Please review the QC test results indicated by icon below as well as clone information before placing your order.

pCMV-TUBA1A flag-WT

Expression vector of FLAG-tagged human TUBA1A (wild type).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yokoi, S. et al., Sci. Rep. 5:15165, 2015)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB14140_A6Dpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB14140_A6Dpa.seq
>D03180A1_A6Dp_1_CMV-Forward_A04_01.ab1     1 ATTTACTCGA CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC    61 AGAGCTGGTT TAGTGAACCG TCAGATCCGC TAGCGATTAC GCCAAGCTCG AAATTAACCC   121 TCACTAAAGG GAACAAAAGC TGGAGCTCCA CCGCGGTGGC GGCCGCCATG CGTGAGTGCA   181 TCTCCATCCA CGTTGGCCAG GCTGGTGTCC AGATTGGCAA TGCCTGCTGG GAGCTCTACT   241 GCCTGGAACA CGGCATCCAG CCCGATGGCC AGATGCCAAG TGACAAGACC ATTGGGGGAG   301 GAGATGATTC CTTCAACACC TTCTTCAGTG AGACGGGGGC TGGCAAGCAT GTGCCCCGGG   361 CAGTGTTTGT AGACTTGGAA CCCACAGTCA TTGATGAAGT TCGCACTGGC ACCTACCGCC   421 AGCTCTTCCA CCCTGAGCAA CTTATCACAG GCAAGGAAGA TGCTGCCAAT AACTATGCCC   481 GAGGGCACTA CACCATTGGC AAGGAGATCA TTGACCTCGT GTTGGACCGA ATTCGCAAGC   541 TGGCCGACCA GTGCACGGGT CTCCAGGGCT TCTTGGTTTT CCACAGCTTT GGTGGGGGAA   601 CTGGTTCTGG GTTCACCTCC CTGCTCATGG AACGTCTCTC AGTTGATTAT GGCAAGAAGT   661 CCAAGCTGGA GTTCTCTATT TACCCGGCGC CCCAGGTTTC CACAGCTGTA GTTGAGCCCT   721 ACAACTCCAT CCTCACCACC CACACCACCC TGGAGCACTC TGATTGTGCC TTCATGGTAG   781 ACAATGAGGC CATCTATGAC ATCTGTCGTA GAAACCTCGA TATTGAGCGT CCAACCTATA   841 CTAACCTGAA TAGGTTAATA GGTCAAATTG TGTCCTCCAT CACTGCTTCC CTGAGATTTG   901 ATGGAGCCCT GAATGTTGAC CTGACAGAAT TTCCAGACCA ACCTGGTGCC CTATCCCCGC   961 ATCCCACTTT CCCTCTGCCC ACATATGCCC CCTGTCATCC TCCTGCCTGG AGAAAGGCCT  1021 ACCCATGGAA CAGGCTTTCT GGTAGGCAGA GGATTCACGA TGCTGGCCTT TGAGCAAGCC 1081 GAACCCAGAA ATTGCT //
Primer: T7_20bp Sequence file: RDB14140_A6Dpb.seq
>D03180A1_A6Dp_1_T7_20bp_E04_13.ab1     1 TCATTTACAG CTACTTATTA TTAAGGTACC GGGCCCTACT TATCGTCGTC ATCCTTGTAA    61 TCCTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CCTGCAGGTA TTCCTCTCCT   121 TCTTCCTCAC CCTCTCCTTC AACAGAATCC ACACCAACCT CCTCATAATC CTTCTCAAGG   181 GCAGCCATGT CCTCACGGGC CTCTGAAAAC TCACCTTCCT CCATCCCCTC CCCAACGTAC   241 CAGTGAACAA AGGCACGTTT GGCATACATC AGGTCAAACT TGTGGTCCAG GCGAGCCCAG   301 GCCTCAGCAA TGGCTGTGGT GTTGCTCAGC ATGCACACAG CTCTCTGTAC CTTGGCCAGG   361 TCTCCACCAG GCACCACAGT GGGAGGCTGG TAGTTGATGC CAACCTTGAA GCCAGTGGGG   421 CACCAATCCA CAAACTGGAT GGTACGCTTG GTCTTGATGG TGGCAATGGC AGCATTGACA   481 TCTTTGGGAA CCACGTCACC ACGGTACAAC AGGCAGCAAG CCATGTATTT ACCATGGCGA   541 GGGTCACATT TCACCATCTG GTTGGCTGGC TCAAAGCAAG CATTGGTGAT CTCTGCTACA   601 GAAAGCTGTT CATGGTAGGC TTTCTCAGCA GAGATGACAG GGGCATATGT GGCCAGAGGG   661 AAGTGGATGC GGGGATAGGG CACCAGGTTG GTCTGGAATT CTGTCAGGTC AACATTCAGG   721 GCTCCATCAA ATCTCAGGGA AGCAGTGATG GAGGACACAA TTTGACCTAT TAACCTATTC   781 AGGTTAGTAT AGGTTGGACG CTCAATATCG AGGTTTCTAC GACAGATGTC ATAGATGGCC   841 TCATTGTCTA CCATGAAGGC ACAATCAGAG TGCTCCAGGG TGGTGTGGGT GGGGTGGGGG 901 AGGTGTGGGA GTTG //
Primer: SV40pro_F_V2 Sequence file: RDB14140_A6Dpc.seq
>D03180A1_A6Dp_1_SV40pro_F_V2_H06_24.ab1     1 GGTCCCACTT CTTTTGGGAG GCCTTAGGCC TTTTGCAAAG ATCGATCAAA GAGACAGGAT    61 GAGGATCGTT TCGCATGATT GAACAAGATG GATTGCACGC AGGTTCTCCG GCCGCTTGGG   121 TGGAGAGGCT ATTCGGCTAT GACTGGGCAC AACAGACAAT CGGCTGCTCT GATGCCGCCG   181 TGTTCCGGCT GTCAGCGCAG GGGCGCCCGG TTCTTTTTGT CAAGACCGAC CTGTCCGGTG   241 CCCTGAATGA ACTGCAAGAC GAGGCAGCGC GGCTATCGTG GCTGGCCACG ACGGGCGTTC   301 CTTGCGCAGC TGTGCTCGAC GTTGTCACTG AAGCGGGAAG GGACTGGCTG CTATTGGGCG   361 AAGTGCCGGG GCAGGATCTC CTGTCATCTC ACCTTGCTCC TGCCGAGAAA GTATCCATCA   421 TGGCTGATGC AATGCGGCGG CTGCATACGC TTGATCCGGC TACCTGCCCA TTCGACCACC   481 AAGCGAAACA TCGCATCGAG CGAGCACGTA CTCGGATGGA AGCCGGTCTT GTCGATCAGG   541 ATGATCTGGA CGAAGAACAT CAGGGGCTCG CGCCAGCCGA ACTGTTCGCC AGGCTCAAGG   601 CGAGCATGCC CGACGGCGAG GATCTCGTCG TGACCCATGG CGATGCCTGC TTGCCGAATA   661 TCATGGTGGA AAATGGCCGC TTTTCTGGAT TCATCGACTG TGGCCGGCTG GGTGTGGCGG   721 ACCGCTATCA GGACATAGCG TTGGCTACCC GTGATATTGC TGAAGAACTT GGCGGGCGAA   781 TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCCGATTCGC AGCGCATCGC   841 CTTTCTATCG CCTTCTTGAC GAGGTTTCTT TCTGAGCGGG GACTCTGGGG GGTTCGAAAA   901 TGAACCGAAC CAAGCGAACG CCCCAACCCT GCCAATCACG AGATTTCGAT TTCCCACCGG   961 CCCGCCTTTC TAATGAAAGG GTTGGGGCTT TCGGAAATCG TTTTTTCCGG GACGTCCGGG  1021 CCTGGGATTG AATCCCTTCC CAGCGGCGGG GGAATCTCAT GGCTGGGGAG TTTCCTTCGC  1081 CCACCCCTAA GGGGGGAGGG CTTAAGCTTG CAAAACTACT CGGGAATGGG AGAGATACCA 1141 ATGTAAACGA CG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl