Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMV-TUBA1A flag-C25F

Expression vector of FLAG-tagged human TUBA1A mutant, C25F.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14142_A6Dpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB14142_A6Dpa.seq
>D03180A3_A6Dp_3_CMV-Forward_C06_09.ab1     1 CGTTACTCAC CATTGACGCA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA    61 GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGATTACG CCAAGCTCGA AATTAACCCT   121 CACTAAAGGG AACAAAAGCT GGAGCTCCAC CGCGGTGGCG GCCGCCATGC GTGAGTGCAT   181 CTCCATCCAC GTTGGCCAGG CTGGTGTCCA GATTGGCAAT GCCTGCTGGG AGCTCTACTT   241 CCTGGAACAC GGCATCCAGC CCGATGGCCA GATGCCAAGT GACAAGACCA TTGGGGGAGG   301 AGATGATTCC TTCAACACCT TCTTCAGTGA GACGGGGGCT GGCAAGCATG TGCCCCGGGC   361 AGTGTTTGTA GACTTGGAAC CCACAGTCAT TGATGAAGTT CGCACTGGCA CCTACCGCCA   421 GCTCTTCCAC CCTGAGCAAC TTATCACAGG CAAGGAAGAT GCTGCCAATA ACTATGCCCG   481 AGGGCACTAC ACCATTGGCA AGGAGATCAT TGACCTCGTG TTGGACCGAA TTCGCAAGCT   541 GGCCGACCAG TGCACGGGTC TCCAGGGCTT CTTGGTTTTC CACAGCTTTG GTGGGGGAAC   601 TGGTTCTGGG TTCACCTCCC TGCTCATGGA ACGTCTCTCA GTTGATTATG GCAAGAAGTC   661 CAAGCTGGAG TTCTCTATTT ACCCGGCGCC CCAGGTTTCC ACAGCTGTAG TTGAGCCCTA   721 CAACTCCATC CTCACCACCC ACACCACCCT GGAGCACTCT GATTGTGCCT TCATGGTAGA   781 CAATGAGGCC ATCTATGACA TCTGTCGTAG AAACCTCGAT ATTGAGCGTC CAACCTATAC   841 TAACCTGAAT AGGTTAATAG GTCAAATTGT GTCCTCCATC ACTGCTTCCC TGAGATTTGA   901 TGGAGCCCTG AAATGTTGAC CTGACAGAAA TTCCAGACCA AACCTGGTGC CCTATCCCCC   961 GCATCCCACT TCCCTCTGGC CACATATGCC CCCTGTCATC TCTGCTGGAG AAAGGCCTAC  1021 CATGAACCAG GCTTTTCCTG GTAGCCAGAG ATCACGAATG CCATGCCATT GAAGCCAAGC 1081 CAACCAGAAA TTGGATTGA //
Primer: T7_20bp Sequence file: RDB14142_A6Dpb.seq
>D03180A3_A6Dp_3_T7_20bp_G06_21.ab1     1 TATTACTGGT ACTTATTATT AAGGTACCGG GCCCTACTTA TCGTCGTCAT CCTTGTAATC    61 CTCGAGGTCG ACGGTATCGA TAAGCTTGAT ATCGAATTCC TGCAGGTATT CCTCTCCTTC   121 TTCCTCACCC TCTCCTTCAA CAGAATCCAC ACCAACCTCC TCATAATCCT TCTCAAGGGC   181 AGCCATGTCC TCACGGGCCT CTGAAAACTC ACCTTCCTCC ATCCCCTCCC CAACGTACCA   241 GTGAACAAAG GCACGTTTGG CATACATCAG GTCAAACTTG TGGTCCAGGC GAGCCCAGGC   301 CTCAGCAATG GCTGTGGTGT TGCTCAGCAT GCACACAGCT CTCTGTACCT TGGCCAGGTC   361 TCCACCAGGC ACCACAGTGG GAGGCTGGTA GTTGATGCCA ACCTTGAAGC CAGTGGGGCA   421 CCAATCCACA AACTGGATGG TACGCTTGGT CTTGATGGTG GCAATGGCAG CATTGACATC   481 TTTGGGAACC ACGTCACCAC GGTACAACAG GCAGCAAGCC ATGTATTTAC CATGGCGAGG   541 GTCACATTTC ACCATCTGGT TGGCTGGCTC AAAGCAAGCA TTGGTGATCT CTGCTACAGA   601 AAGCTGTTCA TGGTAGGCTT TCTCAGCAGA GATGACAGGG GCATATGTGG CCAGAGGGAA   661 GTGGATGCGG GGATAGGGCA CCAGGTTGGT CTGGAATTCT GTCAGGTCAA CATTCAGGGC   721 TCCATCAAAT CTCAGGGAAG CAGTGATGGA GGACACAATT TGACCTATTA ACCTATTCAG   781 GTTAGTATAG GTTGGACGCT CAATATCGAG GTTTCTACGA CAGATGTCAT AGATGGCCTC   841 ATTGTCTACC ATGAAGGCAC AATCAGAGTG CTCCAGGGTG GTGTGGGTGG TGAAAGAAGT 901 AGATAGAATT TTGT //
Primer: SV40pro_F_V2 Sequence file: RDB14142_A6Dpc.seq
>D03180A3_A6Dp_3_SV40pro_F_V2_C08_08.ab1     1 GTGGACTTTT TTTGGAGCTA GGCTTTTGCA AGATCGATCA AGAGACAGGA TGAGGATCGT    61 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC   121 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC   181 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG   241 AACTGCAAGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG   301 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG   361 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG   421 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC   481 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG   541 ACGAAGAACA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGAGCATGC   601 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG   661 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC   721 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAACT TGGCGGCGAA TGGGCTGACC   781 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC   841 TTTCTTGACG AGTTCTTCTG AGCGGACTCT GGGGTTCGAA TGACCGACCA GCGACGCCCA   901 ACCTGCATCA CGAGATTTCG ATTTCCACCG CCGCCTTCTA TGAAGTTGGG CTTCGGAATC   961 GTTTTCGGAC GGCGCCTGGA ATGATTCTTC AGCGCGGGAA TTCTCATGGC TGGATTCTTT 1021 GGCCCACCTA GGAGCCTTAC TGACTACTCA G //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl