Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII-EF-GFP-IRES-Puro

Lentivirus vector expressing GFP driven by EF-1 alpha promoter with puromycin resistance marker.

Sequence (full)	RDB12868zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone.

提供案内 (日本国内) [open/close]

提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12868_A9Gtp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168) Region: EF1a pro, attB1, EGFP 5' Sequence file: RDB12868_A9Gta.seq
>12868_12868_A9Gt_3_EF1a-C_F_C11_08_ABI24.ab1     1 NNNNNNNNNN NNTTTTGANT TNGGNNCTTG GTTCNTTCTC AAGCCTCAGA CAGTGGTTCA    61 AAGTTTTTTT CTTCCNTTTC AGGTGTCGTG AACACGCTAC CGGTCTCGAG AATTATCACA   121 AGTTTGTACA AAAAAGCAGG CTCCACCATG GTGAGCAAGG GCGAGGAGCT GTTCACCGGG   181 GTGGTGCCCA TCCTGGTCGA GCTGGACGGC GACGTAAACG GCCACAAGTT CAGCGTGTCC   241 GGCGAGGGCG AGGGCGATGC CACCTACGGC AAGCTGACCC TGAAGTTCAT CTGCACCACC   301 GGCAAGCTGC CCGTGCCCTG GCCCACCCTC GTGACCACCC TGACCTACGG CGTGCAGTGC   361 TTCAGCCGCT ACCCCGACCA CATGAAGCAG CACGACTTCT TCAAGTCCGC CATGCCCGAA   421 GGCTACGTCC AGGAGCGCAC CATCTTCTTC AAGGACGACG GCAACTACAA GACCCGCGCC   481 GAGGTGAAGT TCGAGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC   541 AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC   601 TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC   661 ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC   721 GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC 781 CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGANTTCG TGACCGCCGC CGG //
Primer: mVenus-M_F (Pr0574) Region: EGFP 3', attB2, chimeric intron, IRES Sequence file: RDB12868_A9Gtb.seq
>12868_12868_A9Gt_3_mVenus-M_F_A01_01_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGT GNACTTCNNG ATCCGCCACA ACATCGAGGA    61 CGGCAGCGTG CAGCTCGCCG ACNACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT   121 GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA   181 GAAGCGCGAT CACATGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGATCA CTCTCGGCAT   241 GGACGAGCTG TACAAGTCCG GACTCAGATC CTACTAGTAG GATCTCGAGA TATCTAGACC   301 CAGCTTTCTT GTACAAAGTG GTGATAATTC TGCAGCGGCC GCGGATCCTC TAGACTGGAA   361 TTAATTCGCT GTCTGCGAGG GCCAGCTGTT GGGGTGAGTA CTCCCTCTCA AAAGCGGGCA   421 TGACTTCTGC GCTAAGATTG TCAGTTTCCA AAAACGAGGA GGATTTGATA TTCACCTGGC   481 CCGCGGTGAT GCCTTTGAGG GTGGCCGCGT CCATCTGGTC AGAAAAGACA ATCTTTTTGT   541 TGTCAAGCTT GAGGTGTGGC AGGCTTGGGA TCTGGCCATA CACTTGAGTG ACAATGACAT   601 CCACTTTGCC TTTCTCTCCA CAGGTGTCCA CTCCCAGGTC CAACTGCAGG TCGAGCATGC 661 ATCTAGGGCG GCCAATTCCG CCCCTCTCCC CNNNNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro, 5'LTR, HIV-1 psi Sequence file: RDB12868_A9Gtc.seq
>12868_12868_A9Gt_3_CMV-Forward_C11_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNTG NNNCNNTGGG CGGTAGGCGT GTACGGTGGG AGGTCTATAT    61 AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC   121 TCTCTGGCTA ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC   181 AAGTAGTGTG TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT   241 AGTCAGTGTG GAAAATCTCT AGCAGTGGCG CCCGAACAGG GACTTGAAAG CGAAAGGGAA   301 ACCAGAGGAG CTCTCTCGAC GCAGGACTCG GCTTGCTGAA GCGCGCACGG CAAGAGGCGA   361 GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT TTTGACTAGC GGAGGCTAGA AGGAGAGAGA   421 TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG GAGAATTAGA TCGCGATGGG AAAAAATTCG   481 GTTAAGGCCA GGGGGAAAGA AAAAATATAA ATTAAAACAT ATAGTATGGG CAAGCAGGGA   541 GCTAGAACGA TTCGCAGTTA ATCCTGGCCT GTTAGAAACA TCAGAAGGCT GTAGACAAAT   601 ACTGGGACAG CTACAACCAT CCCTTCAGAC AGGATCAGAA GAACTTAGAT CATTATATAA   661 TACAGTAGCA ACCCTCTATT GTGTGCATCA AAGGATAGAG ATAAAAGACA CCAAGGAAGC   721 TTTAGACAAG ATAGAGGAAG AGCAAAACAA AAGTAAGACC ACCGCACAGC AAGGGCCGCT   781 NATCTTCAGA CCTGGAGGAN GAGATATGAG GGANNATTGG AGAAGTGAAT TATATAAATA 841 TNAAGTAGTA AAAAANTGAN NCNTTNNGAG TAGCACCCNC CNA //
Primer: PRE_rev (Pr0250) Region: PRE, PuroR, IRES Sequence file: RDB12868_A9Gtd.seq
>12868_12868_A9Gt_3_PRE_rev_B11_05_ABI24.ab1     1 NNNNNNNNNN NNGNNNNNGN NCNNNNTAGT TAAGAATACC NGTCAATCTT TCACAAATTT    61 TGTAATCCAG AGGTTGATTA TCGATAAGCT TGATCCCTCG ATGTTTCAGG CACCGGGCTT   121 GCGGGTCATG CACCAGGTGC GCGGTCCTTC GGGCACCTCG ACGTCGGCGG TGACGGTGAA   181 GCCGAGCCGC TCGTAGAAGG GGAGGTTGCG GGGCGCGGAG GTCTCCAGGA AGGCGGGCAC   241 CCCGGCGCGC TCGGCCGCCT CCACTCCGGG GAGCACGACG GCGCTGCCCA GACCCTTGCC   301 CTGGTGGTCG GGCGAGACGC CGACGGTGGC CAGGAACCAC GCGGGCTCCT TGGGCCGGTG   361 CGGCGCCAGG AGGCCTTCCA TCTGTTGCTG CGCGGCCAGC CGGGAACCGC TCAACTCGGC   421 CATGCGCGGG CCGATCTCGG CGAACACCGC CCCCGCTTCG ACGCTCTCCG GCGTGGTCCA   481 GACCGCCACC GCGGCGCCGT CGTCCGCGAC CCACACCTTG CCGATGTCGA GCCCGACGCG   541 CGTGAGGAAG AGTTCTTGCA GCTCGGTGAC CCGCTCGATG TGGCGGTCCG GGTCGACGGT   601 GTGGCGCGTG GCGGGGTAGT CGGCGAACGC GGCGGCGAGG GTGCGTACGG CCCGGGGGAC   661 GTCGTCGCGG GTGGCGAGGC GCACCGTGGG CTTGTACTCG GTCATGGAAG GTCGTCTCCT   721 TGTGGGTTGT GGCAAGCTTA TCATCGTGTT TTTCAAAGGA AAACCACGTC CCCGNGGTTC   781 GGGNGGCCTA GACGTTTTTT TAACCTCGAC TAAACACATG TAAAGCATGT GCACCGAGGC 841 CCCAGNTCAG ATCCNATACA ANGGGGNTNN CTTCNGGGCN TCCNNCAGCC CC //
Primer: f1ori_R3 (Pr0142) Region: bGH p(A), 3'LTR Sequence file: RDB12868_A9Gte.seq
>12868_12868_A9Gt_3_f1ori_R3_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNT TNNNCCTCAN AAGCCNTAGA GCCCNNCCGC    61 ATCCCCAGCA TGCCTGCTAT TGTCTTCCCA ATCCTCCCCC TTGCTGTCCT GCCCCACCCC   121 ACCCCCCAGA ATAGAATGAC ACCTACTCAG ACAATGCGAT GCAATTTCCT CATTTTATTA   181 GGAAAGGACA GTGGGAGTGG CACCTTCCAG GGTCAAGGAA GGCACGGGGG AGGGGCAAAC   241 AACAGATGGC TGGCAACTAG AAGGCACAGT CGAGGCTGAT CAGCGGGTTT AAACGGGCCC   301 TGCTAGAGAT TTTCCACACT GACTAAAAGG GTCTGAGGGA TCTCTAGTTA CCAGAGTCAC   361 ACAACAGACG GGCACACACT ACTTGAAGCA CTCAAGGCAA GCTTTATTGA GGCTTAAGCA   421 GTGGGTTCCC TAGTTAGCCA GAGAGCTCCC AGGCTCAGAT CTGGTCTAAC CAGAGAGACC   481 CAGTACAGTC CGGATGCAGC TCTCGGGCCA TGTGATGAAA TGCTAGGCGG CTGTCAAACC   541 TCCACTCTAA TACTTCTCTC TCCGGGTCAT CCATCCCATG CAGGCTCACA GGGTGTAACA   601 AGCGGGTGTT CTCTCCTTCA TTGGCTTCTT CTACCTTCTC TTGCTCAACT GGTACTAGCT   661 TGTAGCACCA TCCAAAGGTC AGTGGATATC TGATCCCTGG CCCTGGTGTG TAGTTCTGCC   721 AATCAGGGAA GTAGCCTTGT GTGTGGTAGA TCCACAGATC AAGGATATCT TGTCTTCGTT   781 GGGAGTGAAT TAGCCCTTCC AGTCCCCCCT TTTCTTTTNA AAAAGTGGCT AAGATCTACA   841 GCTGCCTTGT AAGTCATTGG NCTTAAAGGT ACCTGANGTG TGACTGGAAA ANCCNCCTCC   901 TCCTCCTCTT GTGCTTCTAG CCAGGCACNA TCAGCATTGG NAGCTGCTGT ATTGCTACTT   961 GNGANTGCTC CNTGTTTTTC TAGGTCTCGA CGGNNNCNAT GNGGGGANGC NGNCNNAANG 1021 GANNNNCNNN NNNNTCNNNN GGNNNNANNN NNNNNNN //
Primer: EF1a-N_R (Pr0024) Region: EF1a pro, cPPT_CTS, RRE Sequence file: RDB12868_A9Gtf.seq
>12868_12868_A9Gt_3_EF1a-N_R_D11_11_ABI24.ab1     1 NNNNNNNNNN NNNNNNGGCG NNGTGCGCTC TGCCNNCTGN CGGGCACCGG ANCCTCACGA    61 AGCTTGAATT AATTCAAAGT GAATTTCTGC TGTCCCTGTA ATAAACCCGA AAATTTTGAA   121 TTTTTGTAAT TTGTTTTTGT AATTCTTTAG TTTGTATGTC TGTTGCTATT ATGTCTACTA   181 TTCTTTCCCC TGCACTGTAC CCCCCAATCC CCCCTTTTCT TTTAAAATTG TGGATGAATA   241 CTGCCATTTG TAGATCCGTT CACTAATCGA ATGGATCTGT CTCTGTCTCT CTCTCCACCT   301 TCTTCTTCTA TTCCTTCGGG CCTGTCGGGT CCCCTCGGGG TTGGGAGGTG GGTCTGAAAC   361 GATAATGGTG AATATCCCTG CCTAACTCTA TTCACTATAG AAAGTACAGC AAAAACTATT   421 CTTAAACCTA CCAAGCCTCC TACTATCATT ATGAATAATT TTATATACCA CAGCCAATTT   481 GTTATGTTAA ACCAATTCCA CAAACTTGCC CATTTATCTA ATTCCAATAA TTCTTGTTCA   541 TTCTTTTCTT GCTGGTTTTG CGATTCTTCA ATTAAGGAGT GTATTAAGCT TGTGTAATTG   601 TTAATTTCTC TGTCCCACTC CATCCAGGTC GTGTGATTCC AAATCTGTTC CAGAGATTTA   661 TTACTCCAAC TAGCATTCCA AGGCACAGCA GTGGTGCAAA TGAGTTTTCC AGAGCAACCC   721 CAAATCCCCA GGAGCTGTTG ATCCTTTANG TATCTTTCCA CAGCCAGGAT TCTTGCCTGG   781 ANCTGCTTGA TGCCCCAGAC TGTGAGTTGC AACAGATGCT GTTGCGCCTC AATAGCCCTC 841 ANCNNATTGT TCTGCTGCTG CAC //
Primer: SV40pro_F (Pr0110) Region: SV40pro_ori, Zeo, SV40 p(A) Sequence file: RDB12868_A9Gtg.seq
>12868_12868_A9Gt_3_SV40pro_F_F11_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNTGNNG NNGNTTTTTT GGANGCCTAG GCTTTTGCAA    61 AAAGCTCCCG GGNNCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA   121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC   181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC   241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC   301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC   361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG   421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG   481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG   541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC   601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG   661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT   721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC   781 AAACTCATCA ATGTATCTTA TCATGTCTGT ATANCGTCNA CCTCTAGCTA GAGCTTGGCG   841 TAATCATGGT CATAGCTGTT TCCTGTGTGA ANTTGTTATC CGCTCACAAT TCCNCACAAC 901 ATACGANCCN GAAGCATAAA GNGTAAAG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl