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HA-Amot130-m5/pcDNA3.1-pA83

Expression clone of mouse Amot (p130), mPY123 mutation

Catalog number RDB12206
Resource name HA-Amot130-m5/pcDNA3.1-pA83
Alternative name HA-Amot-mPY123
Clone info. Expression clone of mouse Amot (p130), three mutations in PY motifs (mPY123). HA-tag.
Comment See Fig. 4A of Hirate et al., Curr. Biol. 23: 1181-1194 (2013). The expression vector has a stretch of poly(A).
Vector backbone pcDNA3.1-pA83 (Plasmid)
Size of vector backbone 5.9 kb
Selectable markers Amp^r
Gene/insert name Mouse Amot cDNA
Depositor|Developer Sasaki, Hiroshi |
Other clones in our bank

External Database
Mouse Amot

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
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          RDB12206 HA-Amot130-m5/pcDNA3.1-pA83 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12206_A5Hpp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB12206_A5Hpa.seq check
          >12206_12206_A5Hp_CMV-Forward_G11_20.ab1
              1 GCTATCCACT TCCGCCCCCC ATTGAAGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
             61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
            121 AATACGACTC ACTATAGGGA GACCCAAGCT GGCTAGTTAA GCTAGCTTGC CGCCACCATG
            181 TACCCATACG ACGTTCCAGA TTACGCTGGA TCCAGAAGTT CTGACGATCA GCCAAGTGGA
            241 GGAACGACGG TACTGCAACG GCTGCTTCAA GAGCAGCTTC GCTATGGCAA TCCCAGTGAG
            301 AATCGCAGTC TGCTTGCCAT ACACCAGCAA GCCACAGGGA ATAGCTCTCC ATTTTCCACG
            361 GGCAGTGGGA ACCAAGGCCC TCAGAATGAT GTATTGAGTT CCCAGGATCA CCACCAGCAG
            421 CAGCTTGTGG CACACCCTGC TCGCCAAGAG CCCCAGGGTC AGGAAATCCA GTCAGAAAAC
            481 GGCGTCATGG AGAAGCAGCT GTCCCCTCGA ATGCAGAATA ACGAAGAATT CGCAACCCAT
            541 GAAGAGGCCA AGGTCCAGTC CCAGTACTTC AGGGGCCAGC AGCATGCTAG TGTTGGGGCT
            601 GCTTTCTATG TCACAGGAGT CACCAACCAG AAGATGAGAA CCGAGGGACG CCCGTCAGTC
            661 CAGCGCCTCA CGCCCGGGAA GATGCACCAA GATGAAGGCC TTCGAGATCT TAAGCAAGGG
            721 CATGTCCGAT CCTTGAGTGA AAGACTGATG CAGATGTCAT TGGCTACTAG TGGAGTTAAA
            781 GCCCACCCAC CTGTTACCAG TGCTCCTCTC TCCCCACCCA CAACCCAATG ATCTCTACAA
            841 GAATGCCACC AGTTCCAGTG AATTCTACAA GGCCCAAAGG GCCACCTCCA AGCCAACATA
            901 GTCTGAAGGG CATGGAAACA CCGAGGTCCC GGATCCGAAC ACCCTTTCAA GGGCGTGCCA
            961 TCCCAGTCTG TAGTGTGTAA GTCCAAAGAG CCAGGGCACT TCTATAGTGA GCATCGCCTG
           1021 AACCCAGCTA GGAGAACAGA GGGGGCAACT GATGAGTATC AGCCACGGGT ACCGAGCATG
           1081 GGGCAGCCAA GGCAACACAG GGACATTCCT CCTTCGCTTG TCTTTTGGTC AGTCAGGAAA
          1141 CTTCTTCAAC CCTTCCATAT GAGACCTTAA CCATTCCTTT CCCCTTGACA
          //
          Primer: mAmot-M#2
          Sequence file: RDB12206_A5Hpb.seq check
          >12206_12206_A5Hp_mAmot-M#2_C09_09.ab1
              1 TGGGTTATTG CAGACTGCTG AGCCGAGGCA TAGCTGAGTA GGCTTCTCCT GGCTGCTGCT
             61 GCGCTGAAGT CAGGCTGGCC TGGGCGTGAC GGTAGTGGTC CCCCTGATGA CTCTGACTCG
            121 AAAGAAGATG CTGCTGATGC CTTGACAGGT GAGCAGAGTG GTCACCTTGG TTCGGGACCA
            181 GGTGTTGACC AGGAGGCAAT CTAGTGGACG GCGGAGACTG CAGCAAGGGC AAGCTGGAAG
            241 CCCCAGCTGT CAGGGAAGAA GTAGGGCTAT GAGGTTGAGA GTTCCTGGCT GACAAAGACA
            301 GCGAGGAGAT GTCCTGTGTT GCCCTGGCTG CCCCATGCTC GGTACCGTGC TGATACCTCA
            361 TCAGTTGCCC CTCTGTTCTC CCTGGCTGGT TCAGGCGATG CTCACTATAG AAGTGCCCTG
            421 GCTCTTGGGA CTTACACACT ACAGACTGGG ATGGCACGCC CTTGAAAGGG TGTTCGGATC
            481 CGGGACCTCG GTGTTCCATG CCCTTCAGAC TATGTTGGCT TGGAGGTGGC CCTTGGGCCT
            541 TGTAGAATTC ACTGGAACTG GTGGCATTCT TGTAGAGATC ATTGGGTTGT GGTGGGGAGA
            601 GAGGAGCACT GGTAACAGGT GGGTGGGCTT TAACTCCACT AGTAGCCAAT GACATCTGCA
            661 TCAGTCTTTC ACTCAAGGAT CGGACATGCC CTTGCTTAAG ATCTCGAAGG CCTTCATCTT
            721 GGTGCATCTT CCCGGGCGTG AGGCGCTGGA CTGACGGGCG TCCCTCGGTT CTCATCTTCT
            781 GGTTGGTGAC TCCTGTGACA TAGAAAGCAG CCCCAACACT AGCATGCTGC TGGCCCCTGA
            841 AGTACTGGGA CTGGACCTTG GCCTCTTCAT GGGTTGCGAA TTCTTCGTTA TTCTGCATTC
            901 GAGGGGACAG CTGCTTCTCC ATGACGCCGT TTTCTGACTG GATTTCCTGA CCCTGGGGCT
            961 CTTGGCGAGC AGGTGTGCCA CAAGCTGCTG CTGGTGTGAT CCTGGAACTC AATACATCAT
           1021 TCTGAGGGCC TTGATCCCAC TGCCCGTGAA AATGGAGAGC TATCCTGTGC TTGCTGTGTA
           1081 TGGCAGCCAG ACTGCCAATC CTCACTGGGA TGCATAGCGA AGCTGCTCTG GAGCCAGTCG
          1141 GTTGCAGTAC GTCGATTCTC CTGCTGATCG ATCGCACTTT CTGTGAAATT CGA
          //
          Primer: GNP_primer#10
          Sequence file: RDB12206_A5Hpc.seq check
          >12206_12206_A5Hp_GNP_primer#10_F05_17.ab1
              1 TTTTTTGTGC CATTCATTTA AAAAGATAAC GTTTACAGGT TCTTTGGTGC CCATAGTCGA
             61 GCGGCCAGCT TTAGAGCACA TTCTGCTTAA CCTATCTTTT TGAGGTACTC CCTAAGTATT
            121 CCAAAATAGA TTGGAATTCG TTTCTATGGT ATTACTTATG TACTACCAGA GTCCAATAAA
            181 AAGTCCCTTT AGAAAAATCA TCCCCACCTC CCCACCCTAT CTTCACCCCA GATTAAACAA
            241 ATGAAAGGGA AAAATTATTA AAAAGAACTC TTGCTGATAA CGCCAGCTCT TGATTGTGCT
            301 GTTTAGATGA GATATTCCAC CATCTCTGCA TCAGGCTCTT GCCCCAGGAT CTGAATGGGT
            361 GTCTTTCTTT CCAGAGTGCT GGAGTGGAAA GCAGGGGCAT CTGGCTTATC TGGGTTGCAC
            421 ATCAGATTTG GTGTAGACAA GCGGCGTGGT CCGGAACTGG CACCTGGATT TGCAGGACCC
            481 TCAGTTTGAA CCAAAGCTGG AGTTGGAGTT GGAACTGGAC TAGGAGGTTC AGTCGGAGCT
            541 GGAGTTGATG CCTGAGTCGG AGTCGGAGCA GAAGCCTGAG CCGTTGCTGG AGCCGGAATC
            601 GAAGCTGGAG ATGGAACCGG AGCCGAAGTA GCTGGAGCAA CCTGAATAGC AGTAGTGGCA
            661 GCAGCAGCAG CAGCAGTGAT AGCAGCAGTA GTAGCTGCAG CAACAGCAGC AGTAGCAGTG
            721 GCAGCAGTTG GAGAAACAGT AGCAGGAGTA AGAGATGCAG CAGCTGGAAT CTGAACAGCA
            781 GCAGCTGGAG AGACAGGAGC AGCTGGAGGC ACTACAGCAG CTGGAGCGAC AGGAGCAGCT
            841 GGAGAGATAG AATTAGCTGC AGAGACAGAA GTAGCTGCAG AGACAGAAGT AGCTGGAGCG
            901 GCAGCAGCGG CAAGAGCAGC AGCGTTTGCT GGAGATGGGG TGCGGCAGCA GCAGCTGGAG
            961 CATCAACAGC AACTTGTAGC AGCAGCTACC ATGATAGTGG TGTTTGTGGC ACCATGCCAT
          1021 GTTGGTGCAC CAGTTGCATG ATGGCAACCA CCAGGTGCGC AAGCAC
          //
          Primer: BGH_rev
          Sequence file: RDB12206_A5Hpd.seq check
          >12206_12206_A5Hp_BGH_rev_H08_23.ab1
              1 CATACTCATG GTGATGGTGA TGATGACCGG TATGCATATT CAGATCCTCT TCTGAGATGA
             61 GTTTTTGTTC GAAGGGCCCT CTAGACTCGA GGTACGCCAC AGCGTATGAT GCGTATTTTT
            121 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
            181 TTTTTTCCCT TTCCCAAATT TTTTTTTGGG CCCTTTCATT TAAAAAAAAA AACTTTAAGG
            241 GGTTTTTGGG GCCCCAAAGC CAAGGGGCCC TTTTTAAAAC CCTTTCGGCT AACCCCTTTT
            301 TTTGGGGGAC CCCCCAAAAA TCCCAAAAAA AAAGGGAAAT CCTTTTCTGG GGATTTATTT
            361 AGGACCCCCC AAACCCCAAA AAAAACCCCC TTAAAAAAAA CCCCCCCCCC CCCCCCCCCC
            421 TTTTTCCCCC CCAAATTAAA CAAGGGGGGG GGAAAATTTT TTAAAAAAAC CCTTGGGGAA
            481 ACCCCCCCCC TTGGTTGGGG GTTTTAAAAA AAAATTCCCC CCCCCCCGGC ACAGGGCTTT
            541 GGCCAAAATT TAAGGGGGGT TTTTTTTTTC AAAGGGGGGG AGGGAAGGGG GGCTTGGCAT
            601 CCTGGTTTCA CTCAAATTGG GGAAAACGGT GTCGAACGGC CCTGATTGAG ACCTCTTTAA
            661 CAACTGAGTG AGTTGAACTG ACCAGAGTCA TCGACCGGAT TATGCCTATC CGAATCGACA
            721 AATCTTAGCC TGTGTGAACG ATCACTGGAT GAACGACCAA GTTCGACAAC TGAATCATAT
            781 GGCCCACACC CAGTTAGCAC GTAGTGCACA CGCGATATTG CCGTTGAACT CAAGATAAGT
          841 CGCGCTACGA CACGCTGAAC GGAGCGTGGA ACTACTGACC GACTGATGAT
          //
          Primer: SV40pro_F
          Sequence file: RDB12206_A5Hpe.seq check
          >12206_12206_A5Hp_SV40pro_F_D09_12.ab1
              1 CACTGTCCTG TACATCAGAG TAGTGAGGAG GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA
             61 AGCTCCCGGG AGCTTGTATA TCCATTTTCG GATCTGATCA AGAGACAGGA TGAGGATCGT
            121 TTCGCATGAT TGAACAAGAT GGATTGCACG CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC
            181 TATTCGGCTA TGACTGGGCA CAACAGACAA TCGGCTGCTC TGATGCCGCC GTGTTCCGGC
            241 TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG TCAAGACCGA CCTGTCCGGT GCCCTGAATG
            301 AACTGCAGGA CGAGGCAGCG CGGCTATCGT GGCTGGCCAC GACGGGCGTT CCTTGCGCAG
            361 CTGTGCTCGA CGTTGTCACT GAAGCGGGAA GGGACTGGCT GCTATTGGGC GAAGTGCCGG
            421 GGCAGGATCT CCTGTCATCT CACCTTGCTC CTGCCGAGAA AGTATCCATC ATGGCTGATG
            481 CAATGCGGCG GCTGCATACG CTTGATCCGG CTACCTGCCC ATTCGACCAC CAAGCGAAAC
            541 ATCGCATCGA GCGAGCACGT ACTCGGATGG AAGCCGGTCT TGTCGATCAG GATGATCTGG
            601 ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG AACTGTTCGC CAGGCTCAAG GCGCGCATGC
            661 CCGACGGCGA GGATCTCGTC GTGACCCATG GCGATGCCTG CTTGCCGAAT ATCATGGTGG
            721 AAAATGGCCG CTTTTCTGGA TTCATCGACT GTGGCCGGCT GGGTGTGGCG GACCGCTATC
            781 AGGACATAGC GTTGGCTACC CGTGATATTG CTGAAGAGCT TGGCGGCGAA TGGGCTGACC
            841 GCTTCCTCGT GCTTTACGGT ATCGCCGCTC CCGATTCGCA GCGCATCGCC TTCTATCGCC
            901 TTCTTGACGA GTTCTTCTGA GCGGGACTCT GGGGTTCGCG AAATGACCGA CCAAGCGACG
            961 CCCAACCTGC CATCACGAGA TTTCGATTTC ACCGCGCTTT CTATGAAGGT TGGGCTTCGG
           1021 ATCGTTTCGG GACGGCGGCT GATGATCCTC AGCGCGGGAT CTCATGCTGA AGTCCTCGCC
           1081 ACCCACCTGG TTATTGCCAG CTATATGTAC AATTAAGCCA TAGCATCACA ATTTCAACAA
          1141 ATAAAGGCCA GT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl