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pcDNA3Flag-hp38delta

Expression vector of human p38delta MAPK cDNA, FLAG-tagged

Catalog number RDB12363
Resource name pcDNA3Flag-hp38delta
Clone info. Expression vector of human p38delta MAPK cDNA, FLAG-tagged.
Comment This clone contains a cDNA fragment corresponding to the 71nt to 1168nt of NM_002754.5. Known difference (at least) : A to C substitution at 121nt (17a.a. Thr, synonym).
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5.4 kb
Gene/insert name Human p38delta cDNA
Depositor|Developer Sudo, Tatsuhiko |
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External Database
Human p38delta

          Reference sequence
            
           

          Distribution information

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          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. Research purpose only.
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          RDB12363 pcDNA3Flag-hp38delta DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pcDNA3Flag-hp38delta was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12363).

          Reference section:

          Kondoh, Y., Honda, K., Hiranuma, S., Hayashi, T., Shimizu, T., Watanabe, N., Osada, H., Comparative chemical array screening for p38gamma/delta MAPK inhibitors using a single gatekeeper residue difference between p38alpha/beta and p38gamma/delta. Sci. Rep. 6: 29881 (2016). PMID 27431267.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content MAPK p38 inhibitor screening (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12363_A4B5p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,insert 5'(FLAG,p38 delta 5')
          Sequence file: RDB12363_A4B5a.seq check
          >12363_12363_B2A7F_1_CMV-Forward_A03_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNANNNGTG GGNGGTCTAT ATAAGCAGAG CTCTCTGGCT
             61 AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT ACGACTCACT ATAGGGAGAC
            121 CCAAGCTTGC CACCATGGAC TACAAGGACG ACGACGATAA ATCGATGAGC CTCATCCGGA
            181 AAAAGGGCTT CTACAAGCAG GACGTCAACA AGACCGCCTG GGAGCTGCCC AAGACCTACG
            241 TGTCCCCGAC GCACGTCGGC AGCGGGGCCT ATGGCTCCGT GTGCTCGGCC ATCGACAAGC
            301 GGTCAGGGGA GAAGGTGGCC ATCAAGAAGC TGAGCCGACC CTTTCAGTCC GAGATCTTCG
            361 CCAAGCGCGC CTACCGGGAG CTGCTGCTGC TGAAGCACAT GCAGCATGAG AACGTCATTG
            421 GGCTCCTGGA TGTCTTCACC CCAGCCTCCT CCCTGCGCAA CTTCTATGAC TTCTACCTGG
            481 TGATGCCCTT CATGCAGACG GATCTGCAGA AGATCATGGG GATGGAGTTC AGTGAGGAGA
            541 AGATCCAGTA CCTGGTGTAN CAGATGCTNN NNNGCCTTAA GTACATCCAC TCTGCTGNNG
            601 TCGTGCACAN GGNNCTGANN NCAGGNNNNN NGGCTGTGAA TGANGNNTGT GANCTGNNNA
          661 NTCTGGNNNN TTTGGGNNNN NNNNNACNNG NNNNNNNNCN NNNNNNNNN
          //
          Primer: bGH_rev3 (Pr0885)
          Region: insert 3'(p38 delta 3')
          Sequence file: RDB12363_A4B5b.seq check
          >12363_12363_B2A7F_1_bGH_rev3_H01_08_ABI08.ab1
              1 NNNNNNNNNN NNNNGGNNNN NNNNNNNNNA NNAGNTGGCT GGCAACTAGN NAGGCACAGT
             61 CGAGGCTGAT CAGCGAGCTC TAGCATTTAG GTGACACTAT AGAATAGGGC CCTCTANATG
            121 AGTCCCTACA GCTTCATGCC ACTCCGGCGC CGTGAGTCCT TCCGGGCAAT GGGGCTGAAG
            181 TTCACAATCT CCTTGTAGAT GTGCTGCTTC CATTCATCCA CTGTGAGTTT CTCGTGTTCT
            241 AAGGAATCAT CAAACGGCTG CTGGGCCTCC GTCTCTTCCT CAGGGTCCCG GAAGGGTTCA
            301 AAGAAGGGAT GGGTGAGGGC CTGCGCGGCC GTCAGGCGCT TGTCCACGTC TAGCTCCAGC
            361 ATCTTCTCCA GCAGGTCCGC AGCCTGGGGG CTGGCCCGTG GGAACAGCTG AGTGAAATCC
            421 TTCCTGGGGG TCTGTGGCAG GGACTGGATG TAGGATTTGG CCGCTTTGTC GTTCAGCTTC
            481 TGCACAAACT CCGTGCCAGG CACCCCGGTC ACTTTCAGGA TCTGGGTCAG CTGGTCCAGG
            541 TAATCTTTCC CCTTGAACAG AGTTTTCCCT GTCNNCATCT CTGCCATGNN ACAGCCCANA
            601 GACCANANGT CNNCTGTCTG GNTGTAGTGC ATNCNNCTGA GGATCACNTN NNGGGGCTCG
          661 GTACNNNNNG GTCACCNNNN NNNCNNNNNN NNNN
          //
          Primer: p38delta-M_R (Pr1103)
          Region: insert mid(p38 delta mid)
          Sequence file: RDB12363_A4B5c.seq check
          >12363_12363_B2A7F_1_p38delta-M_R_H01_08_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGGCCGCTTT GTCGTTNNGC TTCTGCACAA ACTCCGTGCC
             61 AGGCACCCCG GTCACTTTCA GGATCTGGGT CAGCTGGTCC AGGTAATCTT TCCCCTTGAA
            121 CAGAGTTTTC CCTGTCAGCA TCTCTGCCAT GATACAGCCC ACAGACCAGA TGTCCACTGT
            181 CTGGTTGTAG TGCATCCAGC TGAGGATCAC CTCGGGGGCT CGGTACCAGC GGGTCACCAC
            241 GTAGCCAGTC ATCTCGGCGT CTGCATGTCG CGCCAGCCCA AAATCCAGAA TCTTCAGTTC
            301 ACAGTCCTCA TTCACAGCCA GGTTGCCTGG CTTCAGGTCC CTGTGCACGA CCCCAGCAGA
            361 GTGGATGTAC TTAAGGCCTT TGAGCATCTG ATACACCAGG TACTGGATCT TCTCCTNNNN
            421 NNACTCCATC CCCATGATCT TCNGNNNATC CGTCTGCATG AAGGGCATCA CCANNTANAN
          481 NNNATAGAAG TTGCGCNNGG AGGANNNNGG NNNGAANANN NNNANNANNN NNNNNNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: Sv40 pro_ori,NeoR
          Sequence file: RDB12363_A4B5d.seq check
          >12363_12363_B2A7F_1_SV40pro_ori_F_C03_09_ABI24.ab1
              1 NNNNNCNGNN CTATTCNNGA AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
             61 AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT CAAGAGACAG GATGAGGATC
            121 GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG
            181 GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC TCTGATGCCG CCGTGTTCCG
            241 GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC GACCTGTCCG GTGCCCTGAA
            301 TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC ACGACGGGCG TTCCTTGCGC
            361 AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG CTGCTATTGG GCGAAGTGCC
            421 GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG AAAGTATCCA TCATGGCTGA
            481 TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC CCATTCGACC ACCAAGCGAA
            541 ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT CTTGTCNATC ANGATGATCT
            601 GGACGAAGAG CATCANGGGC TCGCGCCNGC CNAACTGNTC GCCNNGCTNN ANGCGCNNAT
            661 GNCCGANNGC GANNNNCTCN TCNNGACCNN NGGNNNNGNC NGNNNNNNNA NNNNNNNNNG
          721 NNNGNNAANN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Kondoh, Y., Comparative chemical array screening for p38gamma/delta MAPK inhibitors using a single gatekeeper residue difference between p38alpha/beta and p38gamma/delta. Sci. Rep. 6: 29881 (2016). PMID 27431267.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl