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pGL4-phPLK1

Promoter collection, Human PLK1 promoter

Catalog number RDB07327
Resource name pGL4-phPLK1
Alternative name U2688(2066)-2
Clone info. PCR amplified human PLK1 promoter sequence was inserted into a firefly luciferase vector. Cloned fragment: 23677730 to 23678950(NC_000016.10); relatively -1042 to +179, where +1 corresponds to 1 nt of NM_005030.6.
Comment PCR cloning, forward primer: AGGGTTGCCACTCAAGTACC; reverse primer: AGTCACTGCAGCACTCATGC. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human polo-like kinase 1 genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank

External Database
Human polo-like kinase 1

          Reference sequence
            
           
          Sequence (full) RDB07327hts01.seqcheck assembled from experimentally sequenced data
          Remarks, protocol and/or map (pdf) RDB07327.pdf
          Test sheet RDB20129_B3K9p1-1.pdf 

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions:
          The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB07327 pGL4-phPLK1 DNA solution


          How to cite this biological resource

          Materials & Methods section:

          The pGL4-phPLK1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07327).

          Reference section:

          Further references such as user reports and related articles (go to bottom)


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          Featured content Promoter Collection (Firefly Luciferase) (English text)

          References

          Original, user report and related articles


          2024.03.26

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