Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA3Flag-hp38gamma

Expression vector of human p38gamma MAPK cDNA, FLAG-tagged

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12361_A4B5p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,insert 5'(FLAG,p38 gamma 5') Sequence file: RDB12361_A4B5a.seq
>12361_12361_B2A7F_2_CMV-Forward_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNCNNNTGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA    61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA   121 CGACTCACTA TAGGGAGACC CAAGCTTGCC ACCATGGACT ACAAGGACGA CGACGATAAA   181 TCGATGAGCT CTCCGCCGCC CGCCCGCAGT GGCTTTTACC GCCAGGAGGT GACCAAGACG   241 GCCTGGGAGG TGCGCGCCGT GTACCGGGAC CTGCAGCCCG TGGGCTCGGG CGCCTACGGC   301 GCGGTGTGCT CGGCCGTGGA CGGCCGCACC GGCGCTAAGG TGGCCATCAA GAAGCTGTAT   361 CGGCCCTTCC AGTCCGAGCT GTTCGCCAAG CGCGCCTACC GCGAGCTGCG CCTGCTCAAG   421 CACATGCGCC ACGAGAACGT GATCGGGCTG CTGGACGTAT TCACTCCTGA TGAGACCCTG   481 GATGACTTCA CGGACTTTTA CCTGGTGATG CCGTTCATGG GCACCGACCT GGGCAAGCTC   541 ATGAAACATG AGAAGCTAGG CGAGGACCGG ATCCNGTTCC TCGTGTACCA GATGCTGAAG   601 GGGCTGANGT ATATCNNCGC TGCCNGCATC ATCCNCAGAG ACCTGAAGCC CGGCAACNTG 661 GNTGNGAACN AANACTGNGA NCTGNNNNNC CNNNNNTTNN NCNNNNNNNN NN //
Primer: bGH_rev3 (Pr0885) Region: insert 3'(p38 gamma 3') Sequence file: RDB12361_A4B5b.seq
>12361_12361_B2A7F_2_bGH_rev3_B02_05_ABI24.ab1     1 NNNNNNNNNN GNNNNNNNNN NNNCTNNAAN GNGNNNNNNC GAGGNCTGAT CNGCGAGCTC    61 TAGCATTTAG GTGACACTAT AGAATAGGGC CCTCTAGATC ACAGAGGCGT CTCCTTGGAG   121 ACCCTGGCCC CCAGCTGCCG GGGAGGCTTG AAGCTGAGCA CCTCTTTGTA AGTAACACGC   181 TTCCATTCAT CCAGTGTGCG GTCAACGTCG TCAAAGGAGT CATCATACTT CTGGACCTGG   241 GGCTCATCTT CCGTGTCGTG CAGGGACTCG AAGTAGGGAT GGGCCAGCGC CTCGCCTGCC   301 GTCACCCGCT GCTCCGCGTC CAGCACCAGC ATCTTCTCCA GGAGGTTCAC AGCCAGAGGG   361 CTTGCATTGG TCAGGATAGA GGCAAAATCC TTCTTCTCCA ATTCGGGGAG GCCCTTCATG   421 TAGTTCTTGG CCTCATCGCT CTGCAGCCGC TGCACAAACT CAGCCGGAGG CGTCCCCNNN   481 NNNTTCATGA TNNNNNNCAG CTGGNCNNNN NGGTCGCTGC CCCTTGANNN NCGTCTTGNN   541 NGNGATNNNN NCCNCCCNTG ATGCAGCCCA CGGANCNANA NNGNCCNNCG NCTGCNNNGN   601 ANNNNNNTCC NATTNNNNAT GANNNTNNNG NNCNCGGTAN NNNNNNNNTN NNNCNCNNNN 661 NNAGTCNNNN NNNNNGNNNN GNCNGNNNGG NNNNNNNNNN //
Primer: p38gamma-M_R (Pr1102) Region: insert mid(p38 gamma mid) Sequence file: RDB12361_A4B5c.seq
>12361_12361_B2A7F_2_p38gamma-M_R_F03_18_ABI24.ab1     1 NNNNNNNNNN NNNNNCNNNT CTGNNGCCGC TGCACNAACT CNGCCNNGNN NNNNCCCCGT    61 CACCTTCATG ATCTCCTTCA GCTGGTCCAG GTGGTCGCTG CCCTTGAACA GCGTCTTGCC   121 TGTGATCATC TCCGCCATGA TGCAGCCCAC GGACCAGATG TCCACCGTCT GCGTGTAGCG   181 CATCCAATTC AAGATGACCT CGGGAGCCCG GTACCACCGG GTCACCACGT ACCCAGTCAT   241 CTCACTGTCT GCCTGCCTGG CCAGGCCGAA GTCCAGGATC TTCAGCTCAC AGTCTTCGTT   301 CACAGCCAGG TTGCCGGGCT TCAGGTCTCT GTGGATGATG CCGGCAGCGT GGATATACCT   361 CAGCCCCTTC AGCATCTGGT ACACGAGGAA CTGGATCCGG TCCTCGCCTA GCTTCTCATG   421 TTTCATGAGC TTGCCCAGGT CGGTGCCCAT GAACGGCATC ACCAGGTAAA AGTCCGTGAA   481 GTCATCCNGG GTCTCATCAG GAGTGAATAC GTCCAGCAGC CCGATCACGT TCTCGTGGCG   541 CATGTGCTTG AGCAGGCGCA GCTCGCGGTA NGCGCGCTTG GCGAACAGCT CGNACTGGAA   601 NGGCCGATNC NGCTTCTTGA TGGNCNCCTT ANCNNCNGNG CGGCCNTCCN CNGNCNNANC 661 NCNCCGCNNN NNNNNNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori,NeoR Sequence file: RDB12361_A4B5d.seq
>12361_12361_B2A7F_2_SV40pro_ori_F_C02_08_ABI24.ab1     1 NNNNNNNNNN NNTCTGNNNN NNNCNNGAAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC    61 TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA   121 TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG   181 GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC   241 GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT   301 GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT   361 CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC   421 GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC   481 ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC   541 CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCNGATGG AAGCCGGTCN TGNCGATCAN   601 GATGNNCTGG ACGANNAGCA TCNNGGGCTN NCGCCNGCCG AANTGNTCGC CNNGCTCNAN   661 GGNGCGNANG CCCNANNNCN ANNNNCTCNT CNNGACCNNN NGNNNANNNC NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl