Backbone vector plasmid of shRNA expressing lentivirus construct by Gateway(R) cloning with mRFP1 marker driven by EF-1 alpha promoter.
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Other clones in our bank
External Database Discosoma sp. red fluorescent protein Reference sequence |
Sequence | RDB05736z.seq |
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Remarks, protocol and/or map (pdf) | RDB05736.pdf |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions for distribution | The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer. |
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Ordering | Order form [Credit Card Payment] [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose)[Word] Please visit Ordering instruction.[link] |
Remarks | Remember that you will be working with samples containing infectious virus. Use Survival2 (Invitrogen) or equivalent to amplify this clone. This RESOURCE contains a fluorescent protein provided by Prof. Roger Tsien. UCSD's MTA is also required. Please visit Additional Forms section. This RESOURCE is not provided to for-profit organization or for for-profit research by non-profit organizations. |
提供条件 | 本件研究材料は、非営利機関の非営利学術研究に限って提供する。本件研究材料を利用した研究結果等を発表する際は、本件研究材料が故三好浩之博士により開発されたことを明示する。 |
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提供依頼 | 提供依頼書 [Word] 提供同意書 (MTA, 非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。 手続きの概要は、「レンチウイルスベクターの提供申し込み」をご覧ください。 |
備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 このクローンの増殖にはSurvival2 (Invitrogen)あるいは同等の宿主を使用してください。 Prof. Roger Tsienから供与された蛍光タンパク質を含みます。UCSDとのMTA締結が必要です。「申込みに必要な書式」をご覧ください。本件リソースは営利機関および非営利機関による営利目的研究には提供いたしません。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB05736 | CS-RfA-EF-mRFP1 | DNA solution |
Materials & Methods section:
The CS-RfA-EF-mRFP1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05736). |
Reference section:
Further references such as user reports and related articles (go to bottom)
Featured content
Featured content | Lentiviral plasmid list (English text) |
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Featured content | Lentivirus Vector (English text) |
Featured content | Lentivirus Vector (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB20218_B3Kep1-1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: EF1a-C_F (Pr0168) Region: EF-1alpha pro,mRFP1 5' Sequence file: RDB20218_B3Kea.seq |
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>05736_20218_B3Ke_3_EF1a-C_F_A01_01_ABI24.ab1 1 NNNNNNNNNN NNNNTTGNNT TTGGNNCTTG GTTNNTTCTC NAGCCTCAGA CAGTGGTTCA 61 AAGTTTTTTT CTTCCATTTC AGGTGTCGTG AATTAGATCC GGCGAGGGCA GAGGAAGTCT 121 TCTAACATGC GGTGACGTGG AGGAAAATCC CGGGCCTATG GCCTCCTCCG AGGACGTCAT 181 CAAGGAGTTC ATGCGCTTCA AGGTGCGCAT GGAGGGCTCC GTGAACGGCC ACGAGTTCGA 241 GATCGAGGGC GAGGGCGAGG GCCGCCCCTA CGAGGGCACC CAGACCGCCA AGCTGAAGGT 301 GACCAAGGGC GGCCCCCTGC CCTTCGCCTG GGACATCCTG TCCCCTCAGT TCCAGTACGG 361 CTCCAAGGCC TACGTGAAGC ACCCCGCCGA CATCCCCGAC TACTTGAAGC TGTCCTTCCC 421 CGAGGGCTTC AAGTGGGAGC GCGTGATGAA CTTCGAGGAC GGCGGCGTGG TGACCGTGAC 481 CCAGGACTCC TCCCTGCAGG ACGGCGAGTT CATCTACAAG GTGAAGCTGC GCGGCACCAA 541 CTTCCCCTCC GACGGCCCCG TAATGCAGAA GAAGACCATG GGCTGGGAGG CCTCCACCGA 601 GCGGATGTAC CCCGAGGACG GCGCCCTGAA GGGCGAGATC AAGATGAGGC TGAAGCTGAA 661 GGACGGCGGC CACTACGACG CCGANGTCAA GACCACCTAC ATGGCCAAGA AGCCCGTGCA 721 GCTGCCCGGC GCCTACNAGA NCGACATCAA GCTGGACATC ACCTCCCACA ACGNGGACTA 781 CACCATCGNG GAACAGTACG ANCGCGCCGA GGGCCGCCAC TCCACCGGCG CCTAANCGGN 841 CGCACTCNAN GGNNCNAGCT TATCGATAAT CAANCNCTGG ANNANANNNT TGTGAAAGAN 901 NGANNNGNNN TTCTTNNCNA TGNTGCTCCN TTNNNNCNNN NNNNNNN // |
Primer: env_F (Pr0258) Region: cPPT_CTS,attR1,CmR Sequence file: RDB20218_B3Keb.seq |
>05736_20218_B3Ke_3_env_F_A01_01_ABI08.ab1 1 NNNNNNNNNN NNNNNCNNNN ANNNNNNGNN NCTACAAATG GCAGTATTCA TCCACAATTT 61 TAAAAGAAAA NGNNNNNNNG GGGGGTACAG TGCAGGGGAA AGAATAGTAN NNNNNNNNNC 121 AACAGACATA CAAACTAAAG AATTACAAAA ACAAATTACA AAAATTCAAA ATTTTCGGGT 181 TTATTACAGG GACAGCAGAA ATTCACTTTG AAATCACAAG TTTGTACAAA AAAGCTGAAC 241 GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA GATTTTGCAT AAAAAACAGA 301 CTACATAATA CTGTAAAACA CAACATATCC AGTCACTATG GCGGCCGCAT TAGGCACCCC 361 AGGCTTTACA CTTTATGCTT CCGGCTCGTA TAATGTGTGG ATTTTGAGTT AGGATCCGTC 421 GAGATTTTCA GGAGCTAAGG AAGCTAAAAT GGAGAAAAAA ATCACTGGAT ATACCACCGT 481 TGATATATCC CAATGGCATC GTAAAGAACA TTTTGAGGCA TTTCAGTCAG TTGCTCAATG 541 TACCTATAAC CAGACCGTTC AGCTGGANAT TACGGCCTTT TTAAAGACCG TAAAGAAAAA 601 TAAGCACAAG TTTTATCCGG CCTTTATTCA CATTCTTGCC CGCCTGANGA ANGCTCNTCC 661 GGAATTCCGT ANGGCAATGA AAGACGGTGA NCTGGTGANN TGGGNANAGT GNTNACCCNN 721 NGNTNACNCN NNTTTN // |
Primer: PRE_rev (Pr0250) Region: PRE,mRFP1 3' Sequence file: RDB20218_B3Kec.seq |
>05736_20218_B3Ke_3_PRE_rev_B01_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNCANNATA GTTAAGANNA CCAGTCAATC TTTCACAAAT 61 TTTGTAANCC AGAGGTTGAT TATCGATAAG CTTGATCCCT CGAGTGCGGC CGCTTAGGCG 121 CCGGTGGAGT GGCGGCCCTC GGCGCGCTCG TACTGTTCCA CGATGGTGTA GTCCTCGTTG 181 TGGGAGGTGA TGTCCAGCTT GATGTCGGTC TTGTAGGCGC CGGGCAGCTG CACGGGCTTC 241 TTGGCCATGT AGGTGGTCTT GACCTCGGCG TCGTAGTGGC CGCCGTCCTT CAGCTTCAGC 301 CTCATCTTGA TCTCGCCCTT CAGGGCGCCG TCCTCGGGGT ACATCCGCTC GGTGGAGGCC 361 TCCCAGCCCA TGGTCTTCTT CTGCATTACG GGGCCGTCGG AGGGGAAGTT GGTGCCGCGC 421 AGCTTCACCT TGTAGATGAA CTCGCCGTCC TGCAGGGAGG AGTCCTGGGT CACGGTCACC 481 ACGCCGCCGT CCTCGAAGTT CATCACGCGC TCCCACTTGA AGCCCTCGGG GAAGGACAGC 541 TTCAAGTAGT CGGGGATGTC GGCGGGGTGC TTCACGTAGG CCTTGGAGCC GTACTGGAAC 601 TGAGGGGACA GGATGTCCCA GGCGAAGGGC AGGGGGCCGC CCTTGGTCAC CTTCAGCTTG 661 NCGGTCTGGG TGCCCTCGTA NGGGCGGCCC TCGCCCTCGC CCTCNATCTC GAACTCGTGG 721 CCGTTCACGG AGCCCTCCAT GCGCACCTTG AAGCGCATGA ACTCCTTGAT GACGTCCTCG 781 GNNGNNGCCA TANGNCCGGN NNTTTCCTCC ACGTCACNGC ATGTTANAAG ANTTCCTCTG 841 CCNTCNCCGG NNCTNATTCA CGACANCNNN AATNNAAGAA AAAAANNTTT NNNNCACTNT 901 NNGANGNTNN NNNNTGNANC CNNGNTNCNA ANNNNAAANN NGNNNNNNNN CNNNNNNNAN 961 TTNNNTNNNN NNNNNN // |
Primer: EF1a-N_R (Pr0024) Region: attR2,ccdB Sequence file: RDB20218_B3Ked.seq |
>05736_20218_B3Ke_3_EF1a-N_R_D01_10_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNG GCNNNGTGCG CTCTGCCCNC TNNCGGGCAC CGGNNCCTCA 61 CGAAGCTTGN ANNATCACCA CTTTGTACAA GAAAGCTGAA CGAGAAACGT AAAATGATAT 121 AAATATCAAT ATATTAAATT AGATTTTGCA TAAAAAACAG ACTACATAAT ACTGTAAAAC 181 ACAACATATC CAGTCACTAT GGTCGACCTG CAGACTGGCT GTGTATAAGG GAGCCTGACA 241 TTTATATTCC CCAGAACATC AGGTTAATGG CGTTTTTGAT GTCATTTTCG CGGTGGCTGA 301 GATCAGCCAC TTCTTCCCCG ATAACGGAGA CCGGCACACT GGCCATATCG GTGGTCATCA 361 TGCGCCAGCT TTCATCCCCG ATATGCACCA CCGGGTAAAG TTCACGGGAG ACTTTATCTG 421 ACAGCAGACG TGCACTGGCC AGGGGGATCA CCATCCGTCG CCCGGGCGTG TCAATAATAT 481 CACTCTGTAC ATCCACAAAC AGACGATAAC GGCTCTCTCT TTTATAGGTG TAAACCTTAA 541 ACTGCATTTC ACCAGCCCCT GTTCTCGTCA GCAAAAGAGC CGTTCATTTC AATAAACCGG 601 GCGACCTCAG CCATCCCTTC CTGATTTTCC GCTTTCCAGC GTTCGGCACG CAGACGACGG 661 GCTTCATTCT GCATGGTTGT GCTTACCAGA CCGGANATAT TGACATCATA TATGCCTTGA 721 GCAACTGATA GCTGTCGCTG TCAACTGTCA CTGTAATACG CTGCTTCATA GCATACCTCT 781 TTTTTGACAT ACTTCGGGTA TACATATCAG TATATATTCT TATACCGCAA AAATCAGCGC 841 GCAAATACGC ATACNGTTAT CTGGCTTTNA NNAAGCCNGA TCCNCNCNTT TACNCCCNGN 901 CNNGNNNNTC ANCGNANNAN NNNNGNNNNT CANNNNNNNN NN // |
Primer: CMV_Forward (Pr0016) Region: CMV pro,5' LTR,HIV-1 phi Sequence file: RDB20218_B3Kee.seq |
>05736_20218_B3Ke_3_CMV_Forward_E01_13_ABI24.ab1 1 NNNNNNNNNN NNNNTTGNNG CNNNGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG 61 CAGCGCGTTT TGCCTGTACT GGGTCTCTCT GGTTAGACCA GATCTGAGCC TGGGAGCTCT 121 CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG CTTGCCTTGA GTGCTTCAAG 181 TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG ATCCCTCAGA CCCTTTTAGT 241 CAGTGTGGAA AATCTCTAGC AGTGGCGCCC GAACAGGGAC TTGAAAGCGA AAGGGAAACC 301 AGAGGAGCTC TCTCGACGCA GGACTCGGCT TGCTGAAGCG CGCACGGCAA GAGGCGAGGG 361 GCGGCGACTG GTGAGTACGC CAAAAATTTT GACTAGCGGA GGCTAGAAGG AGAGAGATGG 421 GTGCGAGAGC GTCAGTATTA AGCGGGGGAG AATTAGATCG CGATGGGAAA AAATTCGGTT 481 AAGGCCAGGG GGAAAGAAAA AATATAAATT AAAACATATA GTATGGGCAA GCAGGGAGCT 541 AGAACGATTC GCAGTTAATC CTGGCCTGTT AGAAACATCA GAAGGCTGTA GACAAATACT 601 GGGACAGCTA CAACCATCCC TTCAGACAGG ATCAGAAGAA CTTAGATCAT TATATAATAC 661 AGTAGCAACC CTCTATTGTG TGCATCAAAG GATAGAGATA AAAGACACCA AGGAAGCTTT 721 AGACAAGATA GAGGAAGAGC AAAACAAAAG TAAGACCACC GCACAGCAAG CGGCCGCNGA 781 TCTTCAGACC TGGNNGNNGA NNNATGANGG NNAATTGGAG AANTGAATTA TATAAATATN 841 ANGTANTAAA AANTTGANCC NTTANGNNTA NCNCCCNCCA ANGNNAANNN NNNNNNN // |
Primer: bGH_rev3 (Pr0885) Region: bGH pA,3' LTR Sequence file: RDB20218_B3Kef.seq |
>05736_20218_B3Ke_3_bGH_rev3_G01_19_ABI24.ab1 1 NNNNNNNNNN NNNNGGNNGN NGGGNAACAA CAGNTGGCTG GCAACTAGAA GGCACAGTCG 61 AGGCTGATCA GCGGGTTTAA ACGGGCCCTG CTAGAGANTT TCCACACTGA CTAAAAGGGT 121 CTGAGGGATC TCTAGTTACC AGAGTCACAC AACAGACGGG CACACACTAC TTGAAGCACT 181 CAAGGCAAGC TTTATTGAGG CTTAAGCAGT GGGTTCCCTA GTTAGCCAGA GAGCTCCCAG 241 GCTCAGATCT GGTCTAACCA GAGAGACCCA GTACAGTCCG GATGCAGCTC TCGGGCCATG 301 TGATGAAATG CTAGGCGGCT GTCAAACCTC CACTCTAATA CTTCTCTCTC CGGGTCATCC 361 ATCCCATGCA GGCTCACAGG GTGTAACAAG CGGGTGTTCT CTCCTTCATT GGCTTCTTCT 421 ACCTTCTCTT GCTCAACTGG TACTAGCTTG TAGCACCATC CAAAGGTCAG TGGATATCTG 481 ATCCCTGGCC CTGGTGTGTA GTTCTGCCAA TCAGGGAAGT AGCCTTGTGT GTGGTAGATC 541 CACAGATCAA GGATATCTTG TCTTCGTTGG GAGTGAATTA GCCCTTCCAG TCCCCCCTTT 601 TCTTTTAAAA AGTGGCTAAG ATCTACAGCT GCCTTGTAAG TCATTGGTCT TAAAGGTACC 661 TGANGTGTGA CTGGAAAACC CACCTCCTCC TCCTCTTGTG CTTCTAGCCA GGCACAATCA 721 GCATTGGTAG CTGCTGTATT GCTACTTGTG ATTGCTCCAT GTTTTTCTAG GTCTCGACGG 781 TATCGATGCG GGGNNGCGGC CCNAANGNNN NTCCGANTCG TCTGANGGCG AANGNGAANA 841 NGCGGAANAN GCCGCANNNN NNNNNN // |
Primer: RRE-M_R (Pr0651) Region: RRE,HIV-1 phi Sequence file: RDB20218_B3Keg.seq |
>05736_20218_B3Ke_3_RRE-M_R_F01_16_ABI24.ab1 1 NNNNNNNNNN NNANNNNNGC TGTTGCGCCT CANTAGCCCT CAGCAAATTG TTCTGCTGCT 61 GCACTATACC AGACAATAAT TGTCTGGCCT GTACCGTCAG CGTCATTGAC GCTGCGCCCA 121 TAGTGCTTCC TGCTGCTCCC AAGAACCCAA GGAACAAAGC TCCTATTCCC ACTGCTCTTT 181 TTTCTCTCTG CACCACTCTT CTCTTTGCCT TGGTGGGTGC TACTCCTAAT GGTTCAATTT 241 TTACTACTTT ATATTTATAT AATTCACTTC TCCAATTGTC CCTCATATCT CCTCCTCCAG 301 GTCTGAAGAT CAGCGGCCGC TTGCTGTGCG GTGGTCTTAC TTTTGTTTTG CTCTTCCTCT 361 ATCTTGTCTA AAGCTTCCTT GGTGTCTTTT ATCTCTATCC TTTGATGCAC ACAATAGAGG 421 GTTGCTACTG TATTATATAA TGATCTAAGT TCTTCTGATC CTGTCTGAAG GGATGGTTGT 481 AGCTGTCCCA GTATTTGTCT ACAGCCTTCT GATGTTTCTA ACAGGCCAGG ATTAACTGCG 541 AATCGTTCTA GCTCCCTGCT TGCCCATACT ATATGTTTTA ATTTATATTT TTTCTTTCCC 601 CCTGGCCTTA ACCGAATTTT TTCCCATCGC GATCTAATTC TCCCCCGCTT AATACTGACG 661 CTCTCGCACC CATCTCTCTC CTTCTAGCCT CCGCTAGTCA AAATTTTTGG CGTACTCACC 721 AGTCGCCGCC CCTCGCCTCT TGCCGTGCGC GCTTCANCAA GCCGANTCCT GCGTCGAGAG 781 AGCTCCTCTG GTTTCCCTTT CGCTTTCNAG TCCCNGTTCG GGCGCCACTG CTANAGANTT 841 TCCACACNNN NNNAAAGGGT CTGANGNNTC TCTANTTACC AGANNNNCAC AACANANGGG 901 NNCNCACTAN TNNNANCACT NNANNNNNNN NNTTNNNNNN NNNTNNNNNN NNNGNNTNNC 961 NNNNNNNNNN // |
Primer: SV40pro_F (Pr0110) Region: Zeo Sequence file: RDB20218_B3Keh.seq |
>05736_20218_B3Ke_3_SV40pro_F_H01_22_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNTGNNG NNGCTTTTTT GGNNGCCTAG GCTTTTGCAA 61 AAAGCTCCCG GGANCTTGTA TATCCATTTT CGGATCTGAT CAGCACGTGT TGACAATTAA 121 TCATCGGCAT AGTATATCGG CATAGTATAA TACGACAAGG TGAGGAACTA AACCATGGCC 181 AAGTTGACCA GTGCCGTTCC GGTGCTCACC GCGCGCGACG TCGCCGGAGC GGTCGAGTTC 241 TGGACCGACC GGCTCGGGTT CTCCCGGGAC TTCGTGGAGG ACGACTTCGC CGGTGTGGTC 301 CGGGACGACG TGACCCTGTT CATCAGCGCG GTCCAGGACC AGGTGGTGCC GGACAACACC 361 CTGGCCTGGG TGTGGGTGCG CGGCCTGGAC GAGCTGTACG CCGAGTGGTC GGAGGTCGTG 421 TCCACGAACT TCCGGGACGC CTCCGGGCCG GCCATGACCG AGATCGGCGA GCAGCCGTGG 481 GGGCGGGAGT TCGCCCTGCG CGACCCGGCC GGCAACTGCG TGCACTTCGT GGCCGAGGAG 541 CAGGACTGAC ACGTGCTACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC 601 TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGN TCTCATGCTG 661 GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT 721 AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC 781 NAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCNA NCTCTANCTA GANCTNGGCG 841 TAATCNTGGT CATANCTGTT TCCNGTGNGA AATTGTTATC CNNNCACNAT TCCNCNCNAC 901 NTACNANCNN GNNNNNTAAA NNGNNANNNC NNNGGNNNNC NNNNNGNNNN NNCNNNNNNN 961 NNN // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
user_report | Matsuda, Y., Epigenetic Heterogeneity in HIV-1 Latency Establishment. Sci. Rep. 5: 7701 (2015). PMID 25572573. [link to RRC of NBRP] |
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2024.03.26
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