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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pKM2L-phBMP7

Promoter Bank clone, Human bone morphogenetic protein 7 (BMP7) promoter

Catalog number RDB05887
Resource name pKM2L-phBMP7
Alternative name p1944-3F
Clone info. PCR amplified human bone morphogenetic protein 7 (BMP7) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 57268601 to 57266779(NC_000020.11); relatively -1960 to -137, where +1 corresponds to 1 nt of NM_001719.3.
Comment PCR cloning, forward primer, 1944F-SpeI: 5' gactactagtcctggaggcggctccttgatggag 3'; reverse primer, 1944R-SalI: 5' gactgtcgacctagagcgctcgacaaatgtttac 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed. (reference: Bone, 29, 54-61, 2001, PMID:11472891)
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human bone morphogenetic protein 7 genomic DNA
Depositor|Developer DNA Bank, |
Other clones in our bank
External Database
Human bone morphogenetic protein 7
          Reference sequence
            AF210054

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB05887 pKM2L-phBMP7 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13034_A4La.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB13034_A4Laa.seq check
          >05887_13034_A4La_M13(-40)_D04_10.ab1
              1 CGGCAGAGCC AAGCTTGCAT GCCTGCAGGT CATTTAAATA GGGATAACAG GGTAATGATT
             61 AAGATCTCGA GCTCTAAGCT TCACATATGC ATGCACTAGT CCTGGAGGCG GCTCCTTGAT
            121 GGAGTCTGCC ATGTGGGTTC GCTCATGGCC ATGTCTTCCT GCCCAGCATG GTGCTTGGCC
            181 CTGGGACTGG CCACATAATA TCTGGGCCAG GTGCAAAATT AGTACGGGGC AGGGGGTACT
            241 TTGTTCATAG GTGATTCAGA ACCACATATG GTGACCTCAG AGTAGGAAAC CAAGTGTGGG
            301 GCCCTTAAGA GCTGGGGGGC CCTGTACGAC TGTCCAGGTT GCAGGCCCCA CAGCTCGCCT
            361 CCTGATATCC TGTGCTCCAT GCTTGTCTGT TGAAGGAAGG AGTGAATGGA TGAAGAGCAG
            421 GTGGTGGGGG TGGTTTGAGG GCCTTGCCTG GTGGGTGGGT AGAGGCCCCT CCCTGGCATG
            481 GGGCTCAAGA CCTGTTCCAT CCCACAGCCT GGGGCCTGTG TGTAAATGGC CAGGACCTGC
            541 AGGCTGGCAT TTTTCTGCTC CTTGCCTGGC CTCTGGCCTC CCCTTTCTCC ACCCATGTGG
            601 CCCCTCAGGC TGCCATCTAG TCCAAAAGTC CCCAAGGGAG ACCCAGAGGG CCACTTGGCC
            661 AAACTACTTC TGCTCCAGAA AACTGTAGAA GACCATAATT CTCTTCCCCA GCTCTCCTGC
            721 TCCAGGAAGG ACAGCCCCAA AGTGAGGCTT AGCCAGAGCC CCTCCCAGAC AAGCGCCCCC
            781 GCTTCCCCAA CCTCAGCCCT TCCCAGTTCA TCCCAAAGGC CCTCTGGGGA CCCACTCTCT
            841 CACCCAGCCC CAGGAGGGGA AGGAGACAGG ATGAACTTTT ACCCCGCTGC CCTCACTGCC
            901 ACTCTGGGTG CAGTAATTCC CTTGAGATCC CACACCGGCA GAGGGACCGG TGGGTTCTGA
            961 GTGGTCTGGG GGACTCCCTG TGACAGCGTG CATGGCTCGG TATTGATTGA GGGATGAATG
           1021 GATGAGGAGA GACAGGAGAG GAGGCCCGAT TGGGGAGTCT CAGCACAGAA CCCTTGCAGG
          1081 GCAGAGAATG TGAAGACCAG CCGGCTGCTC CCCCAGGTCC AACTTGGCCC ACCGGAGG
          //
          Primer: phRLR2
          Sequence file: RDB13034_A4Lab.seq check
          >05887_13034_A4La_phRLR2_E04_13.ab1
              1 CTTCTTTTCT GACAGCCCAG TTTCTATTGG TCTCCTTAAA CCTGTCTTGT AACCTTGATA
             61 CTTACCTGCC CAGTGCCTCA CGACCAACTT CTGCAGTACC CGGGTGAATT CTACGCGTCG
            121 ACCTAGAGCG CTCGACAAAT GTTTACTGAG TGCCCACGGG GTGCCTAGAG CCTGGCGGGA
            181 CGTCGCGGGG ATGCGGCTGG AGGAATCGGA GCCGGCCCTG CCCTCTCGGC ACTCGCGGTC
            241 CGGACCAGGA AGGCGGCCCG AGCAGGACGG GTGGGCTGCG GACCGCGGTG GGAAAAGTTG
            301 GGGGAGCGCG GAGGTGGCGC GGAAATAGAA GGCTTCCTGG AAGCGGTGAC CCTTGAGCCG
            361 GTCTTGCCCC TCAGTCCCTG TATCCTTTTA AGCCAGCCAC GCTGACCCCT CCGCGCCTCC
            421 CTGAAGCCGT TGCCAGCCTG TCTCTGCGCT CGCCATTCCC TCCGCCTGGA CCTCTCCCCA
            481 CTCACCACCT CCCGCCCCCA TCAGACGCTG CTTTCTCCTC CCAGAGACCT CCAAGACCTG
            541 CCAAGCTGAG GTTGTTTCCT CGCCTCGCCC CTCGCACGTT TGTTTTCTTT CCTGTAGTTT
            601 CTCTTGCTGT CTGGAATTGC TGTTTGAGTT ACTCCGTGCC TTCTTTATTG CCTTCTTACC
            661 CCCGCTAGAC ACCCAGAGCA CTGGGGCCCG GATCAGCGAT CTTCTCCGCA CCTCTGGTAC
            721 CCGCAGTGCC TTGAACCCAG CGCCTGGTAG CCACTGTAGC TGCGCAGTCA CGATTTGTTG
            781 AGTGAATAAG TGAACTGCGT GTGAGCTGGG GGGGTTCTTC CCACCTCCTC CTCTTCGGGG
            841 CCCTGTATCT TTCCAGCCAC CCCTCAGCCT GAGACCAGAC ACCCCAGCCC CACCACTAGG
            901 GCTTCCCATC AGCCCTCCTC GTGGCAGTGC CTGGGGAGCC AGCCGCTGGT CTTCACATCC
            961 TCTTCCCCTC CAAGGGTCTG TGCCTGAGAC CTCCCCATCG GCCTCCTCTC CTGTCTCTCC
           1021 TCATCCATTC ATCCCTCAAT CAATACCGAG CCATGCCACG CCTGTCACAG GTAGTCCCAG
          1081 ACACTCAGAC CAACCGGATC CCTCTGCCGG GTGTGTGGGA TTCTCTCAAA GGGG
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl