Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CS-CDF-UbCG-PRE

Lentivirus vector plasmid of GFP driven by UbC promoter.

Catalog number RDB08363
Resource name CS-CDF-UbCG-PRE
Clone info. Lentivirus vector plasmid of GFP driven by UbC promoter.
Comment Commonly requested with pCMV-VSV-G-RSV-Rev (RDB04393) and pCAG-HIVgp (RDB04394).
Selectable markers Ampicillin (E. coli). Please note that Zeo resistance marker is not included in the lentivirus produced from this vector.
Depositor|Developer Miyoshi, Hiroyuki |
Other clones in our bank

External Database

            Reference sequence
              
             
            Sequence RDB08363z.seq
            Remarks, protocol and/or map (pdf) RDB08363.pdf

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer.
            Ordering
            Order form [Credit Card Payment]  [Bank Transfer Payment]
            MTA, for use for not-for-profit academic purpose)[Word]
            Please visit Ordering instruction.[link] 
            Remarks ((Remarks)) Remember that you will be working with samples containing infectious virus.
            Please contact us. We will provide you with appropriate documents.
            提供案内 (日本国内) [open/close]

            提供条件 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。
            提供依頼
            提供依頼書 [Word]
            提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word]
            遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
            手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。
            備考 【使用上の注意】このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB08363 CS-CDF-UbCG-PRE DNA solution

            Ordering instruction of plasmids [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The CS-CDF-UbCG-PRE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08363).

            Reference section:

            Further references such as user reports and related articles (go to bottom)


            Tips

            Featured content

            Featured content Lentiviral plasmid list (English text)

            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB17485_A9I4p1-1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: EGFP-M_R (Pr0743)
            Region: EGFP,Ubc pro
            Sequence file: RDB17485_A9I4a.seq check
            >08363_17485_A9I4_3_EGFP-M_R_G08_07_ABI08.ab1
                1 NNNNNNNNNN NNNNNNNNNN GNNGGNNCTT GNNGTTNNCC TTNNATGCCG TTCTTCTGCT
               61 TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA
              121 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC
              181 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC
              241 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT
              301 CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT GGTCACGAGG GTGGGCCAGG
              361 GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT CAGCTTGCCG TAGGTGGCAT
              421 CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT TACGTCGCCG TCCAGCTCGA
              481 CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT GCTCACCATG GTGGCGACCG
              541 GTAGCGCTAA ACACGGTCCG GTCTAACAAA AAAGCCAAAA ACGGCCAGAA TTTAGCGGAC
              601 AATTTACTAG TCTAACACTG AAAATTACAT ATTGACCCAA ATGATTACAT TTCAAAAGGT
              661 GCCTAAAAAA CTTCACAAAA CACACTCGCC AACCCCGAGC GCATAGTTCA AAACCGGAGC
              721 TTCAGCTACT TAAGAAGATA GGTACATAAA ACCGACCAAA GAAACTGACG CCTCACTTAT
              781 CCCTCCCCTC ACCAGANGTC CGGCGCCTGT CGATTCNNGA NAGCCTACCC TANGNCCGAA
              841 CCCNGCGTCC TGCGACNGAN AAAAGCCTAC NGCACACCTA CCGGCAGGNG GNCCNNCCCN
              901 GCATTATAAN NCNNNCANAA CGGGTGANGT CNCGACACGA CNANGGCGCG CGCNCCCNNN
            961 NNNACNGGNG CACTGNCCNN NNNNNCCGNC NTNNCTGNNG NCCCCNNNNN NGA
            //
            Primer: PRE_rev (Pr0250)
            Region: WPRE,EGFP
            Sequence file: RDB17485_A9I4b.seq check
            >08363_17485_A9I4_3_PRE_rev_H11_23_ABI24.ab1
                1 NNNNNNNNNN NNNNNGNNNN GGANCNNCNT AGTTAAGAAT ACCNGTCAAT CTTTNNCAAA
               61 TTTTGTAATC CAGAGGTTGA TTATCGATAA GCTTGATCCC TCGAGATCCT ACTAGTAGGA
              121 TCTGAGTCCG GACTTGTACA GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA
              181 CTCCAGCAGG ACCATGTGAT CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGGT
              241 GCTCAGGTAG TGGTTGTCGG GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG
              301 GTAGTGGTCG GCGAGCTGCA CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCAC
              361 CTTGATGCCG TTCTTCTGCT TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA
              421 CTCCAGCTTG TGCCCCAGGA TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT
              481 GCGGTTCACC AGGGTGTCGC CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC
              541 GTCCTTGAAG AAGATGGTGC GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA
              601 GTCGTGCTGC TTCATGTGGT CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGGTCAGGGT
              661 GGTCACGAGG GTGGGCCAGG GCACGGGCAG CTTGCCGGTG GTGCAGATGA ACTTCAGGGT
              721 CAGCTTGCCG TANGTGGCAT CGCCCTCGCC CTCGCCGGAC ACGCTGAACT TGTGGCCGTT
              781 TACGTCGCCG TCCAGCTCGA CCAGGATGGG CACCACCCCG GTGAACAGCT CCTCGCCCTT
              841 GCTCACCATG GNGGCGACCG GTAGCGCTAA ACACGGNCCG GTCTNACAAA AAAGCCNAAA
              901 ACGGNCAGAA TTTANCGGAN NATTTACTAG TCNNNNNCTN NAANTTACNT ANNGACCCNA
            961 NNGNNTACNT TTNCNAANNN NGNCNNANAA ANNTTCNNNA A
            //
            Primer: CMV enh_F1 (Pr0652)
            Region: CMV pro,5'LTR,HIV-1psi
            Sequence file: RDB17485_A9I4c.seq check
            >08363_17485_A9I4_3_CMVenh_F1_A12_03_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN NNGCNNNGNN NNTGNCCTTA TGGGNNTTTC CTACTTGGCA
               61 GTACATCTAC GTATTAGTCA TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA
              121 TGGGCGTGGA TAGCGGTTTG ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA
              181 TGGGAGTTTG TTTTGGCACC AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC
              241 CCCATTGACG CAAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGCGCGTT
              301 TTGCCTGTAC TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC
              361 TAGGGAACCC ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG
              421 CCCGTCTGTT GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA
              481 AAATCTCTAG CAGTGGCGCC CGAACAGGGA CTTGAAAGCG AAAGGGAAAC CAGAGGAGCT
              541 CTCTCGACGC AGGACTCGGC TTGCTGAAGC GCGCACGGCA AGAGGCGAGG GGCGGCGACT
              601 GGTGAGTACG CCAAAAATTT TGACTAGCGG AGGCTAGAAG GAGAGAGATG GGTGCGAGAG
              661 CGTCAGTATT AAGCGGGGGA GAATTAGATC GCGATGGGAA AAAATTCGGT TAAGGCCAGG
              721 GGGAAAGAAA AAATATAAAT TAAAACATAT AGTATGGGCA AGCAGGGAGC TAGAACGATT
            781 CGCAGTTAAT CCTGGCCTGT TAGAAACATC AGAANGNTGT
            //
            Primer: RRE-M_R (Pr0651)
            Region: RRE,HIV-1psi
            Sequence file: RDB17485_A9I4d.seq check
            >08363_17485_A9I4_3_RRE-M_R_B12_06_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNNN GCTGTTGNGC CTCNNTAGCC CTCNNNCAAA TTGTTCTGCT
               61 GCTGCACTAT ACCAGACAAT AATTGTCTGG CCTGTACCGT CAGCGTCATT GACGCTGCGC
              121 CCATAGTGCT TCCTGCTGCT CCCAAGAACC CAAGGAACAA AGCTCCTATT CCCACTGCTC
              181 TTTTTTCTCT CTGCACCACT CTTCTCTTTG CCTTGGTGGG TGCTACTCCT AATGGTTCAA
              241 TTTTTACTAC TTTATATTTA TATAATTCAC TTCTCCAATT GTCCCTCATA TCTCCTCCTC
              301 CAGGTCTGAA GATCAGCGGC CGCTTGCTGT GCGGTGGTCT TACTTTTGTT TTGCTCTTCC
              361 TCTATCTTGT CTAAAGCTTC CTTGGTGTCT TTTATCTCTA TCCTTTGATG CACACAATAG
              421 AGGGTTGCTA CTGTATTATA TAATGATCTA AGTTCTTCTG ATCCTGTCTG AAGGGATGGT
              481 TGTAGCTGTC CCAGTATTTG TCTACAGCCT TCTGATGTTT CTAACAGGCC AGGATTAACT
              541 GCGAATCGTT CTAGCTCCCT GCTTGCCCAT ACTATATGTT TTAATTTATA TTTTTTCTTT
              601 CCCCCTGGCC TTAACCGAAT TTTTTCCCAT CGCGATCTAA TTCTCCCCCG CTTAATACTG
              661 ACGCTCTCGC ACCCATCTCT CTCCTTCTAG CCTCCGCTAG TCAAAATTTT TGGCGTACTC
              721 ACCAGTCGCC GCCCCTCGCC TCTTGCCGTG CGCGCTTCAG CAAGCCGAGT CCTGCGTCGA
            781 GAGAGCTCCT CTGGTTTCCN TTTCGCTTTC NAG
            //
            Primer: BGH_rev2 (Pr0606)
            Region: bGHp(A),3'LTR
            Sequence file: RDB17485_A9I4e.seq check
            >08363_17485_A9I4_3_BGH_rev2_G11_20_ABI24.ab1
                1 NNNNNNNNNN NNNNNNNNGG GGGNNGGNNN NNNACAGATG GCTGGCAACT AGAAGGCACA
               61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTGCTAGAG ATTTTCCACA CTGACTAAAA
              121 GGGTCTGAGG GATCTCTAGT TACCAGAGTC ACACAACAGA CGGGCACACA CTACTTGAAG
              181 CACTCAAGGC AAGCTTTATT GAGGCTTAAG CAGTGGGTTC CCTAGTTAGC CAGAGAGCTC
              241 CCAGGCTCAG ATCTGGTCTA ACCAGAGAGA CCCAGTACAG TCCGGATGCA GCTCTCGGGC
              301 CATGTGATGA AATGCTAGGC GGCTGTCAAA CCTCCACTCT AATACTTCTC TCTCCGGGTC
              361 ATCCATCCCA TGCAGGCTCA CAGGGTGTAA CAAGCGGGTG TTCTCTCCTT CATTGGCTTC
              421 TTCTACCTTC TCTTGCTCAA CTGGTACTAG CTTGTAGCAC CATCCAAAGG TCAGTGGATA
              481 TCTGATCCCT GGCCCTGGTG TGTAGTTCTG CCAATCAGGG AAGTAGCCTT GTGTGTGGTA
              541 GATCCACAGA TCAAGGATAT CTTGTCTTCG TTGGGAGTGA ATTAGCCCTT CCAGTCCCCC
              601 CTTTTCTTTT AAAAAGTGGC TAAGATCTAC AGCTGCCTTG TAAGTCATTG GTCTTAAAGG
              661 TACCTGAGGT GTGACTGGAA AACCCACCTC CTCCTCCTCT TGTGCTTCTA GCCAGGCACA
              721 ATCAGCATTG GTAGCTGCTG TATTGCTACT TGTGATTGCT CCATGTTTTT CTAGGTCTCG
            781 ACNGTATCGA TGCGGGGAN
            //
            Primer: pAxEF R2 (Pr0059)
            Region: SV40p(A),Zeo,SV40pro_ori
            Sequence file: RDB17485_A9I4f.seq check
            >08363_17485_A9I4_3_pAxEFR2_C12_09_ABI24.ab1
                1 NNNNNNNNNN NNNNTNNTTT GTGNANTTGT GNNGCTATTG CTTTATTTGT AACCATTATA
               61 AGCTGCAATA AACAAGTTGG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG
              121 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC
              181 GGTGGAATCG AAATCTCGTA GCACGTGTCA GTCCTGCTCC TCGGCCACGA AGTGCACGCA
              241 GTTGCCGGCC GGGTCGCGCA GGGCGAACTC CCGCCCCCAC GGCTGCTCGC CGATCTCGGT
              301 CATGGCCGGC CCGGAGGCGT CCCGGAAGTT CGTGGACACG ACCTCCGACC ACTCGGCGTA
              361 CAGCTCGTCC AGGCCGCGCA CCCACACCCA GGCCAGGGTG TTGTCCGGCA CCACCTGGTC
              421 CTGGACCGCG CTGATGAACA GGGTCACGTC GTCCCGGACC ACACCGGCGA AGTCGTCCTC
              481 CACGAAGTCC CGGGAGAACC CGAGCCGGTC GGTCCAGAAC TCGACCGCTC CGGCGACGTC
              541 GCGCGCGGTG AGCACCGGAA CGGCACTGGT CAACTTGGCC ATGGTTTAGT TCCTCACCTT
              601 GTCGTATTAT ACTATGCCGA TATACTATGC CGATGATTAA TTGTCAACAC GTGCTGATCA
              661 GATCCGAAAA TGGATATACA AGCTCCCGGG AGCTTTTTGC AAAAGCCTAG GCCTCCAAAA
            721 AAGCCTCCTC ACTACTTCTG GAATAGCTCA GANGCAGANG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            user_report Kayamori, K., DHODH inhibition synergizes with DNA-demethylating agents in the treatment of myelodysplastic syndromes. Blood Adv. 5 (2): 438-450 (2021). PMID 33496740. [link to RRC of NBRP]
            user_report Zhong, C., Efficacy of the novel tubulin polymerization inhibitor PTC-028 for myelodysplastic syndrome. Cancer Sci. 111 (12): 4336-4347 (2020). PMID 33037737. [link to RRC of NBRP]

            2024.03.26

            HTML_filter_RDB_240326.pl