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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCMFlag_lacZ

Expression vector of lacZ, with FLAG-tag, control vector

Catalog number RDB06072
Resource name pCMFlag_lacZ
Alternative name SET-0109_5
Clone info. Expression vector of E. coli lacZ. Expression was confirmed by Western blotting with anti-FLAG antibody.
Comment PCR template： pcDNA4/HisMax/lacZ
Vector backbone pCMV_S-FLAG (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name E. coli lacZ (beta-D-galactosidase)
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
E. coli lacZ (beta-D-galactosidase)
          Reference sequence
            
           
          Sequence (full) RDB06072zzt01.seq compiled by us
          Remarks, protocol and/or map (pdf) RDB06072.pdf

          Distribution information

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          RDB06072 pCMFlag_lacZ DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pCMFlag_lacZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06072).

          Reference section:

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content pCMV_tag and pRSV_tag assay-ready clones (English text)
          Featured content Arranged vector (English text)
          Featured content pCMV_tag and pRSV_tag vector (English text)
          Featured content pCMV_tag and pRSV_tag vector (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14342_A6Inp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward (Pr0016)
          Region: FLAG,insert
          Sequence file: RDB14342_A6Ind.seq check
          >06072_14342_A6In_CMV-Forward_H10_22_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGN NGCNNATGGG CGGTANGCGT GTACGGTGGG AGGTCTATAT
             61 AAGCAGAGCT CTCTNGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
            121 GACTCACTAT AGGGAGACCC AAGCTGAATT CACCATGGAC TACAAGGACG ATGACGATAA
            181 GGCGGCCGAT CCCGTCGTTT TACAACGTCG TGACTGGGAA AACCCTGGCG TTACCCAACT
            241 TAATCGCCTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGCAC
            301 CGATCGCCCT TCCCAACAGT TGCGCAGCCT GAATGGCGAA TGGCGCTTTG CCTGGTTTCC
            361 GGCACCAGAA GCGGTGCCGG AAAGCTGGCT GGAGTGCGAT CTTCCTGAGG CCGATACTGT
            421 CGTCGTCCCC TCAAACTGGC AGATGCACGG TTACGATGCG CCCATCTACA CCAACGTGAC
            481 CTATCCCATT ACGGTCAATC CGCCGTTTGT TCCCACGGAG AATCCGACGG GTTGTTACTC
            541 GCTCACATTT AATGTTGATG AAAGCTGGCT ACAGGAAGGC CAGACGCGAA TTATTTTTGA
            601 TGGCGTTAAC TCGGCGTTTC ATCTGTGGTG CAACGGGCGC TGGGTCGGTT ACGGCCAGGA
            661 CAGTCGTTTG CCGTCTGAAT TTGACCTGAG CGCATTTTTA CGCGCCGGAN AAAACCGCCT
            721 CGCGGTGATG GTGCTGCGCT GGAGTGACGG CAGTTATCTG GAAGATCAGG ATATGTGGCG
            781 GANGANCGGC ATTTTCCGTG ACGTCTCGTT GCTGCATNAA NCGACTACAC AAATCANCGA
            841 TTTCCNTGTN GCCACTCGCT TNNNTGATGA NTNCAGNCGC NCNNNACNNG ANGNNGNNGT
          901 NCANNNGNNC NGNGAGTTNC NNNNNNNCNN ANNNNNANNN NNTNNNTNNN NNNNNNN
          //
          Primer: BGH_rev (Pr0009)
          Region: insert
          Sequence file: RDB14342_A6Ine.seq check
          >06072_14342_A6In_BGH-rev_A11_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNTAN NNNAGGGCCC TCTAGANGCA TGCTCGAGCG GCCTTATTTT
             61 TGACACCAGA CCAACTGGTA ATGGTAGCGA CCGGCGCTCA GCTGTAATTC CGCCGATACT
            121 GACGGGCTCC AGGAGTCGTC TCCACCAATC CCCATATGGA AACCGTCGAT ATTCAGCCAT
            181 GTGCCTTCTT CCGCGTGCAG CAGATGGCGA TGGCTGGTTT CCATCAGTTG CTGTTGACTG
            241 TAGCGGCTGA TGTTGAACTG GAAGTCGCCG CGCCACTGGT GTGGGCCATA ATTCAATTCG
            301 CGCGTCCCGC AGCGCAGACC GTTTTCGCTC GGGAAGACGT ACGGGGTATA CATGTCTGAC
            361 AATGGCAGAT CCCAGCGGTC AAAACAGGCG GCAGTAAGGC GGTCGGGATA GTTTTCTTGC
            421 GGCCCTAATC CGAGCCAGTT TACCCGCTCT GCTACCTGCG CCAGCTGGCA GTTCAGGCCA
            481 ATCCGCGCCG GATGCGGTGT ATCGCTCGCC ACTTCAACAT CAACGGTAAT CGCCATTTGA
            541 CCACTACCAT CAATCCGGTA GGTTTTCCGG CTGATAAATA AGGTTTTCCC CTGATGCTGC
            601 CACGCGTGAG CGGTCGTAAT CAGCACCGCG TCAGCAAGTG TATCTGCCGT GCACTGCAAC
            661 AACGCTGCTT CGGCCTGGTA ATGGCCCGCC GCCTTCCAGC GTTCGACCCA GGCGTTAGGG
            721 TCAATGCGGG TCGCTTCACT TACGCCAATG TCGTTATCCA GCGGTGCACG GGTNNACTGN
            781 TCGCGCAGCG GCGTCAGCAG TTGTTTTTTT ATCGCNNATC CACATCTGTG AAANAAAGCC
            841 NGANNGGCNG TTAAANTGCC AACGCTNANT ACCNANCTCN ATGCNAAANT CCNTTTCGCN
          901 NGNGGNCANN NGCGGNNNGG NGNGGNNNNN NGNGGGNNNC GNCNNNNNNN NNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl