Please review the QC test results indicated by icon below as well as clone information before placing your order.

pET25b_Tr-egl2-His

Expression vector of Endoglucanase EG-II of Trichoderma reesei

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17893_B0A6p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-B_F (Pr0723) Region: T7 pro, lacO, RBS, pelB signal, insert5' Sequence file: RDB17893_B0A6a.seq
>08309_17893_B0A6_1_pET-B_F_A07_01_ABI24.ab1     1 NNNNNNNNNN NNGNNNNGCC GGCNNCGATG CGTCCGGCGT ANAGGNNCGA GATCTCGATC    61 CCGCGAAATT AATACGACTC ACTATAGGGG AATTGTGAGC GGATAACAAT TCCCCTCTAG   121 AAATAATTTT GTTTAACTTT AAGAAGGAGA TATACATATG AAATACCTGC TGCCGACCGC   181 TGCTGCTGGT CTGCTGCTCC TCGCTGCCCA GCCGGCGATG GCCATGGATA TCGGAATTAA   241 TTCGGATCCG AATTCGAGCT CCGTCGACCA GCAGACTGTC TGGGGCCAGT GTGGAGGTAT   301 TGGTTGGAGC GGACCTACGA ATTGTGCTCC TGGCTCAGCT TGTTCGACCC TCAATCCTTA   361 TTATGCGCAA TGTATTCCGG GAGCCACTAC TATCACCACT TCGACCCGGC CACCATCCGG   421 TCCAACCACC ACCACCAGGG CTACCTCAAC AAGCTCATCA ACTCCACCCA CGAGCTCTGG   481 GGTCCGATTT GCCGGCGTTA ACATCGCGGG TTTTGACTTT GGCTGTACCA CAGATGGCAC   541 TTGCGTTACC TCGAAGGTTT ATCCTCCGTT GAAGAACTTC ACCGGCTCAA ACAACTACCC   601 CGATGGCATC GGCCAGATGC AGCACTTCGT CAACGACGAC GGGATGACTA TTTTCCGCTT   661 ACCTGTCGGA TGGCAGTACC TCGTCAACAA CAATTTGGGC GGCAATCTTG ATTCCACGAG   721 CATTTCCNAG TATGATCAGC TTGTTCAGGN NTGCCTGTCT CTGGGCGCAT ACTGCATCGT   781 CGACATCCAC AATTATGCTC GATGGAACGG NGNNATCATT GGTCAGGGCG GCCCTACTAA   841 TGCTCNATTC ACGANCCTTN GNNCGCANTT GGCATCAAAG TACGCATCTC ANNCNANGGN   901 GTGGTTCNGN ATCNTGAATG ANCCCNNCGA NNNNNNNNTC NNNNCCNNGG NNNNNNCGNN 961 NNNNNANNNN NNNNNN //
Primer: f1ori_R3 (Pr0142) Region: T7 ter, 6xHis, HSV tag, insert3' Sequence file: RDB17893_B0A6b.seq
>08309_17893_B0A6_1_f1ori_R3_B07_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNCCTTTNN GCNNAAAACC CCTCNAGANC CGTTTAGAGG    61 CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC AGCCAACTCA GCTTCCTTTC   121 GGGCTTTGTT AGCAGCCGGA TCTCAGTGGT GGTGGTGGTG GTGCTCGACA TCCTCGGGGT   181 CTTCCGGGGC GAGTTCTGGC TGGCTAGCCC GTTTGATCTC GAGTGCGGCC GCAAGCTTGT   241 CGACCTTTCT TGCGAGACAC GAGCTGACCA AGGATGTGTC CGTCCATGAG TTACCACTGC   301 CAGTCGGTGT TTCCGTCAGG ACATACGTGC TATCAAATGA TCCGGCACCC CAACCAACAT   361 AGCCAAGATA GACATCTGAG TTCTGGTTGA GATATTGGAT TTGCTGGCAC ATGTCTTGTA   421 TGCAGGACTG AACGTTGCCA CCACCGGTTT CTGTCAGGAT AGCCTGGCGA TTGTTCTGTC   481 GGAGCCAAGT GGCAAGCGGA GAAAAGGCGC CGTCAATGTT ATTTGTAGTA CATTCGGCGT   541 GAGTACCGGA GTTGTCTGAG TCCAAGTATT TGTGCACGTC AAAAATCAGA TTCGTTGTTG   601 ACCCATCCGG GTTCGTGACT TGAGACAGGG CGGCTGCACT GCCATCGGAT ATGAAAGCCC   661 CAGCAGATTG CCAATCATTT CNAGGCAAAG AGATGAATTG CGACGTAGCA CCAGCGTTGC   721 GGATTGCGGT TACAACCTCT TGGANCGTGG CAGCCCANGT GTTGATGTTC ACGTCGTNNN   781 GCTCATTCAT GATGCCGAAC CACACCCTCG ACTGANATGC GTACTTTGAT GNCAACTNCN   841 ANCAAANGNT CGNGANTTGA NCATTAGTAN GGCCGCCCNN ANCAATGATN CCACCGNTNC   901 NTCNANCNTA ATNNNNNNTG TCGANNANGC ANTATGCNNC CANNNNNAGG NNNNNNNNNA 961 NNNANNNNAN NNNNNNNNN //
Primer: lacIpro_F2 (Pr0647) Region: lacI gene Sequence file: RDB17893_B0A6c.seq
>08309_17893_B0A6_1_laclpro_F2_C07_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNGNNG GNNNCGCATA AGGGNNANCG TCGAGNTCCC GGNNACCATC    61 GAATGGCGCA AAACCTTTCG CGGTATGGCA TGATAGCGCC CGGAAGAGAG TCAATTCAGG   121 GTGGTGAATG TGAAACCAGT AACGTTATAC GATGTCGCAG AGTATGCCGG TGTCTCTTAT   181 CAGACCGTTT CCCGCGTGGT GAACCAGGCC AGCCACGTTT CTGCGAAAAC GCGGGAAAAA   241 GTGGAAGCGG CGATGGCGGA GCTGAATTAC ATTCCCAACC GCGTGGCACA ACAACTGGCG   301 GGCAAACAGT CGTTGCTGAT TGGCGTTGCC ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG   361 CAAATTGTCG CGGCGATTAA ATCTCGCGCC GATCAACTGG GTGCCAGCGT GGTGGTGTCG   421 ATGGTAGAAC GAAGCGGCGT CGAAGCCTGT AAAGCGGCGG TGCACAATCT TCTCGCGCAA   481 CGCGTCAGTG GGCTGATCAT TAACTATCCG CTGGATGACC AGGATGCCAT TGCTGTGGAA   541 GCTGCCTGCA CTAATGTTCC GGCGTTATTT CTTGATGTCT CTGACCAGAC ACCCATCAAC   601 AGTATTATTT TCTCCCATGA AGACGGTACG CGACTGGGCG TGGAGCATCT GGTCGCATTG   661 GGTCACCAGC AAATCGCGCT GTTAGCGGGC CCATTAAGTT CTGTCTCGGC GCGTCTGCGT   721 CTGGCTGGCT GGCATAAATA TCTCACTCGC AATCAAATTC AGCCGATAGC GGAACGGGAA   781 NGCGANTGGA GTGCCATGTC CGGTTTTCAA CAAACCATGC AAATGCTGAA TGANGGCATC   841 GTTCCNNCTG CNATGCTGGT TGCCNACGAT CAGATGGCGC NNGGCGCANT GCGCGCCATT   901 ANNNANNNCG GGNNGCNCGT TGGNNCNGNN NATNNCNNNA NNNGGNNACN ANNANNCCGN 961 ANNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.18

GNP_filter3_RDBDEP_html_230702.pl