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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pKM2L-phPSA

Promoter Bank clone, Human prostate-specific antigen/kallikrein 3 (PSA/KLK3) promoter

Catalog number RDB05546
Resource name pKM2L-phPSA
Alternative name p1965-7F
Clone info. PCR amplified human prostate-specific antigen/kallikrein 3 (PSA/KLK3) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Genomic sequence from 22630 to 23369 of AC011523.3 corresponding -707 to +33 (the transcription start site is designated +1. Fig.1 of Cleutjens, K.B. J. Biol. Chem. 271 (11): 6379-6388, 1996) is cloned. Alternatively, 50854208 to 50854947(NC_000019.10); relatively -707 to +33, where +1 corresponds to 1 nt of NM_001648.2 was cloned.
Comment PCR cloning, forward primer, 1965 F3: 5' agggcaggtcagcaggcatctct 3'; reverse primer, 1965 R3: 5' agctctccgggtgcaggtggtaa 3' Entire sequence of promoter region has not been confirmed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human prostate-specific antigen/kallikrein 3 (PSA/KLK3) genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human prostate-specific antigen/kallikrein 3 (PSA/KLK3)

          Reference sequence
            AC011523, AF243527

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB05546 pKM2L-phPSA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB05546z.seq
          Remarks, protocol and/or map (pdf) RDB05546.pdf

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Renilla Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12756_A4Df.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB12756_A4Dfa.seq check
          >05546_12756_A4Df_M13(-40)_E01_13.ab1
              1 CGGGGCCGGG CCCAGGCTTG CATGCCTGCA GGTCATTTAA TAGGGATAAC AGGGTAATGA
             61 TTAAGATCTC GAGCTCTAAG CTTCACATAT GCATGCACTA GTGGCAGGGC AGGTCAGCAG
            121 GCATCTCTGT TGCACAGATA GAGTGCACAG GTCTGGAGAA CAAGGAGTGG GGGGTTATTG
            181 GAATTCCACA TTGTTTGCTG CACGTTGGAT TTTGAAATGC TAGGGAACTT TGGGAGACTC
            241 ATATTTCTGG GCTAGAGGAT CTGTGGACCA CAAGATCTTT TTATGATGAC AGTAGCAATG
            301 TATCTGTGGA GCTGGATTCT GGGTTGGGAG TGCAAGGAAA AGAATGTACT AAATGCCAAG
            361 ACATCTATTT CAGGAGCATG AGGAATAAAA GTTCTAGTTT CTGGTCTCAG AGTGGTGCAG
            421 GGATCAGGGA GTCTCACAAT CTCCTGAGTG CTGGTGTTTT AGGGCACACT GGGTCTTGGA
            481 GTGCAAAGGA TCTAGGCACG TGAGGCTTTG TATGAAGAAT CGGGGATCAT ACCCACCCCC
            541 TGTTTCTGTT TCATCCTGGG CGTGTCTCCT CTGCCTTTGT CCCCTAGATG AAGTCTCCAT
            601 GAGCTACAGG GCCTGGTGCA TCCAGGGTGA TCTAGTAATT GCAGAACAGC AAGTGCTAGC
            661 TCTCCCTCCC CTTCCACAGC TCTGGGTGTG GGAGGGGGTT GTCCAGCCTC CAGCAGCATG
            721 GGGAGGGCCT TGGTCAGCCT CTGGGTGCCA GCAGGGCAGG GGCGGAGTCC TGGGGAATGA
            781 AGGTTTTATA GGGCTCCTGG GGGAGGCTCC CCAGCCCCAA GCTTACCACC TGCACCCGGA
            841 GAGCTGCCTG TCGACGCGTA GAATTCACCC GGGTACTGCA GAAGTTGGTC GTGAGGCACT
            901 GGGCAGGTAA GTATCAAGGT TACAAGACAG GTTTAAGGAG ACCAATAGAA ACTGGGCTTG
            961 TCGAGACCAG AGAAGACTCT TTGCGTTTCT GATAGGCCAC CTATTGGTCT TACTGAACAT
           1021 CCACTTTTGC CTTTTCTTCT TCCACCAGGT GTCCCACTCC CCAGTTTCAA TTTACAGCTC
           1081 TTAAAGGGCT AAGAAGTTAC CTTTAAATAC GACTTCACTA TCAGGCTTAG CGCAAACAAT
          1141 TGGCACTTCC CCAAAGGTGT ACGAAAC
          //
          Primer: phRLR2
          Sequence file: RDB12756_A4Dfb.seq check
          >05546_12756_A4Df_phRLR2_F01_16.ab1
              1 AACTTCCTGT CTCGACAGCC CAGTTTCTAT TGGTCTCCTT AAACCTGTCT TGTAACCTTG
             61 ATACTTACCT GCCCAGTGCC TCACGACCAA CTTCTGCAGT ACCCGGGTGA ATTCTACGCG
            121 TCGACAGGCA GCTCTCCGGG TGCAGGTGGT AAGCTTGGGG CTGGGGAGCC TCCCCCAGGA
            181 GCCCTATAAA ACCTTCATTC CCCAGGACTC CGCCCCTGCC CTGCTGGCAC CCAGAGGCTG
            241 ACCAAGGCCC TCCCCATGCT GCTGGAGGCT GGACAACCCC CTCCCACACC CAGAGCTGTG
            301 GAAGGGGAGG GAGAGCTAGC ACTTGCTGTT CTGCAATTAC TAGATCACCC TGGATGCACC
            361 AGGCCCTGTA GCTCATGGAG ACTTCATCTA GGGGACAAAG GCAGAGGAGA CACGCCCAGG
            421 ATGAAACAGA AACAGGGGGT GGGTATGATC CCCGATTCTT CATACAAAGC CTCACGTGCC
            481 TAGATCCTTT GCACTCCAAG ACCCAGTGTG CCCTAAAACA CCAGCACTCA GGAGATTGTG
            541 AGACTCCCTG ATCCCTGCAC CACTCTGAGA CCAGAAACTA GAACTTTTAT TCCTCATGCT
            601 CCTGAAATAG ATGTCTTGGC ATTTAGTACA TTCTTTTCCT TGCACTCCCA ACCCAGAATC
            661 CAGCTCCACA GATACATTGC TACTGTCATC ATAAAAAGAT CTTGTGGTCC ACAGATCCTC
            721 TAGCCCAGAA ATATGAGTCT CCCAAAGTTC CCTAGCATTT CAAAATCCAA CGTGCAGCAA
            781 ACAATGTGGA ATTCCAATAA CCCCCCACTC CTTGTTCTCC AGACCTGTGC ACTCTATCTG
            841 TGCAACAGAG ATGCCTGCTG ACCTGCCCTG CCACTAGTGC ATGCATATGT GAAGCTTAGA
            901 GCTCGAGATC TTAATCATTA CCCTGTTATC CCTATTTAAA TGACCTGCAG GCATGCAAGC
            961 TTGGCACTGG CCGTCGTTTT ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT
           1021 AATCGCCTTT GCAGCACATC CCCCTTTCGC CAGCTGGCGT AATAGCGAAG AGGCCCGGCA
           1081 CGATCGCCGT TCCACAGTTG CGCAGCTGAT GGCGATGCCG ATTTATTCCA CCAAAGGCCG
          1141 CCCGATCCCC GTCAAGTTCC AAC
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          reference Cleutjens, K.B., Two androgen response regions cooperate in steroid hormone regulated activity of the prostate-specific antigen promoter. J. Biol. Chem. 271 (11): 6379-6388 (1996). PMID 8626436.

          2022.05.18

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