A plasmid for construction of C-terminal mAID tagging donors.
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External Database Arabidopsis thaliana AtIAA17 Reference sequence |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Natsume, T., Cell Reports, 15: 210-218, 2016). This DNA clone is used for academic research purpose only. This is not to be used on a commercial basis. For use of this DNA clone except for internal academic research, the RECIPIENT must contact a NIG license representative at chizai@nig.ac.jp. |
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Ordering | Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link] |
提供条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Natsume, T., Cell Reports, 15: 210-218, 2016)。学術研究にのみ限定、商業営利目的利用を禁ずる。学術内部利用以外の利用の際は、国立遺伝学研究所 (chizai@nig.ac.jp) に相談すること。 |
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提供依頼 | 提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB13929 | pMK286 (mAID-NeoR) | DNA solution |
Materials & Methods section:
The pMK286 (mAID-NeoR) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB13929). |
Reference section:
Natsume, T., Kiyomitsu, T., Saga, Y., Kanemaki, M.T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Auxin Inducible Degron (AID) System (English text) |
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Featured content | Auxin Inducible Degron (AID) System (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13929_A6B8p1-4.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: pUC-M13_-100 (Pr0310) Region: insert5'(T7 pro,Linker,mAID,SV40 pA,f1 ori) Sequence file: RDB13929_A6B8g.seq |
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>13929_13929_B3BoF_pUC-M13_-100_F01_06_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNGCNNGNNN TTTCCNNGTC NCGNCGTTGT AAAACGACGG 61 CCAGTGAGCG CGCGTAATAC GACTCACTAT AGGGCGAATT GGAGCTCCCC GGGATCCGGT 121 GCAGGCGCCA AGGAGAAGAG TGCTTGTCCT AAAGATCCAG CCAAACCTCC GGCCAAGGCA 181 CAAGTTGTGG GATGGCCACC GGTGAGATCA TACCGGAAGA ACGTGATGGT TTCCTGCCAA 241 AAATCAAGCG GTGGCCCGGA GGCGGCGGCG TTCGTGAAGG TATCAATGGA CGGAGCACCG 301 TACTTGAGGA AAATCGATTT GAGGATGTAT AAATAATCTA GATAACTGAT CATAATCAGC 361 CATACCACAT TTGTAGAGGT TTTACTTGCT TTAAAAAACC TCCCACACCT CCCCCTGAAC 421 CTGAAACATA AAATGAATGC AATTGTTGTT GTTAACTTGT TTATTGCAGC TTATAATGGT 481 TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG CATTTTTTTC ACTGCATTCT 541 AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTAACGCG TAAATTGTAA GCGTTAATAT 601 TTTGTNNNNN NTCGCGTTAA ATTTTTGTTA AATCANCTCA TTTTTTAACC AATAGGCCGA 661 AAATCGGCAA AATCCCTTAT AAATCAAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT 721 CCNGTTTGGA ACAAGAGTCC ACTATTANNN NNNCGTGGAC TCNNACGTNN AAGGGCGAAA 781 AAANCNTCTA TCAGGNNGAT GGCCCNNTAC GTGNANCCAT CNCCCNTANT CAAGTTTTTT 841 NGGGGGNNNA NGTGNCNTNN NNNNNNNAAA TNCGGAACCC NNAAAGGGNN NNCCCCNNNA 901 NTTTNNAGCT TGNNNNGGNN AANNCNNCNN ANCNTGGNNN ANNAAAGNNA NNGNNNNNNN 961 NNN // |
Primer: AFR (Pr0324) Region: insert3'(T3 pro,TK pA,NeoR_KanR) Sequence file: RDB13929_A6B8h.seq |
>13929_13929_B3BoF_AFR_G01_07_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NGCNNNGCGC GCANTTAACC CTCACTAAAG GGAACAAAAG 61 CTGGGTACCG GATCCAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC 121 ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG 181 TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG 241 AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT 301 GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT 361 CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA 421 CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG 481 CTATGTCCTG ATAGCGGTCC GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC 541 GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT 601 CGCCGTCGGG CATGCTCGCN NNNNNCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT 661 GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT 721 CGATGCGATG TTTCGCTNGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC 781 GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG ANCANNGTGA GATGACAGGA 841 GATCCTGCCC CGGNACTTCG CCNNATAGCA GCCAGTCCCT TCCCGCTTCA NNGNCNNCGT 901 CNNNCACANC TGCNCAAGGA ACGCCNGTCG NNNNNNNGCC NCGATANNCN NCGCNNCNNN 961 NNCNTNNNNN NTNNNNTNNN GNNNNNNNNN N // |
Primer: SV40pro_F_V2 (Pr0154) Region: insert mid(Neo_KanR) Sequence file: RDB13929_A6B8i.seq |
>D03070A13_A6B8_1_SV40pro_F_V2_D10_10.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNGCTTTTG CAAGNNCGAT CAAGANNNAG GNTGAGGNTC 61 GTTTCGCATG ATTGAACNNN ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG 121 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG 181 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA 241 TGAACTGCAA GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC 301 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC 361 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA 421 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA 481 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCGATC AGGATGATCT 541 GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC GCCAGGCTCA AGGCGAGCAT 601 GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC TGCTTGCCGA ATATCATGGT 661 GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG CTGGGTGTGG CGGACCGCTA 721 TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA 781 CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCN 841 NCTTCNNGAC GAGTTCTTCT GANCGGGANT CTGGGGGTTC NAAATGNNCG ACCNNGCGNC 901 GCCCNANCCT GCCNNNNCNN NATTTNNANN NCNNCGCCGC CNNCTATGAA ANNNTNNGGN 961 CTTCGGANNN NTTTTNNNGG ANNNNNGNCN GGNTGATCCN NCNNNCGNNG GGNNNNNNNN 1021 NN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Natsume, T., Rapid protein depletion in human cells by auxin-inducible degron tagging with short homology donors. Cell Rep. 15 (1): 210-218 (2016). PMID 27052166. [link to RRC of NBRP] |
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reference | Chen, F.X., PAF1 regulation of promoter-proximal pause release via enhancer activation. Science 357 (6357): 1294-1298 (2017). PMID 28860207. |
reference | Natsume, T., Acute inactivation of the replicative helicase in human cells triggers MCM8-9-dependent DNA synthesis. Genes Dev. (2017). PMID 28487407. |
reference | Devrekanli, A., Conditional Budding Yeast Mutants with Temperature-Sensitive and Auxin-Inducible Degrons for Screening of Suppressor Genes. Methods in Molecular Biology 1369: 257-278 (2016). PMID 26519318. |
reference | Nishimura, K., Rapid Depletion of Budding Yeast Proteins via the Fusion of an Auxin-Inducible Degron (AID). Curr. Protoc. Cell Biol. \64: 20.9.1-16 (2014). PMID 25181302. |
2024.03.26
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