Please review the QC test results indicated by icon below as well as clone information before placing your order.

pPyCAG-BstXI-IB

Mammalian expression vector. Blasticidin S resistance.

Sequence	RDB12107z.seq
Remarks, protocol and/or map (gif)	RDB12107a.gif

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution

In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.

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Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。 付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

Reference section:

Further references such as user reports and related articles (go to bottom)

References and tips

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB12107_A5L9p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 () Sequence file: RDB12107_A5L9a.seq
>RDB12107_12107_A5L9_pAxCA_F1_A10_01.ab1     1 CGGGGAAAAA GACGGGGTAC TTCTCATGTT ATGCCTTCTT CTTTTTCCTA CAGCTCCTGG    61 GCAACGTGCT GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCCTC GAGATCCATT   121 GTGCTGGCGG TAGGCGAGCA GCGCCTGCCT GAAGCTGCGG GCATTCCCGA TCAGAAATGA   181 GCGCCAGTCG TCGTCGGCTC TCGGCACCGA ATGCGTATGA TTCTCCGCCA GCATGGCTTC   241 GGCCAGTGCG TCGAGCAGCG CCCGCTTGTT CCTGAAGTGC CAGTAAAGCG CCGGCTGCTG   301 AACCCCCAAC CGTTCCGCCA GTTTGCGTGT CGTCAGACCG TCTACGCCGA CCTCGTTCAA   361 CAGGTCCAGG GCGGCACGGA TCACTGTATT CGGCTGCAAC TTTGTCATGC TTGACACTTT   421 ATCACTGATA AACATAATAT GTCCACCAAC TTATCAGTGA TAAAGAATCC GCGCCAGCAC   481 AATGGATCTC GAGGAATTCA CTCCTCAGGT GCAGGCTGCC TATCAGAAGG TGGTGGCTGG   541 TGTGGCCAAC TGGCTCACAA ATACCACTGA GATCGACGGT ATCGAGCGGC CGCTCGATAA   601 GCTTGATATC GAATTCCGCC CCCCCCCCCT CTCCCTCCCC CCCCCCTAAA CGTTACTGGC   661 CGAAGCCGCT TGGAATAAGG CCGGTGTGCG TTTGTCTATA TGTTATTTTC CACCATATTG   721 CCGTCTTTTG GCAATGTGAG GGCCCGGAAA CCCTGGCCCT GTCTTCTTGA CGAGCATTCC   781 TAGGGGTCTT TCCCCTCTCG CCAAAGGAAT GCAAGGTCTG TTTGAATGTC GTGAAGGAAG   841 CAGTTCCTCT GGGAAGCTTC TTGAAGACAA ACAACGTCTG TAGCGACCCT TTGCAGGCAG   901 CGGAACCCCC CACCTGGCGA CAGGTGCCTC TGCGGGCCAA AGCCACGTGT ATAAGATACA   961 CTTGCAAAGG CGGCACAACC CCCAGTGCCA CGTTGTGAGT TGGGATAGTT GTGGAAAAGA  1021 GTTCAAATGG CCTCTCCTCC AGCGTATTTC AACAGGGGGC TTGAAAGATG CCCAGAAAGG  1081 TACCCCATTG TATGGGATCT GATTCTGGGG GTCCTCAGTG CACATGCCTT TACATGGTGT  1141 TTTAGTCCGA GATAAAACCG TGTTCTAGGT CCTTCCTGAT CCCCGTGGGA CCGGTTGCTT 1201 TTTCTCTTTT TGTGAAAAAC GAC //
Primer: IRES_F () Sequence file: RDB12107_A5L9b.seq
>RDB12107_12107_A5L9_IRES_F_B10_04.ab1     1 TGAAATCATT AATGTGTTTA GTCGAGGTTA AAAAACGTCT AGGCCCCCCG AACCACGGGG    61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGCC TTTGTCTCAA   121 GAAGAATCCA CCCTCATTGA AAGAGCAACG GCTACAATCA ACAGCATCCC CATCTCTGAA   181 GACTACAGCG TCGCCAGCGC AGCTCTCTCT AGCGACGGCC GCATCTTCAC TGGTGTCAAT   241 GTATATCATT TTACTGGGGG ACCTTGTGCA GAACTCGTGG TGCTGGGCAC TGCTGCTGCT   301 GCGGCAGCTG GCAACCTGAC TTGTATCGTC GCGATCGGAA ATGAGAACAG GGGCATCTTG   361 AGCCCCTGCG GACGGTGCCG ACAGGTGCTT CTCGATCTGC ATCCTGGGAT CAAAGCCATA   421 GTGAAGGACA GTGATGGACA GCCGACGGCA GTTGGGATTC GTGAATTGCT GCCCTCTGGT   481 TATGTGTGGG AGGGTTAACT AGATAAGTAA TGATCATAAT CAGCCATATC ACATCTGTAG   541 AGGTTTTACT TGCTTTAAAA AACCTCCCAC ACCTCCCCCT GAACCTGAAA CATAAAATGA   601 ATGCAATTGT TGTTGTTAAC TTGTTTATTG CAGCTTATAA TGGTTACAAA TAAAGCAATA   661 GCATCACAAA TTTCACAAAT AAAGCATTTT TTTCACTGCA TTCTAGTTGT GGTTTGTCCA   721 AACTCATCAA TGTATCTTAT CATGTCTGGA TCCGCTGCAT TAATGAATCG GCCAACGCGC   781 GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG   841 CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC   901 CACAGAATCA AGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG   961 GAACCGTAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT  1021 CACAAAAATC GACGCTCAAG TCAGAGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG  1081 CGTTTCCCCC TGAAGCTCCC TCGTGCGCCT CCTCCTGGTC GAACCCTGCG GCTACGGATA 1141 CCTGTTCCGC CTTCTCTCCC CTTCGGAAGC CGTGTGCCGC GCCCT //
Primer: Amp_R () Sequence file: RDB12107_A5L9c.seq
>12107_12107_A9EkF_Amp_R_A10_01_ABI08.ab1     1 CTAAGGTGGC CCGGGCTTTG GGAAAACAAA GACTGTATTT CCTGGAAATT AATGTTTATT    61 CAATAAACTG TGTATTCAGC TATATTCACA TAGTGGTGAG GCTGAAATGA GGCGGGAAGA   121 GGCGGTTGGG GCTTAATTAT ATCAATTTGG GTGGCCCCAC AGCGCCTCCA AGGCGCCAGT   181 CCTGTTTTGA CAAGTTGCCT CTGGAAGCCT CTCTTCTTTT TCTCCAGAGT AAGCGGAGGC   241 CAGGGGCCCC CGGCCTCTGC TTAATACTAA AAAAAACAGC TGTTGTCATA GTAATGATTG   301 GGTGGAAACA TTCCAGGCCT GGGTGGAGAG GCTTTTTGCT TCCTCTTGCA AAACCACACT   361 GACATTCCAG GCCTGGGTGG AGAGGCTTTT TGCTTCCTCT TGCAAAACCA CACTGCCCTC   421 TGGAGGGCAG TTGCCTAGCA ACTAATTAAA AGAGGATGTC GCACGGCCAG CTGCGGTCAG   481 TTAGTCACTT CCTGCTTAAC TGACTTGACA TTTTCTATTT TAAGAGTCGG GAGGAAAATT   541 ACTGTGTTGG AGGCCCTCCG CCATCTTCTG AAGCTGAATC GAATTAACTT GTTTATTGCA   601 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT   661 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGGATC   721 TGATATCATC GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA   781 TTAGTTCATA GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT   841 GGCTGACCGC CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA   901 ACGCCAATAG GGACTTTCCA TTGACGTCAA TGGGTGGAGT ATTTACGGTA AACTGCCCAC   961 TTGGCAGTAC ATCAAGTGTA TCATATGCCC AAGTACGCCC CCCTATTGAC GTCAATGACG  1021 GTAAATGGGC CCGCCTGGCA TTATGCCCAG TACATGACCC TTATGGACTT TCTTACTGGG  1081 CAGTACATCT ACGTATAGGT CAATCGCCTA TTACCCAATT GGGTCGAAGG GGTGAGCTCC 1141 CAACCGCGT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.02

GNP_filter3_RDBDEP_html_230501.pl