Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pKSE188

Plasmid clone for production of cytosolic domain of Bos1p tagged with 6xHis in E. coli

Catalog number RDB08712
Resource name pKSE188
Alternative name 168
Clone info. Plasmid clone for production of cytosolic domain of Bos1p tagged with 6xHis in E. coli.
Comment Hisx6-BOS1 delta C (1-222) on pQE-30
Vector backbone pQE-30 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae BOS1 (1-222)
Depositor|Developer Nakano, Akihiko |
Other clones in our bank

External Database
S. cerevisiae BOS1 (1-222)

          Reference sequence
            
           
          Sequence RDB08712z.seq
          Remarks, protocol and/or map (pdf) RDB08712.pdf

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nature Struct. Mol. Biol. 12, 167-174, 2005).
          Ordering
          Order form [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Please visit Information of Request for Distribution.[link] 
          提供案内 (日本国内) [open/close]

          提供条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Nature Struct. Mol. Biol. 12, 167-174, 2005)。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB08712 pKSE188 Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pKSE188 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08712).

          Reference section:

          Sato, K., Nakano, A., Dissection of COPII subunit-cargo assembly and disassembly kinetics during Sar1p-GTP hydrolysis. Nature Struct. Mol. Biol. 12, 167-174 (2005). PMID 15665868. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content Akihiko Nakano's S. cerevisiae clones (English text)

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Sato, K., Dissection of COPII subunit-cargo assembly and disassembly kinetics during Sar1p-GTP hydrolysis. Nature Struct. Mol. Biol. 12, 167-174 (2005). PMID 15665868. [link to RRC of NBRP]

          2023.06.19

          GNP_filter3_RDBDEP_html_230615.pl