RDB07871 - pGL3-AID R4 del.-17

Reporter construct of mouse Aicda locus, position -9226 to -8357 plus -8243 to -7585.

Distribution information

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nat. Immunol., 11, 148-154 (2010))。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。　学術機関の学術研究に限る。　営利機関の利用希望者は、事前に利用条件等につき寄託者と合意し、提供承諾を得ること。　利用者が本件リソースを使用して得られた研究成果に基づき特許等の申請、及び事業活動を行う場合は、寄託者と別途協議を行う。
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References and tips

Sequence information

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07871	pGL3-AID R4 del.-17	DNA solution

original	Tran, T.H., B cell-specific and stimulation-responsive enhancers derepress Aicda by overcoming the effects of silencers. Nat. Immunol., 11, 148-154 (2010). PMID 19966806.

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check

icon as well as clone information before placing your order.

Primer: pGL4-4174F Sequence file: RDB12850_A4Gia.seq
>07871_12850_A4Gi_3_pGL4-4174F_F04_16.ab1 1 GGTGGGTCAG ACATTTCTCT ATCGATAGGT ACCCACCACT CTCGGCTGTT CTGGAACTCT 61 CTGTAACCTC GGGCTGTATG AGTGTATGTG AGTGTGTGTA TATGGGTGTG GGGGGGGTGA 121 GAGTATATGA GTGTATGTGA GTGTGTATGT ATGGGTGTGA GGTGTGTGTG TGTGTATGAG 181 TGTGTGTATG AGTGTGTGTA TGAGTGTGTG TGAGTGTGTG TATATGGGGG GGTGAGG //
Primer: Luc_R Sequence file: RDB12850_A4Gib.seq
>07871_12850_A4Gi_3_Luc_R_G04_19.ab1 1 CATGATTCAG GACCAGGGCG TATCTCTTCA TAGCCTTATG CAGTTGCTCT CCAGCGGTTC 61 CATCTTCCAG CGGATAGAAT GGCGCCGGGC CTTTCTTTAT GTTTTTGGCG TCTTCCATGG 121 ATCGGTCTCC AGCGTGACTT TCTTGACTCC CTCAAAGTCT TAGGCCAAAG GAAGCTTTTC 181 TTCAAGCAAG GCTGCTTCAG TGCTGTTGTG TGACAGAGCA CTGGCCCCTG GCATTCAAAT 241 CTGAGCTTGC TCTTGTGGGC TCCTCCCCCA CGACAGCATC ACTACCACCA GGTATCCAAC 301 ATCTGCCTCC TGGGTTGGGT CCAGTAAAGA TGGGCGGGGC TGTGGTGGGC AAAGCCACAC 361 GGATTAAATG ATTGGGGGGG GGGGCCAGTT CTGCTCATGC TTAATGTCCA ACTTCCTTCG 421 AGGCTGTTCC TCTAAACTCG AGCTGTAGCA CACGCACACG CACGCCCACA CCCTCTCTTA 481 CAGATGCCGT ACACATGCCA TAGGAACAAA TACAAAACCC TTGATTTGTA AGAAAGCCAA 541 ATTCTCTCAT TTGCCTTCTT CTAAAGGAAG GAAAGAGATC TCACTTAGTT ACAAAAACCA 601 TTTTCCAGTC CCGGGAAATT TGGAACTGGT ACAGCGGCAA AGCAGTTTAC AGGGTTTTGA 661 TCAACTTTTA TTTTCTTCAT TAACTTCTCT TTCCCCTCAT TAACTTCTAT TGAAAAACCA 721 CAAGCCAACT TGGTCACATT GTTTTTAACC AAAAATACTG TGACTTGGAA ATCGAGTCGC 781 CTGCGGCTGA GCTGAGTTTT GGACATCTGG AGTTCACCAC CTCGCTTTCC CTATGAGACA 841 CACAGGTGAG GTTTCGTTAT GGCCAGCATG AGGGGCTGGC GGTCTCTGGA AAGCCTCGCG 901 GTGGGGGGCC TGCATTCTTG GTGTCTCAAG AGCCAGCACA CTCAGACATG ACTCCTCGAG 961 CCCGGACTAG CACGGCGTAA GAGCTCGGTA CCAACTTCTG CATTCCCGGA TTTTTTGTTT 1021 TTTCTCACTT TGTGTCCCTG CAGGTACTGG CATCCCTTCT GGGTATGGAA AGGCATGGAC 1081 AGTCCTCGAT GACCGGAATT ACTCAGCTTC TGCCTGATGA GCCTGC //
Primer: R4del-M_R2 Sequence file: RDB12850_A4Gic.seq
>RDB07871_12850_A4Gi_R4del-M_R2_3_B01_04.ab1 1 CAGGCGGGCG GTCTCTGGAG CCTCGGGGGG GGGGGGGGGC TGCATTCCTG TGTCTCAAAG 61 CCAGCACACT CAGACATGAC TCCTCGAGCC CGGGCTAGCA CGCGTAAAAG CTCGGTACCA 121 ACTTCTGCAT TCCCCGATTT TTGTTTTTCT CACTTTGTGG CCCTGGAGGT TACTGCATCC 181 CTCTGGGGTA TGAAAAGCCA TGGGACAGCC CTCAAACACA GACCAAAATT ACTCAGCTTC 241 TGCTGTTGGG CTGCCCAGGA CATTTCAAAG ACCAAAGAAC TGTAAACAGG TATTTATCAA 301 AAGCTTAGGT CTACGGGGAG GTGGAGGCAG GAGGATTGAG AGTTCAAGGC CATCTTCTGC 361 CTCGTGTTGA ATTCAAGGCC AGCAGGCAGG TTTGAAAGAA AAAAAAAATT TTCAAATGGA 421 ATACTATGAA ATTAAAAGGC TTCAAACTGA GTGTGGTGAC TAATCCTAGC AGCCTGGAGG 481 TTGAGGCAGG AGGATTGTCA AGAGTTGGAA ACCACCTTGA GCTCCATCCC AGGACCCACA 541 TGGTAGGGGG AGTGAACCAA TTCATGCAAG TTGTCCACTG ACCTCTACGC TCATACACAC 601 ACACACTCAT ACACACACCC ATAATACACA CTCACACACA CTCATACACA CACCCATACA 661 CACACTCATA CACACACACA CCCACACACA CACCCATACA TACACACTCA CATATACTCA 721 TACACCCTCA TCCCCACACC CATATACACA CACTCACACA CACTCATACA CACACTCATA 781 CACACACACC TCACACCCAT ACATACACAC TCACATACAC TCATATACCC TCACCCCCCC 841 CATATACACA CACTCACACA CACTCATACA CACACTCATA CACACACTCA TACACACACA 901 CACACCTCAC ACCCATACAT ACACACTCAC ATACACTCAT ATACTCTCAC CCCCCCCACA 961 CCCATATACA CACACTCACA TACACTCATA CAGCCCGAGG GTTACAGAGA GTTTCCAGAT 1021 CAGCCGAGAG TTGGTGGGTT ACCTATCGAT AAGAGAAATG TTCTGGCACC TTGCACTTGG 1081 CACTGAGACA GCTATTTTGC TAGTGTTGTT CGTTTTGTTT GATGGAAGCG ATGTTG //