RDB12205 - HA-Amot130-delta CC/pcDNA3.1-pA83

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HA-Amot130-delta CC/pcDNA3.1-pA83

Expression clone of mouse Amot (p130), deletion of CC domain

Catalog number	RDB12205
Resource name	HA-Amot130-delta CC/pcDNA3.1-pA83
Clone info.	Expression clone of mouse Amot (p130), deletion of coiled-coil (CC) domain. HA-tag.
Comment	See Fig. 4A of Hirate et al., Curr. Biol. 23: 1181-1194 (2013). The expression vector has a stretch of poly(A).
Vector backbone	pcDNA3.1-pA83 (Plasmid)
Size of vector backbone	5.9 kb
Selectable markers	Amp^r
Gene/insert name	Mouse Amot cDNA
Depositor\|Developer	Sasaki, Hiroshi \|

Other clones in our bank

mouse Amot

External Database
Mouse Amot

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB12205	HA-Amot130-delta CC/pcDNA3.1-pA83	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original	Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB12205_A5Hpp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward Sequence file: RDB12205_A5Hpa.seq
>12205_12205_A5Hp_CMV-Forward_F11_17.ab1 1 TTGTTTCACT CCGCCCATTG ACGCAAATGG GCGGTAGGCG TGTACGGTGG GAGGTCTATA 61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA 121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGTTAAGCTA GCTTGCCGCC ACCATGTACC 181 CATACGACGT TCCAGATTAC GCTGGATCCA GAAGTTCTGA CGATCAGCCA AGTGGAGGAA 241 CGACGGTACT GCAACGGCTG CTTCAAGAGC AGCTTCGCTA TGGCAATCCC AGTGAGAATC 301 GCAGTCTGCT TGCCATACAC CAGCAAGCCA CAGGGAATAG CTCTCCATTT TCCACGGGCA 361 GTGGGAACCA AGGCCCTCAG AATGATGTAT TGAGTTCCCA GGATCACCAC CAGCAGCAGC 421 TTGTGGCACA CCCTGCTCGC CAAGAGCCCC AGGGTCAGGA AATCCAGTCA GAAAACGGCG 481 TCATGGAGAA GCAGCTGTCC CCTCGAATGC AGAATAACGA AGAACTCCCA ACCTATGAAG 541 AGGCCAAGGT CCAGTCCCAG TACTTCAGGG GCCAGCAGCA TGCTAGTGTT GGGGCTGCTT 601 TCTATGTCAC AGGAGTCACC AACCAGAAGA TGAGAACCGA GGGACGCCCG TCAGTCCAGC 661 GCCTCACGCC CGGGAAGATG CACCAAGATG AAGGCCTTCG AGATCTTAAG CAAGGGCATG 721 TCCGATCCTT GAGTGAAAGA CTGATGCAGA TGTCATTGGC TACTAGTGGA GTTAAAGCCC 781 ACCCACCTGT TACCAGTGCT CCTCTCTCCC CACCACAACC CAATGATCTC TACAAGAATG 841 CCACCAGTTC CAGTGAATTC TACAAGGCCC AAGGGCCACC TCCAAGCCAA CATAGTCTGA 901 AAGGGCATGG AACACCGAGG TCCCCCGCCA GAATACCCTT TCAAGGGCGT GCCATCCCAG 961 TCTGTAGTGT GTAAGTCCCA AGAGCCAGGG CACTTCTATA GTGAGCATCG CCTGAACCAG 1021 CCAGGGAGAA CAGAGGGGCA ACTGATGAGT ATCAGCACCC CCCCTGAGTA TGGGGCAGCC 1081 AGGGCAACAC AGAACATCTC CTTCGCTGTC TTTTGTCAGC AGGAAACTCT CACTTCATAG 1141 CCCTTACTCA TCCTGAGGCT TGAAGGACCT TTCTCCCCAG //
Primer: mAmot-M#1 Sequence file: RDB12205_A5Hpb.seq
>12205_12205_A5Hp_mAmot-M#1_H08_23.ab1 1 GGGGGAAGCT ACTCAGCTAT GCCTCGGGCT CAGCAGTCTG CTTCTTATCA GCCCATGCCA 61 GCTGACCCTT TTGCTATCAG ACACTTTGCT CTGGATGCTG CTGCAACTGT GGCTGCACAG 121 AGAGACACAA CAGTCATTAG CCACTCTCCT AACACCAGCT ATGACACAGC CTTAGAGGCC 181 CGCATCCAGA AGGAAGAGGA AGAAATCCTG ATGGCCAACA AGCGTTGCTT GGACATGGAA 241 GGAAGGATTA AGACCCTCCA TGCCCAGATT ATTGAAAAGG ATGCCATGAT CAAGGTGCTG 301 CAGCAGCGCT CCAGAAAGGA GCCGAGTAAA ACAGAGCAAC TCTCATCCAT GCGGCCAGCA 361 AAGTCTCTGA TGTCCATTTC CAATGCTGGA TCAGGCCTCC TTGCACACTC CTCTACCTTG 421 ACTGGTGCGC CCATTATGGA GGAGAAGCGT GATGACAAGA GCTGGAAGGG GAGCCTAGGC 481 GTTCTTCTGG GAGGAGATTA CCGTGTTGAG CCGGTCCCAT CCACACCGTC CCCTGTGCCC 541 CCGTCTACTC CCCTTCTCTC AGCTCATTCG AAGACAGGCA GTCGCGATTG CAGCACCCAA 601 ACTGAACGTG GACCGGAATC GACCAAAACC GCAGCTGTCA CTCCCATCTC GGCTCCAATG 661 GCTGGTCCAG TTGCTGCTGC TGCCCCTGCT GCTGCCATCA ATGCAACTGC TGCCACCAAC 721 ACTGCCACTG CTGCCACCAA CACCACTATC ATGGTAGCTG CTGCTCCAGT TGCTGTTGCT 781 GCTCCAGCTG CTGCTGCCGC CACCCCATCT CCAGCAAACG CTGCTGCTCT TGCCGCTGCT 841 GCCGCTCCAG CTACTTCTGT CTCTGCAGCT ACTTCTGTCT CTGCAGCTAA TTCTATCTCT 901 CCAGCTGCTC CTGTCGCTCC AGCTGCTGTA GTGCCTCCAG CTGCTCCTGT CTCTCCAGCT 961 GCTGCTGTTC AGATTCCAGC TGCTGCATCT CTTACTCCTG CTACTGTTTC TCCAACTGCT 1021 GCCACTGCTA CTGCTGCTGT GCTGCAGCTA CTACTGCTGC TATCACTGCT GCTGCTGCTG 1081 CTGCACTACT GCTATTCACT GCTCGAGCTA CTCGCTCGAT CATCTCCAGC CTTCGATTCG 1141 GGCTCAGGCA ACGGGCCTTC AAGCT //
Primer: GNP_primer#10 Sequence file: RDB12205_A5Hpc.seq
>12205_12205_A5Hp_GNP_primer#10_B05_05.ab1 1 CTTATTTGTG CCATTCATTT AAAAGATAAC GTTTACAGGT TCTTTGGTGC CCATAGTCGA 61 GCGGCCAGCT TTAGAGCACA TTCTGCTTAA CCTATCTTTT TGAGGTACTC CCTAAGTATT 121 CCAAAATAGA TTGGAATTCG TTTCTATGGT ATTACTTATG TACTACCAGA GTCCAATAAA 181 AAGTCCCTTT AGAAAAATCA TCCCCACCTC CCCACCCTAT CTTCACCCCA GATTAAACAA 241 ATGAAAGGGA AAAATTATTA AAAAGAACTC TTGCTGATAA CGCCAGCTCT TGATTGTGCT 301 GTTTAGATGA GATATTCCAC CATCTCTGCA TCAGGCTCTT GCCCCAGGAT CTGAATGGGT 361 GTCTTTCTTT CCAGAGTGCT GGAGTGGAAA GCAGGGGCAT CTGGCTTATC TGGGTTGCAC 421 ATCAGATTTG GTGTAGACAA GCGGCGTGGT CCGGAACTGG CACCTGGATT TGCAGGACCC 481 TCAGTTTGAA CCAAAGCTGG AGTTGGAGTT GGAACTGGAC TAGGAGGTTC AGTCGGAGCT 541 GGAGTTGATG CCTGAGTCGG AGTCGGAGCA GAAGCCTGAG CCGTTGCTGG AGCCGGAATC 601 GAAGCTGGAG ATGGAACCGG AGCCGAAGTA GCTGGAGCAA CCTGAATAGC AGTAGTGGCA 661 GCAGCAGCAG CAGCAGTGAT AGCAGCAGTA GTAGCTGCAG CAACAGCAGC AGTAGCAGTG 721 GCAGCAGTTG GAGAAACAGT AGCAGGAGTA AGAGATGCAG CAGCTGGAAT CTGAACAGCA 781 GCAGCTGGAG AGACAGGAGC AGCTGGAGGC ACTACAGCAG CTGGAGCGAC AGGAGCAGCT 841 GGAGAGATAG AATTAGCTGC AGAGACAGAA GTAGCTGCAG AGACAGAAGT AGCTGGAGCG 901 GCAGCAGCGG CAAGAGCAGC AGCGTTTGCT GGAGATGGGG TGGCGGCAGC AGCAGCTGGA 961 GCAGCAACAG CAACTGGAGC AGCAGCTACC ATGATAGTGG TGTTGGTGGC AGCAGTGGCA 1021 GTGTTGGTGG CAGCAGTTGC ATTGATGGCA GCAGCAGGGG CAGCAGCAGC AACTGGACCA 1081 GCCATTGAGC CGAGATGGGG AGTGACAGCT GCGGATTTGG TCGATTCCGG TTCCACGTTT 1141 CAGTGGGTGC CTGCAATCGC GACTGCCGGC TTCGATGACT GAAGAAGAGG GAATTAAACG 1201 GGGGTCAG //
Primer: BGH_rev Sequence file: RDB12205_A5Hpd.seq
>12205_12205_A5Hp_BGH_rev_C05_08.ab1 1 CTCTACTCAT GGTGATGGTG ATGATGACCG GTATGCATAT TCAGATCCTC TTCTGAGATG 61 AGTTTTTGTT CGAAGGGCCC TCTAGACTCG AGGTACGCCA CAGCGTATGA TGCGTATTTT 121 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT 181 TTTTTTCCTT CCCCAAATTT TTTTTGGGCC CCTTTCATTT AAAAAAAAAA ACTTTTAGGG 241 GTTTTTGGGG CCCCAAACCC AAGGGCCCCT TTTAAAAACC CTTTCGGTTA ACCCTTTTTT 301 TTGGGGGCCC CCCCAAATTT TCCCAAAAAA AATGGGATTT TTTTTCTGGG GTTTTTTTTT 361 GGCCCCCCCA AACCCCAAAA AAAACCCCTT TAAAAAAAAC CCCCCCCCCC CCCCCCCCCT 421 TTTTCCCCCC CAAATTAACA AAGGGGGGGG AAAAATTTTT TAAAAAAACC TTTTGTTAAA 481 ACCCCCCCCC CTGGTTGGGG TGGTTAAAAA AAAAATTCCC CCCCCCCCCG CACCGGGCTT 541 TCCCCCCAAA ATTAAAGGGG GGTTTTTTTT TCCCAAAGGG GGGGGGGGAA AAAGGGGGGC 601 CCCGGGTTTT TGGGGGTGCC CCCCAAAATT GGGGGAACCC CAGGGGGGGG GCCCGGAACG 661 GGCCCGGTTT TTCCGGGCCT TCTTGTGGGC CCACCTGAAT TGAGTTTGGA CTGATAGAGC 721 TTCCCGGAGC GGATTAGGGC GGGACCGAAC CGAACAACCG GACGTTGCGG ACCGATCACT 781 GGATGACCGG ACAATTCTGG ACACTGAATC AATGCCCCCC CCCAGTACCC ATACTGACAC 841 CCCATACTGC AGTGCATCTC AGATTAATGC CGCTGATCGA CACCTTGAAC CGACCTTGGA 901 CTCATCGTTG ACAGGATCTG ACTATTCCGA CAAGTGCGCA TATCCAGTCA ACGGAGCCGT 961 G //
Primer: SV40pro_F Sequence file: RDB12205_A5Hpe.seq
>12205_12205_A5Hp_SV40pro_F_D05_11.ab1 1 CCCTGTCCTT GGCATTCAGA GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA 61 AAGCTCCCGG GAGCTTGTAT ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG 121 TTTCGCATGA TTGAACAAGA TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG 181 CTATTCGGCT ATGACTGGGC ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG 241 CTGTCAGCGC AGGGGCGCCC GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT 301 GAACTGCAGG ACGAGGCAGC GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA 361 GCTGTGCTCG ACGTTGTCAC TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG 421 GGGCAGGATC TCCTGTCATC TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT 481 GCAATGCGGC GGCTGCATAC GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA 541 CATCGCATCG AGCGAGCACG TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG 601 GACGAAGAGC ATCAGGGGCT CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG 661 CCCGACGGCG AGGATCTCGT CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG 721 GAAAATGGCC GCTTTTCTGG ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT 781 CAGGACATAG CGTTGGCTAC CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC 841 CGCTTCCTCG TGCTTTACGG TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC 901 CTTCTTGACG AGTTCTTCTG AGCGGGACTC TGGGGTTCGC GAAATGACCG ACCAAGCGAC 961 GCCCAACCTG CCATCACGAG ATTTCGATTC CACCGCCGCC TTCTATGAAG GTTGGGCTTC 1021 GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC GCGGGATCTC ATGCTGAGTT 1081 CTTCGCCCAC CCCCAACTGT TTATTGCAGC TTATATGGTT ACAAATTAGG CCATAGCATC 1141 ACAATTCACA ATAAGCATTT TTTTCCACCT GCCATTTCTC TATAGTTGTG TTGGATG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Hirate, Y., Polarity-dependent distribution of angiomotin localizes Hippo signaling in preimplantation embryos. Current Biology 23, 1181-1194 (2013). PMID 23791731.

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl