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pGL3_hBmalPro-Luc(-829_+57 RORE1 mut/RORE2 mut)

Reporter plasmid of E-box (E1 and E2 mutants) of human Baml 1 promoter.

Catalog number RDB14182
Resource name pGL3_hBmalPro-Luc(-829_+57 RORE1 mut/RORE2 mut)
Clone info. Expression vector of SV40 promoter driving a firefly luciferase with E-box (-829, E1 mut/E2 mut) of human Baml 1 promoter (+1 is the putative transcription start site).
Comment Expression was confirmed by the depositor with culture-based luminescent repoter assay.
Vector backbone pGL3-Basic vector (Plasmid)
Size of vector backbone 4.8 kb
Selectable markers Amp^r
Gene/insert name human Bmal 1 genomic
Depositor|Developer Takumi, Toru |
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External Database
human Bmal 1

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akashi, M. et al., Nat. Struct. Mol. Biol. 12(5):441-448, 2005)。
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          RDB14182 pGL3_hBmalPro-Luc(-829_+57 RORE1 mut/RORE2 mut) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Akashi, M., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Circadian clock (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14182_A6F3p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Region: insert 5'
          Sequence file: RDB14182_A6F3a.seq check
          >D03420B5_A6F3_1_pGL4-4174F_G04_19.ab1
              1 NNNNNNNNNN NNTTNNNNNN TCGNNANGTN NCGNNCTCTT ACGCGTGCTA GCCCGGGCTC
             61 GAGTATTCTC TTTGGAACTA CGGGAATGAC AGCTCCAGTG GGACACTTGG AGGTCATGAT
            121 GAACGCAAAG AACGTGAGAA CACTTGCAGT CCTCGGGGTG GAAATGCCTT CTAGAAATAC
            181 TAAGTATCCG TTCTCTCGTT GAGACTTTGG CAAACCAGGG ATTTTAGGAA GGGTCTGGCA
            241 CTCAACGTTC CTAAAATTGG TTTCCTAGAT GGAGCCGGAG GGAGAGAGGG AGTCAGGAAC
            301 TGCTGCTTTT CGTCGGAATG CCCGGAGGGG AGAAGAAGAA GGAGGAGGGG TGAGGGCGGG
            361 CGGCCAAACG CCAGCCGGCA GGGGACCCAG AGAAGAGGGA CATCCCGGGC GACCCCGAGG
            421 AGCGCGGCTT GGGCACCGCA GTGGCCGCGG CTAGTGGGAG ACCTGAGGGG AAAGGGAGAG
            481 GGCAGGGGCG AGGAACCCAG GGAGCGCGCG CGGATTGGTC CTCTCCTCGG GGCGTGCGCT
            541 CCTGTGCGCC AAATGATTGG TGGCAGGAAA GTAGCAGGTA AACCGGCTCC CGCCGCCTCG
            601 CCATTGGTCA GCGGCCTCTC TAGCCGCCAC ACAACTGGCC AGCGGGCTGC CGAGCCGCGC
            661 GCGGATTGGC TGGGGGCGGC CGCCGGGACC GGCTCCCTTC GGGCGTTCGG ATTGGCTGGC
            721 GGGAANANGC ANGTATCCGG GCGCTGCGGC TCCTCCATTG GTGGGCGGGG AANGGGGGTT
            781 GGGCACAGCG ATTGGTNGNC GGGGGGGCCG GGCCTGGGCC GGCGGGGNNC GGNNTGGNCN
            841 GAAANTGCTA NCGTGGNNCG ACATTTNNNG NANNNNANAA NNNNNCNANG GNNNNNNNNN
          901 N
          //
          Primer: Luc_R
          Region: Luc 5',insert 3'
          Sequence file: RDB14182_A6F3b.seq check
          >D03420B5_A6F3_1_Luc_R_H04_22.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNCTCTT NNTAGCCTTA TGCAGTTGCT CTCCNGCGGT
             61 TCCATCTTCC AGCGGNTAGA ATGGCGCCGG GCCTTTCTTT ATGTTTTTGG CGTCTTCCAT
            121 GGTGGCTTTA CCAACAGTAC CGGAATGCCA AGCTTACTTA GATCGCAGAT CCCGCGGCGG
            181 CGGCGGCGGT GAGTCCGGCG CGGGTAAACA GGCACCTCCG TCCCCTCGAG ACTTTCTGCC
            241 TTCCCTAAAT GTCGCACCAC GCTAGCACTT TCCGACCAAT CCGCTCCCCG CCGGCCCAGG
            301 CCCGGCCCCC CGCCCACCAA TCGCTGTGCC CAACCCCCCT TCCCCGCCCA CCAATGGAGG
            361 AGCCGCAGCG CCCGGATACC TGCCTCTTCC CGCCAGCCAA TCCGAACGCC CGAAGGGAGC
            421 CGGTCCCGGC GGCCGCCCCC AGCCAATCCG CGCGCGGCTC GGCAGCCCGC TGGCCAGTTG
            481 TGTGGCGGCT AGAGAGGCCG CTGACCAATG GCGAGGCGGC GGGAGCCGGT TTACCTGCTA
            541 CTTTCCTGCC ACCAATCATT TGGCGCACAG GAGCGCACGC CCCGAGGAGA GGACCAATCC
            601 GCGCGCGCTC CCTGGGTTCC TCGCCCCTGC CCTCTCCCTT TCCCCTCANG TCTCCCACTA
            661 GCCGCGGCCA CTGCGGTGCC CAAGCCGCGC TCCTCGGGGT CGCCCGGGAN GTCCCTCTTC
            721 TCTGGGTCCC CTGCCGGCTG GCGTTTGGCC GCCCGCCCTC ACCCCTCCTC CTTCTTCTTC
            781 TCCCCTCCGG GCATTCCGAC NAAAAGCAGC AGNNCCTGAC TCCCTCTCTC CCTCCGGCTN
            841 CATCTANGAA ANNNATTTTA NGAACGTTNN NNNNCAGANC CNNCCNNAAN TCCNNNGNNT
          901 NNCCAANNTC TCAACNANNN NNNNGNNNNN TNNNNNTNCN NNNNNNNNN
          //
          Primer: SV40pA_R
          Region: SV40pA,Luc 3'
          Sequence file: RDB14182_A6F3c.seq check
          >14182_14182_B0J6F_SV40pA_R_H06_24_ABI24.ab1
              1 NNNNNNNNNN NCNCNNATTT NNNNAATAAA GCATTTTTTT NNCTGCATTC TAGTTGTGGT
             61 TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGCTCGA AGCGGCCGGC CGCCCCGACT
            121 CTAGAATTAC ACGGCGATCT TTCCGCCCTT CTTGGCCTTT ATGAGGATCT CTCTGATTTT
            181 TCTTGCGTCG AGTTTTCCGG TAAGACCTTT CGGTACTTCG TCCACAAACA CAACTCCTCC
            241 GCGCAACTTT TTCGCGGTTG TTACTTGACT GGCGACGTAA TCCACGATCT CTTTTTCCGT
            301 CATCGTCTTT CCGTGCTCCA AAACAACAAC GGCGGCGGGA AGTTCACCGG CGTCATCGTC
            361 GGGAAGACCT GCGACACCTG CGTCGAAGAT GTTGGGGTGT TGGAGCAAGA TGGATTCCAA
            421 TTCAGCGGGA GCCACCTGAT AGCCTTTGTA CTTAATCAGA GACTTCAGGC GGTCAACGAT
            481 GAAGAAGTGT TCGTCTTCGT CCCAGTAAGC TATGTCTCCA GAATGTAGCC ATCCATCCTT
            541 GTCAATCAAG GCGTTGGTCG CTTCCGGATT GTTTACATAA CCGGACATAA TCATAGGACC
            601 TCTCACACAC AGTTCGCCTC TTTGATTAAC GCCCAGCGTT TTCCCGGTAT CCAGATCCAC
            661 AACCTTCGCT TCAAAAAATG GAACAACTTT ACCGACCGCG CCCGGTTTAT CATCCCCCTC
            721 GGGTGTAATC AGAATAGCTG ATGTAGTCTC AGTGAGCCCA TATCCTTGCC TGATACCTGG
            781 CAGATGGAAC CTCTTGGCAA CCGCTTCCCC GACTTCCTTA GAGAGGGGAG CGCCACCAGA
            841 AGCAATTTCG TGTAAATTAN ATAAATCGNA TTTGTCAATC ANANNGCTTT TGGCGAANAA
            901 NGANAATANG GTTGGCACCN GCAGCGCACT TTGAATCNTG NAANCCNGNA NNNNTNNNCA
          961 NAANNAGCNC NTCNTNNNNN NNTACNNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akashi, M., The orphan nuclear receptor RORalpha regulates circadian transcription of the mammalian core-clock Bmal1. Nat. Struct. Mol. Biol. 12 (5): 441-448 (2005). PMID 15821743.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl