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pCMFlag_hsSMAD3

Expression vector of human SMAD3.

Catalog number RDB07019
Resource name pCMFlag_hsSMAD3
Alternative name SET-0137_3-8-1
Clone info. Expression vector of human SMAD3. Expression has not yet been confirmed.
Comment PCR cloning, forward primer: atgtcgtccatcctgcctttcactc; reverse primer: ctaagacacactggaacagcggatg. Nucleotide substitution A607G(silent) to NM_005902.3.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human SMAD3 cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank

External Database
Human SMAD3

          Reference sequence
            
           
          Remarks, protocol and/or map (pdf) RDB07019.pdf

          Distribution information

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          RDB07019 pCMFlag_hsSMAD3 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCMFlag_hsSMAD3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB07019).

          Reference section:

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB07019_A7B6p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB07019_A7B6a.seq check
          >07019_07019_A7B6_CMV-Forward_F04_16_ABI24.ab1
              1 CGTCCTCCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
             61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
            121 CACTATAGGG AGACCCAAGC TGAATTCACC ATGGACTACA AGGACGATGA CGATAAGGCG
            181 GCCATGTCGT CCATCCTGCC TTTCACTCCC CCGATCGTGA AGCGCCTGCT GGGCTGGAAG
            241 AAGGGCGAGC AGAACGGGCA GGAGGAGAAA TGGTGCGAGA AGGCGGTCAA GAGCCTGGTC
            301 AAGAAACTCA AGAAGACGGG GCAGCTGGAC GAGCTGGAGA AGGCCATCAC CACGCAGAAC
            361 GTCAACACCA AGTGCATCAC CATCCCCAGG TCCCTGGATG GCCGGTTGCA GGTGTCCCAT
            421 CGGAAGGGGC TCCCTCATGT CATCTACTGC CGCCTGTGGC GATGGCCAGA CCTGCACAGC
            481 CACCACGAGC TGCGGGCCAT GGAGCTGTGT GAGTTCGCCT TCAATATGAA GAAGGACGAG
            541 GTCTGCGTGA ATCCCTACCA CTACCAGAGA GTAGAGACAC CAGTTCTACC TCCTGTGTTG
            601 GTGCCACGCC ACACAGAGAT CCCGGCCGAG TTCCCCCCAC TGGACGACTA CAGCCATTCC
            661 ATCCCCGAAA ACACTAACTT CCCCGCAGGC ATCGAGCCCC AGAGCAATAT TCCAGAGACC
            721 CCACCCCCTG GCTACCTGAG TGAAGATGGA GAAACCAGTG ACCACCAGAT GAACCACAGC
            781 ATGGACGCAG GTTCTCCAAA CCTATCCCCG AATCCGATGT CCCCAGCACA TAATAACTTG
            841 GACCTGCAGC CAGTTACCTA CTGCGAGCCG GCCTTCTGGT GCTCCATCTC CTACTACGAG
            901 CTGAACCAGC GCGTCGGGGA GACATTCCAC GCCTCGCAGC CATCCATGAC TGTGGATGGC
            961 TTCACCGACC CCTCCCAATT CGGAGCGCTT TCTGCCCTAG GGCTGCTCTC CCAAATGTCA
           1021 ACAGGAAATG CAGCAGTGGG AGCTGGACAC GGAGGACACA TCGCGAGCCG TGCCGACTCT
          1081 ACCTACAATC GGAAGGGGAA GGG
          //
          Primer: BGH_rev2
          Sequence file: RDB07019_A7B6b.seq check
          >07019_07019_A7B6_BGH_rev2_E10_13_ABI24.ab1
              1 CGGTTCGGGA TAGAGGCACG GGGGAGGGGC AACAACAGAT GGCTGGCAAC TAGAAGGCAC
             61 AGTCGAGGCT GATCAGCGAG CTCTAGCATT TAGGTGACAC TATAGAATAG GGCCCTCTAG
            121 ATGCATGCTC GAGCGGCCCT AAGACACACT GGAACAGCGG ATGCTTGGGG AGCCCATCTG
            181 GGTGAGGACC TTGTCAAGCC ACTGCAAAGG CCCATTCAGG TGCAGCTCAA TCCAGCAGGG
            241 GGTACTGGTC ACAGTCTGTC TCCTGTACTC CGCTCCCCAG CCTTTGACGA AGCTCATGCG
            301 GATGGTGCAC ATTCGGGTCA ACTGGTAGAC AGCCTCAAAG CCCTGGTTGA CCGACTGGGC
            361 CAGGAGGGCA GCGAACTCCT GGTTGTTGAA GATCTTCAGG TTGCATCCTG GTGGGATCTT
            421 GCAGACGGTG GCCGGGTGCC AGCCATAGCG CTGGTTACAG TTGGGAGACT GGACAAAAAT
            481 AGCGCTGTCA CTGAGGCACT CTGCGAAGAC CTCCCCTCCG ATGTAGTAGA GCCGCACGCC
            541 TCTTCCGATG TGTCTCCGTG TCAGCTCCAC TGCTGCATTC CTGTTGACAT TGGAGAGCAG
            601 CCCTAGGCAG AAGCGCTCCG AATTGGAGGG GTCGGTGAAG CCATCCACAG TCATGGATGG
            661 CTGCGAGGCG TGGAATGTCT CCCCGACGCG CTGGTTCAGC TCGTAGTAGG AGATGGAGCA
            721 CCAGAAGGCC GGCTCGCAGT AGGTAACTGG CTGCAGGTCC AAGTTATTAT GTGCTGGGGA
            781 CATCGGATTC GGGGATAGGT TTGGAGAACC TGCGTCCATG CTGTGGTTCA TCTGGTGGTC
            841 ACTGGTTTCT CCATCTTCAC TCAGGTAGCC AGGGGGTGGG GTCTCTGGAA TATTGCTCTG
            901 GGGCTCGATG CCTGCGGGGA AAGTTAGTGT TTTCGGGGAT GGAATGGCTG TAGTCGTCCA
            961 GTGGGGGGAA ACTCGGGCCG GGATCTCTGT GTGGCGTGGC ACCAACACAG GAGGTAGAAC
           1021 TGGTTGTCTC TACTCTCTGG GTAGTGGTAG GATTTCACGG CAGACCTCGT CCTTCCTTCC
          1081 ATATGAGCCG AGCTCACCAC AGCTCCATGG GCCCCCGCCA AG
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB07019_A7B6c.seq check
          >07019_07019_A7B6_SV40pro_F_V2_F10_16_ABI24.ab1
              1 GGGCGAGCTT TTTGGGAGGC TAGGCTTTTG CAAAGCTCCC GGGCAGCTTG GATATCCATT
             61 TTCGGATCTG ATCAAGAGAC AGGATGAGGA TCGTTTCGCA TGATTGAACA AGATGGATTG
            121 CACGCAGGTT CTCCGGCCGC TTGGGTGGAG AGGCTATTCG GCTATGACTG GGCACAACAG
            181 ACAATCGGCT GCTCTGATGC CGCCGTGTTC CGGCTGTCAG CGCAGGGGCG CCCGGTTCTT
            241 TTTGTCAAGA CCGACCTGTC CGGTGCCCTG AATGAACTGC AGGACGAGGC AGCGCGGCTA
            301 TCGTGGCTGG CCACGACGGG CGTTCCTTGC GCAGCTGTGC TCGACGTTGT CACTGAAGCG
            361 GGAAGGGACT GGCTGCTATT GGGCGAAGTG CCGGGGCAGG ATCTCCTGTC ATCTCACCTT
            421 GCTCCTGCCG AGAAAGTATC CATCATGGCT GATGCAATGC GGCGGCTGCA TACGCTTGAT
            481 CCGGCTACCT GCCCATTCGA CCACCAAGCG AAACATCGCA TCGAGCGAGC ACGTACTCGG
            541 ATGGAAGCCG GTCTTGTCGA TCAGGATGAT CTGGACGAAG AGCATCAGGG GCTCGCGCCA
            601 GCCGAACTGT TCGCCAGGCT CAAGGCGCGC ATGCCCGACG GCGAGGATCT CGTCGTGACC
            661 CATGGCGATG CCTGCTTGCC GAATATCATG GTGGAAAATG GCCGCTTTTC TGGATTCATC
            721 GACTGTGGCC GGCTGGGTGT GGCGGACCGC TATCAGGACA TAGCGTTGGC TACCCGTGAT
            781 ATTGCTGAAG AGCTTGGCGG CGAATGGGCT GACCGCTTCC TCGTGCTTTA CGGTATCGCC
            841 GCTCCCGATT CGCAGCGCAT CGCCTTCTAT CGCCTTCTTG ACGAGTTCTT CTGAGCGGGA
            901 CTCTGGGGGT TCGAAATGAC CGACCAAGCG ACGCCCAACC CTGCCATCAC GAGATTTCGA
            961 TTCCCACCGC CCGCCTTCTA TGAAAGGTTG GGCTTTCGGA ATCGTTTTCC CGGGACGCCC
           1021 GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGGAG CTCTTCGGCC CACCCCCAAC
          1081 TTGATTATTG GCAGCTATAT TGCTTACGAA TAAAGCCAAA TAGGCAATTC CCACCGA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          reference Katanasaka, Y., Fibroblast-specific PRMT5 deficiency suppresses pressure overload-induced cardiac fibrosis and left ventricular dysfunction Research Square Nov 29; https://doi.org/10.21203/rs.3.rs-2299878/v1
          user_report Yuki, R., Desuppression of TGF-beta signaling via nuclear c-Abl-mediated phosphorylation of TIF1 gamma/TRIM33 at Tyr-524, -610, and -1048. Oncogene 38 (5): 637-655 (2019). PMID 30177833.
          user_report Hirata, K., Forkhead box protein A1 confers resistance to transforming growth factor-beta-induced apoptosis in breast cancer cells through inhibition of Smad3 nuclear translocation. J. Cell. Biochem. 120 (2): 2259-2270 (2019). PMID 30206966.

          2023.05.22

          GNP_filter3_RDBDEP_html_230512.pl