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pMYE3-7

Expression clone of S. cerevisiae SAR1 protein in E. coli

Catalog number RDB08882
Resource name pMYE3-7
Alternative name 342
Clone info. Expression clone of S. cerevisiae SAR1 protein in E. coli. D32G mutant.
Comment sar1-D32G (intron removed) on pET3d
Vector backbone pET3d (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae SAR1
Depositor|Developer Nakano, Akihiko |
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External Database
S. cerevisiae SAR1

          Reference sequence
            
           
          Sequence RDB08882z.seq
          Remarks, protocol and/or map (pdf) RDB08882.pdf

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (J. Biocmem. 124, 816-823, 1998).
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          提供条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biocmem. 124, 816-823, 1998)。
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          RDB08882 pMYE3-7 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pMYE3-7 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08882).

          Reference section:

          Saito, Y., Kimura, K., Oka, T., Nakano, A., Activities of mutant Sar1 proteins in guanine nucleotide binding, GTP hydrolysis, and cell-free transport from the endoplasmic reticulum to the Golgi apparatus. J. Biocmem. 124, 816-823 (1998). PMID 9756629. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content Akihiko Nakano's S. cerevisiae clones (English text)

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Saito, Y., Activities of mutant Sar1 proteins in guanine nucleotide binding, GTP hydrolysis, and cell-free transport from the endoplasmic reticulum to the Golgi apparatus. J. Biocmem. 124, 816-823 (1998). PMID 9756629. [link to RRC of NBRP]

          2023.06.19

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