RDB08376 - pCAG-mNr6a1

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pCAG-mNr6a1

Expression clone of mouse Nr6a1 cDNA

Catalog number	RDB08376
Resource name	pCAG-mNr6a1
Alternative name	Mouse Nr6a1
Clone info.	Expression clone of mouse Nr6a1 cDNA. The CA promoter derived from pCA-pA expression vector was cloned into pcDNA3 vector (Invitrogen), resulting in pcaDNA3 vector (6.5 kb). Murine Nr6a1 was amplified from a cDNA clone using PCR and cloned into the pcaDNA3.
Vector backbone	pcaDNA3 (Plasmid)
Size of vector backbone	6.5 kb
Selectable markers	Amp^r
Gene/insert name	Mouse Nr6a1 cDNA
Depositor\|Developer	TANAKA, Susumu \|

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mouse Nr6a1

External Database
Mouse Nr6a1

Reference sequence

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Biochem Biophys Res Commun, 403(2), 178-183, 2010).The availability of theBIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

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Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.(Biochem Biophys Res Commun, 403(2), 178-183, 2010).The availability of theBIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit organization for a not-for-profit research.RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Ordering	Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Please visit Information of Request for Distribution.[link]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Biochem Biophys Res Commun, 403(2), 178-183, 2010)。学術機関の学術研究に限る。利用者が本件リソースを使用して得られた研究成果に基づき特許権の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。付加的提供条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。

提供条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Biochem Biophys Res Commun, 403(2), 178-183, 2010)。学術機関の学術研究に限る。利用者が本件リソースを使用して得られた研究成果に基づき特許権の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
付加的提供条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

提供依頼

提供依頼書 [Word

]
提供同意書 (MTA、非営利機関による非営利学術研究用)[Word

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。

Catalog #	Resource name	Availability	Shipping form	Fee (non-profit org.)
RDB08376	pCAG-mNr6a1	Under QC test. Please contact us.	DNA solution	Please contact us.

Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pCAG-mNr6a1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08376).

Reference section:

Tanaka, S., Kodama, T., Nonaka, T., Toyoda, H., Arai, M., Fukazawa, M., Honda, Y., Honda, M., Mignot, E., Transcriptional regulation of the hypocretin/orexin gene by NR6A1. Biochem. Biophys. Res. Commun. 403(2), 178-183, (2010). PMID 21056546. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

Tips

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original	Tanaka, S., Transcriptional regulation of the hypocretin/orexin gene by NR6A1. Biochem. Biophys. Res. Commun. 403(2), 178-183, (2010). PMID 21056546. [link to RRC of NBRP]

2023.08.17

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