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pCMFlag_hsFOSL1

Expression vector of human FOSL1.

Catalog number RDB06261
Resource name pCMFlag_hsFOSL1
Alternative name SET-0037_8
Clone info. Expression vector of human FOSL1. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR cloning, forward primer: atgttccgagacttcgggga; reverse primer: tcacaaagcgaggagggttg.
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human Fra1; FOSL1 cDNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human Fra1; FOSL1
          Reference sequence
            
           
          Remarks, protocol and/or map (pdf) RDB06261.pdf

          Distribution information

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          RDB06261 pCMFlag_hsFOSL1 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCMFlag_hsFOSL1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06261).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18651_B1Aip1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: CMV pro,FLAG,insert 5'
          Sequence file: RDB18651_B1Aia.seq check
          >06261_18651_B1Ai_1_CMV_Forward_A02_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNATG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
             61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
            121 ACGACTCACT ATAGGGAGAC CCAAGCTGAA TTCACCATGG ACTACAAGGA CGATGACGAT
            181 AAGGCGGCCA TGTTCCGAGA CTTCGGGGAA CCCGGCCCGA GCTCCGGGAA CGGCGGCGGG
            241 TACGGCGGCC CCGCGCAGCC CCCGGCCGCA GCGCAGGCAG CCCAGCAGAA GTTCCACCTG
            301 GTGCCAAGCA TCAACACCAT GAGTGGCAGT CAGGAGCTGC AGTGGATGGT ACAGCCTCAT
            361 TTCCTGGGGC CCAGCAGTTA CCCCAGGCCT CTGACCTACC CTCAGTACAG CCCCCCACAA
            421 CCCCGGCCAG GAGTCATCCG GGCCCTGGGG CCGCCTCCAG GGGTACGTCG AAGGCCTTGT
            481 GAACAGATCA GCCCGGAGGA AGAGGAGCGC CGCCGAGTAA GGCGCGAGCG GAACAAGCTG
            541 GCTGCGGCCA AGTGCAGGAA CCGGANGAAG GAACTGACCG ACNTCCTGCN NGNGGANACT
            601 GACAAACTGG AAGANGAGAA NNCTGGGCTG CNGCGAGAGN NTGANGANCT GCAGAANCAG
            661 ANNNNCGCCN ANANCTGGNG CTNGGANNCC CNNCNNACCN NNNTGNNAAN NNCNNGNNNN
          721 NNCNNNNNNN GGGGANNNNN NNNN
          //
          Primer: bGH_rev3 (Pr0885)
          Region: bGHpA,insert 3'
          Sequence file: RDB18651_B1Aib.seq check
          >06261_18651_B1Ai_1_bGH_rev3_B02_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNGNNGN NGGGCNNACA ACAGATGGCT GGCAACTAGA AGGCACAGTC
             61 GAGGCTGATC AGCGAGCTCT AGCATTTAGG TGACACTATA GAATAGGGCC CTCTAGATGC
            121 ATGCTCGAGC GGCCTCACAA AGCGAGGAGG GTTGGAGAGC CAAGGGGGTC AGAGGATGGG
            181 TCTCCGCTGC TGCTGCTACT CTTGCGATGA GCTGAGGCAC AAGGCTCAGG AGTGCTGGGG
            241 TAGGTGAAGA CCAGGCTGGG GGTGAAAGGA GTTAGGGAGG GTGTGGTCAT GAGTGTGGGG
            301 GTGTGCAGTG CCTCAGGTTC AAGCACAGGC CCTGGGGAAA GGGAGATACA AGGTACAGGG
            361 CGGCAGGGGG CTGGTGGGCT GCTGGTGCCA CTGGTACTGC CTGTGTCCCC CTCCTTGGCT
            421 CCTTCCGGGA TTTTGCAGAT GGGTCGGTGG GCTTCCAGCA CCAGCTCTAG GCGCTCCTTC
            481 TGCTTCTGCA GCTCCTCAAT CTCTCGCTGC AGCCCANATT TCTCATCTTC CAGTTTGTCA
            541 NNCTCCGCCT GCAGGAAGTC GGTCAGTTCC TTCCTCCGGN TCCTGCACTT GNCCGCANCC
            601 AGCTTGTTCC GCTNNNNNNT TANNNNNNGG CGCTCCTCTT NCNNCGGGCT GATCTGNNNN
            661 NNAGGNNNNN NACNNNNNNN TGNNNGCNGN CNCNNNNNNC NNNNNNNCTC CNGNNNNNNN
          721 NNN
          //
          Primer: SV40pro_ori_F (Pr0731)
          Region: SV40 pro_ori,NeoR
          Sequence file: RDB18651_B1Aic.seq check
          >06261_18651_B1Ai_1_SV40pro_ori_F_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NCTNNNNNNC TGNNCTATTC NNGAAGTAGT GAGGAGGCTT
             61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGGATATCCA TTTTCGGATC
            121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG
            181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG
            241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA
            301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT
            361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA
            421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC
            481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CNTACGCTTG ATCCGGCTAC
            541 CTGCCCATTC GACCACCNAN GCGAAACATC GCATCGAGCG AGCACNTACT CGGATGGAAN
            601 CCGGTCTNGN NNNTCANGAN GATNNGGACN AANANCNTCN NGGCTCGCGC CNNCCNNACT
            661 GTTCGCNNGG CTNNAGGCGC GNATGCCCNA NNNNCNAAGG NNCTCNTCNT GNCCCNNNGN
          721 GANNCNNNNN NNNNCNNAAN NNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl