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pGL4-phHIF1A

Promoter collection, Human HIF1A promoter

Catalog number RDB07693
Resource name pGL4-phHIF1A
Alternative name U3136-6
Clone info. PCR amplified human HIF1A promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 61694082 to 61695550(NC_000014.9); relatively -1431 to +38, where +1 corresponds to 1 nt of NM_001530.4.
Comment PCR cloning, forward primer: cccatggttcttttcaagaca; reverse primer: atcctcctgtcccctcagac. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor)

          Reference sequence
            
           
          Remarks, protocol and/or map (pdf) RDB07693.pdf

          Distribution information

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          Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions:
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB07693 pGL4-phHIF1A DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pGL4-phHIF1A was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07693).

          Reference section:

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)
          Featured content HIF-1 signaling pathway (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07693_A4La.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07693_A4Laa.seq check
          >07693_07693_A4La_pGL4-4174F_A04_01.ab1
              1 ATGGGTCAGA CATTTCTCTG GCCTACTGGC CGGTACCTGA GCTCGCTAGC CTCGAGGATC
             61 CCATGGTTCT TTTCAAGACA TAATACATAT CATTTAGTGT TGTAGATCTG AAAAAACAAA
            121 AGTAGCGTGA AGATCAAAAA TTTTCTAAAG AGACGGAGTC TCGCTACGTT CCCTAGGCTG
            181 GAACACCCAG GCTTCTCCAG CCTCACACCT CTGAGTAGCT GGAACCACCC TGTCCGCTAA
            241 GGTCAATGTT TAATCGTATC TTTGTAGGTC TACTGACCAG TTAAAAAGAG GTGCTGTATA
            301 CATTGGTTGT TGTCTTGTCA GAGTTTGATG CTTCTATATA GACCATTGTT TTTACATGCT
            361 AATACAATTG AAAGCCACTA CAGATATTTA TATTTACAAC CCAAAGCTAG GTTTTAACAA
            421 GAAACTCATA AGGCAAAGGT GAGAAGTAAA ATAATTTAGC GCCAAGTGGA GATATATGTG
            481 CAATGCTACT TTGTTGGGCT CAAAACATAT TTTTCTTTTA GAAGACTGAC AGGCTTGAAG
            541 TTTATGCCTC CAAAGACAAA AGTGATTACG TTTTGTTTAG TAGCTTGCAA AGTTGCCAAA
            601 GGCCATTTTT TCTACTCTTT CCCTGAAATT GGTTTATATG CTTATTAAAG TCATTTATAC
            661 CTATTTGCAA ATGCTTAACA TAGTTTCAGA TTTTAAGATT TCCCTGCAAC TTTATTTCCC
            721 TTGAAGTTTA CAGCAACAGG AGTTCATTTT TATTTTTAAT TGCATTTATT CAGTAAGTAA
            781 ACTCCGCCAC AGAAAAACTT AGTAGACAAG GTGAGTTCCC CTGTGCTCCG TGGCAAAGAG
            841 TGCGGTGGGT GACATTGACC CATGGTTAGG TAATCTGGTA AGGAAGACCC CGTTGTAACA
            901 CATCTGAGCA ACGAGACCAA AGGAAGGGCT TGCTGCCACG AGGCGAAGTC TGCTTTTTTT
            961 GAACAGAGAG CCCAGCAGAG TTGGGCGGCA ATCGTGCCCA GCACTGAGGC CGAGGAGAAG
           1021 AGAGCAGGAG CATTACATTA CTGCACCAAG AGTAGGAAAA TATGATGCAT GTTTGGGACC
           1081 AGGCAACCGA AATCCCTTCT CAGCAGCGGC TTCCTAAAGG TGGCAACGAC GTTCCTTCGG
          1141 AGAAGGCGCA GAAGTTCCCC CAGAGAAACG TCG
          //
          Primer: pGL4-136R
          Sequence file: RDB07693_A4Lab.seq check
          >07693_07693_A4La_pGL4-136R_B04_04.ab1
              1 GACTATGTTT GGCATCTTCC ATGGTGGCTT TACCAACAGT ACCGGATTGC CAAGCTTGGC
             61 CGCCGAGGCC AGATCTTGAT ATCCTCCTGT CCCCTCAGAC GAGGCAGCAC TGTGCACTGA
            121 GGAGCTGAGG CAGCGTCAGG GGGCGGGCAA GGGCGGAGGC GCGCTCGGGC GCGCGGGGAG
            181 GGGAGAGGGG GCGGGAGCGC GCCCGCTCGC CTGCCCTGGC CGGCGCGCGC TCCCGCAAGC
            241 CCGCCTCACC TGAGGTGGAG GCGGGTTCCT CGAGATCCAA TGGCGAGCGA CGCAGGCGGC
            301 AAGTCCCCAC CCCAGCCCAA GCCGCTCTCA GCCAATCAGG AGGCGGTCAG CTGAGGCACA
            361 GCTGGGACGG GCTGCGACGC TCACGTGCTC GTCTGTGTTT AGCGGCGGAG GAAAGAGAAA
            421 GGAGATGGGG GTGCGGCTCA GCCCGAGTCT GGGGACTCTG CGCCTTCTCC GAAGGAAGGC
            481 GGTGCCCGGC TTTGGGAGGC GCTGCTGAGA AGGGATTTCG GTTGCCTGGT CCCAAACATG
            541 CATCATATTT TCCTACTCTT GGTGCAGTAA TGTAATGCAC CTGCTCTCTT TCTCCTCGGC
            601 CTCAGTGCTG GGCACGATTG CCGCCCAACT CTGCTGGGCT CTCTGTTCAA AAAAGCAGAC
            661 TTCGCCTCGT GGCAGCAAGC CCTTCCTTTG GTCTCGTTGC TCAGATGTGT TACAACGGGG
            721 TCTTTCCTTA CCAGATTACC TAACCATGGG TCAATGTCAC CCACCGCACT CTTTGCCACG
            781 GAGCACAGGG GAACTCACCT TGTCTACTAA GTTTTTCTGT GGCGGAGTTT ACTTACTGAA
            841 TAAATGCAAT TAAAAATAAA AATGAACTCC TGTTGCTGTA AACTTCAAGG GAAATAAAGT
            901 TGCAGGGAAA TCTTAAAATC TGAAACTATG TTAAGCATTT GCAAATAGGT ATAAATGACT
            961 TTAATAAGCA TATAAACCCA ATTTCAGGGA AAGAGTAGAA AAAATGGCCC TTTGGCAACT
           1021 TTGCCAGCTA CTAAACAAAA CGTAATCACT TTTGTCTTGA GGCATAACTT CAAGCCTGTC
           1081 AGGTCTTCTA GAGGAAAAAT ATGTTTTGAG CCACCAAAGG TAGCATTGCA CATATATCTT
          1141 CCACCTTGCG CTAAATACTT ACTTCGTCAC GCTTTGGACC TTTATTTGA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user_report Chiba, R., ALK signaling cascade confers multiple advantages to glioblastoma cells through neovascularization and cell proliferation. PLoS One 12 (8): e0183516 (2017). PMID 28837676. [link to RRC of NBRP]

          2023.11.09

          GNP_filter3_RDBDEP_html_231027.pl