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pGL4-phNQO1

Promoter collection, Human NQO1 promoter

Catalog number RDB07570
Resource name pGL4-phNQO1
Alternative name U2796(2174)-3
Clone info. PCR amplified human NQO1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 69727985 to 69726587(NC_000016.10); relatively -1355 to +44, where +1 corresponds to 1 nt of NM_000903.2.
Comment PCR cloning, forward primer: ATTTTGTCAGATGGGGTCTCAC; reverse primer: ATATCCTGTCCGGCCCGTTT.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human NAD(P)H dehydrogenase, quinone 1 genomic DNA
Depositor|Developer DNA Bank, | Kishikawa, Shotaro |
Other clones in our bank
External Database
Human NAD(P)H dehydrogenase, quinone 1
          Reference sequence
            
           
          Remarks, protocol and/or map (pdf) RDB07570.pdf

          Distribution information

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          Additional terms and conditions:
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB07570 pGL4-phNQO1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pGL4-phNQO1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07570).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          Tips

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07570_A7Etp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F (Pr0081)
          Region: insert 5'
          Sequence file: RDB07570_A7Eta.seq check
          >07570_07570_A7Et_pGL4-4174F_C02_03_ABI08.ab1
              1 GCCTGGGGTC CGGACATTTT CTCTGGCCTA CTGGCCGGTA CCTGAGCTCG CTAGCCTCGA
             61 GGATATTTTG TCAGATGGGG TCTCACTTTG TTGCTCAGGC TTTTCTCAAA CTCTTGGGCT
            121 CAAGTGATCC TCCTGCCTCA GCCTCCCAAA GTAATTTGTA AATACGTGTA AATAATATAG
            181 TCCTCTCTTG TTGCATCAAA ATGAATGCAA AAATGTCACG ATGGGGCCGG GCGCGGTGGC
            241 TCATGCCTGT AATCCCAGCA CTTCGGGAGG CCGAGGCGGT AGGATCACCT GAGGTCGGGA
            301 GTTGGAAACC AGTTTTCTGG TGCCTCCTTA ACTTTTGCAC TGGAGGGACA ACGGAAGGGC
            361 CCTGTGTCAT CCTCACCTCC CCCATCTGTG ATTCTTCTGA TGGTTAATTA CCACTTTAAA
            421 GCTCTAGTTC TTTTTCCTTC ACCTACTGAG AGTATATCTC CTTCTTTACA GTAACTTTTA
            481 CTTTATAGAG TTTTTTTTGA GACGGAGTCT CACTTCGTCA CCCAGGCGGG AGTGCAGTGG
            541 CACGATCTCG ACCCACTGCA ACCTCCGCCT TCCGGGTTCA AGCGATTCTC CTGCCTCAGC
            601 CTCCCGAGTA GCTGGGACTT ACAGGCGCCC GCCATCACGC CCAGCTAATT TTTCAGTACA
            661 GACGGGGCTT CACCAGGTTG GCCAGGCTGG TCTCGAACTC CTGACCTCAG GTGATCCACC
            721 CACCTCGGGC CTCCCATATT GCTGGGATTA CAGGCGTGAG CCACCGCGCC TGGGTCCCGT
            781 TTTGGCTATT CTTTAATATG CTTCCCCTAT AACTGCTATC TCCACACCCA GCCTTGCCTT
            841 AGCTCATGGG AAACAAAACC AAATTAGTTC CCCCCATTAC CCTGCCTTGA AGGAGCAGGG
            901 GTGGGTGCAG TGGCATGGCC CTCCCGGGGG AAGTGGTGTT TGTATGGGCC CCCCCAGGTT
          961 GAAGAATCCT GGAAT
          //
          Primer: pGL4-136R (Pr0079)
          Region: Luc, insert 3'
          Sequence file: RDB07570_A7Etb.seq check
          >07570_07570_A7Et_pGL4-136R_D02_04_ABI08.ab1
              1 GCCCCTATGG TTTTTGGCAT CTTCCATGGT GGCTTTACCA ACAGTACCGG ATTGCCAAGC
             61 TTGGCCGCCG AGGCCAGATC TTGATATATC CTGTCCGGCC CGTTTGAGGT GGACAGCCTA
            121 CAAGGGCGGG ACCCAACGCC TGAATCCTGG GGTGCTGGGC GGAGGATGCT GAGGCTGCAG
            181 CTCCAGGAAG GGAGGGAGCC TGGGGGATGA AGGAGGGGCC TCTCCACTCT CACCCGCCGG
            241 AGCAACTGGT GGAAGTCAAG AAAAGTCTCT CGGGGCTGGG GGAGGGGATT GTAGGGGTGT
            301 GTGTGACAGA GGCCTCAAAA ATCTGGCTGG GGGTGCTAAG GCAAGCCCAT CTGCGCACTT
          361 TGCCCTGAGG TGCAAGAGG
          //
          Primer: RDB07570#1 (Pr0467)
          Region: insert mid
          Sequence file: RDB07570_A7Etc.seq check
          >07570_07570_A7Et_RDB07570#1_H10_08_ABI08.ab1
              1 AAGTCGCTGT TCGACTCCTG ACCTCAGGTG ATCCACCCAC CTCGGCCTCC CATATTGCTG
             61 GGATTACAGG CGTGAGCCAC CGCGCCTGGT CCCGTTTTGG CTATTCTTTA ATATGCTTCC
            121 CTATAACTGC TATCTCCACA CCAGCCTTGC CTTAGCTCAT GGGAAACAAA ACCAATTAGT
            181 TCCCCCATTA CCTGCCTTGA GGAGCAGGGG TGGTGCAGTG GCATGCACCC AGGGAAGTGT
            241 GTTGTATGGG CCCCCAAGTG CAGAATCTGA ATCTTGCAAA GGAAGAAACA AATTCGTCTC
            301 CACGGAGCAT GTCTCCCCAG GACTCTCAGC CTTCCAAATC CGCAGTCACA GTGACTCAGC
            361 AGAATCTGAG CCTAGGGCAC CACAGTAATC CGCATCCGGC TCTTTTTCTG CTCCATTTCG
            421 TGCCAAGGCT AAAAGGGCAT GCCCACTTGA TCCCTGGACT CTCTTGGGAC GACTTCCACC
            481 CTGCATCCTC TTGCACCTCA GGGCACAGTG CGCAGATGGG CTTGCCTTAG CACCCCCAGC
            541 CAGATTTTTG AGGCCTCTGT CACACACACC CCTACAATCC CCTCCCCCAG CCCCGAGAGA
            601 CTTTTCTTGA CTTCCACCAG TTGCTCCGGC GGGTGAGAGT GGAGAGGCCC CTCCTTCATC
            661 CCCCAGGCTC CCTCCCTTCC TGGAGCTGCA GCCTCAGCAT CCTCCGCCCA GCACCCCAGG
            721 ATTCAGGCGT TGGGTCCCGC CCTTGTAGGC TGTCCACCTC AAACGGGGCC GGACAGGGAT
            781 ATATCAAGAT CTGGCCTCGG CGGCCAAGCT TGGCAATCCG GTACTGTTGG GTAAAGCCAC
            841 CATGGAAGAT GCCAAAAAAC ATTAAGAAAG GGGCCCAGCG CCATTCTACC CACTCGAAAG
            901 ACGGGAACCG CCCGGCGAGC AGCTGCCACA AAAGGCCCAT GAAAGCGCCT ACGCCCCTTG
            961 GATGCCCCGG GCACCCAATT CGCCCTTTTT AACCGACGGC CACATATCGA AGTGACATTT
           1021 ACCTTACCGT CCGAGTACTT TCGAGAATGA GCGATCCGGC TTGGCAGAAG CTATATGGAA
          1081 AGGCCGCCT
          //
          Primer: pAxCALNLF1 (Pr0166)
          Region: insert 3'
          Sequence file: RDB07570_A7Etd.seq check
          >07570_07570_A7Et_pAxCALNL_F1_E02_05_ABI08.ab1
              1 ACCAAGGGTA TCTTATCATG TCTGCTCGAA GCGGCCGGCC GCCCCGACTC TAGAATTATT
             61 ACACGGCGAT CTTGCCGCCC TTCTTGGCCT TAATGAGAAT CTCGCGGATC TTGCGGGCGT
            121 CCAACTTGCC GGTCAGTCCT TTAGGCACCT CGTCCACGAA CACAACACCA CCGCGCAGCT
            181 TCTTGGCGGT TGTAACCTGG CTGGCCACAT AGTCCACGAT CTCCTTCTCG GTCATGGTTT
            241 TACCGTGTTC CAGCACGACG ACTGCGGCGG GCAGCTCGCC GGCATCGTCG TCGGGCAGGC
            301 CGGCGACCCC GGCGTCGAAG ATGTTGGGGT GTTGCAGCAG GATGCTCTCC AGTTCGGCTG
            361 GGGCTACCTG GTAGCCCTTG TATTTGATCA GGCTCTTCAG CCGGTCCACG ATGAAGAAGT
            421 GCTCGTCCTC GTCCCAGTAG GCGATGTCGC CGCTGTGCAG CCAGCCGTCC TTGTCGATGA
            481 GAGCGTTTGT AGCCTCGGGG TTGTTAACGT AGCCGCTCAT GATCATGGGG CCACGGACGC
            541 ACAGCTCGCC GCGCTGGTTC ACACCCAGTG TCTTACCGGT GTCCAAGTCC ACCACCTTAG
            601 CCTCGAAGAA GGGCACCACC TTGCCTACTG CGCCAGGCTT GTCGTCCCCT TCGGGGGTGA
            661 TCAGAATGGC GCTGGTTGTT TCTGTCAGGC CGTAGCCCTG GCGGATGCCT GGTAGGTGGA
            721 AGCGTTTGGC CACGGCCTCA CCTACCTCCT TGCTGAGCGG CGCCCCGCCG CTGGCGATCT
            781 CGTGCAAGTT GCTTAGGTCG TACTTGTCGA TGAGAGTGCT CTTAGCGAAG AAGCTAAATA
            841 GTGTGGGCAC CAGCAGGCAG ATTGAATCTT ATAGTCTTGC AAGCTGCGCA AGAATAGCTC
            901 CTCCTTCGAA GCGGTACATG AGCACGACCC GAAAGCCGCA GATCAAGTAG GCCCAGCGTG
            961 GATGAACATG CCGAAAGCCC GTGGTGAATG GGCACACGCT GAGGATAGCG TGTCGGGGAT
           1021 GATCCTGGAT GGCGAGATTG GGGGTCGCGG GGCATGACTG ATTCGGACAC AGCGTTGCCG
          1081 GTGCCGATG
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2023.11.09

          GNP_filter3_RDBDEP_html_231027.pl