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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pKM2L-phBGR2

Promoter Bank clone, Human brain glutamate receptor 2 (HBGR2) promoter

Catalog number RDB05891
Resource name pKM2L-phBGR2
Alternative name p1975-58F
Clone info. PCR amplified human brain glutamate receptor 2 (HBGR2) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 157219521 to 157221033(NC_000004.12); relatively -1207 to +306, where +1 corresponds to 1 nt of NM_000826.4.
Comment PCR cloning, forward primer, 1975 F2: 5' acataaatttcacacgggaagtcca 3'; reverse primer, 1975 R2: 5' aagtagagcatccacaatatacccc 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed. (reference: J. Neurosci., 18, 6723-6739, 1998, PMID:9712644)
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human glutamate receptor, ionotropic, AMPA 2 genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human glutamate receptor, ionotropic, AMPA 2
          Reference sequence
            AC112240

          Distribution information

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          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB05891 pKM2L-phBGR2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB05891_A4Lm.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB05891_A4Lma.seq check
          >05891_05891_A4Lm_3_M13(-40)_E08_14.ab1
              1 CCGGCGTCAG CTTGCATGCC TGCAGGTCAT TTAATAGGGA TAACAGCGGT AATGATTAAG
             61 ATCTCGAGCT CTAAGCTTCA CATATGCATG CACTAGTGGC ACATAAATTT CACACGGGAA
            121 GTCCAGTCAT CCATCTCGCC ATCAATTCTC TCCCTTCAAA CTTTTAATAT CTAACCTCAT
            181 CTTTCTTTCC CTGTGGCCCG CGTGTTCAGT AGAGCGTGGT GGCTTACTTT CTTTAGGCAG
            241 TGATTTTTCG AAACCCCCGG CCAAAACGCG ATGGGGAAAC AGATTTCTAA GTAAGTCTCT
            301 GGGAAAGAAT CTGCATCTCT GTGGCATTCC TACACTAGCT TCATCTTCTA ATCACTTTCC
            361 TCTAATAGCC TTTCCTTAAA CCAGAGTCTC TTGCTTTCAA TCTGCATAAA GCAAGGGCTT
            421 CTAGGAAACC AAGTCTACAT GACTCTACAA CGGGAGGGAT TGCAGTGAAA GCTGCGAGAA
            481 AGACCCGTGC GCTCTGTAAC TAGGCGAGTC AGCATCACAG CCCAATGGAA TATACTCTAA
            541 AGATGGGATT TTCATGCAAA ATATTTAAAA GATTCCATCT TGATCTCTTG TGCGGCTTAA
            601 GCATTTAAAG ATATAACTTG GGGTTCTCCC TGGATCCTGG CTACATCCCA TGAAGCCCAA
            661 GTGCACAATT TTGGAATATT TCCTCCACGG TGCTTTGCTT GCTTGTTTGC TTTCGGCTGG
            721 GTCAGGTGAG GAGGATGACA GGGCTTCTGT ATTGCTAAAT GGGTTTTCAC AGGAGATGGA
            781 AGATAAAAAT CAAAATTATT TGTAACGTAA GACAGCAGGG CCTGGTGAGA GGACGCTTCG
            841 CCGCCAACAA TTAGCAATTC GGCTTCTACA CAGCAGCCGG AGATCAGCTT TGCTGCATTT
            901 GGTCCAGGTT GGAGCATCTC CGCAGCAGCT GCAACAGCCG CACGAAAGGT AGCTCCGGGC
            961 GGGGAGCGAG GCGCTGTCCT CGGTGCTGAA AGGCCGAGGC GCGCGGTGGG CGCGACAGCC
           1021 CCGGAGACCC GAGGTCTCGC GGAGGGACAG CGGCTACGGG CCCCGAGCTG TGCTTTCTCA
           1081 GCGCCGCGCA CGCGACGCGT CACGTTGATG CGGGATGCCG GGCGTCGTGC CGCGGGGGGA
          1141 AAGAGGGGGG GTCTCCTCGA CT
          //
          Primer: phRLR2
          Sequence file: RDB05891_A4Lmb.seq check
          >05891_05891_A4Lm_3_phRLR2_F08_17.ab1
              1 AACTCCGTCT GAAGCCCAGT TTCTATTGGT CTCCTTAAAC CTGTCTTGTA ACCTTGATAC
             61 TTACCTGCCC AGTGCCTCAC GACCAACTTC TGCAGTACCC GGGTGAATTC TACGCGTCGA
            121 CAGGCAAGTA GAGCATCCAC AATATACCCC TTTTTTTTTC CTTTTCCTCC TCTTCCTTCT
            181 TTGGCTGTTT TCCTCTGCAA ATTAGATCCT CTGCATTTTG AGACAGCAAT TTAGCACCAA
            241 GCTGCTAAAA CCTGAAGTGG AGGCAGAAGA ATCCCTATTT CCCAGGTCCT GCTGGTGGCT
            301 GCTGATGCCC TGCGCCCTGT CCCCGCTCCA GAAGTCCGGA GGACTGGCTG AATGCAGTTT
            361 TCAGCGGAGC TGCCGCTGTG GTCCCAGTGT CTCGGAATAT TCAGCACCCT CCCATGCACT
            421 CACACACACT CACCCTCTCT CGCGCTCTCT TCTCCCTCTC TCCTCTCTCA CTCACGCGCG
            481 CGCGCACACA CACACACACA CATACACACA CGCACACACA CAGGCAAGTC ACAGAGAGGG
            541 GCAGGCAGTC CCGGGCGCGC GTGCGCGACT CGAGGCAGGA GGCGGGAGCG CGCGCCCCCT
            601 CCCCCGCACG GCGCCCGGCA CCCCGCACCA CCGTGGACGC GTCGCGTGCG CGGCGCTGAG
            661 AAAGCACAGC TCGGGGCCCG TAGCCGCTGT CCCTCCGCGA GACCTCGGGT CTCCGGGGCT
            721 GTCGCGCCCA CCGCGCGCCT CGGCCTTTCA GCACCGAGGA CAGCGCCTCG CTCCCCGCCC
            781 GGAGCTACCT TCGTGCGGCT GTTGCAGCTG CTGCGGAGAT GCTCCAACCT GGACCAAATG
            841 CAGCAAAGCT GATCTCCGGC TGCTGTGTAG AAGCCGAATT GCTAATTGTT GGCGGCGAAG
            901 CGTCCTCTCA CCAGGCCCTG CTGTCTTACG TTACAAATAA TTTTGATTTT TATCTTCCAT
            961 CTCCTGTGAA AACCCATTTA GCAATACAGA AGCCCTGTCA TCCCTCCTCA CCTGACCCAG
           1021 CCCGAAAGCA AACAGCAAGC AAAGCACCGT GGAAGGAAAT ATTCCAAAAT TGTGCACTTT
           1081 GGGCTTTCAT GGGAATGTAG CAAGGAATCC AGGAGACCCA AGCTATATTC TTTGAATGCT
          1141 TAGCTCGCCA CAGAGAATTC AAGAGAAGTG GGGGA
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl