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pMYs-HA-Yap1 S112A-IRES-EGFP

Retroviral vector of mouse Yap1, S112A mutant

Catalog number RDB12181
Resource name pMYs-HA-Yap1 S112A-IRES-EGFP
Clone info. Retroviral vector of mouse Yap1, S112A mutation in Tead BD. cDNA fragment 210 to 1,625 nt of NM_009534.3 (CDS, 207..1625) is cloned. T to G substitution at 540 nt and C to A substitution at 542 nt (Ser to Ala at 112th aa, introduced mutation).
Comment Transfection can be confirmed by GFP. See Fig. 3A of Ota and Sasaki, Development 135: 4059-4069 (2008). Known differences: G to C substitution at 212 nt (Glu to Asp at 2nd aa). C to A substitution at 477 nt (Arg at 91st aa, synonymous) and XhoI site insertion at the position.
Vector backbone pMYs-IRES-EGFP (Plasmid)
Size of vector backbone 6.2 kb
Selectable markers Amp^r
Gene/insert name Mouse Yap1 cDNA
Depositor|Developer Sasaki, Hiroshi |
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External Database
Mouse Yap1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
          Additional terms and conditions:
          Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Remarks Remember that you will be working with samples containing infections virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。
          付加的提供条件:
          pMXsシリーズベクターについて:利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄博士への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB12181 pMYs-HA-Yap1 S112A-IRES-EGFP DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pMYs-HA-Yap1 S112A-IRES-EGFP was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB12181).

          Reference section:

          Ota, M., Sasaki, H., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12181_A6Ajp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: 5'Retro (pFB-5')
          Sequence file: RDB12181_A6Aja.seq check
          >12181_12181_A6Aj_5'Retro(pFB-5')_E11_14.ab1
              1 TCAGCTTCTG TTTCGCGGAC TGCTAGTTAT TAAGGATCCC AGTGTGGTGG TACGGGAATT
             61 CCTGCAGGGG GCTAGCGAAT TCGATTAATA CGACTCACTA TAGGGAGACC CAAGCTTGCC
            121 GCCACCATGT ACCCATACGA CGTTCCAGAT TACGCTGGTA CCGACCCCGC GCAACAGCCG
            181 CCGCCCCAGC CGGCCCCGCA AGGCCCCGCG CCGCCGTCCG TGTCTCCGGC CGGGACCCCC
            241 GCGGCCCCGC CCGCACCCCC GGCCGGCCAC CAGGTCGTGC ACGTCCGCGG GGACTCGGAG
            301 ACCGACTTGG AGGCGCTCTT CAATGCCGTC ATGAACCCCA AGACGGCCAA CGTGCCTCAG
            361 ACCGTGCCCA TGCGGCTTCG CAAGCTGCCC GACTCCTTCT TCAAGCCGCC TGAGCCCAAG
            421 TCCCACTCGA GACAGGCCAG TACTGATGCA GGTACTGCGG GAGCTCTGAC TCCACAGCAT
            481 GTTCGAGCTC ACGCATCTCC AGCCTCCCTG CAGCTGGGTG CCGTTTCTCC TGGGACACTC
            541 ACAGCCAGTG GCGTTGTCTC TGGCCCTGCC GCTGCCCCTG CAGCTCAGCA TCTCCGGCAG
            601 TCCTCCTTTG AGATCCCTGA TGATGTACCA CTGCCAGCAG GCTGGGAGAT GGCCAAGACA
            661 TCTTCTGGTC AAAGATACTT CTTAAATCAC AACGATCAGA CAACAACATG GCAGGACCCC
            721 CGGAAGGCCA TGCTTTCGCA ACTGAACGTT CCTGCGCCTG CCAGCCCAGC GGTGCCCCAG
            781 ACGCTGATGA ATTCTGCCTC AGGACCTCTT CCTGATGGAT GGGAGCAAGC CATGACTCAG
            841 ATGGAGAAGT TTACTACATA AACCATAAGA ACAAGACCAC ATCCTGGCTG GACCCAAAGG
            901 CTGGACCCTC GTTTTGCCAT GAACCAGAGG ATCACTCAGA GTGCTCCAGT GAAGCAGCCC
            961 CCCACCCTTT GGCTCCCAGA GCCCACAGGG AGGCGTCTGG GGTGGAGGCA GTTCACAGCA
           1021 GCAGCAAATA CAGCTGCAGC AGTTACGATG GAGATGGAGA GACTGCGGTT GGAACCACCA
           1081 GATTATTTCG CAAGACTAGC TCTGCCAGCC AGGTTGCTAC ACTGAGCAGG AATGGGAGGG
          1141 ACTTC
          //
          Primer: IRES-R
          Sequence file: RDB12181_A6Ajb.seq check
          >12181_12181_A6Aj_IRES_R_E02_14.ab1
              1 GCAATGCGGG CTTCGGCAGT AACGTTAGGG GGGGGGGAGG GAGAGGGGGG GGGGGGGCGG
             61 AATTTCGTAG CGGCCGCGGC GCGCCGGCCC TCGAGGGATC CCTCACAGAC TCAGAGTGGC
            121 TCCCTGCAGC TCTATAACCA CGTGAGAAAG CTTTCTTTAT CTAGCTTGGT GGCAGCCAAC
            181 ACAGACTCCA CGTCCAAGAT TTCGGAACTC AGCGCTTCCT GCAGACTGGG CATCAGCTCC
            241 TCCCCTTCTA TGTTCATTGC ATCTCCTTCC AGTGTGCCAA GGTCCACATT TGTCCCAGGG
            301 AGGGCTTCCA GGTAGTCGGG GAAGCGGCTC TGCTGTGACG GCAGGGTGCT TTGGCTGATG
            361 GTGTCTCCTG TATCCATTTC ATCCACACTG TTGAGGAAGT CGTCTGGGGT CCGAGGGATG
            421 CTGTAGCTGC TCATGCTGAG GCCGCTGTCT GTGCTCTCAT CTCGAGAGTG ATAGGTGCCA
            481 CTGTTAAGAA AGGGATCGGA ACTATTGGTT GTCATTGTTC TCAATTCCTG AGACATCCCA
            541 GGAGAAGACA CTGCATTCGG AGTCCCTCCA TCCTGCTCCA GTGTAGGCAA CTGGCTGCGC
            601 AGAGCTAATT CCTGCCGAAA TAATTCCTGT TGTTTCAACC GCAGTCTCTC CTTCTCCATC
            661 TGTAACTGCT GCAGCTGTAT TTGCTGCTGC TGGTTGGGAA CTGCCCTCCA CCCCAGACGC
            721 CTCCCCTGTG GGCTCTGGGG AGGCCAAGGG GTGGGGGCCT GCCTTCACTG GAGCACTCTG
            781 AGTGATCCCT CTGGTTCATG GCAAAACCGA GGGTCCAGCC CTTGGGGTCC CAGCCAGGAA
            841 TGTGGGTCCT TGTTTCTTAT GGTTTTATGG AAATAAACTT TCTCCATCCT GAAGTCGGGG
            901 CTTTGCTCCC CCTCTATCAG AGAAGAGCTC CTAGAAAGGA AAAATTTCTC CGGCTTGGTG
            961 GGGGCCACCT GGGGGTGGTG AGCGCGCCGG GAGACATTTC TACTTTGTGT AAAAAAATGG
           1021 GTGCCTCTCT TGGGGGGGGC GCTCTCGCGC CATGGGATTG CTGTGGCCAT GGTTGGTGTG
           1081 ATGTATTATC AGGATATTAT CTCTTTCTTT GCCCCACAGA GGAATGGTCT CTCTATGGGG
           1141 CCCTATCTCT CTACCCCAAG GCCTCTTGGA CCTGTGGCGC CGAAGGGTGC GGTGTCTATC
          1201 ATCCATCTAT CGACTAC
          //
          Primer: IRES-F
          Sequence file: RDB12181_A6Ajc.seq check
          >12181_12181_A6Aj_IRES-F_E09_15.ab1
              1 GGCAGGCCGT TTCATGTGTT TAGTCGAGGT TAAAAAAACG TCTAGGCCCC CCGAACCACG
             61 GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GGTGAGCAAG
            121 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC
            181 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC
            241 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC
            301 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC
            361 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC
            421 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC
            481 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC
            541 AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG
            601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG
            661 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC
            721 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC
            781 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAAAG CGGCCGGCCG
            841 CCAGCACAGT GGTCGACCGG GCCGCCAGCA CAGTGGTCGA TCGAGGGGGG GGCCCGCGAT
            901 TAGTCCAATT TGTTAAAGAC AGGATATCAG TGGTCCAGGC TCTAGTTTTG ACTCAACAAT
            961 ATCACCAGCT GAAGCCCTAT AGAGTACGAG CCCATAGATA GAATAAAAGA TTTTATTTAG
           1021 TCTCAAGAAA AAGGGGGGGA ATGAAGACCC CAACCTGTAG GTTTGGCCAA GCTAGCTAGT
           1081 AACGGCATTT TGCCAGGCCA TTGGAAATAC ATTAACTGGA GAAATAAGGA GGAAAGTTCC
          1141 AGGAT
          //
          Primer: Amp_R
          Sequence file: RDB12181_A6Ajd.seq check
          >12181_12181_A6Aj_Amp_R_F09_18.ab1
              1 GTCATTTCAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
             61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
            121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT
            181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG
            241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT
            301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA
            361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC
            421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG
            481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC
            541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATTGACTGAG
            601 TCGCCCGGGT ACCCGTATTC CCAATAAAGC CTCTTGCTGT TTGCATCCGA ATCGTGGACT
            661 CGCTGATCCT TGGGAGGGTC TCCTCAGATT GATTGACTGC CCACCTCGGG GGTCTTTCAT
            721 TTGGAGGTTC CACCGAGATT TGGAGACCCC TGCCCAGGGA CCACCGACCC CCCCGCCGGG
            781 AGGTAAGCTG GCCAGCGGTC GTTTCGTGTC TGTCTCTGTC TTTGTGCGTG TTTGTGCCGG
            841 CATCTAATGT TTGCGCCTGC GTCTGTACTA GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC
            901 CCGTGGTGGA ACTGACGAGT TCGGAACACC CGGGCCGCAA CCCTGGGAGA CGTCCCAGGG
            961 ACTTCGGGGG CCGTTTTTGT GGCCCGACCT GAGTCCAAAA TCCCGATCGT TTTGACTCTT
           1021 TGGTGCACCC CCTATAGAGG ATTATTGTGA TCTGGTAGAG ACGAGACTAA AAAGTCGCTT
          1081 CGTCTGATTT TTGCTTCGAT TTGGACCGAA GTC
          //
          Primer: pMFG_1
          Sequence file: RDB12181_A6Aje.seq check
          >12181_12181_A6Aj_pMFG-1_G09_21.ab1
              1 GCATTGGGGG GAAAGGGCTG TTAGTAACTC TTGTCATGTC AGGGTCAGGG AAGTTTACAA
             61 GGGGCGGGGG TGCCCCATAT AAGATCTCAT ATGGCCATAT GGGGGCGCCT AGAGAAGGAG
            121 TGAGGGCTGG ATAAAGGGAG GATCGAGGCG GGGTCGAACG AGGAGGTTCA AGGGGGAGAG
            181 ACGGGGCGGA TGGAGGAAGA GGAGGCGGAG GCTTAGGGTG TACAAAGGGC TTGACCCAGG
            241 GAGGGGGGTC AAAAGCCAAG GCTTCCCAGG TCACGATGTA GGGGACCTGG TCTGGGTGTC
            301 CATGCGGGCC AGGTGAAAAG ACCTTGATCT TAACCTGGGT GATGAGGTCT CGGTTAAAGG
            361 TGCCGTCTCG CGGCCATCCG ACGTTAAAGG TTGGCCATTC TGCAGAGCAG AAGGTAACCC
            421 AACGTCTCTT CTTGACATCT ACCGACTGGT TGTGAGCGAT CCGCTCGACA TCTTTCCAGT
            481 GACCTAAGGT CAAACTTAAA GGAGTGGTAA CAGTCTGGCC CTAATTTTCA GACAAATACA
            541 GAAACACAGT CAGACAGAGA CAACACAGAA CGATGCTGCA GCAGACAAGA CGCGCGGCGC
            601 GGCTTCGGTC CCAAACCGAA AGCAAAATTC AGACGGAGCG GGAACTGTTT AGTTCTCGTC
            661 TCCTACCAGA ACCACATATC CCTCCTATTA GGGGGGTGCA CCAAAGAAGT CAAACGATCG
            721 GGATTTTGGA CTCAGTCGGG CCACAAAAAC GGCCCCGGAG TCCTGGGACG TCTCCAAGTG
            781 CGCGGTGTTC GACTCGTCAG TTCCACCACG GTCCGCCAAA TACAGAGCTA GTTAGCTTAA
            841 CTTAGTTACA GACGCAAGGC TAAACATAAA ATGGCGGCCA AACCGCTCAG AACGAACGAA
            901 CCGAACGACG CTGGCACTAA CCTCCGCGGG GATCCGGTCC TTGCAGGGTT CTAATCCTGG
          961 ACTCCATAGA AAGACCTGAG TGGGCTCTAC TTCAAGACCT CAG
          //
          Primer: EGFP-C
          Sequence file: RDB12181_A6Ajf.seq check
          >12181_12181_A6Aj_EGFP_C_G02_20.ab1
              1 CGAAGGGTTA CTTTCGGGCA TGGACGAGCT GTACAAGTAA AGCGGCCGGC CGCCAGCACA
             61 GTGGTCGACC GGGCCGCCAG CACAGTGGTC GATCGAGGGG GGGCCCGCGA TTAGTCCAAT
            121 TTGTTAAAGA CAGGATATCA GTGGTCCAGG CTCTAGTTTT GACTCAACAA TATCACCAGC
            181 TGAAGCCTAT AGAGTACGAG CCATAGATAG AATAAAAGAT TTTATTTAGT CTCCAGAAAA
            241 AGGGGGGAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT
            301 GCAAGGCATG GAAAATACAT AACTGAGAAT AGAGAAGTTC AGATCAAGGT TAGGAACAGA
            361 GAGACAGCAG AATATGGGCC AAACAGGATA TCTGTGGTAA GCAGTTCCTG CCCCGGCTCA
            421 GGGCCAAGAA CAGTTGGAAC AGCAGAATAT GGGCCAAACA GGATATCTGT GGTAAGCAGT
            481 TCCTGCCCCG GCTCAGGGCC AAGAACAGAT GGTCCCCAGA TGCGGTCCCG CCCTCAGCAG
            541 TTTCTAGAGA ACCATCAGAT GTTTCCAGGG TGCCCCAAGG ACCTGAAATG ACCCTGTGCC
            601 TTATTTGAAC TAACCAATCA GTTCGCTTCT CGCTTCTGTT CGCGCGCTTC TGCTCCCCGA
            661 GCTCAATAAA AAGAGCCCAC AACCCCTCAC TCGGCGCGCC AGTCCTCCGA TAGACTGCGT
            721 CGCCCGGGTA CCCGTGTATC CAATAAACCC TCTTGCAGTT GCATCCGACT TGTGGTCTCG
            781 CTGTTCCTTG GGAGGGTCTC CTCTGAGTGA TTGACTACCC GTCAGCGGGG GTCTTTCACA
            841 TGCAGCATGT ATCAAATTAA TTTGGTTTTT TTTTCTTAAA GTATTTACAT TAAATGGCCC
            901 ATAGTTTGCA TTAATGAAAT CGGGCCAACG GCGCGGGGAG AGGCCGGTTT GCGTATTGGG
            961 CGCTCTTTCC GCTTCCTCGC TCCACTGACT CGCTGCGCCT CGGTCGTTCG GCCTGGCGGG
           1021 CGAGCCGTAT CAGCTTCAAC TCAAAAGGCG TAATACGGTT ATTCCACAGG AATTCAGGAA
          1081 TACGCCAGGC AGGACCATGT GAGCCAAGGC CAGCCAAAGC CAGGAACGCG TATA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ota, M., Mammalian Tead proteins regulate cell proliferation and contact inhibition as transcriptional mediators of Hippo signaling. Development 135, 4059-4069 (2008). PMID 19004856.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl