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pGL4-phSOD1

Promoter collection, Human SOD1 promoter

Catalog number RDB07686
Resource name pGL4-phSOD1
Alternative name U2950-3
Clone info. PCR amplified human SOD1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 31658211 to 31659681(NC_000021.9); relatively -1411 to +60, where +1 corresponds to 1 nt of NM_000454.4.
Comment PCR cloning, forward primer: gaaatctgccgaacttcctg; reverse primer: ccgtctccgcgactacttta. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human superoxide dismutase 1, soluble genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human superoxide dismutase 1, soluble
          Reference sequence
            
           
          Remarks, protocol and/or map (pdf) RDB07686.pdf

          Distribution information

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          Additional terms and conditions:
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB07686 pGL4-phSOD1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pGL4-phSOD1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07686).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          Tips

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07686_A4Lm.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB07686_A4Lma.seq check
          >07686_07686_A4Lm_pGL4-4174F_G07_19.ab1
              1 GGTGGGTCAG ACATTTCTCT GGCCTACTGG CCGGTACCTG AGCTCGCTAG CCTCGAGGAT
             61 GAAATCTGCC GAACTTCCTG GAACCCAAAG AAACTTTAGC CTTGGGCAAA GGCCCTTTGG
            121 CCAGCATTTG CACTGTTTAT GCAACCGTTT AGAATATACG AATTATCTGG AGACTACTAC
            181 CAAATACAAC AGGCAAAACT GCAAATATGT ATACTTCCTA GAGGATGATA AAAAAATGTG
            241 AATTGTATTT CTCTGATAGA GGATGCATTA GAGTCTGAGG GTCTAAATAG CGTAAATAAT
            301 AAATAAGTAA ATAAATCGAT AGTAGTGTAC TCCAAACGAG GCTGGAATAG CTTCTATTGT
            361 TGTTTCACAC TGGACTTCAA TTAAGTCTCA GTATTTTGCC ATACTCAATA TTAAGTACTA
            421 GGCTGGACGT GGTGGCTCAT GTCTGTAATC CCAGCACTTT GGGAGGCCGA GGTGGGTAGA
            481 TGGCTGGCTT GAGCTCAGGA GTTTGAAACC AGCCTGGGCA ACATGGTAAA ACCCCATCTG
            541 TACCCAAAAT ACAAAAATCA GCCAGGTGTG GTGGCACATG CCTGTGGTCC CAGGTACTTG
            601 GGAGGCTGAG GCAGGAGGAT GGCTTGAACC CAGGAGGTGG AGGCTGCAGT GAGCTATGAT
            661 GGCGCCACTG CACTCCAGCC TGGGTGACAG AGCGAGACCC TGTCTCAAAA ATCAAACAAA
            721 CAACCCCCTC GCCCCGGACA AAAGTAGTTT GCACTATTTT CTCATTTCAC AATATGTTTT
            781 TGAAATATTT CCCTTGAAAG GTAAGTCATA TTTATCATTC CTGTTGTATG GAGGCATCAT
            841 AAATTATTTC ACCATTCTAC CCTCCTTGAG TGTTGTGGCC TTTAGCCAGA CAAAACGCAG
            901 TGATGCCTAG AGCCAACTAG TGCCGTTTGT ATCTGTAGGT GTGCCTGCCA ACAGAAAATA
            961 TAAAGATACG ATCTGCACAA TTAGAAATCT ATACTAGACT AGAAATGCAG GGAAGAAAGT
           1021 TAGTCCGGGA TGAGGTGTAA CGACTTTCTA TACCCTCGAA ACTAAAACCA GACCAAATTG
           1081 AAACTACAAG GCACTCACTG GGCGTCCAAT GTCTGATACA TGAAGCCTGG CAACTCAGGC
          1141 TTAC
          //
          Primer: pGL4-136R
          Sequence file: RDB07686_A4Lmb.seq check
          >07686_07686_A4Lm_pGL4-136R_H07_22.ab1
              1 GCTTTCTAGT TTATGGCATC TTCATGGTGG CTTTACCAAC AGTACCGGAT TGCCAAGCTT
             61 GGCCGCCGAG GCCAGATCTT GATCCGTCTC CGCGACTACT TTATAGGCCA GACCTCCGCG
            121 CCTCGCCCAC TCTGGCCCCA AACCAATCGC CTCGCACGCG CTCAGCCCGC CCCCTGCGGC
            181 AGCCTCGGGT CGCGAGTGGC CGGGAATGAC TGGGGGCGGG GCCAAGGGGC CGCGTGCGCC
            241 GGAGACTCCC CCCGCCCCGC AGCCGGGAAA CGTCCAGAAC CCCGAAATGT GGAGAAAACA
            301 ACCTTCTTTT CACGGGGGCG TGGCCCGCCG GTTGCAGTAC GCGAAATTGG CAAAATGATG
            361 TAAACAGGGT ACGCTTTGAA AATCTGAAGT CACTTTGTCT AGAAATGTCA TTAGTTATGA
            421 CTGAGTTTGG CCACAGCGTC TCATTTGTTA CTCAGCAATT GGGACGCCCC AAGATGGATG
            481 CTTTTGTAGT TTTCATTTTT TTGTCTTGTT TTTTAGTTTT CTGAGGGTAT AGAAAGTCGC
            541 TACACCTCAA TCCCGGGACT TACCTTTTCT TCCCCTGCAA TTTTCTTAGT CCTTAGTATA
            601 GATTTTCTTA TTTGGTTGGC AGAATTCGGT ATTCTTTTTA TATTTTTCCT GTTTGGCAAG
            661 GCCACAACCC TACAGATAAC CAAACGGCAA CTAGTTGGCT TCTAGGCATC ACCTGCGTTT
            721 TTGTCTGGCC TAAAGGCCAC AACACTCAAG GAGGGTAGAA TGGTGAAATA ATTTATGATG
            781 CCTCCATACA ACAGGAATGA TAAATATGAC TTACCTTTCA AGGGAAATAT TTCAAAAACA
            841 TATTGTGAAA TGAGAAAATA GTGCAAACTA CTTTTGTCCG GGGCGAGGGG GTTGTTTGTT
            901 TGATTTTTGA GACAGGGTCT CGCTCTGTCA CCCAGGCTGG AGTGCAGTGG CGCCATCATA
            961 GCTCACTGCA GCCTCCACCT CCTGGGTTCA AGCCATCCTC CTGCCTCAGC CTCCCAAGTA
           1021 CCTGGGACCA CAGGCATGTG CCACCACACC TGGCTGATTT TGTATTTTGG GTACAGATGG
           1081 GGGTTTTACC ATGATGACCA GCTGCTTCAA CTCTTGAGCT CAAGGTCAGC ATCTACCAAC
          1141 TCGGACTTCC GAAATGCTGG TATACGTACA TTGAAGCGAT CTTAAACGT
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2023.11.09

          GNP_filter3_RDBDEP_html_231027.pl