAATG CCGTGGATGA CCTAGGCAAG
            301 TCGGCTTTGC ATTGGGCGGC CGCGGTGAAC AATGTGGATG CTGCTGTTGT GCTCCTGAAG
            361 AACGGAGCCA ACAAGGACAT GCAGAACAAC AAGGAGGAGA CTCCCCTGTT CCTGGCCGCC
            421 CGTGAGGGCA GCTATGAGAC TGCCAAAGTG TTGCTGGACC ACTTTGCCAA CCGGGACATC
            481 ACGGATCACA TGGACCGATT GCCGCGGGAC ATCGCACAGG AGCGTATGCA CCACGATATC
            541 GTGCGGCTTT TGGATGAGTA CAACCTGGTG CGCAGCCCAC AGCTGCATGG CACTGCCCTG
            601 GGTGGCACAC CCACTCTGTC TCCCACACTC TGCTCGCCCA ATGGCTACCT GGGCAATCTC
            661 AAGTCTGCCA CACAGGGCAA GAAGGCCCGC AAGCCCAGCA CCAAAGGGCT GGCTTGTGGT
            721 AGCAAGGAAG CTAAGGACCT CAAGGCACGG AGGAAGAAGT CCCAGGATGG CAAGGGCTGC
            781 CTGTTGGACA GCTCGAGCAT GCTGTCGCCT GTGGACTCCC TCGAGTCACC CCATGGCTAC
            841 TTGTCAGATG TGGCCTCGCC ACCCCTCCTC CCCTCCCCAT TCCAGCAGTC TCCATCCATG
            901 CCTCTCAGCC ACCTGCCCTG GTATGCCTGA CACTCACCCT GGGCATCAGC CACTTGAAAT
            961 GTGGCAGCCC AAGCCCTGAG ATGGGCAGCA CTGGGCTGGG AGGGTAGCCG GCTTGGCCTT
           1021 TGAGCCACCC CCCGACACGC TCCTCCCACT GCCTGTAGCT CAGTGCCAGC ACAGTGCTGA
          1081 GTACTAATGC ACGGGGCTAT GGATTCACGT GATGACACCG GACTGCAGGA CCT
          //
          Primer: RDB14500#2
          Region: insert mid
          Sequence file: RDB14500_A6Lcd.seq check
          >D04132A1_A6Lc_1_RDB14500#2_B04_02_ABI08.ab1
              1 CTTTAGGGTG GGGAGGGCTG CTGTGTAGTG GCCCCATCAT GCTATGCTGG AGGCCAGCTG
             61 CCTGTGTGCT CAGTGTGCCC GGCGTCACAC CCGGCCGTAG TGGGTTGTAC TGGCTGGGCA
            121 CCATGCCATT CTGGAGCCGG GGAAGCCACT TACACTGGCC ATTCAAGCTT GCCGGTGCAC
            181 CCACGGTGAA ATTCATAGCC CCCGTGCCAT TGGTACTCAG CACTGTGCTG GCACTGGAGG
            241 CTACAGGCAG GTGGGAGAGG CGTGGCGGGG GTGGCTCAAA GGCCAACCGG CTACCTCCAG
            301 CCAGTGCTGC CATCTCAGGC TTGGCTGCCA CATTCAAGTG GCTGATGCCC AGGTGAGTGT
            361 CAGGCATACC AGGCAGGTGG CTGAGAGGCA TGGATGGAGA CTGCTGGAAT GGGGAGGGGA
            421 GGAGGGGTGG CGAGGCCACA TCTGACAAGT AGCCATGGGG TGACTCGAGG GAGTCCACAG
            481 GCGACAGCAT GCTCGAGCTG TCCAACAGGC AGCCCTTGCC ATCCTGGGAC TTCTTCCTCC
            541 GTGCCTTGAG GTCCTTAGCT TCCTTGCTAC CACAAGCCAG CCCTTTGGTG CTGGGCTTGC
            601 GGGCCTTCTT GCCCTGTGTG GCAGACTTGA GATTGCCCAG GTAGCCATTG GGCGAGCAGA
            661 GTGTGGGGAG ACAGAGTGGG TGTGCCACCC AGGGCAGTGC CATGCAGCTG TGGGCTGCGC
            721 ACCAGGGTTG TACTCATCCA AAAAGCCGCA CGATATCGTG GTGCATACGC TCCTGGTGCG
            781 ATGTCCCGCG GCAATCGGTC CATGTGATCC GTGATGTCCC GGTTGGCAAA GTGGTCCAGC
            841 AACACTTTGG GCAGTCTCAT AGCTGCCCTC ACGGGCGGCC AGGAAACAGG GGAGTCTCCT
            901 TCCTTGTTGT TCTGCCATGT CCTTGTTGGC TCCGTTTCTT CAAGGAGCAC AACAGCAGCA
            961 ATCCCAACAT TTGGTTCCAC CCGCGGCCCG CCCAAATGCA AAAGCCCGAA CTTTGGCCCT
           1021 TAGGGTCATC CACGGGCCAT TTGAACGGTT CAAGCAATGG TTGAAGTTGG ATGGAGGGTC
           1081 CCCTTCCAAG CAATTGCTCC TTTCCAACTG GGCCAGGGGC CGCGGCTCGA AGCCAAGGAT
          1141 TCCAGGTTGG GAAGTTGTGT GGCCCCATTC CATATGGGAC TAATTCCTGG
          //
          Primer: SV40pro_F_V2
          Region: NeoR
          Sequence file: RDB14500_A6Lce.seq check
          >D04132A1_A6Lc_1_SV40pro_F_V2_C04_03_ABI08.ab1
              1 GTTGCGCCTT TTTTGGAGGA TTGATCAGCT TGGGATCTGA TCAAGAGACA GGATGAGGAT
             61 CGTTTCGCAT GATCTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG
            121 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC
            181 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG
            241 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC
            301 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG
            361 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT
            421 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG
            481 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT
            541 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC
            601 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG
            661 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC
            721 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT
            781 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT
            841 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGTT CGAAATGACC GACCAAGCGA
            901 CGCCCAACCT GCCATCACGA GATTTCGATT CCACCGCCGC CCTTCTATGG AAAGGTTGGG
            961 CTTCGGAATT CGTTTTCCGG GACGCCGGCT GGATGGATCC CTCAGCGCGG GGATCTCAAT
           1021 GCTGAAGTTC CTTCGCCCCA CCCCGGCTTC GATCCCTCCG CGAGGTTGGA TCAGCTGCTG
          1081 CTGAAGCCTG ACGACCCTCG CGGGAAGTCA T
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Sasaki, N., The repression of Notch signaling occurs via the destabilization of mastermind-like 1 by Mesp2 and is essential for somitogenesis. Development 138 (1): 55-64 (2011). PMID 21098559.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl