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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

316-mRFP-SED5

Plasmid clone to express mRFP-SED5 in S. cerevisiae

Catalog number RDB08659
Resource name 316-mRFP-SED5
Alternative name 115
Clone info. Plasmid clone to express mRFP-SED5 (TDH3 promoter) in S. cerevisiae.
Comment mRFP-SED5 (TDH3 promoter) on pRS316 (pCEN, URA3)
Vector backbone pRS316 (Plasmid)
Selectable markers Amp
Gene/insert name S. cerevisiae SED5
Depositor|Developer Nakano, Akihiko |
Other clones in our bank

External Database
S. cerevisiae SED5

          Reference sequence
            
           
          Sequence RDB08659z.seq
          Remarks, protocol and/or map (pdf) RDB08659.pdf

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nature 441,1007-1010, 2006).
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Please visit Information of Request for Distribution.[link] 
          Remarks
          This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Nature 441,1007-1010, 2006)。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考
          Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB08659 316-mRFP-SED5 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The 316-mRFP-SED5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08659).

          Reference section:

          Matsuura-Tokita, K., Takeuchi, M., Ichihara, A., Mikuriya, K., Nakano, A., Live imaging of yeast Golgi cisternal maturation. Nature. 441,1007-1010 (2006). PMID 16699523. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content Akihiko Nakano's S. cerevisiae clones (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB08659_A4Iop1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40 (Pr0410)
          Region: insert 5'(TDH pro)
          Sequence file: RDB08659_A4Ioc.seq check
          >08659_08659_B1KiF_M13_-40_D02_11_ABI24.ab1
              1 NNNNNNNNNG NNGNANTGGA GCTCCNCCGC GGTGGCGGCC GCTCTAGAAC TAGTGGATCC
             61 GGAGATTCCT TGATTACGTA AGGGAGTTAG AATCATTTTG AATAAAAAAC ACGCTTTTTC
            121 AGTTCGAGTT TATCATTATC AATACTGCCA TTTCAAAGAA TACGTAAATA ATTAATAGTA
            181 GTGATTTTCC TAACTTTATT TAGTCAAAAA ATTAGCCTTT TAATTCTGCT GTAACCCGTA
            241 CATGCCCAAA ATAGGGGGCG GGTTACACAG AATATATAAC ATCGTAGGTG TCTGGGTGAA
            301 CAGTTTATTC CTGGCATCCA CTAAATATAA TGGAGCCCGC TTTTTAAGCT GGCATCCAGA
            361 AAAAAAAAGA ATCCCAGCAC CAAAATATTG TTTTCTTCAC CAACCATCAG TTCATAGGTC
            421 CATTCTCTTA GCGCAACTAC AGAGAACAGG GGCACAAACA GGCAAAAAAC GGGCACAACC
            481 TCAATGGAGT GATGCAACCT GCCTGGAGTA AANNNNNNCA CAAGGCAATT GACCCACGCA
            541 TGTATCTNTC TCANTNNCNT ACACCTTCTA NTANCTTCTG CTCNCTCTGA TNNGNAAAAA
          601 NCTGANNANN NNNGNTGAAA CCANTTCCCT GANATNNNTC CCNNACNNGN NNNNNNNNN
          //
          Primer: Reverse-2 (Pr0100)
          Region: insert 3'(CMK1 ter 3')
          Sequence file: RDB08659_A4Iod.seq check
          >
          //
          Primer: Red-C_F (Pr0432)
          Region: insert mid(mRFP1 3',sed5 5')
          Sequence file: RDB08659_A4Ioe.seq check
          >08659_08659_B1KiF_Red-C_F_D01_04_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNA NNAGCGCGCC GAGGGCCGCC ACTCCACCGG CGCCAGATCT
             61 ATGAACATAA AGGATAGAAC TTCAGAATTT CAACAAAGTG TGTTGAGTTA CAAGAAAAGA
            121 AACAAAAACT TTAGAGAACA GCAGAGGGAA CGTCTACAAN AGAAAGAAAG TGAAAACTTT
            181 GCCAACAACA CAACCGGCAA TGGGAAAAGT GTCTCTGAGT TTCAAAAAAA GGCTTCTGGC
            241 ATAGCTCACN AAATTTCATC CACCGCACAA TTATTATCNA AATTANCANT GNTGGNCANN
            301 NNGAANNCGA TGTTCANTGA NNACCCTGNA NANANNGNCN ANNNNTCNNT NTTGATNNNN
          361 NNGAANANNN NNGCCNNNNN NNNNN
          //
          Primer: CMK1ter_R (Pr0015)
          Region: insert mid(CMK1 ter 5',sed5 3')
          Sequence file: RDB08659_A4Iof.seq check
          >08659_08659_A4Io_CMK1ter_R_F04_16.ab1
              1 NNNNNNNNNT NTNNNNTTNC GNNAGCACTG TGTTAACTAA TTGTGCAAAA GCTTAGATCT
             61 TTAATTGACT AAAACCCAAA TAACGAAAAA TACAAAGATT ATAAAAAAAA CCTTTGCGGC
            121 TAACCATCTA TTACTCTTTA TCCTGTCGAA GTATTTCAAA AGTTCCCTTT GAGCACCACT
            181 AATGTTTAAA TCGATGTCGT CTACGTTTGC ATCAATCCTT TGGATTACTT CGCCCTGTTC
            241 TTGAACCATG GAGGCCAGTT GTTGGAAGAG ATTTCCCACT TCCTGGATTG TGGATTCTAT
            301 TGTCTCCACC GCCCTATTTC TTTCTTGTAA GTAGACGTTA TTGGATAACT GTCCCTCTTC
            361 CATTAACATT AGTTGAGAGT CGTTTTGAGG GAAAGACAGT TCTCCTTGGT TGGACGAGTT
            421 GTTATTCTTT TCTGAGGATT CATCTAGTAG TGAGGTCATG AATGGGTTGG AGTTGTTATA
            481 CGTGGTCAAG TCCGCGGCGT GATTGCTTTG CGTTTGGTCA TCAGCTGGTG CATGTCCAGT
            541 GTCGGTGGTT AGTTTTTGCC ATCTGTCTTT GTTAGCCATT TCTAGTCGTT GCCTTTCCTC
            601 CAATACGTCT TTAAAACTTC CTGATATGTT TTTCATTTGC GTGTTTAAAA GATTTACGAC
            661 ATTTTTGGAG TGCTGTACGG CACTAGGTTG CTTAGAAGAT TGATTTGATG TGTTGCCATT
            721 CACGTCGGTT TTCTTGAGCT GACTTAATTG CACTAAACTT TGTTCGATGG CATAAATCTT
            781 CCTTTTGATC AAAAATGATA GCTCGGCNAT CTCTACAGGG TTGTCATTGA ACATCNGTTT
            841 CCTTTNGGCC NGCACTGCTA ATTTCNATNA TAATTGTGCG GNGGANGAAA TTTCGNGANC
            901 TATGCNNGAA NCNNTTTTTT TGAAACTNNG ANACNCTTTT CCNNTTGNNN GNTNNNGNNN
          961 NNGGCNAANT TTTCNCTTTN NTTCTNNNNN ANANNNNNCN NCNNNNNNNN NN
          //
          Primer: TDH3pro_F (Pr0431)
          Region: insert mid(TDH pro 3',mRFP 5')
          Sequence file: RDB08659_A4Iog.seq check
          >08659_08659_A4Io_TDH3pro_F_E04_13.ab1
              1 NNNNNNNNNN NNGNNNTGNN TGNANTTCTN TAAATCTATT TCTTAAACTT CTTAAATTCT
             61 ACTTTTATAG TTAGTCTTTT TTTTAGTTTT AAAACACCAA GAACTTAGTT TCGAATAAAC
            121 ACACATAAAC AGAATTCATG GCCTCCTCCG AGGACGTCAT CAAGGAGTTC ATGCGCTTCA
            181 AGGTGCGCAT GGAGGGCTCC GTGAACGGCC ACGAGTTCGA GATCGAGGGC GAGGGCGAGG
            241 GCCGCCCCTA CGAGGGCACC CAGACCGCCA AGCTGAAGGT GACCAAGGGC GGCCCCCTGC
            301 CCTTCGCCTG GGACATCCTG TCCCCTCAGT TCCAGTACGG CTCCAAGGCC TACGTGAAGC
            361 ACCCCGCCGA CATCCCCGAC TACTTGAAGC TGTCCTTCCC CGAGGGCTTC AAGTGGGAGC
            421 GCGTGATGAA CTTCGAGGAC GGCGGCGTGG TGACCGTGAC CCAGGACTCC TCCCTGCAGG
            481 ACGGCGAGTT CATCTACAAG GTGAAGCTGC GCGGCACCAA CTTCCCCTCC GACGGCCCCG
            541 TAATGCAGAA GAAGACCATG GGCTGGGAGG CCTCCACCGA GCGGATGTAC CCCGAGGACG
            601 GCGCCCTGAA GGGCGAGATC AAGATGAGGC TGAAGCTGAA GGACGGCGGC CACTACGACG
            661 CCGAGGTCAA GACCACCTAC ATGGCCAAGA AGCCCGTGCA GCTGCCCGGC GCCTACAAGA
            721 CCGACATCAA GCTGGACATC ACCTCCCACA ACGAGGACTA CACCATCGTG GAACAGTACG
            781 AGCGCGCCGA GGGCCGCCAC TCCNCCGGCG CCNGATCTAT GAACATAAAG GATAGAACTT
            841 CNNAATTTCN ACNAAGTGTG TTGAGTTACN AGAAAAGAAA CAAAAACTTT ANAGAANNGC
            901 ANANGGAACG TCTACNANAN AAAGNNNGTG AAAACNTTGC CNACNACNCN ACNNGNNNNG
            961 GGAAAANNGN CTCNNNNNTN NNNAAAANNN NNNNNGGNNN ANCNNNNNAN NTTNNNNNNN
          1021 NNN
          //
          Primer: phRLR1 (Pr0219)
          Region: AmpR pro,CEN_ARS
          Sequence file: RDB08659_A4Ioh.seq check
          >08659_08659_B1KiF_phRLR1_B02_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGGNT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGGGTCCTTT
             61 TCATCACGTG CTATAAAAAT AATTATAATT TAAATTTTTT AATATAAATA TATAAATTAA
            121 AAATAGAAAG TAAAAAAAGA AATTAAAGAA AAAATAGTTT TTGTTTTCCG AANATGTAAA
            181 AGACTCTAGG GGGATCGCCA ACAAATACTA CCTTTTATCT TGCTCTTCCT GCTCTCAGGT
            241 ATTAATGCCG AATTGTTTCA TCTTGTCTGT GTAGAAGACC ACACACGAAA ATCCTGTGAT
            301 TTTACATTTT ACTTATCGTT AATCGAATGT ATATCTATTT AATCTGCTTT TCTTGTCTAA
            361 NAAATATATA TGTAAAGTAC GCTTTTTGTT GAAATTTTTT AANNNTTTGT TTATTTTTTT
            421 TTCTTCATTC CGNAANTCTT CNNNNNNNTT TATTTACTTT NNANNNNNNN NNTNNNNNNC
          481 NNNNAANNNN NNNNNN
          //
          Primer: URA3pro_F (Pr0170)
          Region: URA3 pro,URA3 5'
          Sequence file: RDB08659_A4Ioi.seq check
          >08659_08659_B1KiF_URA3pro_F_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNT AACCCAACTG CACAGAACAA AAACCTGCAG GAAACGAAGA
             61 TAAATCATGT CGAAAGCTAC ATATAAGGAA CGTGCTGCTA CTCATCCTAG TCCTGTTGCT
            121 GCCAAGCTAT TTAATATCAT GCACGAAAAG CAAACAAACT TGTGTGCTTC ATTGGATGTT
            181 CGTACCACCA AGGAATTACT GGAGTTAGTT GAAGCATTAG GTCCCAAAAT TTGTTTACTA
            241 AAAACACATG TGGATATCTT GACTGATTTT TCCATGGAGG GCACAGTTAA GCCGCTAAAG
            301 GCATTATCCG CCAAGTACAA TTTTTTACTC TTCGAAGACA GAAAATTTGC TGACATTGGT
            361 AATACAGTCA AATTGCAGTA CTCTGCGGGT GTATACAGAA TAGCAGAATG GGCAGACATT
            421 ACGAATGCAC ACGGTGTGGT GGGCCCAGGT ATTGTTAGCG GTTTGAAGCA GGCGGCAGAA
            481 GAAGTAACAA AGGAACCTAN AGGCCTTTTG ATGTTAGCAG AATTGTCATG CAAGGGCTCC
            541 CTATCTACTG GANAATATAC TAAGGGTACT GTTGACNTTG CNAANNANCN ACNAANNNTT
            601 TGNTANCGNN TTNANTNGCT NNAANAGNNA TGGGNGGNAA GANANGNNNG NNNNNAATTG
            661 GTNNNNNNNN ACNCCCNNNN NNGNNNTTAN ANGNNNNNGN ANANNCNTNN NNNNCNANNN
          721 NNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Matsuura-Tokita, K., Live imaging of yeast Golgi cisternal maturation. Nature. 441,1007-1010 (2006). PMID 16699523. [link to RRC of NBRP]

          2023.06.19

          GNP_filter3_RDBDEP_html_230615.pl