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pCMFlag_hsSMAD2

Expression vector of human SMAD2.

Catalog number RDB07000
Resource name pCMFlag_hsSMAD2
Alternative name SET-0209_1-1
Clone info. Expression vector of human SMAD2. Expression has not yet been confirmed.
Comment Derived from Sugano CAS09876. PCR cloning, forward primer: atgtcgtccatcttgccattcacg; reverse primer: ttatgacatgcttgagcaacgcact. cDNA fragment 293 to 1696nt of NM_005901.5 (cds 293-1696nt) was cloned. Known difference: T to C substitution at 583nt (silent).
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human MADH2/SMAD2 cDNA
Depositor|Developer DNA Bank, |
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External Database
Human MADH2/SMAD2

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
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          提供条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB07000 pCMFlag_hsSMAD2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14573_A7B6p1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB14573_A7B6a.seq check
          >07000_14573_A7B6_CMV-Forward_A11_02_ABI24.ab1
              1 CTGTCACTCC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
             61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
            121 CTCACTATAG GGAGACCCAA GCTGAATTCA CCATGGACTA CAAGGACGAT GACGATAAGG
            181 CGGCCATGTC GTCCATCTTG CCATTCACGC CGCCAGTTGT GAAGAGACTG CTGGGATGGA
            241 AGAAGTCAGC TGGTGGGTCT GGAGGAGCAG GCGGAGGAGA GCAGAATGGG CAGGAAGAAA
            301 AGTGGTGTGA GAAAGCAGTG AAAAGTCTGG TGAAGAAGCT AAAGAAAACA GGACGATTAG
            361 ATGAGCTTGA GAAAGCCATC ACCACTCAAA ACTGTAATAC TAAATGTGTT ACCATACCAA
            421 GCACTTGCTC TGAAATTTGG GGACTGAGTA CACCAAATAC GATAGATCAG TGGGACACAA
            481 CAGGCCTTTA CAGCTTCTCT GAACAAACCA GGTCTCTTGA TGGTCGTCTC CAGGTATCCC
            541 ATCGAAAAGG ATTGCCACAT GTTATATATT GCCGATTATG GCGCTGGCCT GATCTTCACA
            601 GTCATCATGA ACTCAAGGCA ATTGAAAACT GCGAATATGC TTTTAATCTT AAAAAGGATG
            661 AAGTATGTGT AAACCCTTAC CACTATCAGA GAGTTGAGAC ACCAGTTTTG CCTCCAGTAT
            721 TAGTGCCCCG ACACACCGAG ATCCTAACAG AACTTCCGCC TCTGGATGAC TATACTCACT
            781 CCATTCCAGA AAACACTAAC TTCCCAGCAG GAATTGAGCC ACAGAGTAAT TATATTCCAG
            841 AAACGCCACC TCCTGGATAT ATCAGTGAAG ATGGAGAAAC AAGTGACCAA CAGTTGAATC
            901 AAAGTATGGA CACAGGCTCT CCAGCAGAAC TATCTCCTAC TACTCTTTCC CCTGTTAATC
            961 ATAGCTTGGA TTTACAGCCC AGTTTACTTA CTCAGACCTG CATTTTGGTG TTCGATAGCA
           1021 TATTATGATT TAAATCAGAG GTTGGGAGAA ACCTTTCATG CATCACAGCC CTCCACTTCA
          1081 CTGTAGATTG CCTTTACGGA CCATTCCAAA ATTCAAGAAG AAAGGATTC
          //
          Primer: BGH_rev2
          Sequence file: RDB14573_A7B6b.seq check
          >07000_14573_A7B6_BGH_rev2_B11_05_ABI24.ab1
              1 CGTTTCGCGC TCAGGACGCA CGGGGGAGGG GCAACAACAG ATGGCTGGCA ACTAGAAGGC
             61 ACAGTCGAGG CTGATCAGCG AGCTCTAGCA TTTAGGTGAC ACTATAGAAT AGGGCCCTCT
            121 AGATGCATGC TCGAGCGGCC TTATGACATG CTTGAGCAAC GCACTGAAGG GGATCCCATC
            181 TGAGTTAATA CTTTGTCCAA CCACTGTAGA GGTCCATTCA GATGAAGTTC AATCCAGCAA
            241 GGAGTACTTG TTACCGTCTG CCTTCGGTAT TCTGCTCCCC ACCCTTTCAC AAAACTCATT
            301 CTTATGGTGC ACATTCTAGT TAGCTGATAG ACGGCTTCAA AACCCTGATT AACAGACTGA
            361 GCCAGAAGAG CAGCAAATTC CTGGTTGTTG AAGATCTTCA GATTACAGCC TGGTGGAATT
            421 TTACACACTG TTGCAGGGTG CCAGCCATAT CTCTGATTAC AATTGGGGCT CTGCACAAAG
            481 ATTGCACTAT CACTTAGGCA CTCAGCAAAA ACTTCCCCAC CTATGTAGTA TAAGCGCACT
            541 CCTCTTCCTA TATGCCTTCT TGTCATTTCT ACCGTGGCAT TTCGGTTAAC ATTGGAGAGT
            601 AAACCTAAGC AGAACCTCTC TGAATTTGAT GGGTCTGTAA AGCCATCTAC AGTGAGTGAG
            661 GGCTGTGATG CATGGAAGGT TTCTCCAACC CTCTGATTTA ATTCATAATA TGCTATCGAA
            721 CACCAAAATG CAGGTTCTGA GTAAGTAACT GGCTGTAAAT CCAAGCTATG ATTAACAGGG
            781 GAAAGAGTAG TAGGAGATAG TTCTGCTGGA GAGCCTGTGT CCATACTTTG ATTCAACTGT
            841 TGGTCACTTG TTTCTCCATC TTCACTGATA TATCCAGGAG GTGGCGTTTC TGGAATATAA
            901 TTACTCTGTG GCTCAATTTC CTGCTGGGAA GTTAGTGTTT TTCTGGAATG GGAGTGAGTA
            961 TAGTCATCCA GAGGCCGGAA GTTTCTGTTA GAATCTCCGG TGTGTCGGGG CACTTAATAC
           1021 TGGAGGCAAA ACTTGCTGTC CTCACTCCTC TGATAGTGGG TTAGGGCTTA CGCATACCGT
           1081 TCAATCCCTT TTTTAGGATG AAAGCACATA TCGCCCAGGC TTTTCCAGAG TTGCCTTTGT
          1141 GAGG
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB14573_A7B6c.seq check
          >07000_14573-A7B6_SV40proF_V2_E04_13_ABI24.ab1
              1 GGACCAAGCT TTTTTGGAGG GCTAGGCTTT TGCAAAAGCT CCCGGGAGCT TGGATATCCA
             61 TTTTCGGATC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT
            121 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC
            181 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC
            241 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC
            301 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG
            361 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC
            421 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG
            481 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC
            541 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC
            601 CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA
            661 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA
            721 TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG
            781 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG
            841 CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAGCGG
            901 GACTCTGGGG GTTCGAAATG ACCGACCCAA GCGACGCCCA ACCCTGCCCA TCACGAGATT
            961 TCGATTCCAC CGCCCGCCTT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGACGCC
           1021 GGCTGGATGA TCCTCAGCGC GGGATCTCAT GCCTGAGTCC TCGGCCAACC CCAACTGTAA
          1081 TGGCAGCTTA ATATGTACAA ATTAAGGCAA TATAAGGC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl