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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pPAL7_GimRET

Expression vector of GimRET (Glycine inserted mutant FRET probe) for E. coli.

Catalog number RDB14203
Resource name pPAL7_GimRET
Alternative name CFP-YFP1G GimRET
Clone info. Expression vector of glycine-inserted mutant FRET (GimRET) fusion protein for E.coli. GimRET works as a protein-crowding sensor that consists of CFP with YFP1G (one glycine insertion).
Comment YFP1G (Inserted a Gly residue in the position of the 145th Tyr residue).
Vector backbone pAL7 (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Watanabe, Tomonobu M. |
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External Database
Aequorea victoria GFP

            Reference sequence
              
             

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Morikawa, T.J. et al., Sci. Rep. 6: 22342, 2016)
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Morikawa, T.J. et al., Sci. Rep. 6: 22342, 2016)。
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            RDB14203 pPAL7_GimRET DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pPAL7_GimRET was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14203).

            Reference section:

            Konishi, H.A., Asai, S., Watanabe, T.M., Yoshimura, S.H., In vivo analysis of protein crowding within the nuclear pore complex in interphase and mitosis. Sci. Rep. 7 (1): 5709 (2017). PMID 28720791.
            Morikawa, T.J., Fujita, H., Kitamura, A., Horio, T., Yamamoto, J., Kinjo, M., Sasaki, A., Machiyama, H., Yoshizawa, K., Ichimura, T., Imada, K., Nagai, T., Watanabe, T.M., Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it. Sci. Rep. 6: 22342 (2016). PMID 26956628.

            Further references such as user reports and related articles (go to bottom)


            References and tips

            Featured content

            Featured content Dnaconda's recommendation EXR0059e (English text)
            Featured content Dnaconda's recommendation EXR0059j (Japanese text)
            Featured content Sensor and Visualization (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14203_A6Fop1-2.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: pBR322_BamHI_R (Pr0065)
            Region: lac O,RBS,Profinity eXact tag,ECFP 5'
            Sequence file: RDB14203_A6Fod.seq check
            >D03467A1_A6Fo_1_pBR322BamHI_R_G02_20.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTNG GGNNNNNNTG AGCGGNNAAC AATTCCCCTC
               61 TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACAT ATGGGAGGGA AATCAAACGG
              121 GGAAAAGAAA TATATTGTCG GGTTCAAACA GGGCTTTAAG AGCTGCGCTA AGAAGGAGGA
              181 TGTCATTTCT GAAAAAGGCG GGAAACTCCA AAAGTGCTTC AAATATGTAG ACGCAGCTAG
              241 CGCTACATTA AACGAAAAAG CTGTAGAAGA ATTGAAAAAA GATCCGAGCG TCGCGTACGT
              301 AGAAGAAGAC AAGCTCTTCA AAGCTTTGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT
              361 GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG
              421 CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG
              481 CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCCTG ACCTGGGGCG TGCAGTGCTT
              541 CAGCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG
              601 CTACGTCCAG GANCGCACCA TCTTCTTCAA GGACGACNGC AACTACNAGA NCCGCGCCGA
              661 NGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAANGGCA TCGACTTCAN
              721 NGANGANGGC AACATCCTGG GGCACNANCT GNANTANANC TACNTCAGCN NNNACGTCTA
              781 TATCNCCGCC GANNAGCNNA ANANNGGCAT NNAGGCNNNN TTCNNNATNC NNCCACNNNN
              841 TCNNNGANNG NANNNNNGCA NNTNNNNNAN NNNTNCNNNN NNANNNNCCC CNNTCGGCGN
            901 NGNNCCNNNN NNNNNN
            //
            Primer: T7ter_R1 (Pr0798)
            Region: EYFP 3'(YFP1G)
            Sequence file: RDB14203_A6Foe.seq check
            >14203_14203_B1H2F_T7ter_R1_A03_03_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNTTGCTN NGCGGNGGCA GCNNGCCAAC TCAGCTTCCT
               61 TTCGGGCTTA TGCGGCCGCC TCGAGTTACT TGTACAGCTC GTCCATGCCG AGAGTGATCC
              121 CGGCGGCGGT CACGAACTCC AGCAGGACCA TGTGATCGCG CTTCTCGTTG GGGTCTTTGC
              181 TCAGGGCGGA CTGGTAGCTC AGGTAGTGGT TGTCGGGCAG CAGCACGGGG CCGTCGCCGA
              241 TGGGGGTGTT CTGCTGGTAG TGGTCGGCGA GCTGCACGCT GCCGTCCTCG ATGTTGTGGC
              301 GGATCTTGAA GTTCACCTTG ATGCCGTTCT TCTGCTTGTC GGCCATGATA TAGACGTTGT
              361 GGCTGTTGTA TCCGTTGTAC TCCAGCTTGT GCCCCAGGAT GTTGCCGTCC TCCTTGAAGT
              421 CGATGCCCTT CAGCTCGATG CGGTTCACCA GGGTGTCGCC CTCGAACTTC ACCTCGGCGC
              481 GGGTCTTGTA GTTGCCGTCG TCCTTGAAGA AGATGGTGCG CTCCTGGACG TAGCCTTCGG
              541 GCATGGCGGA CTTGAAGAAG TCGTGCTGCT TCATGTGGTC GGGGTAGCGG GCGAAGCACT
              601 GCANGCCGTA NCCGAANGTG GTCACGAGGG TGGGCCAGGG NACGGGCAGC TTGNCGGTGG
              661 TGCANANGNA NNTCNNNNNC AGCTNGNCGT NNNGGNATCN NCCNCNNCNN CNNNNGNANA
            721 CNCNNAANTT NNGGNNNNTN NNNNNNNNCN NNNNN
            //
            Primer: EYFP-H_R (Pr1036)
            Region: ECFP mid
            Sequence file: RDB14203_A6Fof.seq check
            >14203_14203_B1H2F_EYFP-H_R_B03_06_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNNNGCTGG NGCTGCCTCC GGNCTTGTAC NGCTCGTCCA
               61 TGCCGAGAGT GATCCCGGCG GCGGTCACGA ACTCCAGCAG GACCATGTGA TCGCGCTTCT
              121 CGTTGGGGTC TTTGCTCAGG GCGGACTGGG TGCTCAGGTA GTGGTTGTCG GGCAGCAGCA
              181 CGGGGCCGTC GCCGATGGGG GTGTTCTGCT GGTAGTGGTC GGCGAGCTGC ACGCTGCCGT
              241 CCTCGATGTT GTGGCGGATC TTGAAGTTGG CCTTGATGCC GTTCTTCTGC TTGTCGGCGG
              301 TGATATAGAC GTTGTGGCTG ATGTAGTTGT ACTCCAGCTT GTGCCCCAGG ATGTTGCCGT
              361 CCTCCTTGAA GTCGATGCCC TTCAGCTCGA TGCGGTTCAC CAGGGTGTCG CCCTCGAACT
              421 TCACCTCGGC GCGGGTCTTG TAGTTGCCGT CGTCCTTGAA GAAGATGGTG CGCTCCTGGA
              481 CGTAGCCTTC GGGCATGGCG GACTTGAAGA AGTCGTGCTG CTTCATGTGG TCGGGGTAGC
              541 GGCTGAAGCA CTGCACGCCC CAGGTCANGG TGGTCACGAN GGTGGGCCAG GGCACGGGCA
              601 GCTTGCCGGT GGTGCAGATG AACTTCAGGG TCNNCTTGCC GNNNNGGCAT CGCCCTCNCC
            661 CTCNNCNGAC NNNCTNNNNN NNNNN
            //
            Primer: ECFP-C_F1 (Pr0925)
            Region: ECFP 3',EYFP(YFP1G) 5'
            Sequence file: RDB14203_A6Fog.seq check
            >14203_14203_B1H2F_ECFP-C_F1_C03_09_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNNCNNNNNN CNTGGTCCTG CTGGAGTTCG TGACCGCCGC
               61 CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGTCCGGA GGCAGCACCA GCGGTACTGG
              121 AGGTTCAGGC GGCTCAGGTG GAACTGGTGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT
              181 GGTGCCCATC CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG
              241 CGAGGGCGAG GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG
              301 CAAGCTGCCC GTGCCCTGGC CCACCCTCGT GACCACCTTC GGCTACGGCC TGCAGTGCTT
              361 CGCCCGCTAC CCCGACCACA TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG
              421 CTACGTCCAG GAGCGCACCA TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA
              481 GGTGAAGTTC GAGGGCGACA CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA
              541 GGAGGACGGC AACATCCTGG GGCACAAGCT GGAGTACAAC GGATACAACA GCCACNACGT
              601 CTATATCATG GCCGACAAGC AGAANAACNG CATCNAGGTG AACTTCNAGA NNCGCCNCNA
              661 CATCGAGGAN NGCAGNNTGC ANCTCNNNGA NCNCTACCAG CAGANNACCC CNTCNGNCGA
            721 NNNNCNNNNN NNNNN
            //
            Primer: lacIpro_F3 (Pr0722)
            Region: lacI pro,lacI
            Sequence file: RDB14203_A6Foh.seq check
            >14203_14203_B1H2F_laclpro_F3_D03_12_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNCTGGGCTG CTTCCTAATG CAGGAGTCGC ATAAGGGAGA
               61 GCGTCGAGAT CCCGGACACC ATCGAATGGC GCAAAACCTT TCGCGGTATG GCATGATAGC
              121 GCCCGGAAGA GAGTCAATTC AGGGTGGTGA ATGTGAAACC AGTAACGTTA TACGATGTCG
              181 CAGAGTATGC CGGTGTCTCT TATCAGACCG TTTCCCGCGT GGTGAACCAG GCCAGCCACG
              241 TTTCTGCGAA AACGCGGGAA AAAGTGGAAG CGGCGATGGC GGAGCTGAAT TACATTCCCA
              301 ACCGCGTGGC ACAACAACTG GCGGGCAAAC AGTCGTTGCT GATTGGCGTT GCCACCTCCA
              361 GTCTGGCCCT GCACGCGCCG TCGCAAATTG TCGCGGCGAT TAAATCTCGC GCCGATCAAC
              421 TGGGTGCCAG CGTGGTGGTG TCGATGGTAG AACGAAGCGG CGTCGAAGCC TGTAAAGCGG
              481 CGGTGCACAA TCTTCTCGCG CAACGCGTCA GTGGGCTGAT CATTAACTAT CCGCTGGATG
              541 ACCAGGATGC CATTGCTGTG GAAGCTGCCT GCACTAATGT TCCGGCGTTA TTTCTTGATG
              601 TCTCTGACCA GACACCCATC AACAGTATTA TTTTCTCCCN TGAANANGGN ACGCGACTGG
              661 GCGNNNNNCA TCTGNTCGCA TTGGGTCANC AGCAANTCGN GCTNNNNNCG GGNCNNNNNN
            721 NNNN
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Konishi, H.A., In vivo analysis of protein crowding within the nuclear pore complex in interphase and mitosis. Sci. Rep. 7 (1): 5709 (2017). PMID 28720791.
            original Morikawa, T.J., Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it. Sci. Rep. 6: 22342 (2016). PMID 26956628.
            reference Machiyama, H., The use of a genetically encoded molecular crowding sensor in various biological phenomena. Biophys. Physicobiol. 14: 119-125 (2017). PMID 28900589.
            review Watanabe, T.M., An intracellular crowding sensor based on fluorescent protein. (Japanese text) Journal of Japanese Biochemical Society 89 (4): 577-586 (2017).

            2023.05.01

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