RDB10004 - pLefty1p-luc

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pLefty1p-luc

Oct-Sox enhancer carrying reporter derived from Lefty1 promoter.

Catalog number	RDB10004
Resource name	pLefty1p-luc
Alternative name	stock no 972
Clone info.	Reporter construct with luciferase expression under the Lefty1 promoter.
Comment	Genomic fragment was amplified by PCR with primers 5'- CCTTTACACTGGTCTCGAGCC-3' and 5'-ATAAGCTTGTCCGGGAAGAGGAGCCTTG-3'. Resulting fragment contains 1,379 bp from -1297 to +82 relative to the transcription start site of the Lefty1. See Fig.1 of Nakatake, Y., Mol. Cell. Biol., 26 (20), 7772-7782, 2006.
Vector backbone	pGL3-basic (Plasmid)
Gene/insert name	Mouse Lefty1 genomic DNA
Depositor\|Developer	Niwa, Hitoshi \|

Other clones in our bank

mouse Lefty1

External Database
Mouse Lefty1

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nakatake, Y. et al., Mol. Cell. Biol., 26, 7772-7782 (2006)).

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Nakatake, Y. et al., Mol. Cell. Biol., 26, 7772-7782 (2006)).
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakatake, Y. et al., Mol. Cell. Biol., 26, 7772-7782 (2006))。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB10004	pLefty1p-luc	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original	Nakatake, Y., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384.

Further references such as user reports and related articles (go to bottom)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB10004_A9C7p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGL3basic Sequence file: RDB10004_A9C7a.seq
>10004_10004_A9C7_3_pGL3basic_4406F_A01_01_ABI08.ab1 1 GTTTTTGGCG GATTTCGGCC TATTGGTTAA AAATGAGCTG ATTTAACAAA AATTTAACGC 61 GAATTTTAAC AAAATATTAA CGCTTACAAT TTGCCATTCG CCATTCAGGC TGCGCAACTG 121 TTGGGAAGGG CGATCGGTGC GGGCCTCTTC GCTATTACGC CAGCCCAAGC TACCATGATA 181 AGTAAGTAAT ATTAAGGTAC GGGAGGTACT TGGAGCGGCC GCAATAAAAT ATCTTTATTT 241 TCATTACATC TGTGTGTTGG TTTTTTGTGT GAATCGATAG TACTAACATA CGCTCTCCAT 301 CAAAACAAAA CGAAACAAAA CAAACTAGCA AAATAGGCTG TCCCCAGTGC AAGTGCAGGT 361 GCCAGAACAT TTCTCTATCG ATAGGTACCG AGCTCTTACG CGTGCTAGCC CGGGCTCGAG 421 CCAAGAAAGG CAGCGCATCG TGTCAGAAGC TGCAGACTTC ATTCCAGGGC CCCCCTCTCC 481 TGGTTTGGGG CCTGCTTTCC AATCTCAAGC CTGACCTGGG TCAGACCTGG GCAGGAGCCA 541 TGGGGAAGGC AGCTGCTCTT CTGCATAACA AAGGGTGGCC CTGCTGGAGA GCCAGGGAAC 601 ACACCAGGGA CGAGTCTTAC AATCCACTAT GGTTTCTTCA TCTATCCCCC GACTAAGATT 661 GTCTGAATCT GCAAATAAAA CCTCTAGATA CTGGTGTAAT GCAAATCCCA GGTAAACAGT 721 GACTGATATA TTGTGTAACC AAGTCCCAGA TTCAGTACGG GTTTTATTTG GCAGCCCTAC 781 CCCTGGCAGC TTGCTCAATT TTATTTCTCC AGCGCACAAA GCCACAAATT CACACTCGGG 841 TATCCCAGCT GTGTCTTGGA GAAGGCTTAG CATATTACTG GTGTATAGCC ACACACCCTT 901 GTCCCCACCC CAGCCACATG CCCCTGTCCC CAACCCAGCC ACACACCCTG TCCCCACCCC 961 AGACACACAC CCCTGGGCAG GACTTAGCAG AAAATAATTG GAGCTGTGGG AAGCCCTTCG 1021 TGGGCACCGA TCTTTAACTA TGTTGCCCCT CGTCTGAGCC GATGTACTCT GGCTCAGAGA 1081 TTGGATGGGG TAGATTTTTC AATTCTTCCT TACAGCAGAA CGAAAGATAG TTTGCTCATT 1141 GCGAGTCCAG CTTTGCAACT AGGAAGTCCC TCAGGCCTCT CAAGGTTGGG AGAAGCCCTT 1201 TCAACT //
Primer: Luc_R Sequence file: RDB10004_A9C7b.seq
>10004_10004_A9C7_3_Luc_R_B01_02_ABI08.ab1 1 TCATTGGTTC AGGGACCAGG GCGTATCTCT TCATAGCCTT ATGCAGTTGC TCTCCAGCGG 61 TTCCATCTTC CAGCGGATAG AATGGCGCCG GGCCTTTCTT TATGTTTTTG GCGTCTTCCA 121 TGGTGGCTTT ACCAACAGTA CCGGAATGCC AAGCTTGTCC GGGAAGAGGA GCCTTGGATG 181 TGTGTGTCCC TGAGGTGTCC TGAAAGGGTC TTGAGTCTGC GGAGGAATGG TACGGTACGG 241 AACAGCTTTA TAGCTGGTCC TGGGGTGGGG GGCAGGGAGG AAGAAGAGAG GTCACACCCC 301 TGGGACCTCC TGGGAGCTCA GCATGAAACA AGCCCCTGAG TCCTGAGGAG GGGCTGTCTA 361 GAAAGGACAC AAAAGCCTGT CTGGACTGAG AACATCTGGT CACCGCTGTC TGTTAGCCAT 421 ATACTTCACC ATTGACTTAA GGGTGTCTGA CCAATTAGGA CATTCAGGAC AGGGGTGGTA 481 ATAGCTCAGT GGTAGAGCAG CACCGGCCCT GCACACGTGA AGCCCTAAAT TCAATCCCAG 541 CATGGGATGG AAGGTGGGTC CTGGAGACGA CGCGGAGGTC TCACGTTCTC TGCTGGATGC 601 CTGCTCCCAG TGTCTGGAAT GTGCACCTGT AGTCTTGTCC ATCACTGCTT CTAAACCCTG 661 GAGAGAACCA GATGTCAATC AACACCTGGG CTCTCCAACT GACTTGCTTC CCAGGTGTGC 721 AAAAGACTTT TCTAGGGCTG ATAAGAGTGA GGCTTCTCCA CTTTGGGGGC TGGGGGACCT 781 TCCTTGGTTG CAAAGCCTGG GACTCGCCAA TGAGCAACCT ATCCTTTCCC TTTCTTGCTG 841 TAGGAGAATT GAAAATCTAC CCCATCCCAA TCTCTGAGCC AGAGTACATC GGCTCAGACG 901 AGGGGCAACA TAGTTAAGAT CGGTGCCCAC GAAAGGGCTT TCCACAGCTC CAATTATTTT 961 CTGCTAAGTC CTGCCCAGGG GTGTGTGTCT GGGGTGGGGA CAGGGTGTGT GGCTGGGTTT 1021 GGGGACAGGG GCATGTGGCT GGGGTGGGGA CAAGGGTGTG TGGGCTATAC ACAGTAATAT 1081 GCTAAGCCTT CTCCAAGACA CAGCTGGAAT ACCGAGTGTG AAGTTGTGCC TTTGTGCGCT 1141 GAGAATAATT GAGCAGCTGC CAAGTAGCTG ACAAGTGAAG CCTGGTATAA CG //
Primer: pGCAP_R Sequence file: RDB10004_A9C7c.seq
>10004_10004_A9C7_3_pGCAP_R_C01_03_ABI08.ab1 1 CAGTGAATCT TTATAATGTC TGCTCGAAGC GGCCGGCCGC CCCGACTCTA GAATTACACG 61 GCGATCTTTC CGCCCTTCTT GGCCTTTATG AGGATCTCTC TGATTTTTCT TGCGTCGAGT 121 TTTCCGGTAA GACCTTTCGG TACTTCGTCC ACAAACACAA CTCCTCCGCG CAACTTTTTC 181 GCGGTTGTTA CTTGACTGGC GACGTAATCC ACGATCTCTT TTTCCGTCAT CGTCTTTCCG 241 TGCTCCAAAA CAACAACGGC GGCGGGAAGT TCACCGGCGT CATCGTCGGG AAGACCTGCG 301 ACACCTGCGT CGAAGATGTT GGGGTGTTGG AGCAAGATGG ATTCCAATTC AGCGGGAGCC 361 ACCTGATAGC CTTTGTACTT AATCAGAGAC TTCAGGCGGT CAACGATGAA GAAGTGTTCG 421 TCTTCGTCCC AGTAAGCTAT GTCTCCAGAA TGTAGCCATC CATCCTTGTC AATCAAGGCG 481 TTGGTCGCTT CCGGATTGTT TACATAACCG GACATAATCA TAGGACCTCT CACACACAGT 541 TCGCCTCTTT GATTAACGCC CAGCGTTTTC CCGGTATCCA GATCCACAAC CTTCGCTTCA 601 AAAAATGGAA CAACTTTACC GACCGCGCCC GGTTTATCAT CCCCCTCGGG TGTAATCAGA 661 ATAGCTGATG TAGTCTCAGT GAGCCCATAT CCTTGCCTGA TACCTGGCAG ATGGAACCTC 721 TTGGCAACCG CTTCCCCGAC TTCCTTAGAG AGGGGAGCGC CACCAGAAGC AATTTCGTGT 781 AAATTAGATA AATCGTATTT GTCAATCAGA GTGCTTTTGG CGAAGAAGGA GAATAGGGTT 841 GGCACCAGCA GCGCACTTTG AATCTTGTAA TCCTGAAGGC TCCTCAGAAA CAGCTCTTCT 901 TCAAATCTAT ACATTAAGAC GACTCGAAAT CCACATATCA AATATCCGAG TGTAGTAAAC 961 ATTCCAAAAC CGTGATGGAA TGGAAACAAC ACTTAAAATC GCAGTATCCG GGAATGATTT 1021 GATTGCCAAA AAATAGGATC TCTGGCATGC GAGAATCTCA CGCAAGGCAG TTCTATGAGG 1081 CAGAGCGGAC CACCTTTTAG GCAAGACCAG TAGATCCAGA GGAGTTCAAT GGATCAGTGC 1141 ATGTCTGGTC CCTAATCGGA AGACTCTGGG CACAAATTCG TATCCATTAA AAACCCGGGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Nakatake, Y., Klf4 cooperates with Oct3/4 and Sox2 to activate the Lefty1 core promoter in embryonic stem cells. Mol. Cell. Biol., 26, 7772-7782 (2006). PMID 16954384.

2022.05.17

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