Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMYs-HA-Yap1 DTead BD-IRES-EGFP

Retroviral vector of mouse Yap1, deletion of Tead BD

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008)) and an acknowledgment to the DEPOSITOR are requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization. Additional terms and conditions: Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
Ordering	Please visit Information of Request for Distribution.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Remember that you will be working with samples containing infections virus.

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献 (Ota, M., Sasaki, H., Development, 135, 4059-4069 (2008))を引用し、謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。 付加的提供条件： pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12178_A6Ajp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: 5'Retro (pFB-5') Sequence file: RDB12178_A6Aja.seq
>12178_12178_A6Aj_5'Retro(pFB-5')_A10_01.ab1     1 CGCCGCTAGG GGGTTTTTTC TTCGGACTGC TAGTTATTAA GGATCCATGT ACCCATACGA    61 CGTTCCAGAT TACGCTGGTA CCGACCCCGC GCAACAGCCG CCGCCCCAGC CGGCCCCGCA   121 AGGCCCCGCG CCGCCGTCCG TGTCTCCGGC CGGGACCCCC GCGGCCCCGC CCCTGACTCC   181 ACAGCATGTT CGAGCTCACT CCTCTCCAGC CTCCCTGCAG CTGGGTGCCG TTTCTCCTGG   241 GACACTCACA GCCAGTGGCG TTGTCTCTGG CCCTGCCGCT GCCCCTGCAG CTCAGCATCT   301 CCGGCAGTCC TCCTTTGAGA TCCCTGATGA TGTACCACTG CCAGCAGGCT GGGAGATGGC   361 CAAGACATCT TCTGGTCAAA GATACTTCTT AAATCACAAC GATCAGACAA CAACATGGCA   421 GGACCCCCGG AAGGCCATGC TTTCGCAACT GAACGTTCCT GCGCCTACCA GCCCAGCGGT   481 GCCCCAGACG CTGATGAATT CTGCCTCAGG ACCTCTTCCT GATGGATGGG AGCAAGCCAT   541 GACTCAGGAT GGAGAAGTTT ACTACATAAA CCATAAGAAC AAGACCACAT CCTGGCTGGA   601 CCCAAGGCTG GACCCTCGTT TTGCCATGAA CCAGAGGATC ACTCAGAGTG CTCCAGTGAA   661 GCAGCCCCCA CCCTTGGCTC CCCAGAGCCC ACAGGGAGGC GTCCTGGGTG GAGGCAGTTC   721 CAACCAGCAG CAGCAAATAC AGCTGCAGCA GTTACAGATG GAGAAGGAGA GACTGCGGTT   781 GAAACAACAG GAATTATTTC GGCAGGAATT AGCTCTGCGC AGCCAGTTGC CTACACTGGA   841 GCAGGATGGA GGGACTCCGA ATGCAGTGTC TTCTCCTGGG ATGTCTCAGG AATTGAGAAC   901 AATGACAACC AATAGTTCCG ATCCCTTTCT TAACAGTGGC ACCTATCACT CTCGAGATGA   961 GAGCACAGAC AGCGGCCTCA GCATGAGCAG CTACAGCATC CCTTCGGGAC CCCAGACGAC  1021 TTTCTCACAG TGTGATGAAA TGGATACAGG AGAACATCAT CAGCAAGCAC CCTTGCCGTC  1081 ACAGCAGAGT CGCTTCCCGA CTACCTGGAA GCTCTGGAAC AATGTGAACC TTGGCCACCA 1141 CCTGGAAAAG GAC //
Primer: IRES-R Sequence file: RDB12178_A6Ajb.seq
>12178_12178_A6Aj_IRES-R_C08_08.ab1     1 GCATGGCGGG GCTCGGCCAG TACGTTAGGG GGGGGGGAGG GAGAGGGGGG GGGGGGGCGG    61 AATTTACGTA GCGGCCGCCT ATAACCACGT GAGAAAGCTT TCTTTATCTA GCTTGGTGGC   121 AGCCAACACA GACTCCACGT CCAAGATTTC GGAACTCAGC GCTTCCTGCA GACTGGGCAT   181 CAGCTCCTCC CCTTCTATGT TCATTGCATC TCCTTCCAGT GTGCCAAGGT CCACATTTGT   241 CCCAGGGAGG GCTTCCAGGT AGTCGGGGAA GCGGCTCTGC TGTGACGGCA GGGTGCTTTG   301 GCTGATGGTG TCTCCTGTAT CCATTTCATC CACACTGTTG AGGAAGTCGT CTGGGGTCCG   361 AGGGATGCTG TAGCTGCTCA TGCTGAGGCC GCTGTCTGTG CTCTCATCTC GAGAGTGATA   421 GGTGCCACTG TTAAGAAAGG GATCGGAACT ATTGGTTGTC ATTGTTCTCA ATTCCTGAGA   481 CATCCCAGGA GAAGACACTG CATTCGGAGT CCCTCCATCC TGCTCCAGTG TAGGCAACTG   541 GCTGCGCAGA GCTAATTCCT GCCGAAATAA TTCCTGTTGT TTCAACCGCA GTCTCTCCTT   601 CTCCATCTGT AACTGCTGCA GCTGTATTTG CTGCTGCTGG TTGGAACTGC CTCCACCCAG   661 GACGCCTCCC TGTGGGCTCT GGGGAGCCAA GGGTGGGGGC TGCTTCACTG GAGCACTCTG   721 AGTGATCCTC TGGTTCATGG CAAACGAGGG TCCAGCCTTG GGTCCAGCCA GGATGTGGTC   781 TTGTTCTATG GTTATGTAGT AACTTCTCCA TCCTGGAGTC ATGCTGCTCC CATCATCAGA   841 AGAGGTCTGA GCAGAATCAT CACGTTCTGG GGCACCCGCT GGCCTGTAGG CCAGAACGTC   901 AGTGCGAGCC ATGGCCTCGG GTCCTGCATG TGTGTCTGGA TCGTTGGGAT CAGAGGATCT   961 CGACGAAGTG TCTGGCATTC AGCTGTGCAT GTACTTCAGG ATCCAGAACT GCGAATGCTC 1021 GA //
Primer: IRES-F Sequence file: RDB12178_A6Ajc.seq
>12178_12178_A6Aj_IRES-F_B10_04.ab1     1 GGCAATGCCT TTTAATGTGT TTAGTCGAGG TTAAAAAACG TCTAGGCCCC CCGAACCACG    61 GGGACGTGGT TTTCCTTTGA AAAACACGAT GATAATATGG CCACAACCAT GGTGAGCAAG   121 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC   181 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC   241 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC   301 CTGACCTACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC   361 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC   421 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC   481 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC   541 AACTACAACA GCCACAACGT CTATATCATG GCCGACAAGC AGAAGAACGG CATCAAGGTG   601 AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA CCACTACCAG   661 CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA CCTGAGCACC   721 CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT GCTGGAGTTC   781 GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTAAAG CGGCCGGCCG   841 CCAGCACAGT GGTCGACCGG GCCGCCAGCA CAGTGGTCGA TCGAGGGGGG GCCCGCGATT   901 AGTCCAATTT GTTAAAGACA GGATATCAGT GGTCCAGGCT CTAGTTTTGA CTCAACAATA   961 TCACCAGCTG AAGCCTATAG AGTACGAGCC ATAGAATAGA ATAAAAAGAT TTTAATTAGT  1021 CCTCCAGAAA AAGGGGGGGA ATGAAAGACC CCCAACCTTG TAGTTTGGGC AAAGCTAGCT  1081 TAGGTAACGG CCAATTTTGC AAAGGCCGAT GGAAAATACA ATTAACCTGA AGAATGAGAG 1141 AAGTTCAGAA ATTCAAGGTT TTAGAGAC //
Primer: Amp_R Sequence file: RDB12178_A6Ajd.seq
>12178_12178_A6Aj_Amp_R_D08_11.ab1     1 ATCTTTTTAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT    61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA   121 CCTGCATAAT GAAAGACCCC ACCTGTAGGT TTGGCAAGCT AGCTTAAGTA ACGCCATTTT   181 GCAAGGCATG GAAAAATACA TAACTGAGAA TAGAAAAGTT CAGATCAAGG TCAGGAACAG   241 ATGGAACAGC TGAATATGGG CCAAACAGGA TATCTGTGGT AAGCAGTTCC TGCCCCGGCT   301 CAGGGCCAAG AACAGATGGA ACAGCTGAAT ATGGGCCAAA CAGGATATCT GTGGTAAGCA   361 GTTCCTGCCC CGGCTCAGGG CCAAGAACAG ATGGTCCCCA GATGCGGTCC AGCCCTCAGC   421 AGTTTCTAGA GAACCATCAG ATGTTTCCAG GGTGCCCCAA GGACCTGAAA TGACCCTGTG   481 CCTTATTTGA ACTAACCAAT CAGTTCGCTT CTCGCTTCTG TTCGCGCGCT TCTGCTCCCC   541 GAGCTCAATA AAAGAGCCCA CAACCCCTCA CTCGGCGCGC CAGTCCTCCG ATTGACTGAG   601 TCGCCCGGGT ACCCGTATTC CCAATAAAGC CTCTTGCTGT TTGCATCCGA ATCGTGGACT   661 CGCTGATCCT TGGGAGGGTC TCCTCAGATT GATTGACTGC CCACCTCGGG GGTCTTTCAT   721 TTGGAGGTTC CACCGAGATT TGGAGACCCC TGCCCAGGGA CCACCGACCC CCCCGCCGGG   781 AGGTAAGCTG GCCAGCGGTC GTTTCGTGTC TGTCTCTGTC TTTGTGCGTG TTTGTGCCGG   841 CATCTAATGT TTGCGCCTGC GTCTGTACTA GTTAGCTAAC TAGCTCTGTA TCTGGCGGAC   901 CCGTGGTGGA ACTGACGAGT TCGGAACACC CGGCCGCAAC CCTGGGAGAC GTCCCAAGGG   961 ACTTCGGGGG CCGTTTTTTG TGGCCCGGAC CTGAAGTCCC AAAATTCCCG ATCGTTTTGG  1021 ACTTCTTTGG TGCACCCCTA TAGGAGGGAT ATGGTGGTTC TGGGTAGAGA CGAAGAACTT 1081 AAACAGTCCC GGCCTCCGTC CTGAATTATT GCTTTTCGAG TTTGGGGAAC CGGAAAAGG //
Primer: pMFG_1 Sequence file: RDB12178_A6Aje.seq
>12178_12178_A6Aj_pMFG-1_E08_14.ab1     1 GCGGGGAGAG GGCTGTTAGT ACTCTTGTCA TGTCAGGGTC AGGGAAGTTT ACAAGGGGCG    61 GGGGTGCCCC ATATAAGATC TCATATGGCC ATATGGGGGC GCCTAGAGAA GGAGTGAGGG   121 CTGGATAAAG GGAGGATCGA GGCGGGGTCG AACGAGGAGG TTCAAGGGGG AGAGACGGGG   181 CGGATGGAGG AAGAGGAGGC GGAGGCTTAG GGTGTACAAA GGGCTTGACC CAGGGAGGGG   241 GGTCAAAAGC CAAGGCTTCC CAGGTCACGA TGTAGGGGAC CTGGTCTGGG TGTCCATGCG   301 GGCCAGGTGA AAAGACCTTG ATCTTAACCT GGGTGATGAG GTCTCGGTTA AAGGTGCCGT   361 CTCGCGGCCA TCCGACGTTA AAGGTTGGCC ATTCTGCAGA GCAGAAGGTA ACCCAACGTC   421 TCTTCTTGAC ATCTACCGAC TGGTTGTGAG CGATCCGCTC GACATCTTTC CAGTGACCTA   481 AGGTCAAACT TAAGGGAGTG GTAACAGTCT GGCCCTAATT TTCAGACAAA TACAGAAACA   541 CAGTCAGACA GAGACAACAC AGAACGATGC TGCAGCAGAC AAGACGCGCG GCGCGGCTTC   601 GGTCCCAAAC CGAAAGCAAA AATTCAGACG GAGGCGGGAA CTGTTTTAGG TTCTCGTCTC   661 CTACCAGAAC CACATATCCC TCCTATTAGG GGGGTGCACC AAAGAGTCCA AAACGATCGG   721 GATTTTTGGA CTCAGGTCGG GCCACAAAAA CGGCCCCCGA AGTCCCTGGG ACGTCTCCCA   781 GGGTTGCGGC CGGGTGTTCC GAACTCGTCA GTTCCACCAC GGGTCCGCCA GATACAGAGC   841 TAGTTAGCTA ACTAGTACAG ACGCAGGCGC AAACATTAGA TGCCGGCACA AACACGCACA   901 AAGACAGAGA CAGACACGAA ACGACCGCTG GGCCAGCTTA CCTCCCGGCG GGGGGGTCGG   961 TGGTCCCTGG GCAGGGGTCT CCAATCTCGG TGGACTCGAA ATGAAGACCC CCGAGTGGGC  1021 AGTCCATTCA TCTGAAGAGA CCTCCAAGAT CAGCGAGTTC ACGATCAGAT GCACAGCAGA 1081 GCCTTTATGG AATACGGTTA CCCGGGCGCA //
Primer: EGFP-C Sequence file: RDB12178_A6Ajf.seq
>12178_12178_A6Aj_EGFP-C_F08_17.ab1     1 CAGGGCACTC TCGGCATGGA CGAGCTGTAC AAGTAAAGCG GCCGGCCGCC AGCACAGTGG    61 TCGACCGGGC CGCCAGCACA GTGGTCGATC GAGGGGGGGC CCGCGATTAG TCCAATTTGT   121 TAAAGACAGG ATATCAGTGG TCCAGGCTCT AGTTTTGACT CAACAATATC ACCAGCTGAA   181 GCCTATAGAG TACGAGCCAT AGATAGAATA AAAGATTTTA TTTAGTCTCC AGAAAAAGGG   241 GGGAATGAAA GACCCCACCT GTAGGTTTGG CAAGCTAGCT TAAGTAACGC CATTTTGCAA   301 GGCATGGAAA ATACATAACT GAGAATAGAG AAGTTCAGAT CAAGGTTAGG AACAGAGAGA   361 CAGCAGAATA TGGGCCAAAC AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCAGGGC   421 CAAGAACAGT TGGAACAGCA GAATATGGGC CAAACAGGAT ATCTGTGGTA AGCAGTTCCT   481 GCCCCGGCTC AGGGCCAAGA ACAGATGGTC CCCAGATGCG GTCCCGCCCT CAGCAGTTTC   541 TAGAGAACCA TCAGATGTTT CCAGGGTGCC CCAAGGACCT GAAATGACCC TGTGCCTTAT   601 TTGAACTAAC CAATCAGTTC GCTTCTCGCT TCTGTTCGCG CGCTTCTGCT CCCCGAGCTC   661 AATAAAAGAG CCCACAACCC CTCACTCGGC GCGCCAGTCC TCCGATAGAC TGCGTCGCCC   721 GGGTACCCGT GTATCCAATA AACCCTCTTG CAGTTGCATC CGACTTGTGG TCTCGCTGTT   781 CCTTGGGAGG GTCTCCTCTG AGTGATTGAC TACCCGTCAG CGGGGGTCTT TCACATGCAG   841 CATGTATCAA AATTAATTTG GTTTTTTTTC TTAAGTATTT ACATTAAATG GCCATAGTTG   901 CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT   961 TCCTCGCTCA CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGG TATCAGCTCA  1021 CTCAAAGGCG GGTAATACGG GTTATCCCAC AGAATTCAGG GGATTACGCA GAAAAGAACA 1081 TGTGGAGCCA AACGCCCAGG CCAAAGGGCC AGGAACCGTA AAAAAAAGGC TGC //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.04.10

GNP_filter3_RDBDEP_html_230405.pl