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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3.1(-)-h Epidermal growth factor receptor

Catalog number RDB07600
Resource name pcDNA3.1(-)-h Epidermal growth factor receptor
Comment cDNA from BC094761.
Vector backbone pcDNA3.1(-) (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human epidermal growth factor receptor cDNA
Depositor|Developer Hamada, Hirofumi |
Other clones in our bank
External Database
Human epidermal growth factor receptor
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006). In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          提供案内 (日本国内) [open/close]

          提供条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ishii, K. et al., J. Gene Med. 10 (6): 597-609, 2008; Tanaka, T. et al., Clin. Cancer Res. 12 (12): 3803-3813, 2006)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB07600 pcDNA3.1(-)-h Epidermal growth factor receptor DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pcDNA3.1(-)-h Epidermal growth factor receptor was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07600).

          Reference section:

          Ishii, K., Nakamura, K., Kawaguchi, S., Li, R., Hirai, S., Sakuragi, N., Wada, T., Kato, K., Yamashita, T., Hamada, H., Selective gene transfer into neurons via Na,K-ATPase beta1. Targeting gene transfer with monoclonal antibody and adenovirus vector. J. Gene Med., 10, 597-609 (2008). PMID 18383483.
          Tanaka, T., Huang, J., Hirai, S., Kuroki, M., Kuroki, M., Watanabe, N., Tomihara, K., Kato, K., Hamada, H., arcinoembryonic antigen-targeted selective gene therapy for gastric cancer through FZ33 fiber-modified adenovirus vectors. Clin. Cancer Res., 12, 3803-3813 (2006). PMID 16778108.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07600_B1Fgp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,T7 pro,insert 5'
          Sequence file: RDB07600_B1Fga.seq check
          >07600_07600_B1Fg_3_CMV-Forward_E01_13_ABI24.ab1
              1 NNNNNNNNNN NNNNCNNTNN NNNCNNTGGG CGGTANGCGT GTACGGTGGG NGGTCTATAT
             61 AAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
            121 GACTCACTAT AGGGAGACCC AAGCTGGCTA GCGTTTAAAC GGGCCCTCTA GACTCGAGCG
            181 GCCGCCGCCG CCCAGACCGG ACGACAGGCC ACCTCGTCGG CGTCCGCCCG AGTCCCCGCC
            241 TCGCCGCCAA CGCCACAACC ACCGCGCACG GCCCCCTGAC TCCGTCCAGT ATTGATCGGG
            301 AGAGCCGGAG CGAGCTCTTC GGGGAGCAGC GATGCGACCC TCCGGGACGG CCGGGGCAGC
            361 GCTCCTGGCG CTGCTGGCTG CGCTCTGCCC GGCGAGTCGG GCTCTGGAGG AAAAGAAAGT
            421 TTGCCAAGGC ACGAGTAACA AGCTCACGCA GTTGGGCACT TTTGAAGATC ATTTTCTCAG
            481 CCTCCAGAGG ATGTTCAATA ACTGTGAGGT GGTCCTTGGG AATTTGGAAA TTACCTATGT
            541 GCAGAGGAAN TATGATCTTT CCTTCTTAAA GACCATCCNG GANGTGGCTG GNTATGTCCT
            601 CNTTGCCCTC NACACAGTGG AGCGAATTCC NTTGGAAAAC CTGCAGATCA TCAGAGGAAA
            661 TNNGTACTAC NAAANNNNTA TGNCNTANCN NTCNNATCNN NNTATGANGC NNNAAAANNN
          721 GANNGNANNN NNNNNNN
          //
          Primer: bGH_rev3 (Pr0885)
          Region: bGHp A,insert 3'
          Sequence file: RDB07600_B1Fgb.seq check
          >07600_07600_B1Fg_3_bGB_rev3_F01_16_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNGGGCNN ACAACAGATG GCTGGCAACT AGAAGGCACA
             61 GTCGAGGCTG ATCAGCGGTT TAAACTTAAG CTTGGTACCG GGCCCCCCCT CGAAGGATCA
            121 GGCCCTTATG GCCGGATCCA ACGGTGGAGA GTTTTTTTTT TTTTTTTCTG GATAAAGCTT
            181 AAAATTTATT AATATTAACT AATATTTAAT TAGTTAAATA TACGTTTCAC AGGTCAAGAT
            241 AGAGCCAGCA GAGAGAACAG GGATCAAATC TGTCACAAAC ACACACATAC ACACACACAC
            301 ATACACAGAT GTACACACAT CATGAACACT CACACATACA GATGTGTATG TCCGTGATTT
            361 GCTTCATAAG CTAGAAATTT TGCAGATTCA TTCTTACAAC AGAGGTACAG CAAACAACCA
            421 GACACAACAA AGAGCTTGTG CAGGCTCGGT CATGTGTTTA TCTGCTCCTT ACGCCCTTCA
            481 CTGTGTCTGC AAATCTGCCA CTGTTTCCCC CTCTGGAGAT GCTGGAGGGA GCANCTGGCT
            541 TGGACACTGG AGACTGGCTG CTGCTGAGTG GGTCNAGGAN GTTGANGANC AGGANTGTTT
            601 CCAGACNAAG CCACTCACCA NNCACTGNNN NNAANGTGTC NTCTATGCTG TCCTCNGTNN
          661 NNGCNNCTGN NNGNNCNNAG CNGNATCNNT NCNNNAANNN NTGTNNNNNN NNNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB07600_B1Fgc.seq check
          >07600_07600_B1Fg_3_SV40pro_ori_F_G01_19_ABI24.ab1
              1 NNNNNNNNNN NNNNNNCGCC TCNGCCTCTG NNCTATTCCN GAAGTAGTGA GGAGGCTTTT
             61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG
            121 ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG CACGCAGGTT
            181 CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG ACAATCGGCT
            241 GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT TTTGTCAAGA
            301 CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA TCGTGGCTGG
            361 CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG GGAAGGGACT
            421 GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT GCTCCTGCCG
            481 AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT CCGGCTACCT
            541 GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG ATGGAAGCCG
            601 GTCTTGTCGA TCAGGANGAT CTGGACGAAN AGCATCANGG GCTCGCGCNN GCCGAACTGT
            661 NNGCNNNGCT NNANNGNGCG NATGNNNNAN GGCNANGNAN CTNNNTCNNG ACCNANGGNN
          721 NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Ishii, K., Selective gene transfer into neurons via Na,K-ATPase beta1. Targeting gene transfer with monoclonal antibody and adenovirus vector. J. Gene Med., 10, 597-609 (2008). PMID 18383483.
          original Tanaka, T., arcinoembryonic antigen-targeted selective gene therapy for gastric cancer through FZ33 fiber-modified adenovirus vectors. Clin. Cancer Res., 12, 3803-3813 (2006). PMID 16778108.

          2023.05.01

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