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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMFlag_hsCCNH

Expression vector of human CCNH.

Catalog number RDB06416
Resource name pCMFlag_hsCCNH
Alternative name SET-0192_1
Clone info. Expression vector of human CCNH. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment Derived from Sugano CAS09072. PCR cloning, forward primer: atgtaccacaacagtagtcagaagc; reverse primer: ttagagagattctaccaggtcgtc.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human CCNH/Cyc H cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human CCNH/Cyc H
          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to describe RIKEN BRC as the source of the MATERIAL and cite Gene 200, 149-156, 1997 in any publication.
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          提供条件 発表に際し、理研BRCから入手したことを明記し、指定する文献(Gene, 200, 149-156 (1997))を引用すること。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB06416 pCMFlag_hsCCNH DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (pdf) RDB06416.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12340_A3Li.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer:
          Sequence file: RDB12340_A3Lia.seq check
          >06416_12340_A3Li_CMV-Foward_A04_01.ab1
              1 CGGTTCCACC TCCCGCCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
             61 TATATAAGCA GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT
            121 AATACGACTC ACTATAGGGA GACCCAAGCT GAATTCACCA TGGACTACAA GGACGATGAC
            181 GATAAGGCGG CCATGTACCA CAACAGTAGT CAGAAGCGGC ACTGGACCTT CTCCAGCGAG
            241 GAGCAGCTGG CAAGACTGCG GGCTGACGCC AACCGCAAAT TCAGATGCAA AGCCGTGGCC
            301 AACGGGAAGG TTCTTCCGAA TGATCCAGTC TTTCTTGAGC CTCATGAAGA AATGACACTC
            361 TGCAAATACT ATGAGAAAAG GTTATTGGAA TTCTGTTCGG TGTTTAAGCC AGCAATGCCA
            421 AGATCTGTTG TGGGTACGGC TTGTATGTAT TTCAAACGTT TTTATCTTAA TAACTCAGTA
            481 ATGGAATATC ACCCCAGGAT AATAATGCTC ACTTGTGCAT TTTTGGCCTG CAAAGTAGAT
            541 GAATTCAATG TATCTAGTCC TCAGTTTGTT GGAAACCTCC GGGAGAGTCC TCTTGGACAG
            601 GAGAAGGCAC TTGAACAGAT ACTGGAATAT GAACTACTTC TTATACAGCA ACTTAATTTC
            661 CACCTTATTG TCCACAATCC TTACAGACCA TTTGAGGGCT TCCTCATCGA CTTAAAGACC
            721 CGCTATCCCA TATTGGAGAA TCCAGAGATT TTGAGGAAAA CAGCTGATGA CTTTCTTAAT
            781 AGAATTGCAT TGACGGATGC TTACCTTTTA TACACACCTT CCCAAATTGC CCTGACTGCC
            841 ATTTTATCTA GTGCCTCCAG GGCTGGAATT ACTATGGAAA GTTATTTATC AGAGAGTCTG
            901 ATGCTGAAAG AGAACAGAAA CTTGCCTGTC ACAGTTACTA GATATAATGA AAAGCATGAG
            961 AAACTTAGTA AAGAAGTATG AACCACCCAG ATCTGAAGAA GTTGCTGTTC TGAAACAGAA
           1021 GTTGGGAGCG ATGTCATTCT GCTGAGCTTG CACTTTAACG TAATCACGAA GAAGAGAAAG
           1081 GGCTATGAAG GATGATGATT TACGTCTCAA AGGAAATCCA AACATGAGGA GAGGATGGAA
           1141 CTGATGACGA CCTGGTAGAT CTCCTCTAAG TCGCTTCGAG CATGCATTCT AGGAGGGCCC
          1201 CATATTTCTC TATT
          //
          Primer:
          Sequence file: RDB12340_A3Lib.seq check
          >06416_12340_A3Li_BGHrev_B04_04.ab1
              1 GTTTGAATAT CCATTTAGGT GAACTATAGA ATAGGGCCCT CTAGATGCAT GCTCGAGCGG
             61 CCTTAGAGAG ATTCTACCAG GTCGTCATCA GTCCATTCTT CCTCCTCATG TTTGGATTTC
            121 TTTGAGACGT AATCATCATC TTCATAGCCT TTCCTCTTCT TCGTGATTAC GTTAAGTGCA
            181 AGCTCAGCAG AATGACATCG CTCCAACTTC TGTTTCAGAA CAGCAACTTC TTCAGATCTG
            241 GGTGGTTCAT ACTTCTTTAC TAAGTTTCTC ATGCTTTTCA TTATATCTAG TAACTGTGAC
            301 AGGCAAGTTC TGTTCTCTTT CAGCATCAGA CTCTCTGATA AATAACTTTC CATAGTAATT
            361 CCAGCCCTGG AGGCACTAGA TAAAATGGCA GTCAGGGCAA TTTGGGAAGG TGTGTATAAA
            421 AGGTAAGCAT CCGTCAATGC AATTCTATTA AGAAAGTCAT CAGCTGTTTT CCTCAAAATC
            481 TCTGGATTCT CCAATATGGG ATAGCGGGTC TTTAAGTCGA TGAGGAAGCC CTCAAATGGT
            541 CTGTAAGGAT TGTGGACAAT AAGGTGGAAA TTAAGTTGCT GTATAAGAAG TAGTTCATAT
            601 TCCAGTATCT GTTCAAGTGC CTTCTCCTGT CCAAGAGGAC TCTCCCGGAG GTTTCCAACA
            661 AACTGAGGAC TAGATACATT GAATTCATCT ACTTTGCAGG CCAAAAATGC ACAAGTGAGC
            721 ATTATTATCC TGGGGTGATA TTCCATTACT GAGTTATTAA GATAAAAACG TTTGAAATAC
            781 ATACAAGCCG TACCCACAAC AGATCTTGGC ATTGCTGGCT TAAACACCGA ACAGAATTCC
            841 AATAACCTTT TCTCATAGTA TTTGCAGAGT GTCATTTCTT TCATGAGGCT CAAGAAAGAC
            901 TGGATCATTC GGAAGAACCT TCCCGTTGGC CACGGCTTTG CATCTGAATT TGCGGTTGGC
            961 GTCAGCCCGC AGTCTTGCCA GCTGCTCCTC GCTGGAGAAA GGTCCAGTGC CGCTTCTGAC
           1021 TACTGTTGTG GTACATGGCC GCCTTATCGT CATCGTCCCT TGTAGTCCAT GGTGAAATTC
           1081 AGCTTGGTCT CCCTATAGTG AGTCGTATAA TTCGATAAGC CCAGTAAGGC AGTGGGTTCT
           1141 CTAGTAGGCC AGAGAAGCTC TGCTATATTA GACCTCCCAC GTACACTGCC TTCACGGCCA
          1201 ATTGTGCCTG TCTCCTAG
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl