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pcDNA6/hBACE1 deltaTM-myc-His

Expression vector of C-terminal Myc and 6xHis tagged human BACE1 (soluble BACE1).

Catalog number RDB14343
Resource name pcDNA6/hBACE1 deltaTM-myc-His
Alternative name pcDNA6-myc-His A/human BACE1
Clone info. Expression vector of C-terminal Myc and 6xHis tagged human BACE1 (soluble BACE1). This clone is constructed to express only ectodomain of the BACE1 501AA isoform ranging from Met (1AA) to Ser (453AA).
Comment Expression was confirmed by the depositor with western blotting and coomassie brilliant blue (CBB) staining.
Vector backbone pcDNA6/myc-His A (plasmid)
Size of vector backbone 5.1 kb
Selectable markers Amp^r; Blastcidin^r
Gene/insert name human BACE1 cDNA
Depositor|Developer Taniguchi, Naoyuki | Kizuka, Yasuhiko |
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External Database
human BACE1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kizuka, Y. et al., Biochem. J., 473 (1), 21-30, 2016)。
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          RDB14343 pcDNA6/hBACE1 deltaTM-myc-His DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14343_A6Itp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB14343_A6Ita.seq check
          >D03881A1_A6It_1_CMV-Forward_A11_01_ABI08.ab1
              1 CCGAACACTC CGCCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA
             61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA
            121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGTTAAGCTT GGTACCGAGC TCGGATCCAC
            181 TAGTCCAGTG TGGTGGAATT CTGCAGATGC CACCATGGCC CAAGCCCTGC CCTGGCTCCT
            241 GCTGTGGATG GGCGCGGGAG TGCTGCCTGC CCACGGCACC CAGCACGGCA TCCGGCTGCC
            301 CCTGCGCAGC GGCCTGGGGG GCGCCCCCCT GGGGCTGCGG CTGCCCCGGG AGACCGACGA
            361 AGAGCCCGAG GAGCCCGGCC GGAGGGGCAG CTTTGTGGAG ATGGTGGACA ACCTGAGGGG
            421 CAAGTCGGGG CAGGGCTACT ACGTGGAGAT GACCGTGGGC AGCCCCCCGC AGACGCTCAA
            481 CATCCTGGTG GATACAGGCA GCAGTAACTT TGCAGTGGGT GCTGCCCCCC ACCCCTTCCT
            541 GCATCGCTAC TACCAGAGGC AGCTGTCCAG CACATACCGG GACCTCCGGA AGGGTGTGTA
            601 TGTGCCCTAC ACCCAGGGCA AGTGGGAAGG GGAGCTGGGC ACCGACCTGG TAAGCATCCC
            661 CCATGGCCCC AACGTCACTG TGCGTGCCAA CATTGCTGCC ATCACTGAAT CAGACAAGTT
            721 CTTCATCAAC GGCTCCAACT GGGAAGGCAT CCTGGGGCTG GCCTATGCTG AGATTGCCAG
            781 GCCTGACGAC TCCCTGGAGC CTTTCTTTGA CTCTCTGGTA AAGCAGACCC ACGTTCCCAA
            841 CCTCTTCTCC CTGCAGCTTT GTGGTGCTGG CTTCCCCCTC AACCAGTCTG AAGTGCTGGC
            901 CTCTGTCGGA GGGAGCATGA TCATTGGAGG TATCGACCAC TCGCTGTACA CAGGCAGTCT
            961 CTGGTATACA CCCATCCGGG CGGGAGTGGT ATTATGAGGT GATCATTTGT GCGGTGGGAG
           1021 ATCAATGGAC AGGATCTGAA AATGGACTGC AGAGTACACT ATGACAGGAG CAATGTGGAC
           1081 AGTGGCAACC AACCACTTTC GGTTTGGCTC AGAAAGTGTT TGAAGCTGCA GTTCCAATTC
          1141 ATCAGCAGTC CTCATCCGGG GGA
          //
          Primer: BGH_rev2
          Sequence file: RDB14343_A6Itb.seq check
          >D03881A1_A6It_1_BGH_rev2_B11_02_ABI08.ab1
              1 CCATTCAGGG GATAGGAGCA CGGGGGAGGG GCAAACAACA GATGGCTGGC AACTAGAAGG
             61 CACAGTCGAG GCTGATCAGC GGGTTTAAAC TCAATGGTGA TGGTGATGAT GACCGGTATG
            121 CATATTCAGA TCCTCTTCTG AGATGAGTTT TTGTTCGAAG GGCCCTCTAG ACTCGAGTGA
            181 CTCATCTGTC TGTGGAATGT TGTAGCCACA GTCTTCCATG TCCAAGGTGA CAAAAGGGCC
            241 TTCCACCGCT GCCGTCCTGA ACTCATCGTG CACATGGCAA GCGCTGACAG CAAAGCCAAT
            301 TCGTTTTCGG GCCCGATCAA AGACAACGTA GAAGCCCTCC ATGATAACAG CTCCCATAAC
            361 AGTGCCCGTG GATGACTGTG AGATGGCAAA CTTGTAACAG TCGTCTTGGG ACGTGGCCAC
            421 ATCTTCCACT GGCCGCAGGT ATTGCTGCGG AAGGATGGTG ATGCGGAAGG ACTGGTTGGT
            481 AACCTCACCC ATTAGGTAGA GTGAGATGAC TGGGAAAATG TTCCAAGGGG TGGTGCCTGC
            541 TTGCCAGCAC ACCAGCTGCT CTCCTAGCCA GAAACCATCA GGGAACTTCT CCGTGGAGGA
            601 GGCTGCCTTG ATGGATTTGA CTGCAGCTTC AAACACTTTC TTGGGCAAAC GAAGGTTGGT
            661 GGTGCCACTG TCCACAATGC TCTTGTCATA GTTGTACTCC TTGCAGTCCA TTTTCAGATC
            721 CTGTCCATTG ATCTCCACCC GCACAATGAT CACCTCATAA TACCACTCCC GCCGGATGGG
            781 TGTATACCAG AGACTGCCTG TGTACAGCGA GTGGTCGATA CCTCCAATGA TCATGCTCCC
            841 TCCGACAGAG GCCAGCACTT CAGACTGGTT GAGGGGGAAG CCAGCACCAC AAAGCTGCAG
            901 GGAGAAGAGG TTGGGAACGT GGGTCTGCTT TACCAGAGAG TCAAAGAAAG GCTCCAGGGA
            961 GTCGTCAGGC CTGGCAATCT CAGCATAGGC CAGCCCAGGA TGCCTTCCCA GTTGGAGCCG
           1021 TTGATGAAAG AACTTGTCTG ATTCAGTGAT GGCAGCAATG TGGCACGCAC AGTGACGTTG
           1081 GGGGTCATGG GGGGATGCTA ACCAGTCGTC CTAGCTCCCA TCCAACTGGC CCTGGGTGTA
          1141 GGCCACAATA CACACCCCTT CCGGA
          //
          Primer: SV40pro_F
          Sequence file: RDB14343_A6Itc.seq check
          >D03881A1_A6It-1_SV40pro_F_E12_05_ABI08.ab1
              1 GCCTTGTCCT GGGGCAATTC AGAAGTAGTG AGGAGGCTTT TTTGGAAGGC CTAGGCTTTT
             61 GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAGCAC GTGTTGACAA
            121 TTAATCATCG GCATAGTATA TCGGCATAGT ATAATACGAC AAGGTGAGGA ACTAAACCAT
            181 GGCCAAGCCT TTGTCTCAAG AAGAATCCAC CCTCATTGAA AGAGCAACGG CTACAATCAA
            241 CAGCATCCCC ATCTCTGAAG ACTACAGCGT CGCCAGCGCA GCTCTCTCTA GCGACGGCCG
            301 CATCTTCACT GGTGTCAATG TATATCATTT TACTGGGGGA CCTTGTGCAG AACTCGTGGT
            361 GCTGGGCACT GCTGCTGCTG CGGCAGCTGG CAACCTGACT TGTATCGTCG CGATCGGAAA
            421 TGAGAACAGG GGCATCTTGA GCCCCTGCGG ACGGTGCCGA CAGGTGCTTC TCGATCTGCA
            481 TCCTGGGATC AAAGCCATAG TGAAGGACAG TGATGGACAG CCGACGGCAG TTGGGATTCG
            541 TGAATTGCTG CCCTCTGGTT ATGTGTGGGA GGGCTAAGCA CTTCGTGGCC GAGGAGCAGG
            601 ACTGACACGT GCTACGAGAT TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG
            661 GAATCGTTTT CCGGGACGCC GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT
            721 TCTTCGCCCA CCCCAACTTG TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA
            781 TCACAAATTT CACAAATAAA GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC
            841 TCATCAATGT ATCTTATCAT GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT
            901 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC
            961 GAGCCGGAAG CATAAAGTGT AAAGCCTGGG GGTGCCTAAT GAGTGAGCTA ACTCACATTA
           1021 ATTGCGTTGC GCTCACTGCC CCGCTTTCCC AGTCGGGAAC CCTGTCGTGC CAGCTGCATT
           1081 AAATGAATCG GGCACGGCGC GGGAGAGGCG GGTTGCGTAA TGGGCGCCTC TCCGCATCCT
          1141 CGCCTCACTG AACTCGGCGT GGCGCGCTCC TCCGGGATTC GCGATTTTG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kizuka, Y., Bisecting GlcNAc modification stabilizes BACE1 protein under oxidative stress conditions. Biochem. J. 473 (1): 21-30 (2016) PMID 26467158.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl