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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pKM2L-phLCK

Promoter Bank clone, Human lck protein tyrosine kinase (upstream promoter) promoter

Catalog number RDB05892
Resource name pKM2L-phLCK
Alternative name p1977-2F
Clone info. PCR amplified human lck protein tyrosine kinase (upstream promoter) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026) (reference: Mol. Cell. Biol., 9, 2173-2180, 1989, PMID:2787474). Cloned fragment: 32248441 to 32251340(NC_000001.11); relatively -25670 to -22770, where +1 corresponds to 1 nt of NM_001042771.2; relatively -2824 to +76, where +1 corresponds to 1 nt of XM_024447046.1.
Comment PCR cloning, forward primer, 1977 F2: 5' tgaagatggaccaagcctgtcacct 3'; reverse primer, 1977 R2: 5' cacatggtcctcctggcctaacctg 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human lck protein tyrosine kinase (upstream promoter) genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human lck protein tyrosine kinase (upstream promoter)

          Reference sequence
            AL121991

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB05892 pKM2L-phLCK DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB05892_A4Kr.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB05892_A4Kra.seq check
          >05892_05892_A4Kr_1_M13_-40_A06_03.ab1
              1 TAGGCGCGCC GGCCGGTTCC AAGCCTTGCA TGCCTGGCAG GTCATTTAAA ATAGGGATAA
             61 CAGGGTAAAT GATTAAGGAT CTCGAGCTCT AAGCTTCACA TATGCATGCA CTAGTGGCTG
            121 AAGATGGACC AAGCCTGTCA CCTGCACCAG GAGCTGCTGG ATGAGGCCGA GCTGGAGCTG
            181 GAGCTGGAGC CCGGGGCCGG CCTAGCCCTG GCCCCGCTGC TGCGGCACCT GGGCCTCACG
            241 CGCATGAACA TCAGCGCCCG GCGCTTCACC CTCTGCTGAG GAACACCTGT GCCCCCCGAC
            301 TCCCCGCCCC CTCTCCCAAT GCCGCTTCCC CTGCCTGCCT GGGAAGAGGA AAGGGAGGGG
            361 TGCCCCAGAG GCACCAGCTC CTGGCGGGGG AGGAGGAACA TTCAGGTTTC TGAGAGCTGA
            421 ATTCCAAGAG TGCAAAACCC CAGCATCCTG TTTCCTCTGC TGACCCAGCT GGGAGGGGGA
            481 GGAGGAGGAG CTCACACCCT CAAACTCCTC AATAAACGCT TTCTCTGGTT CCCATCAAGG
            541 TCTCTTGCAG TCGTTTATTC AGGCCCAAGG CCGCTATCTG AATGTCGCCT GTCACTGGGA
            601 CCACCCTAGT TCACTGCAGG CCCATGGGAG GTGACTTAGG TAAATTGGGC TCCTGAAGAA
            661 TGCAGAATCT GAGAGACATG GGTTCAAAGC CAGCTACCCC GTTTATTTCT TTTTGTTGTT
            721 GTTATTTCTT CTGCTTCTTT TTTTTTTTTT TTTTTGAGGC AGAGTCTCAC TCTGTCACCC
            781 AGGCTGGAAT GCAGTAGCAC AATCTCAGCT CACTGCAACC TCCGCCTCCG GGGATCAAGC
            841 GATTCTCCTG CCTCAGCCTC CCAAGGTAGC TGGGGATTAC AGGCGGCCAC CACCTCGCCT
            901 GGGCTAATTT CCCTTTCTTT TCTTTTCTTT TTTTTTTTTC TTTTTTTTTG AGATGGAATT
            961 TTCACTCTTT TCGCCCAGGC TGGGAGTAGT GGCGCGATCT TTGGGCTCAC TGCAACCTCC
           1021 ACCTTTCTGA ATTCAAGCGA TTCCTCCCTG CCTCAGCCCT CCCAAGTAGC CTGGGAATTA
           1081 CAGGGGGCCA CAACCTCGCC CTAGCTAATT TCCTTTCCTT TGCTTGTCCT TTTTATCTAT
           1141 TGCTATTTTT TTTTGAGAAT GGAAATTTCA CCTCCATTTT TCCGCCCCCA GGGCTGTGGA
          1201 AAAATAAGTG TGGGGGCGCA GCGAATATTC TGTTGTGGCG TCT
          //
          Primer: phRLR2
          Sequence file: RDB05892_A4Krb.seq check
          >05892_05892_A4Kr_1_phRLR2_D06_12.ab1
              1 CGGGGGGGGG ATGCCGGCGA GCTCCGTTGC AGCCCAGTTT CTATTGGTCT CCTTAAACCT
             61 GTCTTGTAAC CTTGATACTT ACCTGCCCAG TGCCTCACGA CCAACTTCTG CAGTACCCGG
            121 GTGAATTCTA CGCGTCGACA GGCCACATGG TCCTCCTGGC CTAACCTGGC TGCCTGGACA
            181 GTCTCAGCTC CCTTCGCCGT CGTAGCCACC ACCTGTCTCC CCGCGCCCAC GTCCCACCCT
            241 GACTTCCTGC CTCCTCCGGA GGGCTCCGCA GCCAACCGGG CCCCAGCAGC CCAACCACAA
            301 CCCTCTAGCA CGCCAAGCTT CCTGCCACCA TAGACCCCTG CCTCTCAGCC CCTGGGGGTC
            361 CCCTACATCT CCAGACCAGG TCCATCTTCC CCTACTGTCC CAGGGTGACA CTGGGAGGTT
            421 TAAAGTTGAG GGGAGGATGT CTGATAGACC CTCCCTTCTG CCCTCCAGGG TGCATCTGGG
            481 CAAGAGATTC AAGGGCAGCC TTTGGGCCCA GTGAGCAGGG GGCAAGCGGA CACACACTCA
            541 GCTTATGGTG AGAGGCAAAC AGCACCTCAG CACCACCGTT GCAGCTGAGA GACCCTTTTG
            601 TTTCTGTCTG GCAGCAAAGG TAGCATCGTT CAGAGTCAGA GGAGAGGACA ATATCCCTGT
            661 TAAATAGCTG GTGAGCAGAC CATGCATGTT ACAGTCAGGT CAGGCCGAGA GAACAATTCC
            721 CATGTCATCT CAGGCATGGA ACAGTAGCTT TATTATAGTC AAGGGTAGGT GAATGGCAGG
            781 CTACCATCCC TCTTACAGAC AGAATGCTGG AAACAGCCTG TTTCAGGAGG CAGACAGGAC
            841 ACCTCCATGT CTTCAGAGGG CATAGTAACG GCCCCTGCTG TAGTCAGAGG CCAGACAACA
            901 CCCATTATCA TGTTGGAAGA GTAGGCAACA GTTCGAATTC AAGTAGAGCA AGGCCCCAGC
            961 CCTTGCAGAG TGGAGGGCTT GCACTATCTC TGCACAGTGA AGACAGGCAA CAGCGCTCAG
           1021 TCTCAAGTGC AGCCAAGACT GTCAGGACTT CTGTTTAAGC CGAGCAGAGA ATTGTCCAGA
           1081 GCACAGCAGC CAGATTCATT CTTGGCCAGC TAGGACCATT ATCTAGCAGT CAGATGGATT
          1141 CAGGGCCTCT AAG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl