Please review the QC test results indicated by icon below as well as clone information before placing your order.

pET21c_Tr-egl2-His

Expression vector of Endoglucanase EG-II of Trichoderma reesei

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17894_B0A6p1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-B_F (Pr0723) Region: T7 pro, lacO, RBS, T7 tag, insert5' Sequence file: RDB17894_B0A6a.seq
>08289_17894_B0A6_1_pET-B_F_B08_05_ABI24.ab1     1 NNNNNNNNNN NNNNGNNNNG CCGGCNNCGA TGCGTCCGGC GTAGAGGNNC GAGNNCTCGA    61 TCCCGCGAAA TTAATACGAC TCACTATAGG GGAATTGTGA GCGGATAACA ATTCCCCTCT   121 AGAAATAATT TTGTTTAACT TTAAGAAGGA GATATACATA TGGCTAGCAT GACTGGTGGA   181 CAGCAAATGG GTCGGATCCG AATTCGAGCT CCGTCGACCC AGCAGACTGT CTGGGGCCAG   241 TGTGGAGGTA TTGGTTGGAG CGGACCTACG AATTGTGCTC CTGGCTCAGC TTGTTCGACC   301 CTCAATCCTT ATTATGCGCA ATGTATTCCG GGAGCCACTA CTATCACCAC TTCGACCCGG   361 CCACCATCCG GTCCAACCAC CACCACCAGG GCTACCTCAA CAAGCTCATC AACTCCACCC   421 ACGAGCTCTG GGGTCCGATT TGCCGGCGTT AACATCGCGG GTTTTGACTT TGGCTGTACC   481 ACAGATGGCA CTTGCGTTAC CTCGAAGGTT TATCCTCCGT TGAAGAACTT CACCGGCTCA   541 AACAACTACC CCGATGGCAT CGGCCAGATG CAGCACTTCG TCAACGACGA CGGGATGACT   601 ATTTTCCGCT TACCTGTCGG ATGGCAGTAC CTCGTCAACA ACAATTTGGG CGGCAATCTT   661 GATTCCACGA GCATTTCCAA GTATGATCAG CTTGTTCAGG GGTGCCTGTC TCTGGGCGCA   721 TACTGCATCG TCGACATCCA CAATTATGCT CGATGGAACG GTGGGATCAT TGGTCAGGGC   781 GGCCCTACTA ATGCTCAATT CACGANCCTT TGGTCGCAGT TGGCATCAAA GTACGCATCT   841 CAGTCGAGGG NGTGGTTCGG CATCATGAAT GAGCCCCACN ANNNGAACAT CAACANCNNN   901 NNNGCCACNG NCCNNNANGT TGTAANCGCN NNCCGCNNCG CNGNNNCTAN NNCNNANNNN 961 NNNNNN //
Primer: f1ori_R3 (Pr0142) Region: T7 ter, 6xHis, insert3' Sequence file: RDB17894_B0A6b.seq
>08289_17894_B0A6_1_f1ori_R3_C08_08_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNT NNNCCTTTNN GCNANNAACC CCTCAAGANC CGTTTAGAGG    61 CCCCAAGGGG TTATGCTAGT TATTGCTCAG CGGTGGCAGC AGCCAACTCA GCTTCCTTTC   121 GGGCTTTGTT AGCAGCCGGA TCTCAGTGGT GGTGGTGGTG GTGCTCGAGT GCGGCCGCAA   181 GCTTGTCGAC CTTTCTTGCG AGACACGAGC TGACCAAGGA TGTGTCCGTC CATGAGTTAC   241 CACTGCCAGT CGGTGTTTCC GTCAGGACAT ACGTGCTATC AAATGATCCG GCACCCCAAC   301 CAACATAGCC AAGATAGACA TCTGAGTTCT GGTTGAGATA TTGGATTTGC TGGCACATGT   361 CTTGTATGCA GGACTGAACG TTGCCACCAC CGGTTTCTGT CAGGATAGCC TGGCGATTGT   421 TCTGTCGGAG CCAAGTGGCA AGCGGAGAAA AGGCGCCGTC AATGTTATTT GTAGTACATT   481 CGGCGTGAGT ACCGGAGTTG TCTGAGTCCA AGTATTTGTG CACGTCAAAA ATCAGATTCG   541 TTGTTGACCC ATCCGGGTTC GTGACTTGAG ACAGGGCGGC TGCACTGCCA TCGGATATGA   601 AAGCCCCAGC AGATTGCCAA TCATTTCCAG GCAAAGAGAT GAATTGCGAC GTAGCACCAG   661 CGTTGCGGAT TGCGGTTACA ACCTCTTGGA CCGTGGCAGC CCAGGTGTTG ATGTTCACGT   721 CGTGGGGCTC ATTCATGATG CCGAACCACA CCCTCGACTG ANNTGCGTAC TTTGATGCCA   781 ACTGCGACCA AAGGCTCGTG AATTGAGCAT TAGTANGGCC GCCCTGANCA ATGNTCCCAC   841 CNNNCCATCG AGCATAATTG TGGATGTCGA CGATGCAGTA TGCGCCCAGA NANANNNACC 901 CNNNNNNAAN CNNNTCATAN NTNNAAATGN NCNNNNNNTN NNNNNNNNN //
Primer: lacIpro_F2 (Pr0647) Region: lacI gene Sequence file: RDB17894_B0A6c.seq
>08289_17894_B0A6_1_laclpro_F2_D08_11_ABI24.ab1     1 NNNNNNNNNN NNNGNNGGNN NCGCATAAGG GANANCGTCG ANNTCCCGGN NACCATCGAA    61 TGGCGCAAAA CCTTTCGCGG TATGGCATGA TAGCGCCCGG AAGAGAGTCA ATTCAGGGTG   121 GTGAATGTGA AACCAGTAAC GTTATACGAT GTCGCAGAGT ATGCCGGTGT CTCTTATCAG   181 ACCGTTTCCC GCGTGGTGAA CCAGGCCAGC CACGTTTCTG CGAAAACGCG GGAAAAAGTG   241 GAAGCGGCGA TGGCGGAGCT GAATTACATT CCCAACCGCG TGGCACAACA ACTGGCGGGC   301 AAACAGTCGT TGCTGATTGG CGTTGCCACC TCCAGTCTGG CCCTGCACGC GCCGTCGCAA   361 ATTGTCGCGG CGATTAAATC TCGCGCCGAT CAACTGGGTG CCAGCGTGGT GGTGTCGATG   421 GTAGAACGAA GCGGCGTCGA AGCCTGTAAA GCGGCGGTGC ACAATCTTCT CGCGCAACGC   481 GTCAGTGGGC TGATCATTAA CTATCCGCTG GATGACCAGG ATGCCATTGC TGTGGAAGCT   541 GCCTGCACTA ATGTTCCGGC GTTATTTCTT GATGTCTCTG ACCAGACACC CATCAACAGT   601 ATTATTTTCT CCCATGAAGA CGGTACGCGA CTGGGCGTGG AGCATCTGGT CGCATTGGGT   661 CACCAGCAAA TCGCGCTGTT AGCGGGCCCA TTAAGTTCTG TCTCGGCGCG TCTGCGTCTG   721 GCTGGCTGGC ATAAATATCT CACTCGCAAT CAAATTCAGC CGATAGCGGA ACGGGAANGC   781 GACTGGAGTG CCATGTCCGG TTTTCAACAA ACCATGCNNN TGCTGAATGA NGGCATCGTT   841 CCCACNNCGA TGCTGGTTGN NNACGATCAG ATGGCGCTGG GCGCAATGCG CGCNNTTACC   901 NANNCCGGGC NGCNCGTTNG NGCNNNTATC TCNGNANNGG NANACNANNA NACCNNNNNN 961 NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.08.18

GNP_filter3_RDBDEP_html_230702.pl