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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGR RE-TK hRluc(F)

Reporter construct of GR (MR,PR,AR) response element

Catalog number RDB05628
Resource name pGR RE-TK hRluc(F)
Alternative name 2483C7F
Clone info. GR (MR,PR,AR) response element is cloned into the BglII site of phRL-TK, and the BglII site is killed by the cloning. The nucleotide sequence of the GR (MR,PR,AR) response element is 5'-gatccagaacaaaatgttctg-3'.
Comment The nucleotide sequence of the element was determined. Function of the inserted element has not been examined by the depositor.
Vector backbone phRL-TK (Plasmid)
Size of vector backbone 4.0 kb
Selectable markers Amp^r
Gene/insert name Artificial GR (MR,PR,AR) response element synthesized
Depositor|Developer Yokoyama, Kazunari |
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External Database
Artificial GR (MR,PR,AR) response element
            Reference sequence
              
             
            Remarks, protocol and/or map (pdf) RDB05628.pdf

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution The RECIPIENT agrees to expressly describe the acknowledgement of the Gene Engineering Division, RIKEN BRC as the source of the BIOLOGICAL RESOURCE in any publication. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for-profit organization for a not-for-profit research.
            Additional terms and conditions:
            The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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            RDB05628 pGR RE-TK hRluc(F) DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            How to cite this biological resource

            Materials & Methods section:

            The pGR RE-TK hRluc(F) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB05628).

            Reference section:

            Further references such as user reports and related articles (go to bottom)

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            QC test results

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14286_A6H2p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: SV40pA3'Element Reverse
            Sequence file: RDB14286_A6H2a.seq check
            >05628_14286_A6H2_3_SV40pA3ElementReverse_C12_09.ab1
                1 GAGTCTAGGG GGCGGACCTA TGGAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC
               61 TTTTGCTGGC CTTTTGCTCA CATGGCTCGA CAGATCCAGA ACAAAATGTT CTGGATCTAA
              121 ATGAGTCTTC GGACCTCGCG GGGGCCGCTT AAGCGGTGGT TAGGGTTTGT CTGACGCGGG
              181 GGGAGGGGGA AGGAACGAAA CACTCTCATT CGGAGGCGGC TCGGGGTTTG GTCTTGGTGG
              241 CCACGGGCAC GCAGAAGAGC GCCGCGATCC TCTTAAGCAC CCCCCCGCCC TCCGTGGAGG
              301 CGGGGGTTTG GTCGGCGGGT GGTAACTGGC GGGCCGCTGA CTCGGGCGGG TCGCGCGCCC
              361 CAGAGTGTGA CCTTTTCGGT CTGCTCGCAG ACCCCCGGGC GGCGCCGCCG CGGCGGCGAC
              421 GGGCTCGCTG GGTCCTAGGC TCCATGGGGA CCGTATACGT GGACAGGCTC TGGAGCATCC
              481 GCACGACTGC GGTGATATTA CCGGAGACCT TCTGCGGGAC GAGCCGGGTC ACGCGGCTGA
              541 CGCGGAGCGT CCGTTGGGCG ACAAACACCA GGACGGGGCA CAGGTACACT ATCTTGTCAC
              601 CCGGAGGCGC GAGGGACTGC AGGAGCTTCA GGGAGTGGCG CAGCTGCTTC ATCCCCGTGG
              661 CCCGTTGCTC GCGTTTGCTG GCGGTGTCCC CGGAAGAAAT ATATTTGCAT GTCTTTAGTT
              721 CTATGATGAC ACAAACCCCG CCCAGCGTCT TGTCATTGGC GAATTCGAAC ACGCAGATGC
              781 AGTCGGGGCG GCGCGGTCCC AGGTCCACTT CGCATATTAA GGTGACGCGT GTGGCCTCGA
              841 ACACCGAGCG ACCCTGCAGC GACCCGCTTA AAAGCTTGAT TCTTCTGACA CAACAGTCTC
              901 GAACTTAAGC TGCAGAAGTT GGTCGTGAGG CACTGGGCAG GTAAGTATCA AAGGTTACAA
              961 GACAGGTTTA AGGAGACCCA ATAGAAACTG GGCTTGTCGA GACAGGAGAA GACTCTGCGT
             1021 TCTGATAGGC ACCTATGGTC TACTGACATC CACTTGCTTT CTCTCCACAG GTGTCCACCT
             1081 CCCAGTCATA CAGCTCGTAG GCTAGAGTAC CTATTACGAA CTTCACTATA AGCTAGCTCT
            1141 CATCTATGGT CT
            //
            Primer: pAxCALNL_F1
            Sequence file: RDB14286_A6H2b.seq check
            >05628_14286_A6H2_3_pAxCALNL_F1_D12_12.ab1
                1 ATAATCTATC TTATCATGTC TGCTCGAAGC GGCCGCTCTA GAATTACTGC TCGTTCTTCA
               61 GCACGCGCTC CACGAAGCTC TTGATGTACT TACCCATTTC ATCTGGAGCG TCCTCCTGGC
              121 TGAAGTGGAG GCCCTTCACC TTCACGAACT CGGTGTTAGG GAACTTCTTA GCTCCCTCGA
              181 CAATAGCGTT GGAAAAGAAC CCAGGGTCGG ACTCGATGAA CATCTTAGGC AGATCGTCGC
              241 TGGCCCGAAG GTAGGCGTTG TAGTTGCGGA CAATCTGGAC GACGTCGGGC TTGCCTCCCT
              301 TAACGAGAGG GATCTCGCGA GGCCAGGAGA GGGTAGGCCG TCTAACCTCG CCCTTCTCCT
              361 TGAATGGCTC CAGGTAGGCA GCGAACTCCT CAGGCTCCAG TTTCCGCATG ATCTTGCTTG
              421 GGAGCATGGT CTCGACGAAG AAGTTATTCT CAAGCACCAT TTTCTCGCCC TCTTCGCTCT
              481 TGATCAGGGC GATATCCTCC TCGATGTCAG GCCACTCGTC CCAGGACTCG ATCACGTCCA
              541 CGACACTCTC AGCATGGACG ATGGCCTTGA TCTTGTCTTG GTGCTCGTAG GAGTAGTGAA
              601 AGGCCAGACA AGCCCCCCAG TCGTGGCCCA CAAAGATGAT TTTCTTTGGA AGGTTCAGCA
              661 GCTCGAACCA AGCGGTGAGG TACTTGTAGT GATCCAGGAG GCGATATGAG CCATTCCCGC
              721 TCTTGCCGGA CTTACCCATT CCGATCAGAT CAGGGATGAT GCATCTAGCC ACGGGCTCGA
              781 TGTGAGGCAC GACGTGCCTC CACAGGTAGC TGGAGGCAGC GTTACCATGC AGAAAAATCA
              841 CGGCGTTCTC GGCGTGCTTC TCGGAATCAT AGTAGTTGAT GAAGGAGTCC AGCACGTTCA
              901 TTTGCTTGCA GCGAGCCCAC CACTGAGGCC CAGTGATCAT GCGTTTGCGT TGCTCGGGGT
              961 CGTACACCTT GGGAAGCCAT GGTGGCTAGC CTATAGTGAG TCGTATTAAG TACTCTAGCC
             1021 CTTAGAGCTG TAATTGAACT GGGGAGTGGA CACCTGTGGA GAGAAAGCAA AGTGGATGTC
             1081 AGTAGACATT AGTTGCTATC AGAAACGCAG AGTCTTCCTG TCTCGGACAG GCCAGTTCTA
            1141 TTGATCCTCC TTAAACCTGT TCTTTGTGTT AC
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            2023.11.08

            GNP_filter3_RDBDEP_html_231027.pl