Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGL4-phBRCA1

Promoter collection, Human BRCA1 promoter

Catalog number RDB07296
Resource name pGL4-phBRCA1
Alternative name U2642 (2020)-1
Clone info. PCR amplified human BRCA1 promoter sequence was inserted into a firefly luciferase vector. Genomic DNA (43,125,397 - 43,126,526nt of NC_000017.11) corresponding to 1 to 87nt of NM_007294.3 and 1.0 kb up-stream was cloned.
Comment PCR cloning, forward primer: tcctcccttgaacttctcca; reverse primer: atctgtaattcccgcgcttt. Entire sequence of promoter region has not been confirmed.
Vector backbone pGL4.10 [Luc2] (Plasmid)
Selectable markers Amp^r
Gene/insert name Human breast cancer 1, early onset genomic DNA
Depositor|Developer DNA Bank, | Pan, Jianzhi |
Other clones in our bank
External Database
Human breast cancer 1, early onset
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form           [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 謝辞の表明を必要とする。
          付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB07296 pGL4-phBRCA1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB07296.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Firefly Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07296_A7I6p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pGL4-136R
          Region: Luc,insert3'
          Sequence file: RDB07296_A7I6b.seq check
          >07296_07296_A7I6_pGL4-136R_B01_02_ABI08.ab1
              1 GCCTAAAGTT TTGGCATCTT CCATGGTGGC TTTACCAACA GTACCGGATT GCCAAGCTTG
             61 GCCGCCGAGG CCAGATCTTG AATCTGTAAT TCCCGCGCTT TTCCGTTGCC ACGGAAACCA
            121 AGGGGCTACC GCTAAGCAGC AGCCTCTCAG AATACGAAAT CAAGGTACAA TCAGAGGATG
            181 GGAGGGACAG AAAGAGCCAA GCGTCTCTCG GGGCTCTGGA TTGGCCACCC AGTCTGCCCC
            241 CGGATGACGT AAAAGGAAAG AGACGGAAGA GGAAGAATTC TACCTGAGTT TGCCATAAAG
            301 TGCCTGCCCT CTAGCCTCTA CTCTTCCAGT TGCGGCTTAT TGCATCACAG TAATTGCTGT
            361 ACGAAGGTCA GAATCGCTAC CTATTGTCCA AAGCAGTCGT AAGAAGAGGT CCCAATCCCC
            421 CACTCTTTCC GCCCTAATGG AGGTCTCCAG TTTCGGTAAA TATAAGTAAT AAGGATTGTT
            481 GGGGGGGTGG AGGGAAATAA TTATTTCCAG CATGCGTTGC GGAATGAAAG GTCTTCGCCA
            541 CAGTGTTCCT TAGAAACTGT AGTCTTATGG AGAGGAACAT CCAATACCAG AGCGGGCACA
            601 ATTCTCACGG AAATCCAGTG GATAGATTGG AGACCTGTGC GCGCTTGTAC TTGTCAACAG
            661 TTATGGACTG GAGTGTTATG TTTTCGTATT TTGAAAGCAG AAACTAGGCC TTAAAAAGAT
            721 ACGTACAACT CTTTAGGGAG ACTACAATTC CCATCCAGCC CCAGGAGTCT GGGGCAAGTA
            781 GTCTTGTAAG GTCAGTGGCC TGCGGGGACG CAGTGAGCGC CGAATTTGCC TGGGGCAGGG
            841 GAAATGCGCT CTGGCCCATG TCTGCGCACT CGTAGTTCCA CCCCCTCAGC CCCAGTGTTT
            901 GTTATTTTTC GGGTTCAGCT TGCTTTTTGC CCCGTCTCCG TCGACGCAAT CGCCACCAGT
            961 CAATGGGGGT GGGTCGTTTT TGAGGGACAA GTGGTAAGAG CCAAATCTTT CTTGGCGAAA
           1021 AACGCGGAGG AAACGGGACT AGTTTACTGT CTTTGTCCCG CCCATGTTAG ATTCCACCCC
          1081 CCCACAGAAG ATAGCCGCCA GAGCTGGCAG GCGGAACGGA TTCCTTTTTG CCAT
          //
          Primer: pAxCALNLF1
          Region: Sv40a(A),Luc
          Sequence file: RDB07296_A7I6c.seq check
          >07296_07296_A7I6_pAxCALNL_F1_C01_03_ABI08.ab1
              1 ATAGGGATAT CTTTATCATG TCTGCTCGAA GCGGCCGGCC GCCCCGACTC TAGAATTATT
             61 ACACGGCGAT CTTGCCGCCC TTCTTGGCCT TAATGAGAAT CTCGCGGATC TTGCGGGCGT
            121 CCAACTTGCC GGTCAGTCCT TTAGGCACCT CGTCCACGAA CACAACACCA CCGCGCAGCT
            181 TCTTGGCGGT TGTAACCTGG CTGGCCACAT AGTCCACGAT CTCCTTCTCG GTCATGGTTT
            241 TACCGTGTTC CAGCACGACG ACTGCGGCGG GCAGCTCGCC GGCATCGTCG TCGGGCAGGC
            301 CGGCGACCCC GGCGTCGAAG ATGTTGGGGT GTTGCAGCAG GATGCTCTCC AGTTCGGCTG
            361 GGGCTACCTG GTAGCCCTTG TATTTGATCA GGCTCTTCAG CCGGTCCACG ATGAAGAAGT
            421 GCTCGTCCTC GTCCCAGTAG GCGATGTCGC CGCTGTGCAG CCAGCCGTCC TTGTCGATGA
            481 GAGCGTTTGT AGCCTCGGGG TTGTTAACGT AGCCGCTCAT GATCATGGGG CCACGGACGC
            541 ACAGCTCGCC GCGCTGGTTC ACACCCAGTG TCTTACCGGT GTCCAAGTCC ACCACCTTAG
            601 CCTCGAAGAA GGGCACCACC TTGCCTACTG CGCCAGGCTT GTCGTCCCCT TCGGGGGTGA
            661 TCAGAATGGC GCTGGTTGTT TCTGTCAGGC CGTAGCCCTG GCGGATGCCT GGTAGGTGGA
            721 AGCGTTTGGC CACGGCCTCA CCTACCTCCT TGCTGAGCGG CGCCCCGCCG CTGGCGATCT
            781 CGTGCAAGTT GCTTAGGTCG TACTTGTCGA TGAGAGTGCT CTTAGCGAAG AAGCTAAATA
            841 GTGTGGGCAC CAGCAGGGCA GATTGAATCT TATAGTCTTG CAAGCTGCGC AAGAATAGCT
            901 CCTCCTCGAA GCGGTACATG AGCACGACCC GAAAGCCGCA GATCAAGTAG CCCAGCGTGG
            961 TGAAACATGC CGAAGCCGTG GTGAAATGGC ACCCACGGCT GAGGATAGCC GGTGTCGGGG
           1021 GATGATCTGG GTTTGCCCGA AGATGGGGGT CGCGGGCATG ACTGAATTCG GACACAGCGT
          1081 TGCGGATGCG GTTTAGGACT ACGCCCCTTT GGGGCCA
          //
          Primer:
          Sequence file: RDB07296_A7I6d.seq check
          1

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl