Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMV-TUBA1A myc-C25F

Expression vector of Myc-tagged human TUBA1A mutant, C64F.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14146_A6Dpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB14146_A6Dpa.seq
>D03180A7_A6Dp_1_CMV-Forward_A11_02.ab1     1 ATTAACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC    61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGATTAC GCCAAGCTCG AAATTAACCC   121 TCACTAAAGG GAACAAAAGC TGGAGCTCCA CCGCGGTGGC GGCCGCCATG CGTGAGTGCA   181 TCTCCATCCA CGTTGGCCAG GCTGGTGTCC AGATTGGCAA TGCCTGCTGG GAGCTCTACT   241 TCCTGGAACA CGGCATCCAG CCCGATGGCC AGATGCCAAG TGACAAGACC ATTGGGGGAG   301 GAGATGATTC CTTCAACACC TTCTTCAGTG AGACGGGGGC TGGCAAGCAT GTGCCCCGGG   361 CAGTGTTTGT AGACTTGGAA CCCACAGTCA TTGATGAAGT TCGCACTGGC ACCTACCGCC   421 AGCTCTTCCA CCCTGAGCAA CTTATCACAG GCAAGGAAGA TGCTGCCAAT AACTATGCCC   481 GAGGGCACTA CACCATTGGC AAGGAGATCA TTGACCTCGT GTTGGACCGA ATTCGCAAGC   541 TGGCCGACCA GTGCACGGGT CTCCAGGGCT TCTTGGTTTT CCACAGCTTT GGTGGGGGAA   601 CTGGTTCTGG GTTCACCTCC CTGCTCATGG AACGTCTCTC AGTTGATTAT GGCAAGAAGT   661 CCAAGCTGGA GTTCTCTATT TACCCGGCGC CCCAGGTTTC CACAGCTGTA GTTGAGCCCT   721 ACAACTCCAT CCTCACCACC CACACCACCC TGGAGCACTC TGATTGTGCC TTCATGGTAG   781 ACAATGAGGC CATCTATGAC ATCTGTCGTA GAAACCTCGA TATTGAGCGT CCAACCTATA   841 CTAACCTGAA TAGGTTAATA GGTCAAATTG TGTCCTCCAT CACTGCTTCC CTGAGATTTG   901 ATGGAGCCCT GAAATGTTGA CCCTGACAGA ATTTCCAGAC CAAACCCTGG TGCCTATCCC   961 CGCATCCACT TTCCCTCTGG CACATATGCC CCTGTCATCT CTGCCTGAGA AAGTCTACCC  1021 ATGGAACCAG CCTTTCCTGG TAAGCAGAGA TCGCGAAGTG CTGCCGTTGA AGTCAGCCGA 1081 AACCAGAATG //
Primer: T7_20bp Sequence file: RDB14146_A6Dpb.seq
>D03180A7_A6Dp_1_T7_20bp_B11_05.ab1     1 TATTACTGGT ACTTATTATT AAGGTACCGG GCCCTACAGA TCCTCTTCTG AGATGAGTTT    61 TTGTTCCTCG AGGTCGACGG TATCGATAAG CTTGATATCG AATTCCTGCA GGTATTCCTC   121 TCCTTCTTCC TCACCCTCTC CTTCAACAGA ATCCACACCA ACCTCCTCAT AATCCTTCTC   181 AAGGGCAGCC ATGTCCTCAC GGGCCTCTGA AAACTCACCT TCCTCCATCC CCTCCCCAAC   241 GTACCAGTGA ACAAAGGCAC GTTTGGCATA CATCAGGTCA AACTTGTGGT CCAGGCGAGC   301 CCAGGCCTCA GCAATGGCTG TGGTGTTGCT CAGCATGCAC ACAGCTCTCT GTACCTTGGC   361 CAGGTCTCCA CCAGGCACCA CAGTGGGAGG CTGGTAGTTG ATGCCAACCT TGAAGCCAGT   421 GGGGCACCAA TCCACAAACT GGATGGTACG CTTGGTCTTG ATGGTGGCAA TGGCAGCATT   481 GACATCTTTG GGAACCACGT CACCACGGTA CAACAGGCAG CAAGCCATGT ATTTACCATG   541 GCGAGGGTCA CATTTCACCA TCTGGTTGGC TGGCTCAAAG CAAGCATTGG TGATCTCTGC   601 TACAGAAAGC TGTTCATGGT AGGCTTTCTC AGCAGAGATG ACAGGGGCAT ATGTGGCCAG   661 AGGGAAGTGG ATGCGGGGAT AGGGCACCAG GTTGGTCTGG AATTCTGTCA GGTCAACATT   721 CAGGGCTCCA TCAAATCTCA GGGAAGCAGT GATGGAGGAC ACAATTTGAC CTATTAACCT   781 ATTCAGGTTA GTATAGGTTG GACGCTCAAT ATCGAGGTTT CTACGACAGA TGTCATAGAT   841 GGCCTCATTG TCTACCATGA AAGGCACAAT CAGAGTGCTC CAGGGTGGTG TGGTGTGAGA   901 TGGGGGGAAG TTTGTGTAGG CTTCAACCTA ACAGGCCTGT GGAAAACCTG GGGGCGCCCG   961 GGGTAAATAG AAGAAACTCC CAGCTTTGGT ACTTCTTTGT CATTATTCCA CCTGAGAGGA 1021 CGGATGCAAT GAAGCCAAGG AGGATGCACT CAGATCAGCT TCCCCAACCA AAGTCT //
Primer: SV40pro_F_V2 Sequence file: RDB14146_A6Dpc.seq
>D03180A7_A6Dp_1_SV40pro_F_V2_C11_08.ab1     1 GGGGGCTTTT TGGAGCCTAG GCTTTTGCAA GATCGATCAA GAGACAGGAT GAGGATCGTT    61 TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG TGGAGAGGCT   121 ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG TGTTCCGGCT   181 GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG CCCTGAATGA   241 ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC CTTGCGCAGC   301 TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG AAGTGCCGGG   361 GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA TGGCTGATGC   421 AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC AAGCGAAACA   481 TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG ATGATCTGGA   541 CGAAGAACAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG CGAGCATGCC   601 CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA TCATGGTGGA   661 AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG ACCGCTATCA   721 GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAACTT GGCGGCGAAT GGGCTGACCG   781 CTTCCTCGTG CTTTACGGTA TCGCCGCTCC CGATTCGCAG CGCATCGCCT TCTATCGCCT   841 TCTTGACGAG TTCTTCTGAG CGGGACTCTG GGGGTTCGAA ATGACCGACC AAGCGACGCC   901 CAACCTGCCA TCACGAGATT TCGATTCCAC CGCCGCCCTT CTATGAAAGG TTGGGCTTCG   961 GAATCGTTTT CCGGGACGCC CGGCTGGGAT GAATCTCAGC GCGGGGATCT CATGCCTGAA 1021 GTCCTCCGCC GATCCTTAGG GAAGCTTAAC CTGAACACG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl