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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

FLAG-His-eIF5A(K50A) on pcDNA3.1 (unhypusinated form)

Expression vector of human eIF5A, unhypusinated form.

Catalog number RDB13752
Resource name FLAG-His-eIF5A(K50A) on pcDNA3.1 (unhypusinated form)
Clone info. Expression vector of FLAG- and His-tagged mutant eIF5A (K50A), which cannot be hypusinated.
Comment Expression was confirmed by the depositor with Western blot.
Vector backbone pcDNA3.1 Directional TOPO (plasmid)
Size of vector backbone 5.5 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cells)
Gene/insert name human eIF5A cDNA
Depositor|Developer Seko, Yoshinori |
Other clones in our bank
External Database
human eIF5A
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Seko, Y., Sci. Rep. 5: 13737 2015.)
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Seko, Y., Sci. Rep. 5: 13737 2015.)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB13752 FLAG-His-eIF5A(K50A) on pcDNA3.1 (unhypusinated form) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Seko, Y., Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis. Sci. Rep. 5: 13737 (2015). PMID 26348594.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13752_A6A5p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB13752_A6A5a.seq check
          >D02955A3_A6A5_7_CMV_Forward_D09_12.ab1
              1 CGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
            121 TCACTATAGG GAGACCCAAG CTGGCTAGTT AAGCTTGGTA CCGAGCTCGG ATCCAGTACC
            181 CTTCACCGAA TTCAAAATGG CAGATGACTT GGACTTCGAG ACAGGAGATG CAGGGGCCTC
            241 AGCCACCTTC CCAATGCAGT GCTCAGCATT ACGTAAGAAT GGCTTTGTGG TGCTCAAAGG
            301 CCGGCCATGT AAGATCGTCG AGATGTCTAC TTCGAAGACT GGCGCGCACG GCCACGCCAA
            361 GGTCCATCTG GTTGGTATTG ACATCTTTAC TGGGAAGAAA TATGAAGATA TCTGCCCGTC
            421 AACTCATAAT ATGGATGTCC CCAACATCAA AAGGAATGAC TTCCAGCTGA TTGGCATCCA
            481 GGATGGGTAC CTATCACTGC TCCAGGACAG CGGGGAGGTA CGAGAGGACC TTCGTCTCCC
            541 TGAGGGAGAC CTTGGCAAGG AGATTGAGCA GAAGTACGAC TGTGGAGAAG AGATCCTGAT
            601 CACGGTGCTG TCTGCCATGA CAGAGGAGGC AGCTGTTGCA ATCAAGGCCA TGGCAAAAGA
            661 TTACAAGGAC GACGATGACA AGCACCACCA TCACCATCAC TGACTCGAGT ATATAAGGGT
            721 CAAGACAATT CTGCAGATAT CCAGCACAGT GGCGGCCGCT CGAGTCTAGA GGGCCCGCGG
            781 TTCGAAGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGCG TACCGGTCAT
            841 CATCACCATC ACCATTGAGT TTAAACCCGC TGATCAGCCT CGACTGTGCC TTCTAGTTGC
            901 CAGCCATCTG TTGTTTGCCC CTCCCCCGTG CCCTTCCTTG ACCCTGGAAA GTGCCACTCC
            961 CACTGTCCCT TTCCTAATAA AATGAGGAAA TTTGCATCGC ATTGTCTGAG TAGTGTCATT
           1021 CTATTCCTGG GGGGTTGGGG TGGGGGCAGA CAGCCAGGGG GAAGAATGGT AGACCATAGC
          1081 AGCATGCTAC GATGCGTGGC TCTATGGCCT TCTGAGC
          //
          Primer: SV40pro_F
          Sequence file: RDB13752_A6A5b.seq check
          >D02955A3_A6A5_7_SV40pro_F_F09_18.ab1
              1 CCCTGTCTGG GACATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA
             61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG
            121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG
            181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG
            241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT
            301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA
            361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG
            421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT
            481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA
            541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG
            601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG
            661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG
            721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT
            781 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC
            841 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC
            901 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGC GAAATGACCG ACCAAGCGAC
            961 GCCCAAACCT GCCCATCACG AGATTTCGAT TTCCACCGCC CGCTTTCTAT GAAACGTTGG
           1021 CTTTCGGAAT CGTTTTCTGG ACGGCGGCTG CATGGATCCT TCAGCGCGGG GATCTCATGC
          1081 TGAGTCATGC TCACCCGAAC TGGTTTAATT GCAGGTCTTT ATTAATG
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Seko, Y., Secreted tyrosine sulfated-eIF5A mediates oxidative stress-induced apoptosis. Sci. Rep. 5: 13737 (2015). PMID 26348594.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl