RDB07404 - pGL4-phRHOA

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pGL4-phRHOA

Promoter collection, Human RHOA promoter

Catalog number	RDB07404
Resource name	pGL4-phRHOA
Alternative name	U2765(2143)-10
Clone info.	PCR amplified human RHOA promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 49413224 to 49411909(NC_000003.12); relatively -1127 to +189, where +1 corresponds to 1 nt of NM_001664.4.
Comment	PCR cloning, forward primer: CAAAGGCCATTAAGCTGGTG; reverse primer: ACCAGACCGTGGACTAACGA. Entire sequence of promoter region has not been confirmed.
Vector backbone	pGL4.10 [Luc2] (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human ras homolog gene family, member A genomic DNA
Depositor\|Developer	DNA Bank, \|

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human ras homolog gene family, member A

External Database
Human ras homolog gene family, member A

Reference sequence

Distribution information

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions for distribution	The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
Ordering	Please visit Information of Request for Distribution.[link] Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB07404	pGL4-phRHOA	DNA solution

Please review the QC test results indicated by check

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References and tips

Electronic file

Remarks, protocol and/or map (pdf)	RDB07404.pdf

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content	Promoter Collection (Firefly Luciferase) (English text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB07404_A4D2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Sequence file: RDB07404_A4D2a.seq
>07404_07404_A4D2_1_pGL4-4174F_A12_03.ab1 1 TAAGGGGGTC CGGACATTTT CTCTGGCCTA ACTGGCCGGT ACCTGAGCCC GCTAGCCTCG 61 AGGATCAAAG GCCATTACGC TGGTGGGCTT AAAGCCTGAG AACTCCCCTC TCTGTCACCA 121 GCACAGGAGT GTCCTTGGAA TGCCAAGCAT GGGGTTGCGG TATGGACAGA TTTACTCACC 181 ACGAAGGGAA ATGCGTGGAG CCATCAGGCG TGGATCCATT CTGACCACCC AGACCTGGAG 241 GCAGAAACAC ATCCAGAGCT GCAGAAGGCT GGGATGGGAA TCTGAGCAGG CATCAGCCCA 301 GGTCAGGTTA GAGACCACTG GTATTGTTTG GTGAAGTCTG GTGAGGGTCC TAAGGGACTA 361 ATGGTATTGC CAGGTGAGAG TTCTGAGGAA CAGATACAAT GTGATGGGTG GACTGGTGGG 421 GATTCTGGCG TAAAATGGTA TGGTGGCATG AGTCAGGGCA CAACGTTAAG TTCTCCCATC 481 CAAGTACCTG GCTTAGCTTC CGAGGTCAGA TGCGTTCAGG GTAGTAAGGC CGTGAACCAA 541 TGTTAAGTTC TGATGAGCCT CTTGCAGGAT AGTGGGGAAC CCAAATGGTA CAGTCGGGAG 601 AGGGTGGGGG CGGGGGCGGG CCAGCCCACG GAGGTTCGTA GAAGCCTCAC CTGGACGGTG 661 ATACAACCCG GTCACTGTAT TCCCGTAGAA CCCCCACGCC AGCTGGATGC CCAGGAGACT 721 TAACACCACC ACAGCAGCAG CAGCTTGAGA GGTCACGCGC ATGTCCTGCG CCTCCCAACT 781 CCCGCAAGAA CTCGCTGCCC AGCGCGCCCA GCACCGTGGC GCAAGAGCTG CAGCTTGCCC 841 AGACAGACTC CGCCATGAAC TACCCTCGCC CACCGGGTAC TGTCACCGTA TCCACCACGC 901 ACGCGTGTGT GCTTCGGGAA CAGGTCGTAT GGCCCACGAT ACCAGCCAGG TGGGCCTAAC 961 TTCCGTGCGT GAAAGATTGG CATTCCGGGG AGAGATATAA ACGCAAACGC CATACCACAC 1021 CTGCTGCTTA GGGATAGTAT TCGGGATAAA TAGCAG //
Primer: Sequence file: RDB07404_A4D2b.seq
>07404_07404_A4D2_1_pGL4-136R_D12_12.ab1 1 GGGCCCTTGT TTTTTTGGGC ATCTTCCATG GTGGCTTTAC CAACAGTACC GGATTGCCAA 61 GCTTGGCCGC CGAGGCCAGA TCTTGATACC AGACCGTGGA CTAACGAGAG AACCGACGGA 121 GGACCGCGGG CGGCGGGAGG GTAGCGCGAG AGAGCGAGGG CGGGCGGCGG CGGGAGGGCG 181 GGGAACGACG TGCGCGCCTC CGAGTGCCCA GCGGGCTCGA GCCGGCACAG GTCGCGGCCC 241 CGCGCACGCG CGCGCACTCA CAGCTCATCC ACTATTGCTC AGGAGCCCGT CCACGCCCTA 301 AAAGCAAAAC CCTTAACCAA TCATTGCAGC CGCGTGGGGC ATCACGGGAA CTCCGGGGCT 361 ATAAAAGACC TGGTTGCTAT TTTATACTCC CGGAGTCACT CATCCCTTAA GCAAGCAGGG 421 TGGGGTTAGG TGCGCGTGCG CGGTTTTAAT ACTCCTCCCC GAACTGCCAA CTCTTCACGC 481 ACGCGAAGTA GGCCCCACCC TGGCTGGGTT TACGCGTGCG CACTAACGGG CCTGGTCCCG 541 GAAGACCACA CGCGTGCGTG GTGGGGACTA CGGTGACAGT ACCCCGGGTG GGGCGAGGGC 601 CAGTCATGGC GGAGTCCTGG TCTGGGCAGG CCTTGCAGGC TCTGCCGGCC ACGGTGCTGG 661 GCGCGCTGGG CAGCGAGTTC TTGCGGGAGT GGGAGGCGCA GGACATGCGC GTGACCCTCT 721 TCAAGCTGCT GCTGCTGTGG TTGGTGTTAA GTCTCCTGGG CATCCAGCTG GCGTGGGGGT 781 TCTACGGGAA TACAGTGACC GGGTTGTATC ACCGTCCAGG TGAGGCTTCC TACGAACCTC 841 CGTGGGCTGG CCGCCCCCGC CCCCACCCTC TCCCGACTGT ACCATTGGGT TCCCCACTAT 901 CCTGCAAGAG GCTCATCAGA ACTTAACATT GGGTTCACGG CCTTACTACC CTGAACGCAT 961 CTGACCTCGG AAGCTAAGCC AGGTACTTGG ATGGGAGAAC TTAACGTTGT GCCCTGACTC 1021 ATGCCCACCA TACCATTTTT ACGCCCAGAA TCCCCACCAA GTCCCACCCC ATCACATTTG 1081 TATCTGTTCC TCAAGAACTC TCAACTGCAT TACAATTTAA GTTCCTTAAG GGAACCCCTC 1141 CACCCAGAAA CTTTCCCACC CAAGGA //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl