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pGL4-phMMP9

Promoter collection, Human MMP9 promoter

Catalog number RDB07537
Resource name pGL4-phMMP9
Alternative name U2792-2
Clone info. PCR amplified human MMP9 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 46007674 to 46009018(NC_000020.11); relatively -1234 to +111, where +1 corresponds to 1 nt of NM_004994.3.
Comment PCR cloning, forward primer: AGGGCTGGAGAACTGAAAGG; reverse primer: AAGAGCACAAGGGTGGACTG. Known difference : Deletion at 46,008,773 to 46,008,778. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase) genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
          Reference sequence
            
           
          Remarks, protocol and/or map (pdf) RDB07537.pdf

          Distribution information

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          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB07537 pGL4-phMMP9 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pGL4-phMMP9 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07537).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

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          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14426_A6Kgp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-4174F
          Sequence file: RDB14426_A6Kga.seq check
          >07537_A6Kg_4_pGL4-4174F_D11_11_ABI24.ab1
              1 ACTCAGGGGG CAAGAACATT TCTCTGGCCT AACTGGCCGG TACCTGAGCT CGCTAGCCTC
             61 GAGGATAGGG CTGGAGAACT GAAAGGGCTC CTATAGATTA TTTTCCCCCA TATCCTGCCC
            121 CAATTTGCAG TTGAAGAATC CTAAGCTGAC AAAGGGGAAG GCATTTACTC CAGGTTACAC
            181 TGCAGCTTAG AGCCCAATAA CCTGGTTTGG TGATTCCAAG TTAGAATCAT GGTCTTTTGG
            241 CAGGGTCTCG CTCTGTTGCC CAGGCTGGAG TGCAGTGACA TAATCATGGC TCACTGTATC
            301 CTTGACCTTC TTTCTGGGCT CAAGCAATCC TCCCACCTCG GCCTCCCAAA GTGCTAAGAT
            361 TACAGGAATG AGCCACCATA CCTGGCCCTG AATCTTGGGT CTTGGCCTTA GTAATTAAAA
            421 CCAATCACCA CCATCCGTTG CGGACTTACA ACCTACAGTG TTCTAAACAT TTTATATGTT
            481 TGATCTCATT TAATCCTCAC ATCAATTTAG GGACAAAGAG CCCCCCACCC CCCGTTTTTT
            541 TTTTTACAGC TGAGGAAACA CTTCAAAGTG GTAAGACATT TGCCCGAGGT CCTGAAGGAA
            601 GAGAGTAAAG CCATGTCTGC TGTTTTCTAG AGGCTGCTAC TGTCCCCTTT ACTGCCCTGA
            661 AGATTCAGCC TGCGGAAGAC AGGGGGTTGC CCCAGTGGAA TTCCCCAGCC TTGCCTAGCA
            721 GAGCCCATTC CTTCCGCCCC CAGATGAAGC AGGGAGAGGA AGCTGAGTCA AAGAAGGCTG
          781 TCAGGGAGGG AAAAAGAGGA CAGAGCCTGG AGTGTGGGGA GGGGGTTTGG GGGAGG
          //
          Primer: pGL4-136R
          Sequence file: RDB14426_A6Kgb.seq check
          >07537_A6Kg_4_pGL4-136R_E11_14_ABI24.ab1
              1 GGTTTAGTTT TGGCATCTTC CATGGTGGCT TTACCAACAG TACCGGATTG CCAAGCTTGG
             61 CCGCCGAGGC CAGATCTTGA TAAGAGCACA AGGGTGGACT GGCGCTGTCT GGGGGCAGCA
            121 AAGCAGCAGC CCAGCACCAG GAGCACCAGG ACCAGGGGCT GCCAGAGGCT CATGGTGAGG
            181 GCAGAGGTGT CTGACTGCAG CTGCTGTTGT GGGGGCTTTA AGGAGGCGCT CCTGTGACCC
            241 CACCCCTCCT TGACAGGCAA GTGCTGACTC AGGGGTCAGG GTGTGTGTGT GTGTGTGTGT
            301 GTGTGTGTGT GTGTGTGTGT GGCAGCACCA GCATGAGAAA GGGCTTACAC CACCTCCTCC
            361 TCTCTCCACT GCCCGCAGTC TCCACTGCCA AGTCAGGCAA GACCCCAGAC CCGCAGGAAA
            421 CCGCAGGCCC TCAGGGGAGG GGTAGGGTTT TGCAAACTGC AGAGCTTGTG GGAACTGTAT
          481 GAAAGGGAGG GAGGGGGATG A
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB14426_A6Kgc.seq check
          >07537_A6Kg_4_pAxCALNL_F1_F11_17_ABI24.ab1
              1 ATCAATGTAT CTATCATGTC TGCTCGAGCG GCCGGCCGCC CCGACTCTAG AATTATTACA
             61 CGGCGATCTT GCCGCCCTTC TTGGCCTTAA TGAGAATCTC GCGGATCTTG CGGGCGTCCA
            121 ACTTGCCGGT CAGTCCTTTA GGCACCTCGT CCACGAACAC AACACCACCG CGCAGCTTCT
            181 TGGCGGTTGT AACCTGGCTG GCCACATAGT CCACGATCTC CTTCTCGGTC ATGGTTTTAC
            241 CGTGTTCCAG CACGACGACT GCGGCGGGCA GCTCGCCGGC ATCGTCGTCG GGCAGGCCGG
            301 CGACCCCGGC GTCGAAGATG TTGGGGTGTT GCAGCAGGAT GCTCTCCAGT TCGGCTGGGG
            361 CTACCTGGTA GCCCTTGTAT TTGATCAGGC TCTTCAGCCG GTCCACGATG AAGAAGTGCT
            421 CGTCCTCGTC CCAGTAGGCG ATGTCGCCGC TGTGCAGCCA GCCGTCCTTG TCGATGAGAG
            481 CGTTTGTAGC CTCGGGGTTG TTAACGTAGC CGCTCATGAT CATGGGGCCA CGGACGCACA
            541 GCTCGCCGCG CTGGTTCACA CCCAGTGTCT TACCGGTGTC CAAGTCCACC ACCTTAGCCT
            601 CGAAGAAGGG CACCACCTTG CCTACTGCGC CAGGCTTGTC GTCCCCTTCG GGGGTGATCA
            661 GAATGGCGCT GGTTGTTTCT GTCAGGCCGT AGCCCTGGCG GATGCCTGGT AGGTGGAAGC
            721 GTTTGGCCAC GGCCTCACCT ACCTCCTTGC TGAGCGGCGC CCCGCCGCTG GCGATCTCGT
            781 GCAAGTTGCT TAGGTCGTAC TTGTCGATGA GAGTGCTCTT AGCGAAGAAG CTAAATAGTG
            841 TGGGCACCAG CAGGGCAGAT TGAATCTTAT AGTCTTGCAA GCTGCGCAAG AATAGCTCCT
            901 CCTCGAAGCG GTACATGAGC ACGACCCGAA AGCCGCAGAT CAAGTAGCCC AGCGTGGTGA
            961 ACATGCCGGA AGCCGTGGTG AAATGGCACC ACGCTGAGGG ATAGCGGTGT CGGGGATGAT
           1021 CTGGGTTGCC GAAGATTGGG GTCGCGGGCA TGACTGAATC GGGACACAGC GGTGCGGTGC
           1081 GGTAGGGGCT TACGGCCATG GCCATCCCAG GCAACTGCCA CTACCTGGTT TTCAATGGAA
          1141 TTCAGGGGCT CGCGA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2023.11.09

          GNP_filter3_RDBDEP_html_231027.pl