Please review the QC test results indicated by icon below as well as clone information before placing your order.

pPyCAG-cGR-IP deltaN deltaS SVOri

Expression vector of glucocorticoid receptor ligand binding domain

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (BMC Dev Biol. 7:80(2007)).
Additional terms and conditions for distribution	Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) is requested.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供案内 (日本国内) [open/close]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (BMC Dev Biol. 7:80(2007))。
付加的提供条件	CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用を必要とする。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Sequence	RDB10440z.seq
Remarks, protocol and/or map (gif)	RDB10440a.gif

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB10440_A6Gqp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB10440_A6Gqa.seq
>10440_10440_A6Gq_6_pAxCA_F1_A12_03.ab1     1 CTCACATTGT TGGGTCTGCT CATGTTATGC CTTCTTCTTT TTCCTACAGC TCCTGGGCAA    61 CGTGCTGGTT ATTGTGCTGT CTCATCATTT TGGCAAAGAA TTCCTCGAGA TCTCACGCGT   121 GACTAGTGCC ATGGAAAATC CTGGTAACAA AACAATAGTT CCTGCAACGT TACCACAACT   181 CACCCCTACC CTGGTGTCAC TGTTGGAGGT TATTGAACCT GAAGTGTTAT ATGCAGGATA   241 TGATAGCTCT GTTCCAGACT CAACTTGGAG GATCATGACT ACGCTCAACA TGTTAGGAGG   301 GCGGCAAGTG ATTGCAGCAG TGAAATGGGC AAAGGCAATA CCAGGTTTCA GGAACTTACA   361 CCTGGATGAC CAAATGACCC TACTGCAGTA CTCCTGGATG TTTCTTATGG CATTTGCTCT   421 GGGGTGGAGA TCATATAGAC AATCAAGTGC AAACCTGCTG TGTTTTGCTC CTGATCTGAT   481 TATTAATGAG CAGAGAATGA CTCTACCCTG CATGTACGAC CAATGTAAAC ACATGCTGTA   541 TGTTTCCTCT GAGTTACACA GGCTTCAGGT ATCTTATGAA GAGTATCTCT GTATGAAAAC   601 CTTACTGCTT CTCTCTTCAG TTCCTAAGGA CGGTCTGAAG AGCCAAGAGC TATTTGATGA   661 AATTAGAATG ACCTACATCA AAGAGCTAGG AAAAGCCATT GTCAAGAGGG AAGGAAACTC   721 CAGCCAGAAC TGGCAGCGGT TTTATCAACT GACAAAACTC TTGGATTCTA TGCATGAAGT   781 GGTTGAAAAT CTCCTTAACT ATTGCTTCCA AACATTTTTG GATAAGACCA TGTCCATCGA   841 GTTCCCCGAG ATGTTAGCTG AAATCATCAC CAATCAGATA CCAAAATATT CAAATGGAAA   901 TATCAAAAAA CTTCTGTTTC ATCAAAAGTG AGCGGCCGCT CGATAAGCTT GATATCGAAT 961 TCCGCCGCCT CCCCTTTTTG TCGAGTC //
Primer: IRES-F Sequence file: RDB10440_A6Gqb.seq
>10440_10440_A6Gq_6_IRES-F_C12_09.ab1     1 GGAGGCGTTC TGTGTTTAGT CGAGGTTAAA AAACGTCTAG GCCCCCCGAA CCACGGGGAC    61 GTGGTTTTCC TTTGAAAAAC ACGATGATAA TATGGCCACA ACCATGACCG AGTACAAGCC   121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC   181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT   241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT   301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC   361 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA   421 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC   481 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC   541 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG   601 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA   661 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGACGCCCGC CCCACGACCC   721 GCAGCGCCCG ACCGAAAGGA GCGCACGACC CCATGCATCG ATGATCTAGA GCTCGCTGAT   781 CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT   841 CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT   901 CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGGTG GGGTGGGGCA GGACAGCAAG   961 GGGGAGGATT GGGAAGACAA TAGCAGGCAT GCTGGGGATG CGGTGGCTCT ATGGGCTTCT  1021 GAGCGGAAAG AACCTGCAGC CCCAAGCTGC GTATCATGGG TCATAGCTGT TTCTGTGTGA  1081 AATTGTATCC GCTCACATCC ACACAACCAT ACGAGTCGGC AAGGCAATAA AGGTGTTAGC //
Primer: Amp_R Sequence file: RDB10440_A6Gqc.seq
>10440_10440_A6Gq_6_Amp_R_D12_12.ab1     1 TTCGTTTCAT GACCGCTTTG GAAAACAAAG ACTGTATTTC CTGGAAATTA ATGTTTATTC    61 AATAAACTGT GTATTCAGCT ATATTCACAT AGTGGTGAGG CTGAAATGAG GCGGGAAGAG   121 GCGGTTGGGG CTTAATTATA TCAATTTGGG TGGCCCCACA GCGCCTCCAA GGCGCCAGTC   181 CTGTTTTGAC AAGTTGCCTC TGGAAGCCTC TCTTCTTTTT CTCCAGAGTA AGCGGAGGCC   241 AGGGGCCCCC GGCCTCTGCT TAATACTAAA AAAAACAGCT GTTGTCATAG TAATGATTGG   301 GTGGAAACAT TCCAGGCCTG GGTGGAGAGG CTTTTTGCTT CCTCTTGCAA AACCACACTG   361 ACATTCCAGG CCTGGGTGGA GAGGCTTTTT GCTTCCTCTT GCAAAACCAC ACTGCCCTCT   421 GGAGGGCAGT TGCCTAGCAA CTAATTAAAA GAGGATGTCG CACGGCCAGC TGCGGTCAGT   481 TAGTCACTTC CTGCTTAACT GACTTGACAT TTTCTATTTT AAGAGTCGGG AGGAAAATTA   541 CTGTGTTGGA GGCCCTCCGC CATCTTCTGA AGCTGAATCG AATTAACTTG TTTATTGCAG   601 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT   661 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGATCT   721 GATATCATCG TCGACATTGA TTATTGACTA GCTAGTTATT AATAGTAATC AATTACGGGG   781 TCATTAGTTC ATAGCCCATA TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG   841 CCTGGCTGAC CGCCCAACGA CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA   901 GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC   961 CACTTGGCAG TACATCAAGT GTATCATATG CCCAAGTACG CCCCCCTATT GACGTCATGA  1021 CGGTAAATGG GCCCGCCTGG CATTATGCCC AGTACATGAC CTTATGGGGA CTTTTCTACT  1081 GGCAGTACAT CTACGTATAG TCATCGGCTA TTACAATGAA TGGATCGAGT GTGAAGCCCC 1141 CCG //
Primer: BGHpA_F Sequence file: RDB10440_A6Gqd.seq
>10440_10440_A6Gq_6_BGHpA-F_E12_15.ab1     1 TTGGAATGGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG    61 GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG   121 AACCTGCAGC CCAAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA   181 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC   241 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG   301 AAACCTGTCG TGCCAGCGGA TCCGCATCTC AATTAGTCAG CAACCATAGT CCCGCCCCTA   361 ACTCCGCCCA TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGCATGG   421 CTGACTAATT TTTTTTATTT ATGCAGAGGC CGAGGCCGCC TCGGCCTCTG AGCTATTCCA   481 GAAGTAGTGA GGAGGCTTTT TTGGAGGCCT AGGCTTTTGC AAAAAGCTAA CTTGTTTATT   541 GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT   601 TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGG   661 ATCCGCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG CGGTTTGCGT ATTGGGCGCT   721 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT   781 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCACGAAAGA   841 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT   901 TTTTCCATAG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG   961 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCCTGGAAGC TCCCTCGTGC  1021 GCTCTCCTGT TTCCGACCCT GCCGCCTTAC CGGATACCTG TCCGCCTTTT CTCCCTTTCA  1081 GGAGCGTGAC GCTTTTCTCA TAGCTTCACG CTGGTAAGTA TCCTCAGGTT CGGGTTGTTA 1141 AGTCCGTTCC GCGCCTCTCC AG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl