Please review the QC test results indicated by icon below as well as clone information before placing your order.

HA-Amot130/pcDNA3.1-pA83

Expression clone of mouse Amot (p130)

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Hirate, Y., et al., Curr. Biol. 23, 1181-1194 (2013))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12203_A5Hpp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: HA tag, insert 5' Sequence file: RDB12203_A5Hpa.seq
>12203_12203_A5Hp_CMV-Forward_A04_01.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGCNNNGG GCGGTAGGCG TGTNCNGNGG GAGGTCTATA    61 TAAGCAGAGC TCTCTGGCTA ACTAGAGAAC CCACTGCTTA CTGGCTTATC GAAATTAATA   121 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGTTAANCTN NCTTGCCGCC ACCATGTACC   181 CATACGACGT TCCAGATTAC GCTGGATCCA GAAGTTCTGA CGATCAGCCA AGTGGAGGAA   241 CGACGGTACT GCAACGGCTG CTTCAAGAGC AGCTTCGCTA TGGCAATCCC AGTGAGAATC   301 GCAGTCTGCT TGCCATACAC CAGCAAGCCA CAGGGAATAG CTCTCCATTT TCCACGGGCA   361 GTGGGAACCA AGGCCCTCAG AATGATGTAT TGAGTTCCCA GGATCACCAC CAGCAGCAGC   421 TTGTGGCACA CCCTGCTCGC CAAGAGCCCC AGGGTCAGGA AATCCAGTCA GAAAACGGCG   481 TCATGGAGAA GCAGCTGTCC CCTCGAATGC AGAATAACGA AGAACTCCCA ACCTATGAAG   541 AGGCCAAGGT CCAGTCCCAG TACTTCAGGG GCCAGCAGCA TGCTAGTGTT GGGGCTGCTT   601 TCTATGTCAC AGGAGTCACC AACCAGAAGA TGAGAACCGA GGGACGCCCG TCAGTCCAGC   661 GCCTCACGCN CGGGAAGATG CACNAAGATG AAGGNCTTCG AGATCTTAAG CAAGGGCATG   721 TCCGATCCTT GAGTGAAAGA CTGATGCAGA TGTCATTGGC TACTAGTGGA GTTAAAGCCC   781 ACCNNCCTGT TANCAGTGCT CCTCTCTCCN CACCACAACC CNATGATCTC TACAAGAATG   841 CCACCAGTTN NNGTGAATTC TACAAGGNCN NAGGGCCANN NNNNAGNNNA CNTAGNCTGA   901 AGGGNNTGGA ANNNNNAGGN CCCNNGCCAG ANTANCNTTT CAAGGGCGTG NNNTCNNNNN   961 NNNNAGTGTG NNAGTCCNNN NANNNNGGNN NNNNCNNNAN TGANCNTNNN CNTNNNNNNG 1021 NCNNNNNNNN NN //
Primer: mAmot-M#1 Sequence file: RDB12203_A5Hpb.seq
>12203_12203_A5Hp_mAmot-M#1_E08_14.ab1     1 AGGGGAGAGC TACTCAGCTA TGCCTCGGGC TCAGCAGTCT GCTTCTTATC AGCCCATGCC    61 AGCTGACCCT TTTGCTATCG TCTCTAGAGC TCAGCAGATG GTTGAGATCC TGTCTGATGA   121 GAATCGCAAC TTGCGGCAGG AGTTGGATGG ATGCTATGAG AAGGTGGCGA GACTGCAGAA   181 GGTGGAGACA GAAATCCAAC GGGTCTCAGA GGCTTATGAG AACTTGGTGA AGTCATCTTC   241 CAAAAGAGAG GCTCTGGAGA AAGCCATGAG GAACAAGCTG GAGGGCGAGA TTAGAAGGAT   301 GCATGACTTC AACAGAGATC TGAGAGACCG TCTAGAGACT GCCAACAAGC AGCTGGCAGA   361 GAAGGAGTAC GAGGGGTCCG AGGACACCAG GAAGACCATC TCGCAGCTCT TTGCCAAACA   421 TAAAGAAAAC CAGCGGGAGA AGGAGAAGCT GGAAGCTGAG CTGGCTACTG CCCGTTCTAC   481 CAATGAAGAC CAAAGGCGAC ACATTGAAAT CCGAGACCAG GCATTGAGCA ATGCCCAGGC   541 GAAGGTGGTG AAGCTGGAAG AAGAGCTGAA AAAGAAGCAA GTGTACGTAG ATAAGGTGGA   601 GAAGATGCAG CAGGCCCTTG TGCAGCTCCA GGCCGCCTGT GAGAAGCGTG AGCAGCTGGA   661 ACACCGGCTC CGGACACGTT TGGAGAGGGA ACTGGAATCC CTTAGAATCC AGCAGCGCCA   721 GGGCAACTCC CAGCCCACTA ATGCTTCAGA ATACAATGCC GCTGCACTGA TGGAGCTCCT   781 CCGGGAGAAG GAAGAGAGAA TTCTGGCCCT GGAAGCAGAT ATGACCAAGT GGGAGCAAAA   841 GTATTTGGAA GAGAATGTGA TGAGACACTT TGCTCTGGAT GCTGCTGCAA CTGTGGCTGC   901 ACAGAGAGAC ACAACAGTCA TTAGCCACTC TCCTAACACC AGCTATGACA CAGCCCTTAG   961 AGGCCCGCAT CCAGAAGGAA GAGGAGAAAT CCTGATGGGC CAACAAGCGT TGCTTGACAT  1021 GGAAGGAGGA TTAAGACCTC ATGCCAGATA TGAAAAGATG CAATGATCAG TGCTGCAGCA  1081 GCGCTCCAGG AAAGAAGCGA GTTAAACCAG AGCAACTTCG ATCCAATGCG CAGTCAGTCC 1141 TGAATGTTCC GATCTTTTTT CA //
Primer: mAmot-M#1 Region: insert mid Sequence file: RDB12203_A5Hpc.seq
>12203_12203_A5Hp_mAmot-M#1_E08_14.ab1     1 NNNNNNNNNN NNNNNNGCNN TGCCTCNGGC TNNGCAGTCT GCTTCTTATC NGCCCNTGCC    61 NGCTGACCCT TTTGCTATCG TCTCTAGAGC TCAGCAGATG GTTGAGATCC TGTCTGATGA   121 GAATCGCAAC TTGCGGCAGG AGTTGGATGG ATGCTATGAG AAGGTGGCGA GACTGCAGAA   181 GGTGGAGACA GAAATCCAAC GGGTCTCAGA GGCTTATGAG AACTTGGTGA AGTCATCTTC   241 CAAAAGAGAG GCTCTGGAGA AAGCCATGAG GAACAAGCTG GAGGGCGAGA TTAGAAGGAT   301 GCATGACTTC AACAGAGATC TGAGAGACCG TCTAGAGACT GCCAACAAGC AGCTGGCAGA   361 GAAGGAGTAC GAGGGGTCCG AGGACACCAG GAAGACCATC TCGCAGCTCT TTGCCAAACA   421 TAAAGAAAAC CAGCGGGAGA AGGAGAAGCT GGAAGCTGAG CTGGCTACTG CCCGTTCTAC   481 CAATGAAGAC CAAAGGCGAC ACATTGAAAT CCGAGACCAG GCATTGAGCA ATGCCCAGGC   541 GAAGGTGGTG AAGCTGGAAG AAGAGCTGAA AAAGAAGCAA GTGTACGTAG ATAAGGTGGA   601 GAAGATGCAG CAGGCCCTTG TGCAGCTCCA GGCCGCCTGT GAGAAGCGTG AGCAGCTGGA   661 ACACCGGCTC CGGACACGTT TGGAGAGGGA ACTGGAATCC CTTAGAATCC AGCAGCGCCA   721 GGGCAACTCC CAGCCCACTA ATGCTTCAGA ATACAATGNC GCTGCACTGA TGGAGCTCCT   781 CCGGGAGAAN GAAGAGAGAA TTCTGGCCNT GGAAGCAGAT ATGACCNAGT GGGANCAAAA   841 GTATTTGNNA GAGANTGTGA TGAGANACTT TGCTCTGGNN GCTGCTGCAN NNGNNGCNGC   901 ACAGAGAGAC ACNACAGNCA NNANCNNNTN NNNTAACANN AGNNATGNNN CAGNCNNTAG 961 NNNNCNNNNT NCNGNNNNNN NNNN //
Primer: mAmot-M#2 Region: insert mid Sequence file: RDB12203_A5Hpd.seq
>12203_12203_A5Hp_mAmot-M#2_H10_22.ab1     1 NNNNNNNNNN NNNNNNNCNN NNNNNNNGNN TAGCTGANTA GGCTTCTCCT GGCTGCTGCT    61 GCGCTGAAGT CAGGCTGGCC TGGGCGTGAC GGTAGTGGTC CCCCTGATGA CTCTGACTCG   121 AAAGAAGATG CTGCTGATGC CTTGACAGGT GAGCAGAGTG GTCACCTTGG TTCGGGACCA   181 GGTGTTGACC AGGAGGCAAT CTAGTGGACG GCGGAGACTG CAGCAAGGGC AAGCTGGAAG   241 CCCCAGCTGT CAGGGAAGAA GTAGGGCTAT GAGGTTGAGA GTTCCTGGCT GACAAAGACA   301 GCGAGGAGAT GTCCTGTGTT GCCCTGGCTG CCCCATACTC AGGGGGGTGC TGATACCTCA   361 TCAGTTGCCC CTCTGTTCTC CCTGGCTGGT TCAGGCGATG CTCACTATAG AAGTGCCCTG   421 GCTCTTGGGA CTTACACACT ACAGACTGGG ATGGCACGCC CTTGAAAGGG TATTCTGGCG   481 GGGGACCTCG GTGTTCCATG CCCTTCAGAC TATGTTGGCT TGGAGGTGGC CCTTGGGCCT   541 TGTAGAATTC ACTGGAACTG GTGGCATTCT TGTAGAGATC ATTGGGTTGT GGTGGGGAGA   601 GAGGAGCACT GGTAACAGGT GGGTGGGCTT TAACTCCACT AGTAGCCAAT GACATCTGCA   661 TCAGTCTTTC ACTCAAGGAT CGGACATGCC CTTGCTTAAG ATCTCGAAGG CCTTCATCTT   721 GGTGCATCTT CCCGGGCGTG AGGCGCTGGA CTGACGGGCG TCCCTCGGTT CTCATCTTCT   781 GGTTGGTGAC TCCTGTGACA TANAAAGCAG CCCCNACACT AGCATGCTGC TGGNCCCTGA   841 AGTACTGNGA CTGGACNTTG GCCTCTTCAT ANGTTGGGAN TTCTTCGTTA TTCTGCATTC   901 NAGGGGACAG CTGCTTCTNC NTGANGCCGT TTTCTGANTG GNTTTCCTGA NCCNNGGGCT   961 CNTNNNNANC NGGNNGNGNN NCAAGCTGCT GNTNGNNNNG ATNCNGGNNN NTCNNNACNT 1021 CNTTNNGNNN NNNNNNN //
Primer: BGH_rev Sequence file: RDB12203_A5Hpe.seq
>12203_12203_A5Hp_BGHrev_G08_20.ab1     1 CACTACTCAT GGTGATGGTG ATGATGACCG GTATGCATAT TCAGATCCTC TTCTGAGATG    61 AGTTTTTGTT CGAAGGGCCC TCTAGACTCG AGGTACGCCA CAGCGTATGA TGCGTATTTT   121 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT   181 TTTTTTTTTC CTTCCCCAAC TTTTTTTTGG GCCCCTTCCT TTTAAAAAAA AAACCTTTTC   241 GGGGTTTTTG GGGCCCCAAA CCCAGGGGCC CCTTTTAAAA CCCCTTTCGC TTAACCCTTT   301 TTTTTGGGGG CCCCCCCAAA ATTCCCAAAA AAAAAGGGAA ACCCTTTCCG GGGGTTTTCC   361 TTGGGCCCCC CCAAACCCCA AAAAAACCCC CTTTAAAAAA AATCCCCCCC CCCCCCCCCC   421 CCTTTTTCCC CCCCAATTAA ACAAAGGGAG GGGAAAAATT TTTAAAAAAA ACCCTTTGGA   481 AAACCCCCCC CCTTGATTGG GGGTTTTAAA AAAAAATTCC CCCTTCCCGG AAAAGGGTTT   541 TGCCCCAAAA TTTGGAGGGG GGTTTCCTTT CAAAAGGGGG GGGGGGAACC GGGGGCTTCC   601 GTTATACCTG TTTTGCCTAC ATATGGTGAA ACACCGCGGT CCGGGAGGGG CCCGGATTTG   661 AGGACCTCGT TTGACCAAGC TGAATTGATT GAACGGACTA GAGTCCTCGA CTGATTATCC   721 TGATCGATCG AACAACCGGG CTTGGGGGCC GGATCAGCTG GATGACCGGA CCAAGTTCGA   781 CACTGGATCC CATGGCCACC CCCCCAGTTA GCCAATAACT CGCACAGCAG ATACTTGTCC   841 GTGGAACTTC AAGGTTTAAT TTCCGCGTAT CGACGGCGTG ACGGACCTTG ACATGTGACG 901 ATCCTGTAAT ATTCGAAAAA //
Primer: SV40pro_F Sequence file: RDB12203_A5Hpf.seq
>12203_12203_A5Hp_SV40pro_F_D04_10.ab1     1 GCGCTTGGTC ATTAACATTC AGAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC    61 AAAAAGCTCC CGGGAGCTTG TATATCCAAT TTCGGATCTG ATCAAGAGAC AGGATGAGGA   121 TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT CTCCGGCCGC TTGGGTGGAG   181 AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT GCTCTGATGC CGCCGTGTTC   241 CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA CCGACCTGTC CGGTGCCCTG   301 AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG CCACGACGGG CGTTCCTTGC   361 GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT GGCTGCTATT GGGCGAAGTG   421 CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG AGAAAGTATC CATCATGGCT   481 GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT GCCCATTCGA CCACCAAGCG   541 AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG GTCTTGTCGA TCAGGATGAT   601 CTGGACGAAG AGCATCAGGG GCTCGCGCCA GCCGAACTGT TCGCCAGGCT CAAGGCGCGC   661 ATGCCCGACG GCGAGGATCT CGTCGTGACC CATGGCGATG CCTGCTTGCC GAATATCATG   721 GTGGAAAATG GCCGCTTTTC TGGATTCATC GACTGTGGCC GGCTGGGTGT GGCGGACCGC   781 TATCAGGACA TAGCGTTGGC TACCCGTGAT ATTGCTGAAG AGCTTGGCGG CGAATGGGCT   841 GACCGCTTCC TCGTGCTTTA CGGTATCGCC GCTCCCGATT CGCAGCGCAT CGCCTTCTAT   901 CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA CTCTGGGGGT TCGCGAAATG ACCGACCAAG   961 CGACGCCCAA CCCTGCCATC ACGAGATTTC GATTCCCACC GCCGGCCTTC TATGAAAGGT  1021 TGGGCGTCGG AAATCGTTTT CCGGGGACGC CGGGCTGGAA TGGATTCGTC CAGCGACGGG 1081 GGAATCTCAA TGGCGTG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl