icon below as well as clone information before placing your order.
Plasmid clone to express SEC12 protein tagged with mRFP under the control of TDH3 promoter in S. cerevisiae
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Other clones in our bank
External Database S. cerevisiae SEC12 Reference sequence |
| Sequence | RDB08650z.seq |
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| Remarks, protocol and/or map (pdf) | RDB08650.pdf |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions for distribution | Any commercial use of the BIOLOGICAL RESOURCE requires prior written consent of the DEPOSITOR. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Mol. Biol. Cell. 14, 3605-3616, 2003). |
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| Ordering | Order form [Credit Card Payment  ] [Bank Transfer Payment]Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word ]Please visit Information of Request for Distribution.[link] |
| Remarks | This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. |
[open/close]| 提供条件 | 営利機関の利用者もしくは商業利用を目的とする利用者は、事前にその旨を寄託者に届出し、また提供承諾書を取得することとする。利用者は研究成果の公表にあたって寄託者の指定する文献を引用する (Mol. Biol. Cell. 14, 3605-3616, 2003)。 |
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| 提供依頼 | 提供依頼書 [Word ]提供同意書 (MTA、非営利機関による非営利学術研究用)[Word ]手続きの概要は、「提供申込みについて[link]」をご覧ください。 |
| 備考 | Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 |
| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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| RDB08650 | 316-mRFP-SEC12 | DNA solution |
icon below as well as clone information before placing your order.Materials & Methods section:
| The 316-mRFP-SEC12 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08650). |
Reference section:
| Sato, K., Sato, M., Nakano, A. , Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Akihiko Nakano's S. cerevisiae clones (English text) |
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RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
| Test sheet | RDB08650_A4Io.pdf |
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Nucleotide sequence of a portion of this resource (if available).
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Primer: TDH3pro_F Sequence file: RDB08650_A4Ioa.seq
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>08650_08650_A4Io_TDH3pro_F_C04_07.ab1 1 ATAAGGACGG TAGGTATTGA TTGTAATTCT GTAAATCTAT TTCTTAAACT TCTTAAATTC 61 TACTTTTATA GTTAGTCTTT TTTTTAGTTT TAAAACACCA AGAACTTAGT TTCGAATAAA 121 CACACATAAA CAGAATTCAT GGCCTCCTCC GAGGACGTCA TCAAGGAGTT CATGCGCTTC 181 AAGGTGCGCA TGGAGGGCTC CGTGAACGGC CACGAGTTCG AGATCGAGGG CGAGGGCGAG 241 GGCCGCCCCT ACGAGGGCAC CCAGACCGCC AAGCTGAAGG TGACCAAGGG CGGCCCCCTG 301 CCCTTCGCCT GGGACATCCT GTCCCCTCAG TTCCAGTACG GCTCCAAGGC CTACGTGAAG 361 CACCCCGCCG ACATCCCCGA CTACTTGAAG CTGTCCTTCC CCGAGGGCTT CAAGTGGGAG 421 CGCGTGATGA ACTTCGAGGA CGGCGGCGTG GTGACCGTGA CCCAGGACTC CTCCCTGCAG 481 GACGGCGAGT TCATCTACAA GGTGAAGCTG CGCGGCACCA ACTTCCCCTC CGACGGCCCC 541 GTAATGCAGA AGAAGACCAT GGGCTGGGAG GCCTCCACCG AGCGGATGTA CCCCGAGGAC 601 GGCGCCCTGA AGGGCGAGAT CAAGATGAGG CTGAAGCTGA AGGACGGCGG CCACTACGAC 661 GCCGAGGTCA AGACCACCTA CATGGCCAAG AAGCCCGTGC AGCTGCCCGG CGCCTACAAG 721 ACCGACATCA AGCTGGACAT CACCTCCCAC AACGAGGACT ACACCATCGT GGAACAGTAC 781 GAGCGCGCCG AGGGCCGCCA CTCCACCGGC GCCAGATCTA TGAAGTTCGT GACAGCTAGT 841 TATAACGTCG GGTATCCTGC GTACGGTGCA AAATTTTTGA ATAACGACAC ATTACTTGTG 901 GCAGGCGGTG GAGGAGAAGG AAACAATGGC ATACCAAACA AGCTGACGGT CTTGCGCGTG 961 GATCCTACCA AAGATACTGA GAAAGGAACA GTTTCATATA TTTGAGCGAG TTTGCATTGG 1021 AAGACAACGA CGACTCTCCT ACTGCAATTG ACGCTTTCCA AGGGGTATCA TTTTTGTTTG 1081 CCTGCATGAA AATAGGCACT TAGGATACTC TAGCTAAAGG TAATAGGCCA CTTGGGAGAA 1141 ACAAAAC // |
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Primer: CMK1ter_R Sequence file: RDB08650_A4Iob.seq
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>08650_08650_A4Io_CMK1ter_R_D04_10.ab1 1 CTTTATTAAA TTTTTCCTTT TCGGCAGCAC TGTGTTAACT AATTGTGCAA AAGCTTAGAT 61 CTAGCATCAT CTATTTCTCG AAAAGTTGCG TTGGTGATCT CAAACTCTGC CCCTCCAGTA 121 TTAATAATGT TACTTTCAGT ACATAAGACT TCAGTTCCAT TCACAGAACT CGTCTCATGC 181 ACGCCATGCA CTTTTATGGA ATCTACGCTA GGTACAGATG TTTTTGTACC GTGGTTGCCA 241 AACAAAGTTG TTTGATGTAA GTCTTCATCA ACTACGTAGG GCGAAGAGAT GGTGTCTCTT 301 TTCGTTAGAA AATTGTCCTT CGCGTAATTG AAAAGCATGG AATGCAAATT GTGCTTATAG 361 GAGAACTGTA AAATGTAAGA AAGCAGCACA ATAAGGATGA AGTTGGTGAA AAATTTAGAG 421 ATTTTTTGTT TCATTGAGGT GTAGTTGGCG TAGTTAAGCG GTAATTTTAT TATGTGGATA 481 GTGTTGGCTG CCGAAACACT CGCCACATAT GTAGAGTCCG GAGAGATAGT GACCTCTGTA 541 ATGGCAAAAC TATGAGCTTG TTTGAATATT TTAGACATTG ACAGGTCTTT TAGTTTCACA 601 AGAGCTATGG AATTGTCATT ACTTGCCAGT ACCGCCAATT CACCCTTCAT GTCGACATCC 661 ATAGAAGTAA TCCCTTTGAA TCTGTTTGTC ACTTGTTTGG ATCTTAATAC GGAAGTGTTT 721 CCTGATTTGA TGCTTATTTT GGTCAGCACA ATACCTTTCC CTTTTTTTAA AGAGGCTGCT 781 ATCAATACTG TGTCATCGGC TATGAAGTTT ATTTTAGATA AACTCTAATT CTTATCAAAA 841 TCTGTTTTCC TAGCAATGCA ACTTCCAGTC ACTGTTGAAA TCACTTCCAA GCTAGAACCG 901 GGTGATATAA GCAACAACCT TACCATCAGT GGAAAAGTGT AAATCCTTTA CTTCACCCCT 961 AGTCTCGATC TCAAACTTCT CTGTCAAGTC GCTCGGGTCA ATGATTCTCA TTATAGCAGG 1021 TACTTTAGAT GATGCGATAG CTGCAACGGG TACCTCTCGG TGGAAATATA ACCAGCTCGT 1081 GTAGTTCATC CGGCATTTTG TAGAATGCGT TCAAGGTCTA ACCTAGTTGA AGAACTCATT 1141 GAATCAATTC ACTATCAATA AGTTCCACAA GAGT // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
| original | Sato, K., Rer1p, a retrieval receptor for ER membrane proteins, recognizes transmembrane domains in multiple modes. Mol. Biol. Cell. 14, 3605-3616 (2003). PMID 12972550. [link to RRC of NBRP] |
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2023.06.19
GNP_filter3_RDBDEP_html_230615.pl